Blood-Brain Barrier Abnormalities Caused by HIV-1 gp120: Mechanistic and Therapeutic Implications by Jean-Pierre Louboutin, David S. Strayer

“…Thus, (1) HIV-envelope gp120 disrupts the BBB; (2) this occurs via lesions in brain microvessels, MMP activation and degradation of vascular basement membrane and vascular tight junctions; (3) NMDAR-1 activation plays a role in this BBB injury; and (4) antioxidant gene delivery as well as NMDAR-1 antagonists may protect the BBB.…”
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Recent achievement of graphene in biomedicine: Advancements by integrated microfluidics system and conventional techniques by Mohammad Aminul Islam, Aslam Hossain, Nayem Hossain, Md Mir Shakib Ahmed, Safiul Islam, A.M.A. Henaish, A.V. Soldatov, Mohammad Asaduzzaman Chowdhury

“…While many studies have focused on individual applications of graphene, this review uniquely present a holistic view of its potential across various biomedical fields, including drug delivery, gene delivery, tissue engineering, and photothermal treatment, detection, sensor with respect to conventional and microfluidics techniques. …”
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Immunocompatibility of Bacteriophages as Nanomedicines by Tranum Kaur, Nafiseh Nafissi, Olla Wasfi, Katlyn Sheldon, Shawn Wettig, Roderick Slavcev

“…Recently, phages have been employed as gene-delivery vectors (phage nanomedicine), for nearly half a century as tools in genetic research, for about two decades as tools for the discovery of specific target-binding proteins and peptides, and for almost a decade as tools for vaccine development. …”
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsids by Taisuke Furusho, Ranjan Das, Hideyuki Hakui, Anusha Sairavi, Kei Adachi, Mia S. Galbraith-Liss, Pratheppa Rajagopal, Masahiro Horikawa, Shuhua Luo, Lena Li, Kentaro Yamada, Nicole Andeen, Gregory A. Dissen, Hiroyuki Nakai

“…Thus, this study provides mechanistic insights and underscores importance of context-dependent selection of AAV capsids to overcome challenges in gene delivery to the kidney.…”
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Reversible Ca2+ signaling and enhanced paracellular transport in endothelial monolayer induced by acoustic bubbles and targeted microbeads by Jiawei Lin, Chaofeng Qiao, Hao Jiang, Zhihui Liu, Yaxin Hu, Wei Liu, Yu Yong, Fenfang Li

“…This investigation provides new insights for ultrasound induced Ca2+ signaling and its influence on endothelial permeability, which may help develop new strategies for safe and efficient drug/gene delivery in the vascular system.…”
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Pancreatic Cancer: Molecular, Biochemical, Chemopreventive, and Therapeutic Aspects by Juan Iovanna, José Luis Neira

“…The bioavailability of compounds such as antisense oligonucleotides and siRNAs in humans remains a big hurdle, which will require further improvement of gene-delivery strategies. Finally, the long-term goal of the therapy individualization for patients is possible if factors that predict treatment response, such as biological markers, could be determined accurately. …”
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Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses by Tie-Lin Chen, Hong-Lian Wang, Yun-Hong Liu, Yin Fang, Rui-Zhi Tan, Pu-Hui Zhou, Qin Zhou, Xiao-Yan Lv

“…We for the first time employed and validated scAAV as a gene delivery tool in cultured kidney. Conclusions. These findings are expected to expedite the use of the ex vivo embryonic kidney cultures for kidney development research. …”
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Non-medical treatment for late age-related macular degeneration by A.K. Drakon, A.G. Kurguzova, V.M. Sheludchenko, N.B. Korchazhkina

“…This technique’s heart is a specific gene delivery to target cells to generate natural VEGF inhibitors. …”
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Three-dimensional linkage analysis with digital PCR for genome integrity and identity of recombinant adeno-associated virus by Tam Duong, Michele Firmo, Chien-Ting Li, Bingnan Gu, Peng Wang

“…Abstract Recombinant adeno-associated virus (rAAV) has emerged as the vector of choice for in vivo gene delivery, with numerous clinical trials underway for the treatment of various human diseases. …”
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Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach by Wei Zhu, Han Wang, Bin Feng, Guangli Liu, Yixin Bian, Tianhao Zhao, Qi Wang, Xisheng Weng

“…To enhance the scaffold's structural integrity and gene delivery efficiency, mineralized silk fibroin and miR140‐5p‐loaded MON‐PEI nanoparticles are incorporated. …”
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DNA-loaded targeted nanoparticles as a safe platform to produce exogenous proteins in tumor B cells by Maria Cristina Grimaldi, Sara Bozzer, Dick J. Sjöström, Dick J. Sjöström, Linnea I. Andersson, Tom Eirik Mollnes, Tom Eirik Mollnes, Per H. Nilsson, Per H. Nilsson, Luca De Maso, Federico Riccardi, Michele Dal Bo, Daniele Sblattero, Paolo Macor

“…This strategy has the advantage of minimizing off-target effects by restricting gene delivery to the desired cell population. However, the nanoplatform must guarantee both the local production of the protein and the safety of the treatment to allow an effective therapy with reduced systemic toxicity.MethodsIn order to ensure a selective delivery of nucleic acids, we developed poly(lactic-co-glycolic acid) (PLGA)-poly(vinyl alcohol) (PVA) NPs loaded with an Enhanced Green Fluorescent Protein (EGFP)-coding plasmid and covalently coated with antiCD19 recombinant antibody as a targeting mechanism. …”
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Identifying Multiomic Signatures of X‐Linked Retinoschisis‐Derived Retinal Organoids and Mice Harboring Patient‐Specific Mutation Using Spatiotemporal Single‐Cell Transcriptomics... by Yueh Chien, You‐Ren Wu, Chih‐Ying Chen, Yi‐Ping Yang, Lo‐Jei Ching, Bo‐Xuan Wang, Wei‐Chao Chang, I‐Hsun Chiang, Pong Su, Shih‐Yu Chen, Wen‐Chang Lin, I‐Chieh Wang, Tai‐Chi Lin, Shih‐Jen Chen, Shih‐Hwa Chiou

“…Furthermore, therapeutic targeting of the chronic ER stress/eIF2α pathway activation synergistically enhances the efficacy of AAV‐mediated RS1 gene delivery, ultimately improving bipolar cell integrity, postsynaptic transmission, disorganized retinal architecture, and electrophysiological responses. …”
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Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors by Xiujuan Zhang, Siyuan Hao, Zehua Feng, Kang Ning, Cagla Aksu Kuz, Shane McFarlin, Donovan Richart, Fang Cheng, Ander Zhang-Chen, Richenda McFarlane, Ziying Yan, Jianming Qiu

“…These findings highlight the involvement of the CMP-SIA transporter in the intracellular trafficking of rAAV vectors post-internalization.IMPORTANCErAAV is an essential tool for gene delivery in the treatment of genetic disorders; however, the mechanisms of rAAV transduction remain partially understood. …”
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