Refractory human cytomegalovirus infection without evidence of genetic resistance in the UL-54 and UL-97 genes in a pediatric hematopoietic stem cell transplant recipient: a case r... by Alejandra Pando-Caciano, Ketty Adid Escudero-Ramirez, Jackeline Carol Rodríguez-Torres, Holger Maita-Malpartida, Holger Maita-Malpartida

“…Furthermore, this case highlights the importance of using highly sensitive genetic tools to detect mutations associated with virus resistance in a broader range of the viral genome.…”
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Use of a synthetic form Avrodes for transfer of leaf rust resistance from Aegilops speltoides to common wheat by R. O. Davoyan, I. V. Bebyakina, E. R. Davoyan, D. S. Mikov, E. D. Badaeva, I. G. Adonina, E. A. Salina, A. N. Zinchenco, Y. S. Zubanova

“…Hence it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. Introgression lines have been studied for productivity and technological qualities of grain. …”
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Resistance to viruses of potato: current status and prospects by S. S. Makarova, V. V. Makarov, M. E. Taliansky, N. O. Kalinina

“…Recently developed new genome editing technologies with the potential to be a powerful tool for gene design open up broad opportunities for development of next-generation resistance genes. …”
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Splicing accuracy varies across human introns, tissues, age and disease by S. García-Ruiz, D. Zhang, E. K. Gustavsson, G. Rocamora-Perez, M. Grant-Peters, A. Fairbrother-Browne, R. H. Reynolds, J. W. Brenton, A. L. Gil-Martínez, Z. Chen, D. C. Rio, J. A. Botia, S. Guelfi, L. Collado-Torres, M. Ryten

“…We find support for the latter by observing a genome-wide increase in splicing inaccuracies in samples affected with Alzheimer’s disease as compared to neurologically normal individuals. …”
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DNA barcoding: how many earthworm species are there in the south of West Siberia? by S. V. Shekhovtsov, N. E. Bazarova, D. I. Berman, N. A. Bulakhova, E. V. Golovanova, S. V. Konyaev, T. M. Krugova, I. I. Lyubechanskii, S. E. Peltek

“…This method employs short fragments of the genome to identify species, and allows one to work with specimens that cannot be identified by conventional techniques, as well as to search for new species and predict their phylogenetic affinities. …”
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InterTransViewer: a comparative description of differential gene expression profiles from different experiments by А. V. Tyapkin, V. V. Lavrekha, E. V. Ubogoeva, D. Yu. Oshchepkov, N. A. Omelyanchuk, E. V. Zemlyanskaya

“…Meta-analysis of transcriptomic data from different experiments has become increasingly prevalent due to a significantly increasing number of genome-wide experiments investigating gene expression changes under various conditions. …”
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High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South African Neisseria gonorrhoeae Clinical Isolates and Specimens by Nireshni Mitchev, Ravesh Singh, Veron Ramsuran, Arshad Ismail, Mushal Allam, Stanford Kwenda, Florah Mnyameni, Nigel Garrett, Khine Swe Swe-Han, Abraham J. Niehaus, Koleka P. Mlisana

“…Real-time PCR and high-resolution melting analysis were used to detect porA pseudogene (species-specific marker) and resistance-associated targets. Whole-genome sequencing was used as the gold standard for the presence of point mutations. …”
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Taxonomic refinement of Bacillus thuringiensis by Nagham Shiekh Suliman, Reza Talaei-Hassanloui, Hamid Abachi, Sadegh Zarei, Ebrahim Osdaghi

“…By October 2023, whole genome sequences (WGS) of 885 bacterial strains were labeled as B. thuringiensis in the NCBI GenBank database. …”
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In-depth characterization of miRNome in papillary thyroid cancer with BRAF V600E mutation by Azliana Mohamad Yusof, Francis Yew Fu Tieng, Rohaizak Muhammad, Shahrun Niza Abdullah Suhaimi, Isa Mohamed Rose, Sazuita Saidin, Rahman Jamal, Imilia Ismail, Nurul-Syakima Ab Mutalib

“…MiRNAs differentially expressed between BRAF V600E-positive and BRAF V600E-negative PTC tissues were validated in silico using The Cancer Genome Atlas (TCGA) THCA datasets containing 420 samples. …”
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The Genetic Diversity, Population Structure, and Historical Dynamics of Wild <i>Pyrus</i> Species on the Yunnan–Kweichow Plateau by Xiang Yang, Xingguang Dong, Kunming Li, Ying Zhang, Chenxi Xu, Hongliang Huo, Luming Tian, Jiayu Xu, Chao Liu, Dan Qi, Yufen Cao

“…These analyses entailed the collection of 80 accessions, whole-genome resequencing, and the detection of variants including SNPs, InDels, SVs, and CNVs. …”
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Transcriptome profiling revealed multiple circadian rhythm-related genes associated with common gynecological cancers by Lan Peng, Meiping Jiang, Kangming Li, Shuhui Yu, Chunfang Zhao, Lan Zhang, Lan Li

“…BackgroundStudies have shown that more than half of the human genome expression is affected by circadian rhythms, which includes genes involved in cell cycle control, DNA repair and apoptosis that are critical in cancer biology. …”
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Validation of a one-step reverse transcription PCR detection method for Tobamovirus maculatessellati, in tomato (Solanum lycopersicum L.) and pepper (Capsicum annuum L.) by Chellappan Padmanabhan, Andrea Gilliard, Kai-Shu Ling, Yazmín Rivera

“…In this study, we report the validation of a previously described reverse transcription polymerase chain reaction (RT-PCR) assay that amplifies a 289 bp fragment of the coat protein coding region of ToMMV genome. This assay has 100% specificity for ToMMV. …”
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The Identification and Validation of a Robust Immune-Associated Gene Signature in Cutaneous Melanoma by Le-Bin Song, Jiao-Chen Luan, Qi-Jie Zhang, Lin Chen, Hao-Yang Wang, Xue-Chen Cao, Ning-Hong Song, Yan Lu

“…Methods. 434 melanoma patients were extracted from The Cancer Genome Atlas (TCGA) database, and 1811 IAGs were downloaded from the ImmPort database in our retrospective study. …”
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Identification of Ferroptosis-Associated Long Noncoding RNA Prognostic Model and Tumor Immune Microenvironment in Thyroid Cancer by Yongjian Lin, Fu Gan, Xiaoyu He, Huachu Deng, Yong Li

“…The TC lncRNA data were extracted from RNA sequencing files of The Cancer Genome Atlas (TCGA). Then, we performed a two-cluster analysis and grouped 502 patients with TC in a 7 : 3 ratio. …”
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Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR by Ahtisham Fazeel Abbasi, Muhammad Nabeel Asim, Andreas Dengel

“…This groundbreaking technology holds immense potential for the development of targeted therapies for a wide range of diseases, including cancers, genetic disorders, and hereditary diseases. CRISPR-Cas9 based genome editing is a multi-step process such as designing a precise gRNA, selecting the appropriate Cas protein, and thoroughly evaluating both on-target and off-target activity of the Cas9-gRNA complex. …”
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Causal association among smoking, bitter beverage consumption, and risk of osteoporosis: a two-sample mendelian randomization-based study by Yanqian Wu, Jianqian Chao, Min Bao, Na Zhang, Leixia Wang

“…Methods Publicly available genome-wide association study summary data were analyzed using MR methods. …”
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Meta-Analysis of Expression Profiling Data Indicates Need for Combinatorial Biomarkers in Pediatric Ulcerative Colitis by Xinxiu Li, Eun Jung Lee, Danuta R. Gawel, Sandra Lilja, Samuel Schäfer, Huan Zhang, Mikael Benson

“…Unbiased studies using different genome-wide methods have identified a great number of candidate biomarkers for diagnosis and treatment response in pediatric ulcerative colitis (UC). …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…Throughout evolution, anthropogenic Variola virus (VARV) has developed effective mechanisms to overcome human defense reactions. The viral genome encodes soluble proteins imitating the structure of cellular cytokine receptors. …”
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Gene Expression Profiling of IL-17A-Treated Synovial Fibroblasts from the Human Temporomandibular Joint by Toshio Hattori, Naomi Ogura, Miwa Akutsu, Mutsumi Kawashima, Suguru Watanabe, Ko Ito, Toshirou Kondoh

“…Here, we investigated the roles of IL-17A in temporomandibular joint disorders (TMD) using genome-wide analysis of synovial fibroblasts isolated from patients with TMD. …”
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Comparative analysis of the DNA isolated from thyme leaves using different methods by I. V. Bulavin, O. A. Grebennikova, V. A. Brailko, S. A. Feskov, I. V. Mitrofanova

“…Among the tested kits and methods, CTAB + 2% PVP provided thyme DNA suitable for PCR and, presumably, for genome library preparation. The low cost of reagents for this technique makes it applicable for future mass analysis of plant material.…”
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