Unraveling the genetic spectrum of inherited deaf-blindness in Portugal by Telma Machado, Telmo Cortinhal, Ana Luísa Carvalho, Francisco Teixeira-Marques, Rufino Silva, Joaquim Murta, João Pedro Marques

“…A total of 55 genetic variants were identified across 16 different genes; 11 of these variants are novel and herein reported for the first time. …”
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Lipid-encapsulated gold nanoparticles: an advanced strategy for attenuating the inflammatory response in SARS-CoV-2 infection by Sanjeevram Dhandapani, Yujeong Ha, Rongbo Wang, Tae Woo Kwon, Ik-Hyun Cho, Yeon-Ju Kim

“…RNA sequencing analysis unveiled differentially expressed genes (DEGs) specifically involved in the ABC transporters signaling pathway. …”
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Longitudinal DNA methylation profiles in saliva of offspring from mothers with gestational diabetes: associations with early childhood growth patterns by Teresa M. Linares-Pineda, Alfonso Lendínez-Jurado, Alberto Piserra-López, María Suárez-Arana, María Pozo, María Molina-Vega, María José Picón-César, Sonsoles Morcillo

“…Functional analysis linked several of these DMPs to genes involved in inflammation and metabolic processes, including CYTH3 and FARP2, both implicated in growth and metabolic pathways. …”
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Animal experiments and network pharmacology to explore the anti-inflammatory mechanism of dapagliflozin in the treatment of polycystic ovary syndrome by Yong Liu, He Bai, Huilin Guan, Chunhua Wang, Xueqing Song, Zihao Yong, Xiaomeng Guo, Luxin Li, Zhen Zhang

“…Furthermore, network pharmacology identified 26 intersecting target genes relevant to inflammation in PCOS, with subsequent molecular docking simulations revealing strong binding affinities of dapagliflozin to key targets, including AKT1 and TP53.Conclusions These findings suggest that dapagliflozin exerts beneficial effects on PCOS by ameliorating ovarian dysfunction and reducing inflammation. …”
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Effects of Bacillus subtilis-fermented soybean meal replacing fish meal on antioxidant activity, immunity, endoplasmic reticulum stress and hepatopancreas histology in Pacific whit... by Songming Chen, Jieyu Dai, Yan Chen, Qiang Chen, Fen Dong, Congcong Wang, Yulong Sun, Jiteng Wang, Tao Han

“…FSBM substitution did not significantly affect the expression of immune genes in the intestine. In terms of endoplasmic reticulum stress, FSBM substitution significantly increased the mRNA expression of eif2α in the hepatopancreas. …”
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The prebiotic potential of dietary onion extracts: shaping gut microbial structures and promoting beneficial metabolites by Yebeen Yoo, Seongok Kim, WonJune Lee, Jinwoo Kim, Bokyung Son, Kwang Jun Lee, Hakdong Shin

“…Notably, short-chain fatty acid (SCFA)-producing bacteria, such as Bifidobacterium_388775, Feacalibacterium, and Fusicatenibacter, were overrepresented in response to onion extracts in enterotype B. Furthermore, genes related to butyrate production were significantly overrepresented in the onion-treated group within enterotype B. …”
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Long-term effects of combining anaerobic digestate with other organic waste products on soil microbial communities by Daniela Mora-Salguero, Denis Montenach, Manon Gilles, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau, Sophie Sadet-Bourgeteau

“…Soil microbial communities were characterized using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes, following DNA extraction from soil samples collected in 2022, six years after the initial digestate application.ResultsThe results indicated that combining OWPs rich in stable and recalcitrant organic matter, such as BIO and FYM, with raw digestate, offers an improved fertilization practice. …”
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Single-Cell RNA Sequencing Reveals LEF1-Driven Wnt Pathway Activation as a Shared Oncogenic Program in Hepatoblastoma and Medulloblastoma by Christophe Desterke, Yuanji Fu, Jenny Bonifacio-Mundaca, Claudia Monge, Pascal Pineau, Jorge Mata-Garrido, Raquel Francés

“…Among the commonly upregulated genes, the transcription factor LEF1 was significantly expressed in both tumor types. …”
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Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…The decrease in intestinal villus height, villous height: crypt depth ratio and digestibility was accompanied by lower expression of ZO1, ZO2, Claudin1, DMT1, CAT1, SGLT1 and PBD114 genes, as well as decreased enzyme activities of intestinal alkaline phosphatase, lactase, sucrase and maltase of piglets in DA group compared to piglets in DCON group. …”
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New approach to generating of human monoclonal antibodies specific to the proteolytic domain of botulinum neurotoxin A by Marina Vladimirovna Silkina, Alena Sergeevna Kartseva, Alena Konstantinovna Riabko, Mariia Aleksandrovna Makarova, Metkhun Madibronovich Rogozin, Yana Olegovna Romanenko, Igor Georgievich Shemyakin, Ivan Alekseevich Dyatlov, Victoria Valerievna Firstova

“…Hybridoma technology remains the preferred method for producing MAbs with naturally paired immunoglobulin genes and with preserved innate functions of immune cells. …”
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Identification, distribution, and hosts of Meloidogyne spp. infecting horticultural crops in Florida, USA with focus on Meloidogyne enterolobii by Riva Gabrieli, Brito Janete A., de Oliveira Clemen, Marin Marcus, Gu Mengyi, Bui Hung Xuan, Desaeger Johan

“…Many root-knot nematode (RKN) species in the genus Meloidogyne occur in Florida, including M. enterolobii, a species able to overcome RKN resistance genes in many crops. The distribution of these nematodes in horticultural crops is not well known. …”
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The Use of Coffee Cherry Pulp Extract as an Alternative to an Antibiotic Growth Promoter in Broiler Diets by Wanaporn Tapingkae, Phatchari Srinual, Pimporn Khamtavee, Naret Pintalerd, Thanongsak Chaiyaso, Mongkol Yachai, Chanidapha Kanmanee, Chompunut Lumsangkul, Orranee Srinual

“…(<i>p</i> < 0.05), and increased the expression of immune-related genes and antioxidant activity in the liver and intestines (<i>p</i> < 0.05). …”
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Vitamin D and exercise improve VEGF-B production and IGF-1 levels in diabetic rats: insights the role of miR-1 suppression by Fatemeh Mazaheri, Rastegar Hoseini, Ahmad Gharzi

“…The effects of an eight-week AT program and Vit D on the expression of miR-1, IGF-1 genes, and VEGF-B in the cardiomyocytes of rats with T2DM. …”
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Identification of determinants that allow maintenance of high-level fluoroquinolone resistance in Acinetobacter baumannii by Efrat Hamami, Wenwen Huo, Juan Hernandez-Bird, Arnold Castaneda, Jinna Bai, Sapna Syal, Juan C. Ortiz-Marquez, Tim van Opijnen, Edward Geisinger, Ralph R. Isberg

“…Disruption of the PAA pathway resulted in cytosolic acidification and defective expression of genes involved in protection from oxidative stress. …”
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ConvXGB: A novel deep learning model to predict recurrence risk of early-stage cervical cancer following surgery using multiparametric MRI images by Ji Wu, Jian Li, Bo Huang, Sunbin Dong, Luyang Wu, Xiping Shen, Zhigang Zheng

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Consequences of insecticide overuse in Hungary: assessment of pyrethroid resistance in Culex pipiens and Aedes albopictus mosquitoes by Rebeka Csiba, Zsaklin Varga, Dorina Pásztor, Bianka Süle, Vera Ihuoma Ogoke Mxinwa, Zoltán Soltész, Brigitta Zana, Krisztián Bányai, Gábor Kemenesi, Kornélia Kurucz

“…In contrast, for Ae. albopictus, the analyzed individuals were found to carry only the susceptible alleles, indicating a homozygous susceptible genotype across the investigated populations on the basis of V1016G and F1534C genes. Conclusions Our work highlights the consequences of the unilateral and long-term use of chemical treatments on mosquitoes. …”
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MSC-EVs and UCB-EVs promote skin wound healing and spatial transcriptome analysis by Ruonan Li, Haotian Wang, Xiaolong Wang, Yanbin Yang, Kai Zhong, Xuemei Zhang, Heping Li

“…ST analysis of skin tissue from each group after wound healing revealed that MSC-EVs and UCB-EVs reduced the relative expression of marker genes in myofibroblasts, regulated wound healing, and decreased scar formation by reducing the expression of the TGF-β signaling pathway and increasing the expression of the Wnt signaling pathway. …”
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Application of Targeted Next-Generation Sequencing in Bronchoalveolar Lavage Fluid for the Detection of Pathogens in Pulmonary Infections by Dai X, Xu K, Tong Y, Li J, Dai L, Shi J, Xie H, Chen X

“…Furthermore, a total of 61 cases of antimicrobial resistance genes were detected, including 34 cases of 23S rRNA A2063G, 6 cases of KPC, 5 cases of OXA, 2 cases of CTX-M, 3 cases of IMP, 1 case of NDM and 13 cases of mecA. …”
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Genetic Biomarkers and Circulating White Blood Cells in Osteoarthritis: A Bioinformatics and Mendelian Randomization Analysis by Yimin Pan, Xiaoshun Sun, Jun Tan, Chao Deng, Changwu Wu, Georg Osterhoff, Nikolas Schopow

“…<b>Results</b>: Total of 233 OA-related genes were identified, showing significant enrichment in pathways associated with WBC function. …”
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Tp53 determines the spatial dynamics of M1/M2 tumor-associated macrophages and M1-driven tumoricidal effects by Yi-Jing Hsiao, Min-Shu Hsieh, Gee-Chen Chang, Yin-Chen Hsu, Chia-Yu Wang, Yan-Ming Chen, Yi-Ling Chen, Pan-Chyr Yang, Sung-Liang Yu

“…EGFR and TP53 are among the most frequently mutated genes in lung adenocarcinoma. We characterized the mutation status and density of M1/M2 TAMs within tumor islets and stroma in 117 lung adenocarcinomas using next-generation sequencing and immunohistochemistry, respectively. …”
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