CD21low B cells reveal a unique glycosylation pattern with hypersialylation and hyperfucosylation by Peter Tobias Felixberger, Peter Tobias Felixberger, Geoffroy Andrieux, Andrea Maul-Pavicic, Andrea Maul-Pavicic, Sigune Goldacker, Sigune Goldacker, Ina Harder, Ina Harder, Sylvia Gutenberger, Sylvia Gutenberger, Jonathan J. M. Landry, Vladimir Benes, Till Fabian Jakob, Melanie Boerries, Melanie Boerries, Lars Nitschke, Reinhard Edmund Voll, Reinhard Edmund Voll, Klaus Warnatz, Klaus Warnatz, Baerbel Keller, Baerbel Keller

“…Additionally, we examined the expression of glycosylation-related genes by RNAseq in naïve-like CD21low B cells ex vivo, as well as in naïve CD21pos B cells from healthy controls after in vitro stimulation.ResultsUnlike CD21pos B cells, naïve-like CD21low B cells from CVID patients and CD21low B cells from healthy controls exhibited a unique glycosylation pattern with high levels of α2,6 sialic acids and fucose. …”
Get full text

Genome-Wide Association Study of Bone Mineral Density in Korean Men by Ye Seul Bae, Sun-Wha Im, Mi So Kang, Jin Hee Kim, Soon Hang Lee, Be Long Cho, Jin Ho Park, You-Seon Nam, Ho-Young Son, San Deok Yang, Joohon Sung, Kwang Ho Oh, Jae Moon Yun, Jong Il Kim

“…Additionally, we analyzed the relationship between BMD and five genes previously identified in Korean men. Rs61382873 (p = 0.0009) in LRP5, rs9567003 (p = 0.0033) in TNFSF11 and rs9935828 (p = 0.0248) in FOXL1 were observed for LS-BMD. …”
Get full text

Taxonomic composition and biodiversity of the gut microbiome from patients with irritable bowel syndrome, ulcerative colitis, and asthma by A. Y. Tikunov, A. N. Shvalov, V. V. Morozov, I. V. Babkin, G. V. Seledtsova, I. O. Voloshina, I. P. Ivanova, A. V. Bardasheva, V. V. Morozova, V. V. Vlasov, N. V. Tikunova

“…In this study, a comparative assessment of the biodiversity and taxonomic structure of gut microbiome was conducted based on the sequencing of 16S rRNA genes obtained from fecal samples of patients with IBS, UC, BA and volunteers. …”
Get full text

Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam by Huan Vinh Dong, Paul Adamson, Dieu Hoa Pham, Quynh Hoa Pham, Hai Ha Long Le, Loc Quang Pham, Hao Thi Minh Bui, Giang Minh Le, Jeffrey D. Klausner

“…Commensal Neisseria species in the oropharynx are an important reservoir of AMR genes that are transferred to N. gonorrhoeae, yet few data about AMR among commensal Neisseria in populations at risk for AMR exist. …”
Get full text

Immunogenicity and protective efficacy of a multi-antigenic adenovirus-based vaccine candidate against Mycobacterium tuberculosis by Jin-Seung Yun, Jin-Seung Yun, Eunkyung Shin, Young-Ran Lee, Jung-Ah Lee, Hyeokjin Lee, Jong-Seok Kim, Sung Jae Shin, Sang-Jun Ha, Sang-Won Lee, Dokeun Kim, Jung-Sik Yoo, Hye-Sook Jeong

“…RNA sequencing of the whole blood of rAd-TB4-vaccinated mice collected pre- and, 1 and 4 weeks post-infection, identified differentially expressed genes associated with immune and inflammatory responses, especially those in the Wnt signaling pathway.ConclusionOur results indicate that rAd-TB4 immunization enhances the immune response to the vaccine boosting antigens in BCG-primed mice, making it a potential adult pulmonary TB vaccine candidate.…”
Get full text

Cx43 Inhibition Attenuates Sepsis-Induced Intestinal Injury via Downregulating ROS Transfer and the Activation of the JNK1/Sirt1/FoxO3a Signaling Pathway by Zhaowei Zou, Bin Liu, Lisi Zeng, Xianzi Yang, Renli Huang, Cheng Wu, Huijuan Zhu, Yi Gao, Dongdong Yuan, Jinlong Yu

“…FoxO3a directly affected its downstream target genes, Bim and Puma, which are responsible for cell or tissue apoptosis. …”
Get full text

Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment by Sunisa Khongthong, Pipat Piewngam, Natthrit Roekngam, Prapot Maliwan, Sonsawan Kongpuckdee, Juthatip Jeenkeawpleam, Prawit Rodjan

“…Furthermore, at d 14 and 35, mRNA expressions of occludin, claudin-1, and tight junction protein-1 in the jejunum were upregulated (P < 0.05), and expression levels of five inflammatory cytokine genes were downregulated in the ileum (P < 0.05). …”
Get full text

Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece by Anestis Tsitsos, Alexandros Damianos, Konstantinos Kiskinis, Vasilios Tsiouris, Ilias Tirodimos, Nikolaos Soultos, Anna Papa, Vangelis Economou

“…All strains were multidrug-resistant and most carried ESBL/AmpC genes. Most <i>E. coli</i> isolates belonged to phylogroups A (41.4%, n = 36) and B1 (24.1%, n = 21). …”
Get full text

Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis by Min-Jeong Kim, Kyu-Min Kim, Jeong-Ih Shin, Jong-Hun Ha, Dong-Hae Lee, Jeong-Gyu Choi, Jin-Sik Park, Jung-Hyun Byun, Jung-Wan Yoo, Seokyong Eum, Myunghwan Jung, Seung Chul Baik, Woo Kon Lee, Hyung Lyun Kang, Min-Kyoung Shin

“…Variable regions of the genes 16S rRNA, rpoB, hsp65, and 16S-23S rRNA internal transcribed spacer were included in the analysis. …”
Get full text

Platinum drugs upregulate CXCR4 and PD‐L1 expression via ROS‐dependent pathways, with implications for novel combined treatment in gastric cancer by Xiaoyu Kang, Lin Zhang, Shushang Liu, Fei Wang, Haiming Liu, Fenli Zhou, Fei Wu, Haohao Zhang, Daiming Fan, Yongzhan Nie, Zhangqian Chen

“…Cisplatin‐induced CXCR4 upregulation relies on ROS/HIF‐1α and ROS/NF‐κB pathways, while cisplatin‐induced PD‐L1 upregulation is cyclic GMP‐AMP synthase/stimulator of IFN genes‐dependent and associated with elevated ROS levels in GC cells. …”
Get full text

Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) by Faji Li, Can Guo, Qi Zhao, Weie Wen, Shengnan Zhai, Xinyou Cao, Cheng Liu, Dungong Cheng, Jun Guo, Yan Zi, Aifeng Liu, Jianmin Song, Jianjun Liu, Jindong Liu, Haosheng Li

“…Additionally, this study identified seven candidate genes that encode disease resistance proteins, the BTB/POZ domains, peroxidase activity, and leucine-rich repeat receptor-like protein kinase. …”
Get full text

Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school children in Kenya by Victor Osoti, Kevin Wamae, Leonard Ndwiga, Paul M. Gichuki, Collins Okoyo, Stella Kepha, Kibor Keitany, Regina Kandie, Stephen Aricha, Rosebella Kiplagat, Charles Mwandawiro, Philip Bejon, Robert W. Snow, Lynette Isabella Ochola-Oyier

“…Methods In this study, we used Targeted Amplicon Deep Sequencing (TADS) to characterise the genetic diversity of the Pfk13, Pfdhfr, Pfdhps, and Pfmdr1 genes among primary school-going children in 15 counties in Kenya (Bungoma, Busia, Homa Bay, Migori, Kakamega, Kilifi, Kirinyaga, Kisii, Kisumu, Kwale, Siaya, Tana River, Turkana, Vihiga and West Pokot). …”
Get full text

Candidiasis mucocutánea crónica, infecciones micobacterianas y rosácea en un adulto mexicano con aumento de la función en STAT1 by Valeria Valerio-Gómez, Uriel Pérez-Blanco, Guillermo Velázquez-Sámano, Andrea Aída Velasco-Medina, Antonio Albarrán, Itzel Yoselín Sánchez, Laura Berrón-Ruiz, Sara Espinosa-Padilla, Lizbeth Blancas-Galicia

“…El diagnóstico se corroboró con la secuenciación de múltiples genes asociados con errores innatos de la inmunidad. …”
Get full text

Epigenetic «probes» for lung cancer monitoring: LINE-1 methylation pattern in blood- circulating DNA by A. A. Ponomaryova, Е. Y. Rykova, N. V. Cherdyntseva, A. A. Bondar, A. Y. Dobrodeev, A. A. Zavyalov, S. A. Tuzikov, L. O. Bryzgalov, T. I. Merkulova, V. V. Vlassov, P. P. Laktionov

“…Malignant cell transformation is accompanied by two processes of DNA methylation changes: promoter hypermethylation of specific genes and hypomethylation of retrotransposons. The composition of circulating DNA (cirDNA) from plasma and cell-surface-bound circulating DNA (csb- cirDNA) was shown earlier to be altered in the blood of cancer patients due to accumulation of tumor- specific aberrantly methylated DNA fragments, which are currently considered valuable cancer markers. …”
Get full text

Lipoxin A4 yields an electrophilic 15-oxo metabolite that mediates FPR2 receptor-independent anti-inflammatory signaling by Adolf Koudelka, Gregory J. Buchan, Veronika Cechova, James P. O’Brien, Emily R. Stevenson, Crystal E. Uvalle, Heng Liu, Steven R. Woodcock, Steven J. Mullett, Cheng Zhang, Bruce A. Freeman, Stacy L. Gelhaus

“…Alternatively, 15-oxo-LXA4, an electrophilic α,β-unsaturated ketone, alkylates nucleophilic amino acids and can modulate redox-sensitive transcriptional regulatory protein and enzyme function. 15-oxo-LXA4 activated nuclear factor (erythroid related factor 2)-like 2-regulated expression of anti-inflammatory and repair genes and inhibited NF-κB-regulated pro-inflammatory mediator expression. …”
Get full text

Metabolome and transcriptome analysis reveal the pigments biosynthesis pathways in different color fruit peels of Clausena lansium L. Skeels by Zhichang Zhao, Mark Owusu Adjei, Ruixiong Luo, Huaping Yu, Yali Pang, Jian Wang, Yu Zhang, Jun Ma, Aiping Gao

“…It aims to identify metabolic pathways and differentially expressed genes related to flavonoids and anthocyanins biosynthesis.ResultsThe PP (purple peel) has higher levels of a-carotene and b-carotene but lower levels of chlorophyll a, chlorophyll b, and lutein compared to BP. …”
Get full text

Effects of cage size on the natural behavior, serum parameters, production performance and hypothalamic mRNA expression profiles of laying hens by Yi Wan, Ruiyu Ma, Renrong Qi, Dawei Jiang, Helong Guan, Wei Liu, Junying Li, Yan Li, Kai Zhan

“…In total, 437 and 81 differentially expressed genes (DEGs) were identified in the hypothalamus of birds between the LC and SC groups and the MC and SC groups, respectively. …”
Get full text

Oxidative damage to DNA, expression of Mt-1, and activation of repair mechanisms induced by vanadium trioxide in cultures of human lymphocytes by V.A. Alcántara-Mejía, A.A. Beltrán-Flores, R.A. Mateos-Nava, L. Álvarez-Barrera, I.U. Bahena-Ocampo, E. Santiago-Osorio, E. Bonilla-González, J.J. Rodríguez-Mercado

“…The expression levels of sensor proteins (ATM and ATR) involved in DNA damage were determined via Western blotting, and the mRNA expression levels of metallothionein 1 (Mt-1) and genes involved in DNA repair (OGG1, APE1, XPB, XPD, MRE11, RAD50, Ku70, and Ku80) were estimated via RT-PCR and qPCR. …”
Get full text

Promotion of microfracture-mediated cartilage repair by the intra-articular injection of Mg2+ by Zhian Chen, Tianhua Zhou, Zhengbo Yin, Peiya Duan, Ying Zhang, Yujiao Feng, Rongmao Shi, Yongqing Xu, Rongqing Pang, Hongbo Tan

“…The expression levels of related genes were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). …”
Get full text

Efficacy and safety of pseudolaric acid B against Echinococcus multilocularis in vitro and in a murine infection model by Zhuo-ma Dawa, Zhuo-ma Dawa, Ting Zhai, Ting Zhai, Chuan-chuan Liu, Chuan-chuan Liu, Hai-ning Fan, Hai-ning Fan

“…In addition, the effects of PAB on T lymphocyte and collagen synthesis were evaluated after PAB administration in a mouse model.ResultsMetacestode vesicles and germinal cells were successfully cultured, and specific genes were amplified via RT-PCR to identify the protoscoleces, vesicles, and germinal cells as the sources of E. multilocularis. …”
Get full text