Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…Cas9-BD and similarly modified dCas9-BD have been successfully employed for simultaneous biosynthetic gene cluster (BGC) refactoring, multiple BGC deletions, or multiplexed gene expression modulations in Streptomyces. We also demonstrate improved secondary metabolite production using multiplexed genome editing with multiple single guide RNA libraries in several Streptomyces strains. …”
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Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats by Pablo Muñoz, Carolina Estay, Paula Díaz, Claudio Elgueta, Álvaro O. Ardiles, Pablo A. Lizana

“…Although the importance of DNA methylation-dependent gene expression to neuronal plasticity is well established, the dynamics of methylation and demethylation during the induction and expression of synaptic plasticity have not been explored. …”
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De Novo Transcriptome Sequencing of Olea europaea L. to Identify Genes Involved in the Development of the Pollen Tube by Domenico Iaria, Adriana Chiappetta, Innocenzo Muzzalupo

“…In order to determine compatibility relationships, using next-generation sequencing techniques and a de novo transcriptome assembly strategy, we show that pollen tubes from different olive plants, grown in vitro in a medium containing its own pistil and in combination pollen/pistil from self-sterile and self-fertile cultivars, have a distinct gene expression profile and many of the differentially expressed sequences between the samples fall within gene families involved in the development of the pollen tube, such as lipase, carboxylesterase, pectinesterase, pectin methylesterase, and callose synthase. …”
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Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis by Zijiang Yang, Ting Zhang, Xuanlin Zhu, Xiaobiao Zhang

“…We further unraveled the biological functions and clinical implications of cold-inducible RNA-binding protein (CIRBP) in the context of glioblastoma by using a multifaceted approach, encompassing gene expression profiling, survival analysis, and functional assays to elucidate its role in glioblastoma cell mortality and its potential influence on patient prognosis. …”
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Supplementation with serine-enriched non-essential amino acids from minimum essential medium promotes blastocyst development of in vitro-fertilized bovine embryos by Nobuhiko ITAMI, Yuji HIRAO

“…Analysis of endogenous serine synthases gene expression in oocytes and embryos revealed that phosphoglycerate dehydrogenase, the rate-limiting enzyme in the serine synthesis pathway, is expressed at the morula stage and beyond. …”
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Genome-wide DNA methylation and transcriptome sequencing analyses of lens tissue in an age-related mouse cataract model. by Yuzhu Hu, Dongmei Su, Yue Zhang, Yanjiang Fu, Sijia Li, Xiaoya Chen, Xiao Zhang, Shunfei Zheng, Xu Ma, Shanshan Hu

“…In conclusion, we confirmed the genome-wide DNA methylation pattern and gene expression profile of ARC based on the mouse cataract model with aged mice. …”
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Expression Characterization of Six Genes Possibly Involved in Gonad Development for Stellate Sturgeon Individuals (Acipenser stellatus, Pallas 1771) by Alexandru Burcea, Gina-Oana Popa, Iulia Elena Florescu (Gune), Marilena Maereanu, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache

“…For the purpose of understanding the sexual development of 4-year-old stellate sturgeon (Acipenser stellatus) individuals, we have selected six genes (foxl2, cyp17a1, ar, dmrt1, sox9, and star). We analysed the gene expression of the selected genes for gonads, anal fin, liver, body kidney, and white muscle. …”
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BMP9 induces postnatal zonal stratification of immature articular cartilage through reconfiguration of the existing collagen framework by Miles Anderson-Watters, Ilyas M. Khan

“…We found that stromelysin-1 (metalloproteinase-3, MMP3) gene expression was consistently upregulated, whilst other MMP transcript levels were unchanged or reduced. …”
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The Assessment of CDX1, IHH, SHH, GATA4, FOXA2, FOXF1 in Congenital Intra-Abdominal Adhesions by Helēna Freijere Pope, Māra Pilmane, Anna Junga, Aigars Pētersons

“…This research examines the expression of Caudal Type Homeobox 1 (CDX1), Indian Hedgehog (IHH), Sonic Hedgehog (SHH), GATA Binding Protein 4 (GATA4), Forkhead Box A2 (FOXA2) and Forkhead Box F1 (FOXF1) gene expression in human abdominal congenital adhesion fibroblast and endothelium cells by chromogenic in situ hybridization, with the aim of elucidating their potential association with the etiology of congenital intra-abdominal adhesion band development. …”
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Aplication of Gene Transfer in Aquaculture by . Alimuddin, G. Yoshizaki, O. Carman, K. Sumantadinata

“…Key words: Gene transfer, gene expression, biotechnology,  aquaculture   ABSTRAK Saat ini, keamanan pangan telah menjadi isu hangat di masyarakat baik di dalam maupun di luar negeri. …”
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Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance by Mojdeh Akbarzadeh Lelekami, Mohammad Hadi Pahlevani, Khalil Zaynali Nezhad, Keyvan Mahdavi Mashaki

“…Abstract Environmental stresses, particularly salinity, pose significant challenges to global crop production, notably impacting the growth and yield of rice. Integrating gene expression and metabolomics data offers valuable insights into the molecular mechanisms driving salt tolerance in plants. …”
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Unveiling cell-type-specific microRNA networks through alternative polyadenylation in glioblastoma by Mert Cihan, Greta Schmauck, Maximilian Sprang, Miguel A. Andrade-Navarro

“…A significant finding was the disconnection between differential APA events and gene expression alterations, indicating that APA operates as an independent regulatory mechanism. …”
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A Comparative Genomic Study in Schizophrenic and in Bipolar Disorder Patients, Based on Microarray Expression Profiling Meta-Analysis by Marianthi Logotheti, Olga Papadodima, Nikolaos Venizelos, Aristotelis Chatziioannou, Fragiskos Kolisis

“…In another study we used samples from postmortem orbitofrontal cortex of bipolar subjects while the final study was performed based on raw data from a gene expression profiling dataset in the postmortem superior temporal cortex of schizophrenics. …”
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Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy by Yicun Wang, Yuxuan Wang, Di Zhang, Hao Zhang, Wei Wang, Xiaopeng Hu

“…BK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We collected gene expression profiles of 169 kidney biopsies taken from BKVN, rejection, and stable functioning allografts, based on single sample gene set enrichment analysis and random forest algorithm, and three hallmark activities associated with DNA damage and proliferation were found to be relatively specific in BKVN. …”
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New insights into N6-methyladenosine in hepatocellular carcinoma immunotherapy by Mengran Li, Mengran Li, Hu Tian, Yanshuang Zhuang, Zili Zhang, Zili Zhang

“…This modification is crucial for controlling important functions that are vital for gene expression, including mRNA splicing, stability, and translation. …”
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Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells... by Helia Ghafaripour, Masoud Homayouni Tabrizi, Ehsan Karimi, Niloofar Barati Naeeni

“…Moreover, lawsone‐loaded nanoparticle upregulated BAX gene expression, while downregulated BCL2expression, suggesting the activation of apoptotic pathway. …”
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The impact of KRAS mutations on the tumour microenvironment and treatment response in non-small cell lung cancer by Guomin Gu, Chunling Liu, Yan Yang, Yan Zhao, Xiaodan Zhu, Gang Sun

“…Mutations in the KRAS gene in non-small cell lung cancer (NSCLC) are common drivers. Gene expression and mutation data of NSCLC were collected from the TCGA dataset. …”
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Combined Effects of 2-Methoxyestradiol (Hypoxia-Inducible Factor 1α Inhibitor) and Dasatinib (A Second-Generation Tyrosine Kinase Inhibitor) on Chronic Myelocytic Leukemia Cells by Yuexian Zhang, Heng Chen, Yunfeng Shen, Xin Zhou

“…Once the concentration of 2-ME2 exceeded 0.5 μM, downregulated C-myc gene expression could exert roles in anti-CML cell proliferation and inducing apoptosis. …”
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Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells by Hiroyuki Kamao, Atsushi Miki, Junichi Kiryu

“…RPE function was evaluated by measuring the secretion of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) and RPE-specific gene expression. Results. Rates of cell damage in hfRPE and both hiPS-RPE were increased by rtPA supplementation (2000 and 4000 μg/ml) for 1 hour, whereas ARPE19 cell damage was increased by supplementation with rtPA at concentrations higher than 50 μg/ml. …”
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Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses by Tsheten Sherpa, Tsheten Sherpa, Nrisingha Dey

“…In this work, we attempted to increase the constitutive promoter library by developing efficient synthetic promoters suitable for high-level gene expression. To do that, we selected three strong pararetroviral-based promoters from Mirabilis mosaic virus (MMV), Figwort mosaic virus (FMV), and Horseradish latent virus (HRLV) and rationally designed and combined their promoter elements. …”
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