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    Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

    Published 2022-01-01
    “…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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    Innovations in bioethanol production: A comprehensive review of feedstock generations and technology advances by Abeer Kazmi, Tahira Sultana, Amir Ali, Aneela Nijabat, Gaojie Li, Hongwei Hou

    Published 2025-01-01
    “…Batch, fed-batch, and continuous fermentation techniques are used, with advances such as immobilized cell reactors and genetic engineering improving output and efficiency. Furthermore, combining enzymatic hydrolysis with fermentation (simultaneous saccharification and fermentation) enhances the conversion of complex carbohydrates to ethanol. …”
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