Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 by Kimberly A Nichol, Melissa McCracken, Melanie R DeCorby, Kristjan Thompson, Michael R Mulvey, James A Karlowsky, Daryl J Hoban, George G Zhanel

“…Strain typing was performed by pulsed-field gel electrophoresis (PFGE) and the mecA, nuc and pvl genes were detected by polymerase chain reaction. …”
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Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

“…Results: Compared with the control group, TPTLN and sodium pentobarbital synergistically increased the sleep onset rate to 100%, significantly shortened the sleep latency by 31.7% (P < 0.05), and extended the sleep duration 2.1 times (P < 0.05); increased the GABA/Glu ratio in the serum and brain tissue by 68.8% and 19.3%, respectively and significantly improved the excitation/inhibition imbalance (P < 0.001, P < 0.000 1) as evidenced by a 49.4% increase in the serum GABA level (P < 0.001), a 7.6% increase in the brain 5-HT level, and a 24.3% and 23.4% decrease in the brain levels of cortisol and NE (P < 0.05); and significantly up-regulated the expression levels of the GABAA-α1 and 5-HT1A genes 1.5 and 1.3 times, respectively (P < 0.01, P < 0.05). …”
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Staphylococcus aureus vesicles impair cutaneous wound healing through p38 MAPK-MerTK cleavage-mediated inhibition of macrophage efferocytosis by Jiaxin Ou, Kangxin Li, Hui Yuan, Shaohua Du, Tingting Wang, Qiannan Deng, Huimei Wu, Weiyan Zeng, Kui Cheng, Kutty Selva Nandakumar

“…SAVs inhibit efferocytosis by activating the TLR2-MyD88-p38 MAPK signaling pathway, which regulates efferocytosis receptor genes. This activation promoted cleavage and shedding of MerTK, a crucial receptor for macrophage-driven efferocytosis. …”
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Circular RNA encoded by PPARG in the peripheral blood and a lipopolysaccharide-induced cardiomyocyte inflammation model is identified as a marker of fulminant myocarditis by Shasha Huang, Shumin He, Fei Xiao, Yang Zhou, Su Lyu

“…GO and KEGG analysis revealed that the genes encoding the dysregulated circRNAs were associated with various biological functions related to the risk of FM development, including infectious diseases, the immune system, and signal transduction. …”
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Copper Does Not Induce Tenogenic Differentiation but Promotes Migration and Increases Lysyl Oxidase Activity in Adipose-Derived Mesenchymal Stromal Cells by Marta Milewska, Anna Burdzińska, Katarzyna Zielniok, Katarzyna Siennicka, Sławomir Struzik, Piotr Zielenkiewicz, Leszek Pączek

“…The treatment with CuSO4 did not positively affect the expression of genes associated with tenogenesis (COL1α1, COL3α1, MKX, and SCX). …”
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NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis by Jung Hwan Yoon, Jeong-Kyu Kim, Jung Woo Eun, Hassan Ashktorab, Duane T. Smoot, Suk Woo Nam, Won Sang Park

“…Quantitative real-time PCR and Western blot analyses were conducted to assess the expression of mitosis-related genes and proteins. Flow cytometry was utilized to evaluate cell cycle distribution, while immunofluorescence microscopy enabled the visualization of mitotic abnormalities. …”
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Physiological and transcriptome analysis reveals the mechanism of Gymnocarpos przewalskii response to drought stress by Chao Du, Xinyu Ni, Mengjiao Yan, Qinghan Meng, Junying He

“…Comparative analysis identified 3,015 differentially expressed genes under osmotic stress. There were 2,134 and 1,739 DEGs enriched in 47 GO secondary categories and 129 KEGG pathways, respectively. 2 up-regulated DEGs were annotated to P5CS, a key enzyme in the biosynthesis of proline. 32 DEGs were annotated to various antioxidases and antioxidants. 81 DEGs were annotated to 8 plant hormone signaling pathways, in which the auxin and ABA signaling pathways exhibited dominant enrichment. 150 DEGs were annotated to 35 transcription factor families with the abundant enrichment of TF families containing WRKY, bZIP, ERF, bHLH, MYB, and NAC. …”
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Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells by Sukannika Tubtimsri, Tiraniti Chuenbarn, Suwisit Manmuan

“…Ultimately, the mechanisms of action of the apoptotic markers and genes implicated in the metastatic process were clarified. …”
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Assessing the impact of sewage and wastewater on antimicrobial resistance in nearshore Antarctic biofilms and sediments by Melody S Clark, Benjamin H Gregson, Carla Greco, Harisree Paramel Nair, Marlon Clark, Claire Evans, Kevin A. Hughes, Kudzai Hwengwere, Marcus Leung, Lloyd S Peck, Caray A. Walker, William Chow

“…Results Amplicon sequencing of biofilms and sediments from the vicinity of Rothera Research Station revealed highly variable and diverse microbial communities. Analysis of AMR genes generated from long-reads Nanopore MinION sequencing showed similar site variability in both drug class and resistance mechanism. …”
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Puerarin Ameliorates the Ferroptosis in Diabetic Liver Injure Through the JAK2/STAT3 Pathway Inhibition Based on Network Pharmacology and Experimental Validation by Fan X, Liu S, Yu J, Hua J, Feng Y, Wang Z, Shen Y, Lan W, Wang J

“…Protein-protein interaction (PPI) network and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used to predict key targets and pathways. …”
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Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients by Wang T, Zhang L, Chen W, Long Y, Zhang Y, Wang L, Hou Z

“…We aim to investigate the change of cells from deep fascia in response to NF by single-cell RNA-seq.Methods: Fascia samples from NF patients (NF group, NG, n = 3) and volunteer (control group, CG, n = 4) were obtained and we utilized scRNA-seq to observe the variation of cells and differentially expressed genes. Then, multiplex staining and multispectral imaging and immunohistochemistry were used to be further verified.Results: Our findings showed that three fibroblast subclusters (antigen-presenting Fib, mesenchymal Fib, and myoFib) and three macrophage subclusters (SPP1+ Mac0, IL1B+ Mac1, and SPP1+M2) were found to have increased proportions with distinct roles in NF patients. …”
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Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway by Chen Feng, Wei Mao, Chenyang Yuan, Pin Dong, Yuying Liu

“…Differentially expressed genes are subjected to pathway enrichment analysis, and MEK/ERK protein expression and phosphorylation are validated via western blot. …”
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Adipose-derived mesenchymal stem cells combined with platelet-rich plasma are superior options for the treatment of osteoarthritis by Weijie He, Jie Zhao, Jiafei Liu, Fangxing Wang, Zhenyu Xu

“…Expression levels of these genes were significantly lower in Groups C–G than in Group B (P < 0.05). …”
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A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure by Muhammad K. Hakeem, Asma Al-Menhali, Sampath K. Elangovan, Iltaf Shah

“…IntroductionNumerous physiological systems, such as the functioning of the immune system, bone health, and the regulation of expression of genes, depend critically on vitamin D. Considering the significance of vitamin D for health, it is critical to understand how it is metabolized and the factors that affect its levels.MethodsThe objective of this study was to develop and validate an LC-MS/MS method to examine the effects of light exposure and dietary vitamin D consumption on the levels of vitamin D and its metabolites in a mouse model under consistent growth conditions throughout the year. …”
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The total extract of Abelmoschus manihot (L.) medic flowers (TEA) mediated Nrf2-TFAM signalling to regulate mitochondrial antioxidant mechanism by Ying Song, Xinyi Zhu, Beibei Wang, Qisong Li, Biwei Song

“…In addition the expression of mitochondrial respiratory chain genes and proteins decreased. TEA promoted the expression of Nrf2 in HaCaT cells, activated the downstream antioxidant response, and alleviated the oxidative stress and mitochondrial damage caused by H2O2. …”
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Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex by Froylan Sosa, Jeremy Block, Yao Xiao, Peter J. Hansen

“…Earlier research indicates that CSF2 can regulate genes involved in cellular stress responses and block apoptosis. …”
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Polymorphisms and dental age in non-syndromic cleft lip and palate: a cross-sectional study by Gabriela Fonseca-Souza, Lhorrany Alves-Souza, Maria Angélica Hueb de Menezes-Oliveira, Nikolaos Daratsianos, Svenja Beisel-Memmert, Christian Kirschneck, Rafaela Scariot, Juliana Feltrin-Souza, Erika Calvano Küchler

“…The purpose of this cross-sectional study was to compare the dental age (DA) between children with and without CL ± P, and whether single nucleotide polymorphisms (SNPs) in genes encoding growth factors are associated with DA variations. …”
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LncRNA GAS5 inhibits glioma progression through miR-135b-5p/APC axis by ZHANG Jidong, WANG Yutao, JI Jianwen

“…Western blotting and qRT-PCR were applied to measure the expression of related genes in glioma tissues or cell lines. CCK-8, Transwell and wound healing assays was conducted to determine the effect of GAS5 on the viability and motility of glioma cells. …”
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Anti-Inflammatory and Anti-Migratory Effects of Morin on Non-Small-Cell Lung Cancer Metastasis via Inhibition of NLRP3/MAPK Signaling Pathway by Punnida Arjsri, Kamonwan Srisawad, Sonthaya Umsumarng, Pilaiporn Thippraphan, Songyot Anuchapreeda, Pornngarm Dejkriengkraikul

“…RT-qPCR measurement quantified the expression of inflammatory genes, and zymography and Western blotting were used to examine changes in invasive protein levels, epithelial-to-mesenchymal transition (EMT) markers, and underlying molecular mechanisms. …”
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The Mla pathway promotes Vibrio cholerae re-expansion from stationary phase by Deborah R. Leitner, Franz G. Zingl, Alexander A. Morano, Hailong Zhang, Matthew K. Waldor

“…Here, we performed a transposon insertion site sequencing loss-of-function screen to identify Vibrio cholerae genes that promote pathogen fitness in stationary phase. …”
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