Insights into the mechanisms of serplulimab: a distinctive anti-PD-1 monoclonal antibody, in combination with a TIGIT or LAG3 inhibitor in preclinical tumor immunotherapy studies by Yizhou Zhang, Ruicheng Wei, Ge Song, Xinyi Yang, Mengli Zhang, Wei Liu, Aiying Xiong, Xuehan Zhang, Qianhao Li, Wan-Jen Yang, Chencheng Han, Rui Liu, Chen Hu, Qingyu Wang, Jun Zhu, Yongqiang Shan

“…Mechanistically, the serplulimab combination effectively reduces tumor microenvironment Treg cell populations, augments effector and memory T cell populations, and more potently modulates genes associated with diverse facets of the immune system, surpassing the effects of the pembrolizumab combination. …”
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EDF1 accelerates ganglioside GD3 accumulation to boost CD52-mediated CD8+ T cell dysfunction in neuroblastoma by Di Li, Meng Li, Zhenjian Zhuo, Huiqin Guo, Weixin Zhang, Yile Xu, Hai-Yun Wang, Jiabin Liu, Huimin Xia, Huiran Lin, Jue Tang, Jing He, Lei Miao

“…It was verified that high EDF1 expression in NB cells led to Lactosylceramide (LacCer) accumulation, as well as downstream ganglioside-GD3, which subsequently increased the expression of CD52 and immune checkpoint genes, chemotaxis, and apoptosis-related events in activated CD8+T cells. …”
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Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails by Veronica Rivi, Anuradha Batabyal, Cristina Benatti, Fabio Tascedda, Johanna Maria Catharina Blom, Ken Lukowiak

“…While both cold and heat shock upregulated the HSP levels in the snail's central ring ganglia, cold exposure did not affect the expression levels of the neuroplasticity genes LymGRIN1 and LymCREB1, contrasting with heat shock's neurogenic effects. …”
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Production of subtilisin proteases in bacteria and yeast by A. S. Rozanov, S. V. Shekhovtsov, N. V. Bogacheva, E. G. Pershina, A. V. Ryapolova, D. S. Bytyak, S. E. Peltek

“…Despite longstanding applications of microbial proteases and a large number of research papers, the search for new protease genes, the construction of producer strains, and the development of methods for their practical application are still relevant and important, judging by the number of citations of the research articles on proteases and their microbial producers. …”
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Brief Warm and Aldo-Keto Reductase Family <i>AspiAKR1B1</i> Contribute to Cold Adaptation of <i>Aleurocanthus spiniferus</i> by Zhi-Fei Jia, Yan-Ge Cui, Meng-Yuan Liu, Jeremiah Joe Kabissa, Yong-Yu Xu, Zhi-Wei Kang, Zhen-Zhen Chen

“…The expression profile of cold-resistant genes indicated that the aldo-keto reductase family 1 member B1 in <i>Aleurocanthus spiniferus</i> (<i>AspiAKR1B1</i>) shows a significant decrease at the end of the overwintering period. …”
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Cytoplasmic genetic diversity of potato varieties bred in Russia and FSU countries by Т. A. Gavrilenko, N. S. Klimenko, N. V. Alpatieva, L. I. Kostina, V. A. Lebedeva, Z. Z. Evdokimova, O. V. Apalikova, L. Y. Novikova, O. Yu. Antonova

“…Fragments of the  nad2, nad7, cox2, atp6 and  CcmFc genes  were identical  in all 15 selected genotypes. …”
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Integrated multiomics approach and pathological analyses provide new insights into hepatic injury and metabolic alterations in Saanen goats after dietary exposure to aflatoxin B1 by Donghua Su, Jing Peng, Jingjing Hao, Xi Wang, Peiqiang Yu, Shengli Li, Haitao Shi

“…We identified 251, 269, and 154 significant differentially expressed genes (DEG) and 340, 596, and 127 significant differential metabolites in comparisons between the control (CON; 0 μg/kg) and low-dose (LO; 50 μg/kg) groups, the CON and high-dose (HI; 500 μg/kg) groups, and the LO and HI groups, respectively. …”
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Frequency and Molecular Characteristics of Mismatch Repair-deficient Status among Multiple Synchronous Colorectal Cancers by Kenichi Chikatani, Noriyasu Chika, Noriko Tanabe, Yoshiko Mori, Okihide Suzuki, Takatoshi Matsuyama, Keiichiro Ishibashi, Hidetaka Eguchi, Yasushi Okazaki, Tatsuro Yamaguchi, Hideyuki Ishida

“…When necessary, MLH1-methylation analysis and testing of germline and somatic MMR genes were performed. Results: In total, 133 patients (33 females) had 309 lesions. …”
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Mild ultrasound-assisted alkali de-esterification modified pectins: Characterization and structure–activity relationships in immunomodulatory effects by Huan Guo, Dong Li, Baohe Miao, Kanglin Feng, Guijing Chen, Renyou Gan, Zhiliang Kang, Hong Gao

“…Additionally, AP-E3 significantly upregulated the expression of key immune response genes (tnf-α, il-1β, il-6, cox-2, inos, and nf-κb) and restored the gut microbiota composition and abundance in chloramphenicol-induced immunocompromised zebrafish. …”
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Celastrol boosts fluconazole efficacy against vaginal candidiasis: in vitro and in vivo evidence by Fatma Al-zahraa A. Yehia, Hisham A Abbas, Tarek M. Ibrahim, Basem Mansour, Zuhier A. Awan, Mohammed W. Al-Rabia, Wesam H. Abdulaal, Mustafa Adnan Zeyadi, Solomon Z. Okbazghi, Tarek S. Ibrahim, Wael A. H. Hegazy, Salwa E. Gomaa

“…Quantification of m-RNA levels of efflux-mediated azole resistance genes within azole-resistant C. albicans demonstrated CDR1 overexpression. …”
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Genetic characteristics and pathogenesis of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus isolated from poultry in South Korea, 2022–2023 by Ra Mi Cha, Min-Ji Park, Yoon-Gi Baek, Yu-Na Lee, Yunyueng Jang, Yong-Myung Kang, Gyeong-Beom Heo, Se-Hee An, Kwang-Nyeong Lee, Jae-Kyeom Kim, Hye-Ryoung Kim, Youn-Jeong Lee, Eun-Kyoung Lee

“…Phylogenetic analysis of hemagglutinin genes revealed that the South Korean H5N1 HPAIV isolates were closely related to Eurasian and American HPAIVs isolated between 2022 and 2023. …”
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Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis by Jane Carr-Wilkinson, Nilendran Prathalingam, Deepali Pal, Mohammad Moad, Natalie Lee, Aishwarya Sundaresh, Helen Forgham, Peter James, Mary Herbert, Majlinda Lako, Deborah A. Tweddle

“…After 7 days of PA6 cell coculture, mRNA expression of SNAIL and SOX-9 neural crest specifier genes and the neural marker peripherin (PRPH) increased. …”
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A DNA-Methylated Sight on Autoimmune Inflammation Network across RA, pSS, and SLE by Xingqiang Wang, Dongyun Lei, Jie Ding, Shuang Liu, Li Tao, Fan Zhang, Jiangyun Peng, Jian Xu

“…In this study, we integrated 5 Cytokine-Chips from genome-wide DNA methylation datasets of the 3 kind of AIDs, delta-beta value was calculated for intergroup difference, and comprehensive bioinformatics analyses of cytokine genes with aberrant methylations were performed. 125 shared differential methylation variabilities (DMVs) were identified. …”
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Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation by Yu Ga, Yuanyuan Wei, Qingyu Zhao, Yimeng Fan, Yannan Zhang, Zhifang Zhang, Sijia Hao, Lixia Wang, Zhifen Wang, Jinlong Han, Shuang Wu, Zhihui Hao

“…NP results showed that caspase-1 was the most dysregulated of the core genes associated with UC. A PR dose of 0.136 mg/g administered to DSS treated mice reversed weight loss and decreased colon lengths found in UC mice. …”
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MicroRNA-26b inhibits cardiac remodeling after myocardial infarction by targeting ring finger protein 6 expression by Chun-Mei Tang, Qiang Su, Hai-Xia Zhao, He-Huan Sui, Jing Liang, Li-Sha Zhu, Si-Yun Yang, Tao Liu

“…Microarray data were analyzed to distinguish differentially expressed genes in MI. miR-26b was found to be poorly expressed, whereas ring finger protein 6 (RNF6) was highly expressed in MI. …”
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Prognosis conferred by molecular features of appendix-derived Pseudomyxoma Peritonei by Ruiqing Ma, Guojun Li, Yingjiang Ye, Lei Liang, Chong Wang, Haipeng Zhou, Pu Zhang, Lubiao An, Guanjun Shi, Qian Chen, Hongbin Xu, Zhidong Gao

“…Patients with tumor mutational burden (TMB) > 1(P = 0.026) or alterations in TP53 (P = 0.006) or SMAD4 (P = 0.013) had significantly worse OS compared to those with a TMB < 1 or normal genes. Patients with homologous recombination deficiency (HRD) positivity (P = 0.003) or alterations in TGFBR2 (P = 0.037) experienced worse PFS compared to their respective control groups. …”
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Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep by Ting Liu, Chenlei Wang, Mirco Corazzin, Taiwu Zhang, Maoqin Zhai, Yue Zhang, Qiaoge Zhang, Xin Zhao, Ye Jin, Lina Sun

“…The L. reuteri group showed significantly higher expression of genes encoding colonic epithelial nutrient transport (GPR43) and immune barrier function (Zonulin) than the control group. …”
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Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach by Fathia Ben-Rached, Amit Kumar Subudhi, Chang Li, Mariah Alawi, Rohit Satyam, Sui Xu, Guoding Zhu, Raeece Naeem, Sara Mfarrej, Di Liu, Zenaida Stead, Caroline Askonas, Yaobao Liu, Jun Cao, Arnab Pain

“…Consequently, the detection of thousands of additional SNPs not detectable in pre-SWGA samples was facilitated after SWGA, allowing more substantial downstream population genomics analysis, particularly for the polymorphic and antimalarial genes of great interest for all the species. Furthermore, leveraging the long DNA fragments generated by SWGA, we achieved high-quality genome assemblies for P. malariae and P. ovale using PacBio long reads sequencing technology. …”
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Exogenous cystine increases susceptibility of drug-resistant Salmonella to gentamicin by promoting oxidation of glutathione metabolism and imbalance of intracellular redox levels by Junyuan Du, Junyuan Du, Zhiyi Wu, Zhiyi Wu, Chunyang Zhu, Chunyang Zhu, Heng Yang, Heng Yang, Feike Zhao, Feike Zhao, Binghu Fang, Binghu Fang

“…The expression of glutathione and stress genes was determined using real-time quantitative PCR.ResultWe confirmed that exogenous cystine increased the lethal effect of gentamicin against strain S. enterica Typhimurium (ATCC 14028) and other clinically resistant Salmonella serotypes. …”
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Risk Factors and Circulating Subtypes of <i>Cryptosporidium</i> spp. and <i>Giardia duodenalis</i> in Hospitalized Children in Mozambique by Ofélia Nhambirre, Maria Luísa Lobo, Idalécia Cossa-Moiane, Adilson Bauhofer, Nilsa de Deus, Olga Matos

“…In this study, two nested PCRs targeting <i>ssurRNA</i> and <i>gp60</i> genes were used for genetic diversity of <i>Cryptosporidium</i> spp. and <i>b-giardin</i> for <i>G. duodenalis</i>. …”
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