MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair by Jiandong Zhang, Lei Ma, Limin He, Quanxiao Xu, Yan Ding, Lidong Wang

“…Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively. …”
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Metformin reverts aortic calcifications and elastin loss induced by an experimental metabolic syndrome by Lucas Streckwall, Nancy Martini, Claudia Sedlinsky, León Schurman, María Virginia Gangoiti, Antonio Desmond McCarthy

“…Aortas were dissected for histomorphometric and immunohistochemical analysis, ex vivo calcification studies and isolation of VSMCs to measure their alkaline phosphatase activity (ALP), collagen production, extracellular mineralization, gene expression of RUNX2 and receptor for advanced glycation end-products (AGEs) (RAGE), and elastic fiber production. …”
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Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis by Danial Hashemi Karoii, Hossein Azizi, Maryam Darvari, Ali Qorbanee, Dawan Jamal Hawezy

“…Conclusion Our analysis, leveraging single-cell data, substantiated our findings, demonstrating significant alterations in gene expression patterns.…”
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Identification of CD8+ T Cell-Related Genes: Correlations with Immune Phenotypes and Outcomes of Liver Cancer by Qi Pan, Ying Cheng, Donghua Cheng

“…The “estimate” package and the CIBERSORT algorithm were used to evaluate tumor purity and the proportion of CD8+ T lymphocytes in the liver cancer cohort. We used the gene expression matrix of liver cancer and the relative proportion of CD8+ T lymphocytes as input files and performed WGCNA based on this analysis. …”
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Reducing the dietary starch:protein ratios in low-protein diets enhanced the growth performance of goslings from 1 to 28 days of age by Xucheng Zheng, Qian Lu, Chunhui Huang, Usman Nazir, Zhi Yang, Haiming Yang, Zhiyue Wang

“…Moreover, SGLT1 and GLUT2 genes expression were generally down-regulated, and SLC7A5 gene expression was up-regulated in reduced S: P ratio groups. …”
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Impact of copper stress in the intestinal barriers and gut microbiota of Chinese stripe-necked turtle (Mauremys sinensis) by Zeeshan Ali, Ijaz Khan, Muhammad Shahid Iqbal, Haitao Shi, Li Ding, Meiling Hong

“…This study investigates the effects of copper on the intestinal health of Chinese stripe-necked turtle, focusing on histomorphology, mucosal barrier function, gene expression, and gut microbiota. Copper stress caused intestinal damage, characterized by shortened villi, inflammatory cell infiltration, and reduced epithelial layer thickness, as well as decreased acidic mucins, increased villi edema and inflammation. …”
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Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications by Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, Garrett Wong, Isha Dey, Spencer Holmes, Abigail Dockey, Lindsay Bailey-Steinitz, Lina Zheng, Weizhong Li, Vivek Chandra, Jakhan Nguyen, Jason Sharp, Erik Willems, Mark Kennedy, Matthew R. Dallas, David Kuninger

“…Abstract Tumoroids, sometimes referred to as cancer organoids, are patient-derived cancer cells grown as 3D, self-organized multicellular structures that maintain key characteristics (e.g., genotype, gene expression levels) of the tumor from which they originated. …”
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CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway by Qianguang Han, Bin Ni, Wei Bao, Junqi Zhang, Ming Zheng, Jinxu Miu, Zijie Wang, Jingwen Yuan, Jun Tao, Zhijian Han, Min Gu, Xiaobing Ju, Ruoyun Tan

“…However, the mechanism by which ferroptosis contributes to the onset and progression of CAD remains unclear.MethodsThis study analyzed transcriptome data from renal transplant biopsy samples in the Gene Expression Omnibus (GEO), through clinical samples, animal models, and cell experiments, this study investigated the mechanism by which Caveolin-1 (CAV1) promotes CAD through the regulation of the ferroptosis pathway.ResultsThe elevated levels of CAV1 were found to positively correlate with CAD incidence. …”
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Identification of a distinctive immunogenomic gene signature in stage-matched colorectal cancer by Pankaj Ahluwalia, Ashis K. Mondal, Ashutosh Vashisht, Harmanpreet Singh, Ahmet Alptekin, Kalyani Ballur, Nivin Omar, Meenakshi Ahluwalia, Kimya Jones, Amanda Barrett, Vamsi Kota, Ravindra Kolhe

“…Orthogonal analyses included single-cell profiling, differential gene expression, immune cell deconvolution, neoantigen prediction, and biological pathway assessment. …”
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Mendelian Randomization Reveals Potential Causal Relationships Between DNA Damage Repair-Related Genes and Inflammatory Bowel Disease by Zhihao Qi, Quan Li, Shuhua Yang, Chun Fu, Burong Hu

“…We found that DNA methylation of <i>Erbb2</i> and <i>Gpx1</i> significantly impacted their gene expression levels offering insights into the potential regulatory mechanisms of risk variants on IBD. …”
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Study on the effects of Mogroside V in inhibiting NLRP3-mediated granulosa cell pyroptosis and insulin resistance to improve PCOS by Wenqin Yang, Yujie Ma, Yafei Wu, Xiaocan Lei, Jing Zhang, Meixiang Li

“…Transcriptome analysis identified significant gene expression changes between groups, particularly in pathways related to NLRP3 inflammation and insulin metabolism. …”
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Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense<... by Chang Yang, Bo Liu, Liangkun Pan, Dong Xia, Cunxin Sun, Xiaochuan Zheng, Peng Chen, He Hu, Qunlan Zhou

“…The results showed that the low-fishmeal diet induced the following: (1) the inhibition of growth performance and survival of prawns; (2) an increase in triglyceride content in the hepatopancreas and hemolymph and downregulation of <i>carnitine palmitoyl transferase 1</i> (<i>cpt1</i>) gene expression; (3) a reduction in antioxidant enzymes’ activities and their genes expression levels and an increase malondialdehyde (MDA) content; and (4) an increase in the abundance of the conditional pathogen <i>Pseudomonas</i> in the gut. …”
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Influence of nano-polystyrene on cyfluthrin toxicity in honeybee Apis cerana cerana Fabricius by Yunfei Xue, Xueyang Gong, Linfu Yang, Dandan Zhi, Qingxin Meng, Yulong Guo, Kun Dong, Yakai Tian

“…Regarding immune gene expression, exposure to a single toxin or both toxins significantly down-regulated the abaecin gene, but only exposure to nanoPS significantly decreased apidaecin expression. …”
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Evaluating H2BC9 as a potential diagnostic and prognostic biomarker in head and neck squamous cell carcinoma by Lanhua Wu, Liang Li, Mingjing Zhu, Ziyan Zhou, Xuejin Su, Yueming Jiang, Min Kang, Li Jiang

“…H2B clustered histone 9 (H2BC9), a member of the H2B family, plays critical roles in gene expression regulation, chromosome structure, DNA repair stability, and cell cycle regulation. …”
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ANO7 expression in the prostate modulates mitochondrial function and lipid metabolism by Christoffer Löf, Nasrin Sultana, Neha Goel, Samuel Heron, Gudrun Wahlström, Andrew House, Minna Holopainen, Reijo Käkelä, Johanna Schleutker

“…Methods We performed RNA-sequencing (RNA-seq) on RWPE1 cells engineered to express ANO7 protein, alongside the analysis of a single-cell RNA-sequencing (scRNA-seq) dataset and RNA-seq from prostate tissues. Differential gene expression analysis and gene set enrichment analysis (GSEA) were conducted to identify key pathways. …”
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Transcriptome analysis of ovarian cancer uncovers association between tumor-related inflammation/immunity and patient outcome by Jingfang Wang, Wenrui Zhu, Xia Li, Yuanyuan Wu, Wenhui Ma, Yangzhou Wang, Weihong Zhao, Fang Wei, Wenhao Wang

“…The immune infiltration microenvironment, clinicopathological features, and survival rates differed significantly between two inflammatory gene expression patterns identified using cluster and immune microenvironment analyses. …”
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Cathepsin G promotes arteriovenous fistula maturation by positively regulating the MMP2/MMP9 pathway by Lemei Hu, Changqing Zheng, Ying Kong, Zhiqing Luo, Fengzhang Huang, Zhigang Zhu, Quhuan Li, Ming Liang

“…A more comprehensive understanding and study of the mechanisms of AVF maturation is urgently needed.Methods and results In this study, we downloaded the publicly available datasets (GSE119296 and GSE220796) from the Gene Expression Omnibus (GEO) and merged them for subsequent analysis. …”
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Knockdown ATG5 gene by rAAV9 alleviates doxorubicin-induced cardiac toxicity by inhibiting GATA4 autophagic degradation by Ai-Li Xu, Zheng Shen, Shi-Hao Wang, Hai-Yun Luan, Yong Xu, Ze-Chun Kang, Zi-Qi Liao, Jie Liu, Xiao-Lei Duan, Wei-Hua Bian, Hui Sun, Xin Xie

“…DOX treatment upregulated autophagy-related gene expression but inhibited autophagic flux in vitro and in vivo. …”
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SNORA37/CMTR1/ELAVL1 feedback loop drives gastric cancer progression via facilitating CD44 alternative splicing by Banghe Bao, Minxiu Tian, Xiaojing Wang, Chunhui Yang, Jiaying Qu, Shunchen Zhou, Yang Cheng, Qiangsong Tong, Liduan Zheng

“…Alternative splicing and gene expression was observed by real-time quantitative RT-PCR and western blot assays. …”
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Short-Term Activation of Peroxisome Proliferator-Activated Receptors α and γ Induces Tissue-Specific Effects on Lipid Metabolism and Fatty Acid Composition in Male Wistar Rats by Elin Strand, Vegard Lysne, Mari Lausund Grinna, Pavol Bohov, Asbjørn Svardal, Ottar Nygård, Rolf K. Berge, Bodil Bjørndal

“…Lipids and FA methyl esters were measured in plasma, liver, and heart and gene expression was measured in liver and adipose tissue, while enzyme activities were measured in liver. …”
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