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3681
Genetic and Molecular Characterization of Avian Influenza A(H9N2) Viruses from Live Bird Markets (LBM) in Senegal
Published 2025-01-01“…Among these, A/H9N2 was the only subtype detected in both markets, with a detection rate of 47.7% (82/172) in Thiaroye and 35.3% (42/119) in Tilene, resulting in an overall positivity rate of 42.6% (124/291). Genome sequencing of 22 A/H9N2 isolates, including 11 poultry drinking water samples, 7 carcass wash water samples, 3 fecal samples, and 1 cloacal swab, yielded 7 complete and 15 partial genomic sequences. …”
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3682
Mechanisms of blaIMP-4 dissemination across diverse carbapenem-resistant clinical isolates
Published 2025-03-01“…Sequence homology was observed across all plasmids at the blaIMP-4 location, termed Region I, with IS26 on IncHI2A, and IS5075 and Tn3 resistance gene cassettes present on IncC plasmids. Genomic rearrangements mediated by IS26 or Tn3 and IS5075 were identified in Region I of plasmids from the same Inc type. …”
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3683
Preparedness, prevention and control related to zoonotic avian influenza
Published 2025-01-01“…Comprehensive surveillance of AIVs targeting adaptive mutations with whole genome sequencing in animals and humans is essential for early detection of zoonotic AIVs and efficient implementation of control measures. …”
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3684
Co-Infection of <i>Culex tarsalis</i> Mosquitoes with Rift Valley Fever Phlebovirus Strains Results in Efficient Viral Reassortment
Published 2025-01-01“…Furthermore, we observed preferential inclusion of genomic segments from the three parental strains among the reassorted viruses. …”
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3685
Transcriptomic reprogramming and epigenetic regulation underlying pollination-dependent and auxin-induced fruit set in tomato
Published 2025-02-01“…Notably, this developmental process can also proceed in the absence of flower fertilization, although it remains unclear whether pollination-dependent and pollination-independent fruit sets undergo similar transcriptomic reprogramming. Genome-wide transcriptomic profiling of the flower-to-fruit transition, either pollination-induced or triggered by auxin treatment, shows that both types of triggers modulate the expression of a common large set of genes primarily expressed in maternal tissues. …”
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3686
Antimicrobial susceptibility and epidemiological types of Legionella pneumophila human isolates from Italy (1987–2020)
Published 2025-03-01“…The presence of the lpeAB efflux pump genes was also investigated. The genome sequences of a subpopulation of isolates showing reduced susceptibility to azithromycin were also analysed. …”
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3687
SARS-CoV-2 excretion and genetic evolution in nasopharyngeal and stool samples from primary immunodeficiency and immunocompetent pediatric patients
Published 2025-01-01“…RNA detection was performed using RT-qPCR, and whole-genome sequencing was conducted with the NexSeq 1000 platform. …”
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3688
Prophylactic phage administration provides a time window for delayed treatment of vancomycin-resistant Enterococcus faecalis in a murine bacteremia model
Published 2025-01-01“…However, critical gaps remain regarding optimal dosing, therapeutic design, and treatment timing for phage therapy targeting VRE-induced bacteremia.MethodsThe biological and genomic characteristics of a novel lytic phage specific to VRE were investigated. …”
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3689
Neurotrophin-3 as a mediator in the link between PM2.5 exposure and psychiatric disorders: A Mendelian randomization study
Published 2025-01-01“…Background: The causal relationship between PM2.5 (particulate matter with an aerodynamic diameter ≤2.5 μm) and common mental disorders, along with its neuropathological mechanisms, remains unclear. Methods: We used genome-wide association study datasets from the UK Biobank and Psychiatric Genomics Consortium to systematically investigate the causal relationship between PM2.5 and nine common psychiatric disorders using two-sample Mendelian randomization (TSMR) methods. …”
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3690
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3692
Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides
Published 2025-03-01“…The efficiency of mutation was gRNA and Cas9 mRNA concentration dependent, and 400 ng/μl gRNA and 800 ng/μl Cas9 mRNA were recommended as the appropriate injection dose for genome editing in LMB. Enzyme-digestion and sanger-sequencing demonstrated that the CRISPR/Cas9 system is available for the gene editing of LMB. …”
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3693
An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers
Published 2025-02-01“…We aimed to explore the roles of anoikis-related genes (ARGs) in the GI cancer prognosis.MethodsWe extracted RNA sequencing and clinical data from The Cancer Genome Atlas and Gene Expression Omnibu databases for patients with esophageal cancer, gastric cancer, colon cancer and rectal cancer and identified ARGs from GeneCards and Harmonizome. …”
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3694
Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibiti...
Published 2025-01-01“…Methods Using microinjection mRNA and genome engineering to reintroduce the conserved pathogenic missense variants into oocytes or in generating TUBB8 variant knock‐in mouse models, we investigated that the human deleterious variants alter microtubule nucleation and spindle assembly during meiosis. …”
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3695
Dissection of the genetic loci for grain hardness and variation of the key genes in common wheat (Triticum aestivum L.)
Published 2025-03-01“…The genetic loci associated with GHI were dissected based on a high-throughput SNPs map through genome-wide association study (GWAS). Finally, eight QTL, which explained 8.29 % – 14.59 % of the phenotypic variation, were revealed on chromosomes 1 A, 1B, 3 A, 3B, 5 A, 5B and 5D (2). …”
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3696
An Optimized NGS Workflow Defines Genetically Based Prognostic Categories for Patients with Uveal Melanoma
Published 2025-01-01“…Methods: Following the findings published by “The Cancer Genome Atlas–UM” (TCGA-UM) study, we developed an NGS-based gene panel (called the UMpanel) that classifies mutation sets in four categories: initiating alterations (<i>CYSLTR2</i>, <i>GNA11</i>, <i>GNAQ</i> and <i>PLCB4</i>), prognostic alterations (<i>BAP1</i>, <i>EIF1AX</i>, <i>SF3B1</i> and <i>SRSF2</i>), emergent biomarkers (<i>CDKN2A</i>, <i>CENPE</i>, <i>FOXO1</i>, <i>HIF1A</i>, <i>RPL5</i> and <i>TP53</i>) and chromosomal abnormalities (imbalances in chromosomes 1, 3 and 8). …”
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3697
Biallelic SLC13A1 loss-of-function variants result in impaired sulfate transport and skeletal phenotypes, including short stature, scoliosis, and skeletal dysplasia
Published 2025-01-01“…Methods: Probands were identified by exome or genome sequencing and GeneMatcher. Sulfate levels were quantified using ion chromatography. …”
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3698
Microbiology /
Published 2005Table of Contents: “…The history and scope of microbiology -- The study of microbial structure: microscopy and specimen preparation -- Procaryotic cell structure and function -- Eucaryotic cell structure and function -- Microbial nutrition -- Microbial growth -- Control of microorganisms by physical and chemical agents -- Metabolism: energy, enzymes, and regulation -- Metabolism: energy release and conservation -- Metabolism: the use of energy in biosynthesis -- Genes: structure, replication, and mutation -- Genes: expression and regulation -- Microbial recombination and plasmids -- Recombinant DNA technology -- Microbial Genomics -- The viruses: introduction and general characteristics -- The viruses: bacteriophages -- The viruses: viruses of eucaryotes -- Microbial taxonomy -- The archaea -- Bacteria: the deinococci and nonproteobacteria gram negatives -- Bacteria: the proteobacteria -- Bacteria: the Low G 1 C gram positives -- Bacteria: the High G 1 C gram positivies -- The fungi (eumycota), slime molds, and water molds -- The algae -- The protozoa -- Microorganism interactions and microbial ecology -- Microorganisms in aquatic environments -- Microorganisms in terrestrial environments -- Normal microbiota and nonspecific host resistance -- Specific immunity -- Medical immunology -- Pathogenicity of microorganisms -- Antimicrobial chemotherapy -- Clinical microbiology -- The epidemiology of infectious disease -- Human diseases caused by viruses -- Human diseases caused by bacteria -- Human diseases causedd by fungi and protozoa -- Microbiology of food -- Industrial microbiology and biotechnology.…”
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3699
Rare and common single nucleotide variants in childhood-onset systemic lupus erythematosus
Published 2025-02-01“…An SLE common variant PRS was created from 37 SLE genome-wide association study single nucleotide variants (SNVs).Results Rare coding deleterious SNVs (RD SNVs) were observed in 23 of the monogenic SLE-associated genes. …”
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3700
BPZ inhibits early mouse embryonic development by disrupting maternal-to-zygotic transition and mitochondrial function
Published 2025-01-01“…Mechanistically, BPZ exposure inhibits the activation of the zygotic genome and impedes maternal mRNA degradation, thereby interfering with maternal-to-zygotic transition (MZT). …”
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