MEK Inhibition Targets Cancer Stem Cells and Impedes Migration of Pancreatic Cancer Cells In Vitro and In Vivo by Karolin Walter, Kanishka Tiwary, Marija Trajkovic-Arsic, Ana Hidalgo-Sastre, Laura Dierichs, Sven T. Liffers, Jiangning Gu, Johann Gout, Lucas-Alexander Schulte, Jan Münch, Thomas Seufferlein, Bruno Sainz, Jens T. Siveke, Eva Rodriguez-Aznar, Patrick C. Hermann

“…Due to their high dependency on Ras mutations, pancreatic cancers might be particularly sensitive to inhibitors acting downstream of Ras. Herein, we use a genetically engineered mouse model of pancreatic cancer and primary pancreatic cancer cells were derived from this model to demonstrate that small-molecule MEK inhibitors functionally abrogate cancer stem cell populations as demonstrated by reduced sphere and organoid formation capacity. …”
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Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering by William S. Grubbe, Bixia Zhang, Aileen Kauffman, Fabian Byléhn, Kasia Padoł, Hae-Gwang Jung, Seung Bum Park, Jessica M. Priest, Engin Özkan, Juan J. de Pablo, T. Jake Liang, Minglei Zhao, Juan L. Mendoza

“…Abstract IFNλ4 has posed a conundrum in human immunology since its discovery in 2013, with its expression linked to complications with viral clearance. While genetic and cellular studies revealed the detrimental effects of IFNλ4 expression, extensive structural and functional characterization has been limited by the inability to express and purify the protein, complicating explanations of its paradoxical behavior. …”
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Coexistence of Factor VII Deficiency and Hereditary Spastic Paraplegia in Two Siblings by Hortensia De la Corte-Rodriguez, E. Carlos Rodriguez-Merchan, M. Teresa Alvarez-Roman, Ana L. Hernandez-Moreno

“…This disease was also inherited in an autosomal recessive pattern with no direct genetic association with factor VII deficiency. Neurological symptoms had gone unnoticed due to a history of multiple joint haemarthrosis; musculoskeletal examination led to a satisfactory differential diagnosis. …”
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Prevention of transgenerational transmission of disease susceptibility through perinatal intervention by Takahiro Nemoto, Norimasa Sagawa

“…This review thus outlines the mechanisms underlying various disease risk formations and their genetic programs for the next generation, which are being elucidated through studies based on our fetal undernutrition rat models.…”
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Occurrence of macrolides resistance in Legionella pneumophila ST188: Results of the Belgian epidemiology and resistome investigation of clinical isolates by Charlotte Michel, Fedoua Echahidi, Geraldine De Muylder, Max Sewell, Ian Boostrom, Olivier Denis, Owen B. Spiller, Denis Pierard

“…Genotypic screening identified a 23S ribosomal RNA mutation linked to a high-level macrolide resistance in one isolate of ST188, which is genetically closed to resistant isolates from France. …”
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Another new karst-dwelling rock gecko in the Cnemaspis siamensis group (Reptilia, Gekkonidae) from Kanchanaburi Province, western Thailand by Attapol Rujirawan, Akrachai Aksornneam, Siriporn Yodthong, Parinya Pawangkhanant, Bryan L. Stuart, Anchalee Aowphol

“…Phylogenetically, the new species is recovered as the sister taxon to C. huaseesom, but the two species are separated by 8.3–9.4% uncorrected pairwise genetic divergences in the mitochondrial NADH dehydrogenase subunit 2 gene and flanking tRNAs.…”
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Chemogenomic Screening in a Patient‐Derived 3D Fatty Liver Disease Model Reveals the CHRM1‐TRPM8 Axis as a Novel Module for Targeted Intervention by Sonia Youhanna, Aurino M. Kemas, Shane C. Wright, Yi Zhong, Britta Klumpp, Kathrin Klein, Aikaterini Motso, Maurice Michel, Nicole Ziegler, Mingmei Shang, Pierre Sabatier, Aimo Kannt, Hongda Sheng, Nuria Oliva‐Vilarnau, Florian A. Büttner, Brinton Seashore‐Ludlow, Jonas Schreiner, Maike Windbergs, Martin Cornillet, Niklas K. Björkström, Andreas J. Hülsmeier, Thorsten Hornemann, Jesper V. Olsen, Yi Wang, Roberto Gramignoli, Michael Sundström, Volker M. Lauschke

“…Among these, activation of the muscarinic M1 receptor (CHRM1) and inhibition of the TRPM8 cation channel result in strong anti‐fibrotic effects, which are confirmed using orthogonal genetic assays. Strikingly, using biosensors based on bioluminescence resonance energy transfer, a functional interaction along a novel MASH signaling axis in which CHRM1 inhibits TRPM8 via Gq/11 and phospholipase C‐mediated depletion of phosphatidylinositol 4,5‐bisphosphate can be demonstrated. …”
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Mitochondrial genome of Cordyceps blackwelliae: organization, transcription, and evolutionary insights into Cordyceps by Yong-Jie Zhang, Xiang-Ping Fan, Jia-Ni Li, Shu Zhang

“…Different mitochondrial protein-coding genes showed variable degrees of genetic differentiation among these species, but they were all under purifying selection. …”
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Whole exome sequencing identifies genomic alterations in proximal and distal colorectal cancer by Ryia-Illani Mohd Yunos, Nurul-Syakima Ab Mutalib, Sheau Sean Khor, Sazuita Saidin, Mohd Ridhwan Abd Razak, Norshahidah Mahamad Nadzir, Zuraini Abd. Razak, Isa Mohamed Rose, Ismail Sagap, Luqman Mazlan, Nadiah Abu, Rahman Jamal

“…Little is known about the relationship between the genetic landscape and the anatomical location of the tumour; as well as the prognostication in CRC patients. …”
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Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review by Raghavendra B. Narasimha, Singireddy Shreya, Vijay Anand Jayabal, Vikas Yadav, Prasana Kumar Rath, Bidyut Prava Mishra, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty, Manoj Kumar Jena

“…In addition to the diseases mentioned, the potential of stem cells can be helpful in wound healing, skin disease therapy, and treatment of some genetic disorders. This article explores the potential of stem cells from various sources in the therapy of livestock diseases and also their role in the conservation of endangered species as well as disease model preparation. …”
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Evaluation Of Impulsivity Symptoms And Family Functioning In Adolescents Diagnosed With Obsessive Compulsive Disorder And Their Mothers by Gonca Ozyurt

“…Further studies are needed to examine genetic and enviromental common risk factors between OCD and impulsivity [JCBPR 2016; 5(2.000): 57-64]…”
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Winter tick sharing between ungulates in the Greater Yellowstone Ecosystem and implications for apparent competition by Troy Koser, Alynn Martin, Alyson Courtemanch, Laura Thompson, Benjamin Wise, Gary Fralick, Sarah Dewey, Amy Girard, Brandon Scurlock, Jared Rogerson, Kennan Oyen, Paul Cross

“…Our analysis of 2793 informative single‐nucleotide polymorphisms showed genetic differentiation among tick populations that were only ~75 km apart, but fewer differences among ticks from elk or moose in the same locality, suggesting sharing of winter ticks across host species. …”
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Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKKβ-AMPK-SIRT1 Signaling Pathway by Xin Liu, Ning Wang, Yuanfeng Zhu, Yongjun Yang, Xiaoli Chen, Shijun Fan, Qian Chen, Hong Zhou, Jiang Zheng

“…Then, withdrawal of extracellular calcium was found to suppress CaMKKβ and AMPK activation triggered by LPS while chemical inhibition or genetic knockdown of these two kinases augmented LPS induced IL-12 production. …”
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Footprint of the COVID-19 Pandemic in India: A Study of Immune Landscape and Other Factors Shielding Mortality by Noura Al-Dayan, Divya Venugopal, Sugapriya Dhanasekaran

“…India being a highly populated nation has so far effectively counteracted the pandemic outbreak with a significantly lower rate of mortality despite the high infection rates. The genetic architecture of the immune response genes in the Indian population, BCG vaccination, the predominantly young age group of people, and their traditional food habits might contribute to the lower rate of mortality. …”
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Enhancing Stock Portfolio Selection with Trapezoidal Bipolar Fuzzy VIKOR Technique with Boruta-GA Hybrid Optimization Model: A Multicriteria Decision-Making Approach by Sunil Kumar Sharma

“…The Boruta-GA approach combines the advantages of the Genetic Algorithm (GA) and Boruta Optimization Algorithm (BOA) methods, implementing the comprehensive feature selection capability of Boruta to detect all relevant features and harnessing the strength of GA to bring about improvement within a wide range of datasets. …”
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Influence of Second-Trimester Ultrasound Markers for Down Syndrome in Pregnant Women of Advanced Maternal Age by Mariza Rumi Kataguiri, Edward Araujo Júnior, Luiz Claudio Silva Bussamra, Luciano Marcondes Machado Nardozza, Antonio Fernandes Moron

“…This was a retrospective cohort study on 889 singleton pregnancies between the 14th and 30th weeks, with maternal age ≥ 35 years, which would undergo genetic amniocentesis. The second-trimester ultrasound assessed the following markers: increased nuchal fold thickness, cardiac hyperechogenic focus, mild ventriculomegaly, choroid plexus cysts, uni- or bilateral renal pyelectasis, intestinal hyperechogenicity, single umbilical artery, short femur and humerus length, hand/foot alterations, structural fetal malformation, and congenital heart disease. …”
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Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis by Caicai Lin, Shuangshuang Zheng, Kui Liu, Ru Yu, Peiyan Guan, Baigeng Hu, Lingling Jiang, Mengyu Su, Guodong Hu, Qingshuai Chen, Xia Zhang

“…The findings also lay the foundation for studying molecular mechanisms of salt tolerance and advancing genetic breeding efforts to cultivate more resilient potato varieties.…”
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Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy by Claudio Azzolini, Ilaria Stefania Pagani, Cristina Pirrone, Davide Borroni, Simone Donati, Muna Al Oum, Diana Pigni, Anna Maria Chiaravalli, Riccardo Vinciguerra, Francesca Simonelli, Giovanni Porta

“…We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.…”
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In Silico Analysis of Common Long Noncoding RNAs in Schistosoma mansoni and Schistosoma haematobium by Serhat Sirekbasan, Tugba Gurkok Tan

“…Schistosomiasis caused by Schistosoma parasites is one of the most common parasitic infections worldwide. Genetic regulation of the genus Schistosoma, which has different developmental stages throughout its life, is quite complex. …”
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Multi-objective optimization of water resources allocation in rift valley lakes basin (Ethiopia): tradeoffs between efficiency and equity by Mulugeta Mohammed, Belete Birhanu, Fentaw Abegaz

“…This study introduces a multi-objective optimization framework using the Non-Dominated Sorting Genetic Algorithm II (NSGA-II) to balance economic efficiency, equity, and environmental sustainability in water allocation. …”
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