Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome by Hugh D. Goold, Heinrich Kroukamp, Paige E. Erpf, Yu Zhao, Philip Kelso, Julie Calame, John J. B. Timmins, Elizabeth L. I. Wightman, Kai Peng, Alexander C. Carpenter, Briardo Llorente, Carmen Hawthorne, Samuel Clay, Niël van Wyk, Elizabeth L. Daniel, Fergus Harrison, Felix Meier, Robert D. Willows, Yizhi Cai, Roy S. K. Walker, Xin Xu, Monica I. Espinosa, Giovanni Stracquadanio, Joel S. Bader, Leslie A. Mitchell, Jef D. Boeke, Thomas C. Williams, Ian T. Paulsen, Isak S. Pretorius

“…Abstract The Sc2.0 global consortium to design and construct a synthetic genome based on the Saccharomyces cerevisiae genome commenced in 2006, comprising 16 synthetic chromosomes and a new-to-nature tRNA neochromosome. …”
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Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain by Jinling HUANG, Yushuang WU, Huiquan TANG, Minyi HUANG, Ruixue YANG, Qiang WANG, Yong YANG, Xiaodan TAN

“…【Method】Through whole-genome analysis of R. solanacearum PeaFJ1 strain, the insertion sites for exogenous gene were selected. …”
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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…ABSTRACT The bacterium responsible for Lyme disease, Borrelia burgdorferi, accumulates high levels of manganese without iron and possesses a polyploid genome, characteristics suggesting potential extreme resistance to radiation. …”
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Author Correction: AIVariant: a deep learning-based somatic variant detector for highly contaminated tumor samples by Hyeonseong Jeon, Junhak Ahn, Byunggook Na, Soona Hong, Lee Sael, Sun Kim, Sungroh Yoon, Daehyun Baek

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Telomere DNA in the insect order Dermaptera and the first evidence for the non-canonical telomeric motif TTCGG in Arthropoda by Vladimir A. Lukhtanov

“…In this study, analysis of the chromosome-level genome assembly shows that the telomeric DNA of the earwig Labia minor (Linnaeus, 1758) (Polyneoptera, Dermaptera, Spongiphoridae) consists of repeats of the 5 bp motif TTCGG/CCGAA. …”
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Dysregulation of transposable elements and PIWI-interacting RNAs in myelodysplastic neoplasms by Zdenek Krejcik, David Kundrat, Jiri Klema, Andrea Hrustincova, Iva Trsova, Monika Belickova, Jaroslav Cermak, Anna Jonasova, Jiri Dostal, Michaela Dostalova Merkerova

“…Abstract Background Myelodysplastic neoplasms (MDS) are heterogeneous hematopoietic disorders characterized by ineffective hematopoiesis and genome instability. Mobilization of transposable elements (TEs) is an important source of genome instability leading to oncogenesis, whereas small PIWI-interacting RNAs (piRNAs) act as cellular suppressors of TEs. …”
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Selection of promising apple genotypes for columnar growth habit and scab resistance using diagnostic DNA markers by N. I. Savel’ev, A. S. Lyzhin, N. N. Savel’eva

“…The use of molecular markers very accurately determines the presence of necessary genes in the genome early during ontogenesis and reduces the time of selection. …”
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Evaluation of various RNA-seq approaches for identification of gene outrons in the flatworm Opisthorchis felineus by N. I. Ershov, D. E. Maslov, N. P. Bondar

“…Recently, we assembled the O. felineus genome, but the correct genome annotation by means of standard methods was hampered by the presence of spliced leader trans-splicing (SLTS). …”
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From one size fits all to a tailored approach: integrating precision medicine into medical education by Lana Sbitan, Noor Alzraikat, Haneen Tanous, Abdelrahman M. Saad, Mohanad Odeh

“…Integrating precision medicine and genome-based technologies into medical education is necessary for its successful application in clinical practice.…”
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Tissue-specific roles of de novo DNA methyltransferases by Dániel Márton Tóth, Flóra Szeri, Mária Ashaber, Muhyiddeen Muazu, Lóránt Székvölgyi, Tamás Arányi

“…Abstract DNA methylation, catalyzed by DNA methyltransferases (DNMT), plays pivotal role in regulating embryonic development, gene expression, adaption to environmental stress, and maintaining genome integrity. DNMT family consists of DNMT1, DNMT3A, DNMT3B, and the enzymatically inactive DNMT3L. …”
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Internal transcribed spacer metagenomics data unravelling the core fungal community structure residing the wheat and maize rhizosphereNCBINCBINCBI by Sadia Latif, Rizwana Kousar, Anum Fatima, Naeem Khan, Hina Fatimah

“…Plants are colonized by a vast array of microorganisms that outstrip plant cell densities and genes, thus referred to as plant's second genome or extended genome. The microbial communities exert a significant influence on the vigor, growth, development and productivity of plants by supporting nutrient acquisition, organic matter decomposition and tolerance against biotic and abiotic stresses such as heat, high salt, drought and disease, by regulating plant defense responses. …”
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The clinical value and most informative threshold of polygenic risk score in the Quebec City Case-Control Asthma Cohort by Martin Pariès, Stéphanie Bougeard, Aida Eslami, Zhonglin Li, Michel Laviolette, Louis-Philippe Boulet, Evelyne Vigneau, Yohan Bossé

“…Abstract Genome-wide association studies (GWAS) have identified genetic variants robustly associated with asthma. …”
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Clostridium tetani bacteraemia in the plague area in France: Two cases by M.A. Boualam, A. Bouri, M. Signoli, M. Drancourt, A. Caputo, E. Terrer, G. Aboudharam

“…Colonies were identified by mass spectrometry and whole genome sequencing, and C. tetani-specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. …”
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Ribosome pausing in amylase producing Bacillus subtilis during long fermentation by Yaozu Han, Biwen Wang, Alberto Agnolin, Gaurav Dugar, Frans van der Kloet, Christopher Sauer, Paul Igor Costea, Max Fabian Felle, Mathis Appelbaum, Leendert W. Hamoen

“…After achieving single codon resolution for ribosome pausing, we determined the genome wide ribosome pausing sites for B. subtilis at 16 h and 64 h growth under batch fermentation. …”
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Liquid biopsy approaches to capture tumor evolution and clinical outcomes during cancer immunotherapy by Susan Scott, Mark Sausen, Valsamo Anagnostou, Jennifer Jackson, Vincent Lam, Joseph C Murray, Lavanya Sivapalan, Jenna Vanliere Canzoniero, Blair Landon, Benjamin P. Levy

“…Tracking the landscape of genomic alterations detected in ctDNA may reveal the clonal architecture of the metastatic cascade and thus improve our understanding of the molecular wiring of therapeutic responses. …”
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GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma by Zhao-shou Yang, Qin Yang, Xiao-xiao Sun, Kui Xiong, Xiao-ting Zhu, Yi-chen Wang, Qian-yao Ren, Guo-hui Wu, Shi-min Wang, Xu-qin Cao, Xiao-rong Yang, Wen-gong Jiang

“…Here, we leveraged Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) database to investigate the association between GPX8 and overall survival (OS) of patients with primary gliomas, and our results showed that GPX8 expression was negatively correlated with OS. …”
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Efficient chimeric mouse production using a novel embryonic stem cell line by G. V. Kontsevaya, N. A. Feofanova, A. G. Menzorov, I. E. Pristyazhnyuk, A. V. Smirnov, N. R. Battulin, L. A. Gerlinskaya

“…Never-theless, there is a need for complex genome modifications, such as large deletions, reporter genes insertion into the 3’ gene regulatory sequence, or site-specific modifications of the genome. …”
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The interphase period “germination–heading” of 8x and 6x triticale with different dominant Vrn genes by P. I. Stepochkin, A. I. Stasyuk

“…This genome is absent in the winter 6x triticale ‘Sears 57’. …”
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Xanthohumol and echinocystic acid induces PSTVd tolerance in tomato by Wenkun Tang, Zhichao Tang, Haiyi Liu, Jinbiao Lu, Qianyun Du, Huan Tian, Jingwei Li

“…We obtained the full‐length genome sequence of previously conserved PSTVd and inoculated it on four genotypes of semi‐cultivated tomatoes selected from a local tomato germplasm resource. …”
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African swine fever in the Primorsky Krai: disease situation and molecular and biological properties of the isolate recovered from a wild boar long bone by А. R. Shotin, A. S. Igolkin, Ali Mazloum, I. V. Shevchenko, N. S. Bardina, E. O. Morozova, А. А. Shevtsov

“…The incubation period and duration of the disease course in experimentally infected pigs were 4–6 and 3–5 days post infection, respectively. The ASFV genome was detected in blood samples collected from infected pigs on 5–8 days post infection by real-time polymerase chain reaction. …”
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