Comprehensive genome-wide analysis of ARF transcription factors in orchardgrass (Dactylis glomerata): the positive regulatory role of DgARF7 in drought resistance by Miaoli Wang, Wen Liu, Guangyan Feng, Gang Nie, Zhongfu Yang, Feixiang Hao, Linkai Huang, Xinquan Zhang

“…DgARF7 also accelerated flowering time by inducing the flowering-related gene expression levels in Arabidopsis. This research provides important insights into the role of DgARF7 in orchardgrass and provides further understanding in molecular breeding.…”
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Synergistic anticancer effects of cisplatin and phenolic aglycones of the aerial part of Rumex dentatus L. in tongue squamous cell carcinoma: insights from network pharmacology and... by Amany E. Ragab, Ghada M. Al-Ashmawy, Sherin R. El Afify, Ola A. El-Feky, Amera O. Ibrahim

“…Methods The study included UPLC-ESI–MS/MS analysis, cytotoxicity assays, cell cycle analysis, apoptosis assessment, and RT-qPCR for gene expression of Bcl2, p53, and ATG7. Potential targets were identified, and mechanisms of action were examined through online databases and enrichment analyses. …”
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IL1RN and PRRX1 as a Prognostic Biomarker Correlated with Immune Infiltrates in Colorectal Cancer: Evidence from Bioinformatic Analysis by Qi Wang, Xufeng Huang, Shujing Zhou, Yuntao Ding, Huizhi Wang, Weiye Jiang, Min Xu

“…For this, we identified 704 differentially expressed genes (DEGs) by intersecting three datasets, GSE41328, GSE37364, and GSE15960 from Gene Expression Omnibus database, to maximize the accuracy of the results. …”
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Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR by Ihsène Taïhi, Ali Nassif, Tsouria Berbar, Juliane Isaac, Ariane Berdal, Bruno Gogly, Benjamin Philippe Fournier

“…RT-qPCR is a widely used technique to study gene expression and may help us to follow osteoblast differentiation of GSCs. …”
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Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome. by Brittany E Jewell, An Xu, Dandan Zhu, Mo-Fan Huang, Linchao Lu, Mo Liu, Erica L Underwood, Jun Hyoung Park, Huihui Fan, Julian A Gingold, Ruoji Zhou, Jian Tu, Zijun Huo, Ying Liu, Weidong Jin, Yi-Hung Chen, Yitian Xu, Shu-Hsia Chen, Nino Rainusso, Nathaniel K Berg, Danielle A Bazer, Christopher Vellano, Philip Jones, Holger K Eltzschig, Zhongming Zhao, Benny Abraham Kaipparettu, Ruiying Zhao, Lisa L Wang, Dung-Fang Lee

“…Transcriptome analysis of RTS osteoblasts validated decreased bone morphogenesis while revealing aberrantly upregulated mitochondrial respiratory complex I gene expression. RTS osteoblast metabolic assays demonstrated elevated mitochondrial respiratory complex I function, increased oxidative phosphorylation (OXPHOS), and increased ATP production. …”
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Food intake regulation during pregnancy and lactation in mice with reduced activity of the melanocortin system by E. N. Makarova, I. V. Romanova, N. M. Bazhan

“…In this study we measured hypothalamic AgRP and NPY mRNA levels in virgin and mated C57Bl a/a (control) and Ау/a females on days 7, 13, 18 of pregnancy, 10, 21 of lactation, and after offspring separation, AgRP immunoreactivity in virgin and lactating females, and correlated gene expression with food intake (FI). Virgin Ау/a compared to a/a females had higher FI and lower AgRP expression. …”
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Role of exercise on ncRNAs and exosomal ncRNAs in preventing neurodegenerative diseases: a narrative review by Shangwu Liu, Runhong Zhang, Jamal Hallajzadeh

“…Non-coding RNAs (ncRNAs) and exosomal ncRNAs associated with vesicles have been recognized as influencers of gene expression and cell signaling, potentially contributing to the positive impact of physical activity on neurodegenerative conditions. …”
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Hairpin in a haystack: In silico identification and characterization of plant-conserved microRNA in Rafflesiaceae by Wicaksono Adhityo, Meitha Karlia, Wan Kiew-Lian, Mat Isa Mohd Noor, Parikesit Arli Aditya, Molina Jeanmaire

“…Derived from secondary structures or hairpins, miRNAs are small regulators of gene expression. We found that some plant-conserved miRNA still exists in Rafflesiaceae. …”
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The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats by Mojdeh Khajehlandi, Lotfali Bolboli

“…One-way analysis of variance with LSD’s post-hoc test with a significant level ofP≤0.05 was used to analyze data.Results: Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (Casp-8) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, whileimproving inflammatory markers and the lipid profile (P<0.05).Conclusion: Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile byquercetin injection may be a promising approach for the development of new antidiabetic medications. …”
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The Prognostic Significance of TRs in Hepatocellular Carcinoma: Insights from TCGA and GEO Databases by Hao Zhou, Weijie Wang, Ruopeng Liang, Rongtao Zhu, Jiahui Cao, Chenguang Sun, Yuling Sun

“…Expression levels of TRs in HCC and immune single cells were assessed using datasets from the Gene Expression Omnibus (GEO) and TCGA, analyzed with R software. …”
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Development of a 3-MicroRNA Signature and Nomogram for Predicting the Survival of Patients with Uveal Melanoma Based on TCGA and GEO Databases by Jun Zuo, Hongquan Ye, Jing Tang, Jianqun Lu, Qi Wan

“…Then, miRNAs data of GSE84976 and GSE68828 were downloaded from Gene Expression Omnibus (GEO) database for outside verification. …”
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Bioinformatics Analysis of Genes and Mechanisms in Postherpetic Neuralgia by Yong Qiu, Meng-Lei Hao, Xu-Tao Cheng, Zhen Hua

“…We analyzed the GSE64345 dataset, which includes gene expression from the ipsilateral dorsal root ganglia (DRG) of PHN model rats. …”
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A telomere-related signature for predicting prognosis and assessing immune microenvironment in osteosarcoma by Shihao Li, Lina Zhang, Haiyang Zhang

“…Thus, we established a telomere-related signature in osteosarcoma to comprehensively evaluate the pathogenic roles of telomeres in this disease.MethodsThe data on osteosarcoma were collected from the TARGET and Gene Expression Omnibus databases. First, we constructed a telomere-related signature using univariate and LASSO Cox regression analyses. …”
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A Robust Metatranscriptomic Technology for Population-Scale Studies of Diet, Gut Microbiome, and Human Health by Andrew Hatch, James Horne, Ryan Toma, Brittany L. Twibell, Kalie M. Somerville, Benjamin Pelle, Kinga P. Canfield, Matvey Genkin, Guruduth Banavar, Ally Perlina, Helen Messier, Niels Klitgord, Momchilo Vuyisich

“…Viomega includes sample collection, ambient temperature sample preservation, total RNA extraction, physical removal of ribosomal RNAs (rRNAs), preparation of directional Illumina libraries, Illumina sequencing, taxonomic classification based on a database of >110,000 microbial genomes, and quantitative microbial gene expression analysis using a database of ~100 million microbial genes. …”
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Tire rubber-derived contaminants 6PPD and 6PPD-quinone reduce attachment and outgrowth of trophoblast spheroids onto endometrial epithelial cells by Jong Geol Lee, Seon Min Lee, Moonjung Hyun, Jeong Doo Heo

“…A similar result was observed in 1 μM 6PPDQ-exposed groups. Gene expression profiling of 6PPD- and 6PPDQ-exposed endometrial epithelial cells revealed that both 6PPD and 6PPDQ differentially regulated a panel of transcript markers toward overall downregulation of receptivity and invasion. …”
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The Efficacy of Fecal Microbiota Transplantation in Experimental Autoimmune Encephalomyelitis: Transcriptome and Gut Microbiota Profiling by Sanwang Wang, Hongliang Chen, Xin Wen, Jingjing Mu, Mingyue Sun, Xiaowen Song, Bin Liu, Jinbo Chen, Xueli Fan

“…Altogether, FMT may selectively regulate gene expression to improve inflammation and maintain the stability of the intestinal environment in a gut microbiota-dependent manner.…”
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An Open‐Label Phase II Trial of Pembrolizumab, an Immune Checkpoint Inhibitor Alone or in Combination With Oral Azacitidine as Second‐Line Therapy for Advanced Head and Neck Squamo... by Vinod K. Ramani, Srimonta Gayen, Radheshyam Naik

“…Epigenetics are heritable reversible changes in gene expression that occur without any changes in the nuclear DNA sequence or DNA copy number. …”
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Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory by Xiaomei Zheng, Yuting Guo, Meiling Chen, Yudan Lu, Yimou Du, Yu Lei, Ping Zheng, Jibin Sun

“…A wide dynamic range of promoters, particularly strong promoters, are required for fine-tuning the regulation of gene expression to balance metabolic flux and achieve the high yields of desired products. …”
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Machine learning-random forest model was used to construct gene signature associated with cuproptosis to predict the prognosis of gastric cancer by Xiaolong Liu, Pengxian Tao, He Su, Yulan Li

“…Transcriptome and clinical data of patients with GC were collected from The Cancer Genome Atlas and Gene Expression Omnibus datasets. Single sample gene set enrichment analysis (GSEA) and the randomized forest method were used to establish the prognostic model. …”
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Acidovorax temperans skews neutrophil maturation and polarizes Th17 cells to promote lung adenocarcinoma development by Joshua K. Stone, Natalia von Muhlinen, Chenran Zhang, Ana I. Robles, Amy L. Flis, Eleazar Vega-Valle, Akihiko Miyanaga, Masaru Matsumoto, K. Leigh Greathouse, Tomer Cooks, Giorgio Trinchieri, Curtis C. Harris

“…These T17 cells shared a common gene expression program predictive of poor survival in human LUAD. …”
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