Influence of nano-polystyrene on cyfluthrin toxicity in honeybee Apis cerana cerana Fabricius by Yunfei Xue, Xueyang Gong, Linfu Yang, Dandan Zhi, Qingxin Meng, Yulong Guo, Kun Dong, Yakai Tian

“…Regarding immune gene expression, exposure to a single toxin or both toxins significantly down-regulated the abaecin gene, but only exposure to nanoPS significantly decreased apidaecin expression. …”
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ANO7 expression in the prostate modulates mitochondrial function and lipid metabolism by Christoffer Löf, Nasrin Sultana, Neha Goel, Samuel Heron, Gudrun Wahlström, Andrew House, Minna Holopainen, Reijo Käkelä, Johanna Schleutker

“…Methods We performed RNA-sequencing (RNA-seq) on RWPE1 cells engineered to express ANO7 protein, alongside the analysis of a single-cell RNA-sequencing (scRNA-seq) dataset and RNA-seq from prostate tissues. Differential gene expression analysis and gene set enrichment analysis (GSEA) were conducted to identify key pathways. …”
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E3 ubiquitin ligase SYVN1 as a promising therapeutic target for diverse human diseases by Li Zhu, Yong-Ping Liu, Yuan-Wang, Bo-Xuan Sun, Yu-Ting Huang, Ji-Kai Zhao, Jian-Feng Liu, Li-Ming Yu, Hui-Shan Wang

“…SYVN1, an E3 ubiquitin ligase, is characterized by its significant functions in regulating various biological processes, including molecular mechanisms related to gene expression, signaling pathways, and cell death, among others. …”
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Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats by Hancai Jiang, Xiaoxian Xu, Shuwan Wang, Xinhui Song, Ling Li, Qingyou Liu, Kuiqing Cui, Deshun Shi, Jian Wang, Hui Li, Jieping Huang, Zhipeng Li

“…Gene expression and post-transcriptional regulation are key mechanisms affecting lactation performance in dairy animals. …”
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A novel early stage drip irrigation system cost estimation model based on management and environmental variables by Masoud Pourgholam-Amiji, Khaled Ahmadaali, Abdolmajid Liaghat

“…Then, different machine learning models such as Multivariate Linear Regression, Support Vector Regression, Artificial Neural Networks, Gene Expression Programming, Genetic Algorithms, Deep Learning, and Decision Trees, were used to estimate the costs of each of the of the aforementioned sections. …”
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ITGA4 as a potential prognostic and immunotherapeutic biomarker in human cancer and its clinical significance in gastric cancer: an integrated analysis and validation by Jiaxing Zhang, Jiaxing Zhang, Gang Wang, Jie Liu, Futian Tang, Song Wang, Yumin Li, Yumin Li

“…ITGA4 positively correlated with immune cell infiltration, immune regulatory genes, chemokines, and might reduce microsatellite instability (MSI) and tumor mutation burden (TMB) by promoting MMR gene expression. It could also predict immunotherapy efficacy and chemotherapy sensitivity. …”
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FBR2 modulates ferroptosis via the SIRT3/p53 pathway to ameliorate pulmonary fibrosis by Yu Cheng, Yang Jiao, Wan Wei, Mengjia Kou, Yaodong Cai, Yang Li, Hao Li, Tonghua Liu

“…Assessments encompassed histopathology, ELISA for cytokine detection, IHC and Western blot for protein expression analysis, and qRT-PCR for gene expression quantification. Transmission electron microscopy (TEM) was used to observe mitochondrial morphology. …”
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Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC by Yulin Zhang, Yixu Wang, Jilin Peng, Kun Zhao, Ling Li, Yuan Zhang, Ziyu Zhai, Sijie Yuan, Shichao Li, Shichao Li, Shichao Li, Fanglei Ye, Le Wang

“…Further experiments were performed in patient samples, stable cell lines, and a murine xenograft tumor model.ResultsA reliable survival risk model of m6A was constructed based on the TCGA database. Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. …”
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High-altitude hypoxia aggravated neurological deficits in mice induced by traumatic brain injury via BACH1 mediating astrocytic ferroptosis by Peng Zou, Tianjing Li, Zixuan Cao, Erwan Yang, Mingdong Bao, Haofuzi Zhang, Zhuoyuan Zhang, Dan Liu, Min Zhang, Xiangyu Gao, Junmiao Ge, Xiaofan Jiang, Zhicheng Tian, Peng Luo

“…Single-nuclear RNA sequencing was subsequently employed to further reveal differential gene expression profiles in high-altitude TBI. We found a significant increase in ferroptosis of astrocytes in cases of high-altitude TBI compared to those at low-altitude TBI. …”
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To construct and validate a risk score model of angiogenesis-related genes to predict the prognosis of hepatocellular carcinoma by Duangui Gao, Yuan Lu, Tianpeng Jiang, Qinghong Duan, Zhi Huang

“…GSVA analysis was used to assess the variation of gene sets between groups, and ClBERSOFT, ESTIMATE, and TIMER databases were used to analyze the immune infiltration in the single-cell level analysis of gene expression differences between cells. Finally, in the three pairs of HCC tissues and tissue adjacent to carcinoma by real-time fluorescent quantitative PCR (qRT_PCR) and western blotting (WB) to evaluate angiogenesis-related genes (ATP2A3 AEBP1 PNMA1, PLAT) expression level in HCC, and AEBP1 was knocked out in HCCLM3 cells, which is to study AEBP1 biological function in HCC. …”
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The m6A modification regulates the composition of myofiber types in chicken skeletal muscle by Yanju Shan, Yifan Liu, Ming Zhang, Lichuan Pang, Gaige Ji, Xiaojun Ju, Yunjie Tu, Jingting Shu

“…Further analysis revealed that the m6A methylation was positively correlated with gene expression homeostasis. It is exciting we found that the expression level of ALKBH5 mRNA and protein, was closely related to the composition of myofiber types. …”
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OLIG2 mediates a rare targetable stem cell fate transition in sonic hedgehog medulloblastoma by Kinjal Desai, Siyi Wanggou, Erika Luis, Heather Whetstone, Chunying Yu, Robert J. Vanner, Hayden J. Selvadurai, Lilian Lee, Jinchu Vijay, Julia E. Jaramillo, Jerry Fan, Paul Guilhamon, Michelle Kushida, Xuejun Li, Gregory Stein, Santosh Kesari, Benjamin D. Simons, Xi Huang, Peter B. Dirks

“…Previously, we demonstrated that the growth of the paediatric brain malignancy, sonic hedgehog subgroup medulloblastoma, is rooted in a dysregulated developmental hierarchy, the apex of which is defined by characteristically quiescent SOX2+ stem-like cells. Integrating gene expression and chromatin accessibility patterns in distinct cellular compartments, we identify the transcription factor Olig2 as regulating the stem cell fate transition from quiescence to activation, driving the generation of downstream neoplastic progenitors. …”
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Evaluation of exo-long noncoding RNA MALAT1 in OSCC in comparison to dysplastic and normal: A cross-sectional study by Ramya Sekar, Selvaraj Jayaraman, VishnuPriya Veeraraghavan, Kalaiselvi Krishnamoorthy, Shubhra Chauhan Aramanai, Sathan Raj Natarajan

“…RNA concentration was estimated, real-time polymerase chain reaction (qPCR) was used for quantitative analysis. Gene expression levels of MALAT1, PI3K, AKT1, and PTEN were analysed and compared across groups using one way ANOVA and Post-hoc Tukey analysis was performed for pairwise comparisons to assess correlations between MALAT1 expression and PI3K/AKT pathway components. …”
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Lactate Dehydrogenase-A-Forming LDH5 Promotes Breast Cancer Progression by Fang T, Hu L, Chen T, Li F, Yang L, Liang B, Wang W, Zeng F

“…We aimed to explore the roles of LDHA and LDH5 isoenzyme activity in BC, which provides a new insight into LDHA for the treatment of BC.Methods: The expression of LDHA in BC and its relationship with clinicopathological features were obtained from various databases including The Cancer Genome Atlas (TCGA), Human Protein Atlas (HPA), Breast Cancer-Gene Expression Miner (bc-GenExMiner), TNMplot, UALCAN. …”
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Comparative transcriptomics reveal stage-dependent parasitic adaptations in the myxozoan Sphaerospora molnari by Monika M. Wiśniewska, Jiří Kyslík, Gema Alama-Bermejo, Alena Lövy, Martin Kolísko, Astrid S. Holzer, Anush Kosakyan

“…Results We used parasite isolation protocols and comparative transcriptomics to study early proliferative and spore-forming stages of S. molnari, unveiling variation in gene expression between each stage. We discovered several apparent innovations in the S. molnari transcriptome, including proteins that are likely to function in the uptake of previously unknown key nutrients, immune evasion factors that may contribute to long-term survival in hosts, and proteins that likely improve adhesion to host cells that may have arisen from horizontal gene transfer. …”
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Molecular characterization of pregnancy-associated breast cancer and insights on timing from GEICAM-EMBARCAM study by Regina Peña-Enríquez, Begoña Bermejo, Marina Pollán, Alejandra Díaz-Chacón, Yolanda Jerez Gilarranz, José J Ponce Lorenzo, Antonio Fernández Aramburo, Blanca Cantos Sánchez de Ibargüen, Ana Santaballa Bertrán, Elena Galve-Calvo, Álvaro Jiménez-Arranz, Yolanda Fernández, María Eva Pérez, Susana De La Cruz, Antonio Anton-Torres, Fernando Moreno, María Jesús Vidal-Losada, María Helena López-Ceballos, Isabel Blancas, María José Echarri, Raúl Rincón, Rosalía Caballero, Ángel Guerrero-Zotano, Silvia Guil-Luna, Juan de la Haba-Rodríguez

“…This study analyzed the clinicopathological characteristics and gene expression profile of 33 PABC and 26 non-PABC patients using the nCounter BC360 Panel (NanoString). …”
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Characterization of the genomic landscape of canine diffuse large B-cell lymphoma reveals recurrent H3K27M mutations linked to progression-free survival by Anna Darlene van der Heiden, Raphaela Pensch, Sophie Agger, Heather L. Gardner, William Hendricks, Victoria Zismann, Shukmei Wong, Natalia Briones, Bryce Turner, Karin Forsberg-Nilsson, Cheryl London, Kerstin Lindblad-Toh, Maja Louise Arendt

“…In particular, we identified hotspot mutations in two histone genes, H3C8, and LOC119877878, resulting in H3K27M alterations predicted to dysregulate gene expression. Finally, a survival analysis revealed that H3K27M mutations in H3C8 were associated with increased hazard ratios for progression-free survival. …”
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Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer’s Disease by Zhou H, Peng Y, Huo X, Li B, Liu H, Wang J, Zhang G

“…However, the cellular and molecular links between ferroptosis, immune inflammation, and the brain-peripheral blood axis in Alzheimer’s disease (AD) remain unclear.Methods: We integrated bulk RNA-seq data from AD brain tissue and peripheral blood and refined the screening of AD candidate genes through differential gene expression analysis, weighted gene co-expression network analysis (WGCNA), and other approaches. …”
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Mycochemistry, antioxidant, anticancer activity, and molecular docking of compounds of F12 of ethyl acetate extract of Astraeus asiaticus with BcL2 and Caspase 3 by Swapan Kumar Ghosh, Koushik Pandey, Madhuparna Ghosh, Pradip Kumar Sur

“…The mechanism of anticancer effect of F12 (AAEAE) on cancer cell lines included induction of apoptosis, LDH leakage, and up regulation of gene expression levels of Caspase 3, Caspase 9, P53, and down regulation of BcL2 of all three cell lines. …”
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Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher p... by Joseph P. Gulizia, Zubair Khalid, Maria T. Terra-Long, Jose I. Vargas, Jose R. Hernandez, Wilmer J. Pacheco, James T. Krehling, Kenneth S. Macklin, William A. Dozier, III, Klint W. McCafferty, Ruediger Hauck

“…Jejunum wall and cecal content samples were collected on d 28 and 42 to assess jejunum villus height (VH), crypt depth, tight-junction and mucin gene expression, cecal microbiome diversity, and predicted bacterial metabolic pathways. …”
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