Resistance of <i>Wolbachia</i> to Trimethoprim: Insights into Genes Encoding Dihydrofolate Reductase, Thymidylate Synthase and Serine Hydroxymethyltransferase in the Rickettsiales by Ann M. Fallon

“…In contrast, DHFR is not encoded by <i>w</i>Fol (supergroup E) and <i>w</i>Bm (supergroup D) or by genomes of the closely related genera <i>Anaplasma</i>, <i>Ehrlichia</i>, <i>Neorickettsia</i>, and possibly <i>Orientia</i>. …”
Get full text

Assessing the Roles of Molecular Markers of Antimalarial Drug Resistance and the Host Pharmacogenetics in Drug-Resistant Malaria by Peter Hodoameda, Nancy Odurowah Duah-Quashie, Neils Ben Quashie

“…Since both host and parasite genomes play a role in antimalarial drug action, a key question often asked is, “which of the two strongly drives or controls antimalarial drug resistance?” …”
Get full text

New Cardiovascular and Pulmonary Therapeutic Strategies Based on the Angiotensin-Converting Enzyme 2/Angiotensin-(1–7)/Mas Receptor Axis by Anderson J. Ferreira, Tatiane M. Murça, Rodrigo A. Fraga-Silva, Carlos Henrique Castro, Mohan K. Raizada, Robson A. S. Santos

Get full text

Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots by Mariam P. Berdeja, Nicole K. Reynolds, Teresa Pawlowska, Justine E. Vanden Heuvel

“…Long-term studies with further genomics, metabolomics and physiological research are needed to provide a deeper understanding of the symbiotic interaction among grapevine roots, bioinoculants and natural AMF communities and their role to promote plant adaptation to current environmental concerns.…”
Get full text

HER2 expression and pathway status in male breast cancer patients: results of an integrated analysis among 6,150 patients by Boqiang Lyu, Shidi Zhao, Hui Wang, Shouping Gong, Biyuan Wang

“…Altered genes in HER2 + MBC, such as ERBB2, AGO2, RECQL4, and CLTC, were not detected in HER2-MBC. Genomic analysis revealed differences between the patients with HER2 + MBC and those with HER2 + FBC. …”
Get full text

Epigenetic modifications in bladder cancer: crosstalk between DNA methylation and miRNAs by Wei Li, Wei Li, Wei Li, Peiyue Luo, Peiyue Luo, Peiyue Luo, Qi Chen, Qi Chen, Qi Chen, Le Cheng, Le Cheng, Le Cheng, Lifeng Gan, Lifeng Gan, Lifeng Gan, Fangtao Zhang, Fangtao Zhang, Fangtao Zhang, Haidong Zhong, Haidong Zhong, Haidong Zhong, Liying Zheng, Biao Qian, Biao Qian

“…Alterations in this methylation pattern can lead to increased genomic instability, which profoundly influences the expression of proto-oncogenes and tumor suppressor genes. …”
Get full text

Regulation of Pear Fruit Quality: A Review Based on Chinese Pear Varieties by Ying Zhang, Yudou Cheng, Yuru Ma, Junfeng Guan, Hao Zhang

“…Key aspects include metabolic regulation of fruit traits such as sweetness, color, texture, and physiological disorders; factors influencing stone cell formation; sugar content regulation; roles of plant hormones including ethylene, gibberellins, and abscisic acid; translational regulation and post-translational modifications such as ubiquitination, methylation, and acetylation; as well as the application of genomic sequencing technologies. Furthermore, the review offers practical suggestions for improving pear fruit quality and provides insights for researchers in related fields. …”
Get full text

Purification, Characterization and Antibacterial Mechanism of Plantaricin 2-1 by Mengyang LI, Xiaoxue CHE, Qingshen SUN

“…Additionally, P2-1 demonstrated the ability to bind to the genomic DNA of the indicator strains, thereby disrupting their DNA structure. …”
Get full text

Machine Learning for Lung Cancer Subtype Classification: Combining Clinical, Histopathological, and Biophysical Features by Aiga Andrijanova, Lasma Bugovecka, Sergejs Isajevs, Donats Erts, Uldis Malinovskis, Andis Liepins

“…Traditional methods like genomic studies face limitations such as high cost and complexity. …”
Get full text

Identification of mitophagy-related biomarkers and immune infiltration in polycystic ovarian syndrome by bioinformatic study by Hongjuan Ye, Xicheng Wang, Zhiying He

“…Gene ontology annotations and Kyoto encyclopedia of genes and genomes signaling pathway enrichment analysis were performed. …”
Get full text

Nucleotide Sequencing and Identification of Some Wild Mushrooms by Sudip Kumar Das, Aninda Mandal, Animesh K. Datta, Sudha Gupta, Rita Paul, Aditi Saha, Sonali Sengupta, Priyanka Kumari Dubey

“…The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned. …”
Get full text

A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei by Mitsuru Nakase, Jeewan Thapa, Vanaabaatar Batbaatar, Ochirbat Khurtsbaatar, Batchuluun Enkhtuul, Jugderkhorloo Unenbat, Baasansuren Lkham, Sachiho Fujita, Ai Koshikawa, Apichai Tuanyok, Vannarat Saechan, Hideaki Higashi, Kyoko Hayashida, Yasuhiko Suzuki, Chie Nakajima, Takashi Kimura

“…Results The primer set targeting the internal transcribed spacer (ITS) region detected 10 genomic copies of B. mallei DNA and B. pseudomallei DNA using the conventional liquid LAMP method. …”
Get full text

Identification of a cryptic unbalanced translocation Der(22)t(12;22)(q24.33;q13.33) in a large Chinese family with Phelan-McDermid syndrome by nanopore sequencing by Xingwu Wu, Qiang Xu, Ge Chen, Jialyv Huang, Yanying Zhong, Lifeng Tian, Qiongfang Wu, Jia Chen

“…According to the guidelines of the American Society for Medical Genetics and Genomics (ACMG), the 22q13.33 microdeletion was classified as pathogenic, whereas the 12q24.33 microduplication was classified as a variant of uncertain significance (VUS). …”
Get full text

CDK9 Expression Shows Role as a Potential Prognostic Biomarker in Breast Cancer Patients Who Fail to Achieve Pathologic Complete Response after Neoadjuvant Chemotherapy by Ashley J. Schlafstein, Allison E. Withers, Soumon Rudra, Diana Danelia, Jeffrey M. Switchenko, Donna Mister, Saul Harari, Hui Zhang, Waaqo Daddacha, Shahrzad Ehdaivand, Xiaoxian Li, Mylin A. Torres, David S. Yu

“…Cyclin-dependent kinase 9 (CDK9) is often dysregulated in breast cancer, and its deficiency results in genomic instability. We reviewed the records of 84 breast cancer patients from Emory University’s Winship Cancer Institute who had undergone surgical resection after NACT and had tissue available for tissue microarray analysis (TMA). …”
Get full text

Isolation and Molecular Identification of Lactic Acid Bacteria Using 16s rRNA Genes from Fermented Teff (Eragrostis tef (Zucc.)) Dough by Belay Tilahun, Anteneh Tesfaye, Diriba Muleta, Andualem Bahiru, Zewdu Terefework, Gary Wessel

“…A total of 24 purified bacterial isolates were found to be Gram-positive (they are coccus and rod under microscope) and were good acid producers. Genomic DNA of bacterial isolates were extracted using Invisorb® Spin DNA Extraction kit. 16S rRNA of bacterial isolates were amplified using the bacteria universal primers (rD1 and fD1). …”
Get full text

How do we measure the costs, benefits, and harms of sharing data from biomedical studies? A protocol for a scoping review [version 2; peer review: 2 approved] by Lauren Maxwell, Ankur Krishnan, Priya Shreedhar

“…Introduction The benefits of sharing participant-level data, including clinical or epidemiological data, genomic data, high-dimensional imaging data, or human-derived samples, from biomedical studies have been widely touted and may be taken for granted. …”
Get full text

De novo assembly and analysis of the transcriptome of the Siberian wood frog Rana amurensis by D. N. Smirnov, S. V. Shekhovtsov, A. A. Shipova, G. R. Gazizova, E. I. Shagimardanova, N. A. Bulakhova, E. N. Meshcheryakova, T. V. Poluboyarova, E. E. Khrameeva, S. E. Peltek, D. I. Berman

“…Little is known about the mechanisms of this remarkable resilience, in part because studies of amphibian genomes are impeded by their large size. To make the Siberian wood frog more amenable for genetic analysis, we performed transcriptome sequencing and de novo assembly for the R. amurensis brain under hypoxia and normoxia, as well as for the normoxic heart. …”
Get full text

Isolation and Characterization of Colistin-Resistant <i>Enterobacteriaceae</i> from Foods in Two Italian Regions in the South of Italy by Rosa Fraccalvieri, Angelica Bianco, Laura Maria Difato, Loredana Capozzi, Laura Del Sambro, Stefano Castellana, Adelia Donatiello, Luigina Serrecchia, Lorenzo Pace, Donatella Farina, Domenico Galante, Marta Caruso, Maria Tempesta, Antonio Parisi

“…The majority of the isolates belonged to <i>Enterobacter</i> spp. (60%), followed by <i>Moellerella wisconsensis</i>, <i>Atlantibacter hermannii</i>, <i>Klebsiella pneumoniae</i>, and <i>Escherichia coli</i>, among others. Genomic sequencing and antimicrobial susceptibility testing revealed high levels of resistance to β-lactams, with most isolates exhibiting multidrug resistance (MDR). …”
Get full text

Phylogenetic analysis of dermatophytes isolated from small domestic animals by A. M. Smagulova, E. V. Kukhar, T. I. Glotova, A. G. Glotov

“…The comparative analysis of the genomes of the Microsporum and Trichophyton fungi and reference strains revealed a relatively low level of intraspecies polymor- phism and point mutations of the sequences. …”
Get full text

Integrating phylogeographic and phenotypic evidence to delimit deep evolutionary lineages in the Dryophytes japonicus species complex, with an assessment of their conservation need... by Amaël Borzée, Yikweon Jang, Siti N. Othman, Jordy Groffen, Irina V. Maslova, Zoljargal Purevdorj, Kiyomi Yasumiba, Tomohiko Shimada, Yoonjung Yi, Natalya Alekseevna Schepina, Desiree Andersen, Yoonhyuk Bae, Ikuo Miura, Christophe Dufresnes

“…In this study, we assessed the genetic, genomic, morphological, and acoustic diversity and differentiation within the D. japonicus complex to propose taxonomic arrangements and assess taxon-specific threats for each defined clade. …”
Get full text