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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album
Published 2022-01-01“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus
Published 2024-12-01“…Constructs encoding the recombinant antibody FM1 were designed using genetic engineering. Recombinant antibodies were produced in the CHO-K1 cell line through transient expression. …”
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Innovations in bioethanol production: A comprehensive review of feedstock generations and technology advances
Published 2025-01-01“…Batch, fed-batch, and continuous fermentation techniques are used, with advances such as immobilized cell reactors and genetic engineering improving output and efficiency. Furthermore, combining enzymatic hydrolysis with fermentation (simultaneous saccharification and fermentation) enhances the conversion of complex carbohydrates to ethanol. …”
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Species evolution: cryptic species and phenotypic noise with a particular focus on fungal systematics
Published 2025-02-01Get full text
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Functional Analysis of Durum Wheat GASA1 Protein as a Biotechnological Alternative Against Plant Fungal Pathogens and a Positive Regulator of Biotic Stress Defense
Published 2025-01-01“…This gene could therefore be a valuable target for genetic engineering to enhance wheat resistance to biotic stress.…”
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