Impact of heteroresistance on treatment outcomes of people with drug-resistant TB by R. Crowder, M. Kato-Maeda, B. Schwem, A. dela Tonga, D.M. Geocaniga-Gaviola, E. Lopez, C.L. Valdez, A.R. Lim, N. Hunat, A.G. Sedusta, C.A. Sacopon, G.A.M. Atienza, E. Bulag, D. Lim, J. Bascuña, K. Shah, R.P. Basillio, C.A. Berger, M.C.D.P. Lopez, S. Sen, C. Allender, M. Folkerts, U. Karaoz, E. Brodie, S. Mitarai, A.M.C. Garfin, M.C. Ama, D.M. Engelthaler, A. Cattamanchi, R. Destura

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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

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Exome sequencing in Nigerian children with early‐onset epilepsy syndromes by Ibitayo Abigail Ademuwagun, Yagoub Adam, Solomon Oladapo Rotimi, Steffen Syrbe, Maximilian Radtke, Julia Hentschel, Johannes R. Lemke, Ezekiel Adebiyi

“…Venous blood samples were collected, and genomic DNA was isolated and purified. Molecular analysis included DNA fragmentation, ligation, target enrichment, library preparation, and whole‐exome sequencing. …”
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Annotation and characterization of immunoglobulin loci and CDR3 polymorphism in water buffalo (Bubalus bubalis) by Yunlan Deng, Fengli Wu, Fengli Wu, Qianqian Li, Lidie Yao, Chengzhi Yang, Long Ma, Xinsheng Yao, Jun Li

“…The CDR3 repertoires were constructed using genomic DNA extracted from spleen samples of five healthy buffaloes. …”
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Proteogenomic annotation of T6SS components identified in Bacteroides fragilis secretome by Natalya B. Zakharzhevskaya, Olga Yu Shagaleeva, Daria A. Kashatnikova, Daria A. Kashatnikova, Anton O. Goncharov, Anton O. Goncharov, Daria V. Evsyutina, Dmitry A. Kardonsky, Elizaveta A. Vorobeva, Artemiy S. Silantiev, Viktoria D. Kazakova, Irina V. Kolesnikova, Ivan O. Butenko, Anna A. Vanyushkina, Svetlana V. Smirnova, Andrei V. Chaplin, Andrei V. Chaplin, Boris A. Efimov, Boris A. Efimov

“…Proteogenomic annotation and comparative genomic study of the T6SS loci of the toxigenic Bf BOB25 were carried out by comparison with the reference genomes of the following Bf strains: JIM10, NCTC 9343 and 638R.ResultsAccording to the data obtained, T6SS components were represented in all types of the analysed samples. …”
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How to convert a 3D printer to a personal automated liquid handler for life science workflows by Dulguunnaran Naranbat, Benjamin Phelps, John Murphy, Anubhav Tripathi

“…The PALH system was evaluated through common life science assays, including preparing real-time PCR samples, end-point PCR with novel pipet-based downstream purification, and genomic DNA extraction from peripheral whole blood. …”
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Refining prognostic assessment of diffuse large B-cell lymphoma: insights from multi-omics and single-cell analysis unveil SRM as a key target for regulating immunotherapy by Xiaojie Liang, Jia Guo, Baiwei Luo, Weixiang Lu, Qiumin Chen, Yeling Deng, Yunong Yang, Liang Wang

“…Mechanisms underlying adverse prognosis in the high-risk DLBCLs were investigated through transcriptomic (n = 3,229), genomic (n = 576), and scRNA-seq (n = 20) analyses. …”
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Russian Science Foundation grant No. 20-14-00140 supported this study. The authors are thankful to the multi-access Center “Bioinformatics” for the use of computational resources a... by O. V. Vishnevsky, I. V. Chadaeva, E. B. Sharypova, B. M. Khandaev, K. A. Zolotareva, A. V. Kazachek, P. M. Ponomarenko, N. L. Podkolodny, D. A. Rasskazov, E. V. Zemlyanskaya, A. G. Bogomolov, O. A. Podkolodnaya, L. K. Savinkova, M. P. Ponomarenko

“…Accordingly, in this paper, albumin, globulin, and β­amylase of common wheat Triticum aestivum L. (1753) are analyzed, which have been identified earlier as targets for attacks by human class E immunoglobulins. At the genomic level, we wanted to find signs of past negative selection against the allergenicity of these three proteins (albumin, globulin, and β­amylase) during the domestication of ancestral forms of modern food plants. …”
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A girl with intragenic variants in MARS2 and a chondrodysplasia phenotype by Hiroyuki Iijima, Yuko Tsujioka, Yoshiyuki Tsutsumi, Gen Nishimura, Yasushi Okazaki, Kei Murayama, Mitsuru Kubota, Akira Ohtake

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Raise and characterization of a bread wheat hybrid line (Tulaykovskaya 10 × Saratovskaya 29) with chromosome 6Agi2 introgressed from Thinopyrum intermedium by Yu. N. Ivanova, K. K. Rosenfread, A. I. Stasyuk, E. S. Skolotneva, O. G. Silkova

“…Chromosome 6Agi2 was identified by PCR with chromosome-specific primers and by genomic in situ hybridization (GISH). According to molecular data, 6Agi2 was transmitted to nearly half of the plants tested in the F2 and F3 generations. …”
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Clinicopathological study and molecular subtyping of muscle-invasive bladder cancer (MIBC) using dual immunohistochemical (IHC) markers by R. Vaithegi, Kanthilatha Pai, Anuradha Calicut Kini Rao, Vidya Monappa, Swathi Prabhu, Nischitha Suvarna

“…As molecular subtyping is complex, surrogate immunohistochemical (IHC) markers have been used to determine the molecular subtypes with good correlation to genomic profiling. Methods We analysed the clinicopathological features of 66 cases of MIBCs received over a 5-year study period. …”
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Association of a Common Variant at 10q26 and Benign Prostatic Hyperplasia Aggressiveness in Han Chinese Descent by Xin Gu, Tao Huang, Ding Xu, Liujian Duan, Yang Jiao, Jian Kang, S. Lilly Zheng, Jianfeng Xu, Jielin Sun, Jun Qi

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Soil Acidobacterial 16S rRNA Gene Sequences Reveal Subgroup Level Differences between Savanna-Like Cerrado and Atlantic Forest Brazilian Biomes by Elisa C. P. Catão, Fabyano A. C. Lopes, Janaína F. Araújo, Alinne P. de Castro, Cristine C. Barreto, Mercedes M. C. Bustamante, Betania F. Quirino, Ricardo H. Krüger

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Protocol of a randomised trial of teriparatide followed by zoledronic acid to reduce fracture risk in adults with osteogenesis imperfecta by Jennifer Walsh, Catriona Keerie, Stuart H Ralston, Christopher J Weir, Muhammad K Javaid, WAYNE LAM, Bente L Langdahl, Jannie D Hald, Patricia Osborne

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Occurrence of Enteric Viruses in Surface Water and the Relationship with Changes in Season and Physical Water Quality Dynamics by Wasonga Michael Opere, Maingi John, Omwoyo Ombori

“…From 216 samples that were analysed for viral contamination, enteric viral genomes were discovered in 18 (8.3%) of the samples. …”
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Tackling ALT-positive neuroblastoma: is it time to redefine risk classification systems? A systematic review with IPD meta-analysis by Marta Avinent-Pérez, Frank Westermann, Samuel Navarro, Amparo López-Carrasco, Rosa Noguera

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Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation by Talke F. zur Bruegge, Andrea Liese, Sören Donath, Stefan Kalies, Maike Kosanke, Oliver Dittrich-Breiholz, Sandra Czech, Verena N. Bauer, André Bleich, Manuela Buettner

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Reply by Denis Vincent, MD, PhD, Ludovic Martin, MD, PhD, Sven Cichon, PhD, Christian Drouet, PharmD, PhD, Arije Ghannam, MD PhD

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Orthopoxvirus Genes That Mediate Disease Virulence and Host Tropism by Sergei N. Shchelkunov

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Successful treatment of life-threatening mycobacteriosis using adjunctive gamma-interferon therapy with genetic analysis by P. Confalonieri, S. Maiocchi, F. Salton, B. Ruaro, C. Rizzardi, M. C. Volpe, D. Licastro, L. Braga, M. Confalonieri

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