E4F1 coordinates pyruvate metabolism and the activity of the elongator complex to ensure translation fidelity during brain development by Michela Di Michele, Aurore Attina, Pierre-François Roux, Imène Tabet, Sophie Laguesse, Javier Florido, Morane Houdeville, Armelle Choquet, Betty Encislai, Giuseppe Arena, Carlo De Blasio, Olivia Wendling, François-Xavier Frenois, Laura Papon, Lucille Stuani, Maryse Fuentes, Céline Jahannault Talignani, Mélanie Rousseau, Justine Guégan, Yoan Buscail, Pierrick Dupré, Henri-Alexandre Michaud, Geneviève Rodier, Floriant Bellvert, Hanna Kulyk, Carole Ferraro Peyret, Hugo Mathieu, Pierre Close, Francesca Rapino, Cédric Chaveroux, Nelly Pirot, Lucie Rubio, Adeline Torro, Tania Sorg, Fabrice Ango, Christophe Hirtz, Vincent Compan, Elise Lebigot, Andrea Legati, Daniele Ghezzi, Laurent Nguyen, Alexandre David, Claude Sardet, Matthieu Lacroix, Laurent Le Cam

“…Here, we unravel a connection between pyruvate metabolism and the regulation of the epitranscriptome that plays an essential role during brain development. Using genetically engineered mouse model and primary neuronal cells, we identify the transcription factor E4F1 as a key coordinator of AcetylCoenzyme A (AcCoA) production by the pyruvate dehydrogenase complex (PDC) and its utilization as an essential co-factor by the Elongator complex to acetylate tRNAs at the wobble position uridine 34 (U34). …”
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Engineered T Cells for the Adoptive Therapy of B-Cell Chronic Lymphocytic Leukaemia by Philipp Koehler, Patrick Schmidt, Andreas A. Hombach, Michael Hallek, Hinrich Abken

“…Adoptive T cell therapy with genetically engineered specificity is thought to fulfil expectations, and clinical trials for the treatment of CLL are initiated. …”
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Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies by Qi Zhang, Min Li, Wenbo Hu, Xin Wang, Jinlian Hu

“…Beginning with a brief description of biological and mechanical properties of spidroin-based materials and the cellular regulatory mechanism, this review summarizes various types of spidroin-based biomaterials from genetically engineered spider silks and their prospects for specific biomedical applications (e.g., lung tissue engineering, vascularization, bone and cartilage regeneration, and peripheral nerve repair), and finally, we prospected the development direction and manufacturing technology of building more refined and customized spidroin-based protein scaffolds.…”
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CASP5 associated with PANoptosis promotes tumorigenesis and progression of clear cell renal cell carcinoma by Kangkang Yang, Yushuang Wang, Yuli Jian, Bo Wang, Hao Du, Yuqing Xia, Jianlei Bi, Meihua Guo, Zhi Li, Ning Wang

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Autophagic flux modulates tumor heterogeneity and lineage plasticity in SCLC by Yujie Hao, Mingchen Li, Wenxu Liu, Zhenyi Ma, Zhe Liu, Zhe Liu, Zhe Liu

“…Autophagy, a conserved cellular process, is implicated in many cancers, but its role in SCLC remains unclear.MethodsUsing a genetically engineered mouse model (Rb1fl/fl; Trp53fl/fl; GFP-LC3-RFP-LC3△G), we tracked autophagic flux in vivo to investigate its effects on SCLC biology. …”
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Bioartificial Liver Support by Vincent G Bain, Jose Luis Montero, Manuel de la Mata

“…The optimal source for the hepatocytes is an area of debate; however, a genetically engineered cell line may provide optimal function. …”
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Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance by Ming Luo, Sitao Zhu, Hua Dang, Qing Wen, Ruixia Niu, Jiawei Long, Zhao Wang, Yongjia Tong, Yuese Ning, Meng Yuan, Guoyong Xu

“…The X–E3TCD1 fusion protein acts as a genetically engineered degrader, selectively targeting endogenous proteins prone to condensation. …”
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Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells by Shuang Liu, Minae Takahashi, Takeshi Kiyoi, Kensuke Toyama, Masaki Mogi

“…The abilities of genetically engineered MSCs, including CRAC-overexpressing and CRAC-knockout MSCs, to differentiate into multiple mesenchymal lineages, including adipogenic, osteogenic, and chondrogenic lineages, were evaluated. …”
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Enterocyte-like differentiation defines metabolic gene signatures of CMS3 colorectal cancers and provides therapeutic vulnerability by Arezo Torang, Aleksandar B. Kirov, Veerle Lammers, Kate Cameron, Valérie M. Wouters, Rene F. Jackstadt, Tamsin R. M. Lannagan, Joan H. de Jong, Jan Koster, Owen Sansom, Jan Paul Medema

“…To identify the underlying tumorigenesis of CMS3, organoids derived from 16 genetically engineered mouse models are analyzed. Upon in vitro Cre-recombinase activation, transformation is established and transcriptional profiling reveals that distinct CMSs (CMS2-4) are modeled with different organoids. …”
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Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology by Péter Bakonyi, Nándor Nemestóthy, Katalin Bélafi-Bakó

“…The results revealed that by employing the genetically engineered E. coli (DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-type E. coli (XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L).…”
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Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction by Cameron McDonald, Alan Mackay-Sim, Denis Crane, Wayne Murrell

“…Transplanted cells were genetically engineered to express green fluorescent protein (GFP) in order to allow them to be identified after transplantation. …”
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Barriers to the adoption of multiple agricultural innovations: insights from Bt cotton, wheat seeds, herbicides and no-tillage in Pakistan by Muhammad Bilal, Tinoush Jamali Jaghdani

“…This study investigates the potential reasons that stop smallholder farming households from adopting genetically engineered seeds (Bt cotton and improved wheat seeds), herbicides resulting from cutting-edge technologies, and no-tillage farming. …”
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Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening by Channabasavaiah B. Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C. Kent Lloyd

“…In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models. The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. …”
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Engineered macrophage nanoparticles enhance microwave ablation efficacy in osteosarcoma via targeting the CD47-SIRPα Axis: A novel Biomimetic immunotherapeutic approach by Xiongfa Ji, Xin Qian, Guowen Luo, Wenjie Yang, Wenhan Huang, Zehua Lei, Jiaqi Zhou, Guoqing Zhong, Jielong Zhou, Nan Liu, Limin Ma, Mei Li, Xiangmei Liu, Shuilin Wu, Yu Zhang

“…This study developed an innovative nanotherapeutic approach by utilizing genetically engineered macrophage membranes with M1 polarization, stably overexpressing signal regulatory protein alpha (SIRPα), to encapsulate microwave-responsive nano-Prussian blue (SIRPα-M@nanoPB) nanoparticles. …”
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In Vivo Optical Molecular Imaging of Matrix Metalloproteinase Activity following Celecoxib Therapy for Colorectal Cancer by Rahul A. Sheth, Alexandra Kunin, Lars Stangenberg, Mark Sinnamon, Kenneth E. Hung, Raju Kucherlapati, Umar Mahmood

“…MMP activity was measured by an enzyme-activatable optical molecular probe. A novel genetically engineered mouse (GEM) model of colorectal cancer was also used to assess celecoxib's effect on MMP activity, which was measured by quantitative fluorescence colonoscopy. …”
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Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis by Yan Lin, Yu Dong, Yanfeng Gao, Ranran Shi, Yubing Li, Xiuman Zhou, Wenwen Liu, Guodong Li, Yuanming Qi, Yahong Wu

“…Two drug-resistant T lymphocyte epitopes, designated Rv1218c-p24 and Rv2477c-p182, were selected, and their immunogenic activities studied in vivo in genetically engineered mice. The immune activities of these two epitopes were improved with the help of complete Freund’s adjuvant (CFA). …”
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Enhancing Akt Imaging through Targeted Reporter Expression by Limin Zhang, Mahaveer S. Bhojani, Brian D. Ross, Alnawaz Rehemtulla

“…We constructed a genetically engineered hybrid bioluminescent Akt reporter (BAR) molecule that reports on Akt serine/threonine kinase activity. …”
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Cellular therapies in rheumatic and musculoskeletal diseases by Pedro Franco-Fuquen, Juana Figueroa-Aguirre, David A. Martínez, Eider F. Moreno-Cortes, Juan E. Garcia-Robledo, Fabio Vargas-Cely, Daniela A. Castro-Martínez, Mustafa Almaini, Januario E. Castro

“…The persistence of these autoreactive B cells within lymphatic organs and inflamed tissues impairs the effectiveness of B-cell-depleting monoclonal antibodies like rituximab.A promising therapeutic approach involves using T cells genetically engineered to express chimeric antigen receptors (CARs) that target specific antigens. …”
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Genetically modified chickens as bioreactors for protein-based drugs by Shujuan Meng, Aijun Miao, Sen Wu, Sen Wu, Xuguang Du, Xuguang Du, Fei Gao

“…Various species, including goats, cattle, rabbits, and poultry, have been genetically engineered to serve as bioreactors. This review delves into the analysis and comparison of different expression systems for protein drug production, highlighting the advantages and limitations of microbial, yeast, plant cell, and mammalian cell expression systems. …”
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Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis by Xiaoxiao Zhang, Jie Chu, Yuanqiang Lv, Xuan Li, Aijiao Yin, Yanhua Huang

“…., yielding the genetically engineered strains E. coli BL21-pET28a-BgaB and E. coli BL21-pET28a-araA. …”
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