Egg Microinjection for the Ladybird Beetle Harmonia axyridis by Taro Nakamura, Yuji Matsuoka, Teruyuki Niimi

“…Our step-by-step protocol is applicable to generating transgenic or genome-edited ladybird beetles, facilitating functional genetics in H. axyridis; the microinjection method should be applicable to other insect eggs.…”
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Genetic Contribution to the Pathogenesis of Primary Biliary Cholangitis by Satoru Joshita, Takeji Umemura, Eiji Tanaka, Masao Ota

“…In this review, we describe the influence of HLA alleles and other gene polymorphisms on PBC along with the results of genome-wide association studies (GWAS) on this disease.…”
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Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology by Wangchun Li, U Tim Wu, Yu Cheng, Yanhao Huang, Lipeng Mao, Menghan Sun, Congling Qiu, Lin Zhou, Lijuan Gao

“…Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters. Methods. …”
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Human-Specific Endogenous Retroviruses by Anton Buzdin

“…This review focuses on a small family of human-specific genomic repetitive elements, presented by 134 members that shaped ~330 kb of the human DNA. …”
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Urothelial Cancer Stem Cells by Irena Dimov, Milan Visnjic, Vladisav Stefanovic

“…The rapid development of the CSC field, shoulder to shoulder with powerful genome-wide screening techniques, has provided cause for optimism for the development of more reliable therapies in the future. …”
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metaGE: Investigating genotype x environment interactions through GWAS meta-analysis. by Annaïg De Walsche, Alexis Vergne, Renaud Rincent, Fabrice Roux, Stéphane Nicolas, Claude Welcker, Sofiane Mezmouk, Alain Charcosset, Tristan Mary-Huard

“…The genotypic response to environmental stresses can be investigated through multi-environment trials (METs). However, genome-wide association studies (GWAS) of MET data are significantly more complex than that of single environments. …”
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Deep phenotyping platform for microscopic plant-pathogen interactions by Stefanie Lück, Salim Bourras, Dimitar Douchkov

“…The increasing availability of genetic and genomic resources has underscored the need for automated microscopic phenotyping in plant-pathogen interactions to identify genes involved in disease resistance. …”
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Fatal Hepatocellular Carcinoma in a Patient with Occult Hepatitis B Virus Infection following the Administration of R-CHOP for Diffuse Large B-Cell Lymphoma by Vincenzo Pitini, Massimo Rizzo, Carmela Arrigo, Patrizia Mondello, Giuseppe Altavilla

“…Like overt HBV infection, OBI can be associated with the integration of HBV sequences into the host genome and has a substantial clinical relevance for patients who are severely immunosuppressed for long durations. …”
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Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene editing”, “genome editing”, “CRISPR-Cas systems”, and “CRISPR-associated protein 9”. …”
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iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System by Andrés Javier Orqueda, Carla Alejandra Giménez, Federico Pereyra-Bonnet

“…When Dolly the sheep was born, the first probe into an adult mammalian genome traveling back in time and generating a whole new animal appeared. …”
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Acholeplasma laidlawii PG8 Culture Adapted to Unfavorable Growth Conditions Shows an Expressed Phytopathogenicity by Vladislav M. Chernov, Natalia E. Moukhametshina, Yurii V. Gogolev, Tatiana N. Nesterova, Maxim V. Trushin, Olga A. Chernova

“…The increasing pathogenicity was accompanied by changes in genome expression in these adapted cells. Further studies are needed to explore the exact mechanisms that permit adaptation to unfavorable growth conditions and changes in phytopathogenic potential.…”
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Transmission Electron Microscopy Studies of Cellular Responses to Entry of Virions: One Kind of Natural Nanobiomaterial by Zheng Liu, Shuyu Liu, Jinming Cui, Yurong Tan, Jian He, Jingqiang Zhang

“…The bacteriophage-bacteria interactions are also introduced to elucidate how the bacteriophage conquers the barrier of cell walls in the prokaryotic cells to transport genome into the host.…”
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LncRNAs: New Players in Apoptosis Control by Marianna Nicoletta Rossi, Fabrizio Antonangeli

“…The discovery that the mammalian genome is largely transcribed and that almost half of the polyadenylated RNAs is composed of noncoding RNAs has attracted the attention of the scientific community. …”
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Alternative 3′ UTR polyadenylation is disrupted in the rNLS8 mouse model of ALS/FTLD by Randall J. Eck, Paul N. Valdmanis, Nicole F. Liachko, Brian C. Kraemer

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Higher-Order Chromatin Regulation of Inflammatory Gene Expression by Jin-Wen Xu, Shuang Ling, Jun Liu

“…This article will review emerging evidence on the genome organization hierarchy underlying the inflammatory response.…”
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Colitis in spondyloarthritis by Muhammed Hafis, Vikas Agarwal

“…Apart from the strong Class I human leukocyte antigen B27 link for both diseases, several new shared genetic risk loci are identified from genome-wide association studies. Demonstrable mucosal inflammation of gut is present in most of the patients with SpA, even in the absence of clinically overt IBD. …”
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Glioblastoma Multiforme Stem Cells by Irena Dimov, Deasanka Tasic-Dimov, Irena Conic, Vladisav Stefanovic

“…The rapidly emerging focus on cancer stem cells embodies a paradigm shift in our understanding of tumor pathogenesis, while the development of powerful genome-wide screening techniques has provided cause for optimism related to the development of more reliable therapies primarily targeting GBM stem cells (GBMSCs). …”
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ncRNA-Mediated High Expression of LPCAT1 Correlates with Poor Prognosis and Tumor Immune Infiltration of Liver Hepatocellular Carcinoma by Qiu Sun, Xudong Liu, Qunlong Peng, Lei Hu, Xiaochun Jiang

“…In this study, expression analysis and survival analysis for LPCAT1 in pan cancers were first performed by using The Cancer Genome Atlas (TCGA) data, which suggested that LPCAT1 might be a potential LIHC oncogene. …”
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The dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans. by Satya Dev Polisetty, Krishna Bhat, Kuladeep Das, Ivan Clark, Kevin G Hardwick, Kaustuv Sanyal

“…Timely activation and maintenance of the SAC until defects are corrected is essential for genome stability. Here, we show that shugoshin (Sgo1), a conserved tension-sensing protein, ensures the maintenance of SAC signals in response to unattached kinetochores during mitosis in a basidiomycete budding yeast Cryptococcus neoformans. …”
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Leber's Hereditary Optic Neuropathy-Gene Therapy: From Benchtop to Bedside by Rajeshwari D. Koilkonda, John Guy

“…These mutations are located at nucleotide positions 3460, 11778, or 14484 in the mitochondrial genome. The disease is characterized by apoplectic, bilateral, and severe visual loss. …”
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