Calcitonin and Bone Physiology: In Vitro, In Vivo, and Clinical Investigations by Jingbo Xie, Jian Guo, Zaeema Kanwal, Mingzheng Wu, Xiangyang Lv, Nihal Abdalla Ibrahim, Ping Li, Manal Ali Buabeid, El-Shaimaa A. Arafa, Qingshan Sun

“…The recent research studies carried out on genetically modified mice showed the inhibition of osteoclast activity by amylin, while astonishingly calcitonin plays its role by suppressing osteoblast and bone turnover. …”
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Postharvest wounding treatment influences flavone production and biological activities of the hairy root cultures of Scutellaria baicalensis by Hyeon Ji Yeo, Su Young Shin, Sang Un Park, Chang Ha Park

“…Results In this study, we obtained hairy roots, which are considered natural genetically modified organisms, from S. baicalensis leaves in vitro using wild Agrobacterium rhizogenes (A. rhizogenes) R1000 and investigated the effects of postharvest wounding treatment on their flavone production and in vitro antimicrobial properties. …”
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Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation by Li Chen, Kaikai Shi, Weimin Qiu, Lars Aagaard, Moustapha Kassem

“…However, mechanistic studies employing hMSC have been hampered by the difficulty of deriving genetically modified cell lines due to the low and unstable transfection efficiency. …”
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Animal Models for Human-Pathogenic Coronavirus and Animal Coronavirus Research by Fenglian Xiao, Jincheng Hu, Minsheng Xu, Di Wang, Xiaoyan Shen, Hua Zhang, Jie Miao, Haodong Cai, Jihui Wang, Yaqing Liu, Shan Xiao, Longchao Zhu

“…These approaches include using viral delivery methods to induce the expression of viral receptors in mouse tissues and employing gene-editing techniques to create genetically modified mice. This has led to the successful establishment of infection models for multiple coronaviruses, significantly advancing related research. …”
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CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots by Maria A. Lebedeva, Maria S. Gancheva, Maksim R. Losev, Sofia V. Sokornova, Oleg S. Yuzikhin, Anna A. Krutikova, Kirill V. Plemyashov, Lyudmila A. Lutova

“…According to HPLC-IT-TOF/MS analysis, the levels of orobanchol were decreased in the genetically modified roots with knock-out of the <i>HaNSP1a</i> gene, whereas, in contrast, 5-deoxystrigol levels were increased in the roots with <i>HaNSP1a</i> knock-out, suggesting the role of <i>HaNSP1a</i> in the regulation of the strigolactone biosynthetic pathway. …”
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Recent advances in the application of Mendelian randomization to chronic kidney disease by Chaofan Wen, Lanlan Chen, Dan Jia, Ziqi Liu, Yidan Lin, Guan Liu, Shuo Zhang, Baoshan Gao

“…New insights also reveal a substantial causal connection between both central and general obesity and CKD onset, while no significant links were found between genetically modified LDL cholesterol or triglyceride levels and kidney function.Conclusion This review not only presents the recent achievements of MR in CKD research but also illuminates the path forwards, underscoring critical unanswered questions and proposing future research directions in this dynamic field.…”
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Closed-loop systems for plants expressing animal proteins: a modernized framework to safeguard the future of agricultural innovation by Kristin A. Bresnahan, Justin M. Ferber, J. Thomas Carrato, Thomas J. Stoddard, Patrick V. Palad, Magi Richani

“…Plant molecular farming (PMF), which uses genetically modified (GM) plants to produce high-value proteins for food products, offers a promising solution. …”
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Analysis of population structure and genetic diversity in a Southern African soybean collection based on single nucleotide polymorphism markers by A. Tsindi, J. S. Y. Eleblu, E. Gasura, H. Mushoriwa, P. Tongoona, E. Y. Danquah, L. Mwadzingeni, M. Zikhali, E. Ziramba, G. Mabuyaye, J. Derera

“…Rapid phenotyping revealed grain yield potential ranging from one to five tons per hectare for the 200 non-genetically modified accessions. Findings from this study will inform the crossing strategy for the subtropical soybean breeding programs. …”
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Constructing the constitutively active ribosomal protein S6 kinase 2 from <i>Arabidopsis thaliana</i> (AtRPS6K2) and testing its activity <i>in vitro</i> by A. V. Zhigailov, G. E. Stanbekova, D. K. Beisenov, A. S. Nizkorodova, N. S. Polimbetova, B. K. Iskakov

“…Besides, such an approach has a biotechnological application in producing genetically modified plants with increased biomass and productivity through stimulation of cell growth and division.…”
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Preparation and characterization of new silica-based heterofunctional biocatalysts utilizing low-cost lipase Eversa® Transform 2.0 and evaluation of their catalytic performance in... by Wagner C. A. Carvalho, Rayane A. S. Freitas, Milson S. Barbosa, Ariela V. Paula, Ernandes B. Pereira, Adriano A. Mendes, Elton Franceschi, Cleide M. F. Soares

“…This work offers the opportunity to understand and synthesize new biocatalysts with a low-cost genetically modified lipase using a renewable raw material, opening new possibilities to fill gaps that still exist in the use of lipases for biolubricant production.…”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…Intravenous delivery of genetically modified MSCs overexpressing CXCR4 with a Fluc2 reporter gene may be a useful, noninvasive BLI imaging tool for tracking cell fate.…”
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Government and farmer responses to the fall armyworm outbreak in mainland Southeast Asia by Eiichi Kusano, Nipon Poapongsakorn, Urairat Jantarasiri, Kamphol Pantakua, Cuong H. Tran, Khin Akari Tar, Khin Akari Tar, Thong Kong, Viengsavanh V. Phimphachanhvongsod, Youichi Kobori

“…Alternatives to foliar treatment with chemical insecticides, mainly EMB, such as seed treatment with diamides and neonicotinoids, genetically modified maize seeds approved only in Vietnam, and biological control, have demonstrated efficacy. …”
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Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment by Li Zhang, Wenjing Shen, Zhixiang Fang, Laipan Liu, Ruizong Jia, Jingang Liang, Biao Liu

“…The cultivation of genetically modified (GM) maize has aroused notable public concern related to the potential risks to soil fauna caused by the release of foreign proteins. …”
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CDK14 regulates the development and repair of lung by Jian-wei Chen, Yu-xiang Wang, Rong-rong Gao, Lan-yue Ma, Jing Zhong, Jia-xin Yang, Zhao-hua Deng, Yu-yan Li, Xiao-ling Li, Ya-hai Shu, Wen-jing Guo, Zi-yuan Zhou, Xiao Yu Tian, Jinjin Ma, Yang Liu, Qi Chen

“…However, the function of CDK14 during organ development and regeneration has not been investigated in genetically-modified animals. Here, we found that genetic ablation of Cdk14 influenced pulmonary vascular endothelial cells and alveolar epithelial cells during mice embryonic development as well as repair of lung after bleomycin or lipopolysaccharide induced injury. …”
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<i>Ex Vivo</i> Regional Gene Therapy Compared to Recombinant BMP-2 for the Treatment of Critical-Size Bone Defects: An In Vivo Single-Cell RNA-Sequencing Study by Arijita Sarkar, Matthew C. Gallo, Jennifer A. Bell, Cory K. Mayfield, Jacob R. Ball, Mina Ayad, Elizabeth Lechtholz-Zey, Stephanie W. Chang, Osamu Sugiyama, Denis Evseenko, Jay R. Lieberman

“…<i>Ex vivo</i> regional gene therapy is a promising tissue-engineering strategy for bone regeneration: osteogenic mesenchymal stem cells (MSCs) can be genetically modified to express an osteoinductive stimulus (e.g., bone morphogenetic protein-2), seeded onto an osteoconductive scaffold, and then implanted into a bone defect to exert a therapeutic effect. …”
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Eu:CROPIS AIV Program: Challenges and Solutions for a Spin-Stabilized Satellite Containing Biology by Toni Delovski, Catherin Düvel, Fabian Greif, Ansgar Heidecker, Sebastian Kottmeier, Olaf Mierheim, Falk Nohka, Fabian Orlowski-Feldhusen

“…A secondary payload (“PowerCells”) by the NASA Ames Research Center also utilizes the artificial gravity to conduct growth experiments on genetically modified organisms (GMOs). These payloads and the system design imposed constraints which affected the Assembly Integration and Verification (AIV) program in various ways and created challenges for the relatively small team to find solutions for. …”
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CD94-driven in vitro expansion of highly functional adaptive NKG2C+ NKG2A- CD57+ NK cells from CMV+ healthy donors by Chiara Giordano, Simona Carlomagno, Simona Carlomagno, Michela Falco, Claudia Cantoni, Claudia Cantoni, Massimo Vitale, Ignazio Caruana, Johannes Dirks, Alberto Serio, Letizia Muccio, Giulia Bartalucci, Alessandra Bo, Franco Locatelli, Franco Locatelli, Cristina Bottino, Cristina Bottino, Simona Sivori, Simona Sivori, Mariella Della Chiesa, Mariella Della Chiesa

“…Importantly, mAb-expanded adaptive NK cells did not upregulate PD-1 or other regulatory immune checkpoints that could dampen their function.ConclusionsBy this study we provide hints to improve previous expansion methods, by eliminating the use of genetically modified cells as stimulators, and obtaining effectors not expressing unwanted inhibitory receptors. …”
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Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade by Shaoyong Peng, Minshan Wu, Qian Yan, Gaopo Xu, Yumo Xie, Guannan Tang, Jinxin Lin, Zixu Yuan, Xiaoxia Liang, Ze Yuan, Jingrong Weng, Liangliang Bai, Xiaolin Wang, Huichuan Yu, Meijin Huang, Yanxin Luo, Xiaoxia Liu

“…Methods Flow cytometric, Western blotting, proteomics analysis, analysis of public database data, genetically modified cell line models, T cell coculture, crystal violetstaining, ELISA, metabonomic and clinical tumour samples were conducted to assess the role of EDEM3 in immune escape and itsmolecular mechanisms. …”
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First-in-human dose escalation trial to evaluate the clinical safety and efficacy of an anti-MAGEA1 autologous TCR-transgenic T cell therapy in relapsed and refractory solid tumors by Ignacio I Wistuba, Mamta Kalra, Linus Backert, Martin Wermke, Edwin R Parra, Jason John Luke, Apostolia M Tsimberidou, Sebastian Bunk, Mohammad B Hossain, Andrea Mayer-Mokler, Arun Satelli, Norbert Hilf, Steffen Walter, Cedrik M Britten, Van K Morris, Tobias A W Holderried, Winfried H Alsdorf, Katrin Wetzko, Borje S Andersson, Sandra Grund-Gröschke, Katrin Aslan, Anantha Marisetty, Swapna Satam, Jens Hukelmann, M Alper Kursunel, Karine Pozo, Andreas Acs, Melissa Baumeister, Claudia Wagner, Oliver Schoor, Ali S Mohamed, Delfi Krishna

“…IMA202 consists of autologous genetically modified cytotoxic CD8+ T cells expressing a T cell receptor (TCR), which is specific for a nine amino acid peptide derived from MAGEA1. …”
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Contextual Vulnerability Should Guide Fair Subject Selection in Xenotransplantation Clinical Trials by Gianna Strand

“…In a notable single-patient emergency use authorization, physician-investigators offered a genetically modified porcine heart to a living male recipient after denying him access to the waitlist for a human-donor heart, citing a history of non-compliance.…”
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