Endophytic fungi related to the ash dieback causal agent encode signatures of pathogenicity on European ash by Maryam Rafiqi, Chatchai Kosawang, Jessica A. Peers, Lukas Jelonek, Hélène Yvanne, Mark McMullan, Lene R. Nielsen

“…Phylogenetic analysis of core eukaryotic genes identified H. albidus and H. koreanus as sister species, whilst H. occultus diverged prior to these and H. fraxineus. …”
Get full text

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One o... by Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan, Amany Ahmed Arafa, Eman Shafeek Ibrahim, Doaa Sedky, Amani Abdel nabey Hafez

“…Multiplex PCR was used to detect the methicillin resistance genes in S. aureus to detect the occurrence of MRSA isolates. …”
Get full text

Investigation of Scutellaria Barbata’s immunological mechanism against thyroid cancer using network pharmacology and experimental validation by Gen Ouyang, Yuangui Zhu, Zhehao Ouyang

“…The expression, prognosis, and immunotherapeutic roles of core genes were validated using TCGA databases. Molecular docking demonstrated the binding interactions between core components and targets. …”
Get full text

Isolation, Molecular Characterisation, and Pathogenicity Analysis of a Novel Recombinant ALV‐J Strain Isolated From Chinese Hetian Chickens by Tianyu Lei, Liyun Zhuang, Tingting Dai, Qun Niu, Yinli Bao, Weiming Lin, Cuiqin Huang, Xintian Zheng

“…The proviral genome was amplified using segmented PCR and then spliced together using DNASTAR software. Genome‐wide genes, including gag, pol, gp85, and long terminal repeat (LTR), were compared. …”
Get full text

Neu5Ac Induces Human Dental Pulp Stem Cell Osteo-/Odontoblastic Differentiation by Enhancing MAPK/ERK Pathway Activation by Changzhou Li, Xinghuan Xie, Zhongjun Liu, Jianhua Yang, Daming Zuo, Shuaimei Xu

“…In addition, the expression of genes related to osteo-/odontoblastic differentiation and MAPK signaling at different stages of this differentiation process was analyzed in the presence or absence of Neu5Ac. …”
Get full text

Postharvest wounding treatment influences flavone production and biological activities of the hairy root cultures of Scutellaria baicalensis by Hyeon Ji Yeo, Su Young Shin, Sang Un Park, Chang Ha Park

“…Furthermore, the expression levels of genes involved in root-specific flavone pathways (SbPAL1, SbPAL2, SbPAL3, SbCCL7, SbCHS2, SbCHI, SbFNS2-2, SbCYP82D1.1, and SbF8H) were determined at two time points (control and after 96 h of exposure). …”
Get full text

A Panel of Three MicroRNA Signatures as a Potential Biomarker for CRC Screening Based on Stages and Functional Prediction Using Bioinformatic Analysis by Syarah Syamimi Mohamed, Azmir Ahmad, Nurul Syakima Ab Mutalib, Tengku Ahmad Damitri Al-Astani Tengku Din, Md Salzihan Md Salleh, Andee Dzulkarnaen Zakaria, Zaidi Zakaria

“… (1) Background: MicroRNA (miRNA) has been linked to colorectal cancer (CRC) tumorigenesis due to its post-transcriptional mechanism in targeting cancer-associated genes. Although miRNAs appear to be promising screening biomarkers, functional prediction analysis is required to shed light on their role in CRC tumorigenesis. …”
Get full text

Optimizing the selection of quantitative traits in plant breeding using simulation by Rafael Augusto Vieira, Ana Paula Oliveira Nogueira, Roberto Fritsche-Neto

“…Bayesian methods perform well with fewer genes and in early breeding cycles, while BLUP is more robust for traits with many QTL, and RR-BLUP proves flexible across different conditions. …”
Get full text

Enzymes enabling the biosynthesis of various C20 polyunsaturated fatty acids in a sea urchin Hemicentrotus pulcherrimus by Yingying Peng, Yutaka Haga, Naoki Kabeya

“…Although these fatty acids may partially be derived from their diet, such as macroalgae, the present study on Hemicentrotus pulcherrimus has uncovered multiple genes encoding enzymes involved in long-chain PUFA biosynthesis. …”
Get full text

De Novo Genome Assembly for an Endangered Lemur Using Portable Nanopore Sequencing in Rural Madagascar by Lindsey Hauff, Noa Elosmie Rasoanaivo, Andriamahery Razafindrakoto, Hajanirina Ravelonjanahary, Patricia C. Wright, Rindra Rakotoarivony, Christina M. Bergey

“…The nuclear assembly had 30× average coverage and was comparable in completeness to other primate reference genomes, with a 96.1% BUSCO completeness score for primate‐specific genes. As the first published reference genome for E. rufifrons and the only annotated genome available for the speciose Eulemur genus, this resource will prove vital for conservation genomic studies while our efforts exhibit the potential of this protocol to address research inequalities and build genomic capacity.…”
Get full text

Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi by A. A. Kryukov, A. O. Gorbunova, E. M. Machs, Y. V. Mikhaylova, A. V. Rodionov, P. M. Zhurbenko, A. P. Yurkov

“…In our work, we have successfully used universal primers ITS3 and ITS4 for the sequencing in Illumina MiSeq of the 5.8S rDNA – ITS2 region of the 35S rRNA genes, which contain both a conservative and variable regions. …”
Get full text

Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal by Roshan Pandit, Balkrishna Awal, Sumesh Shreekhanda Shrestha, Govardhan Joshi, Basista Prasad Rijal, Narayan Prasad Parajuli

“…In this study, we aimed to investigate the burden of antimicrobial resistance and occurrence of ESBL genes among clinical isolates of uropathogenic Escherichia coli at a tertiary care teaching hospital of Nepal. …”
Get full text

Newly isolated halotolerant Gordonia terrae S-LD serves as a microbial cell factory for the bioconversion of used soybean oil into polyhydroxybutyrate by Song Xu, Ruiqin Han, Lidan Tao, Zhipeng Zhang, Junfei Gao, Xinyuan Wang, Wei Zhao, Xiaoxia Zhang, Zhiyong Huang

“…In addition, the whole genome was sequenced, and the PHB biosynthetic pathway and quantitative expression of key genes were delineated in the novel isolated strain. …”
Get full text

Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis by Qinzhen Lu, He Ling, Yonghui Lao, Junjie Liu, Wei Su, Zhao Huang

“…Results Our research findings indicate that AOCS is an immune-mediated inflammatory disease, primarily involving immune cells, especially neutrophils. In addition, genes associated with ferroptosis, a form of regulated cell death, are found to be upregulated in the rat model, with non-coding RNAs playing a role in regulatory interactions. …”
Get full text

Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase by Amrit Koirala, Nabilah Ali Alshibli, Bikram K. Das, Volker S. Brözel

“…Putative nitrogen fixers were confirmed by subculturing in liquid nitrogen-free media and PCR amplification of the <i>nifH</i> genes. Streaking of the 432 isolates on nitrogen-rich R2A revealed many cocultures. …”
Get full text

Type 2 Diabetic Mellitus Inhibits Skin Renewal through Inhibiting WNT-Dependent Lgr5+ Hair Follicle Stem Cell Activation in C57BL/6 Mice by Minghui Wang, Shangsheng Yao, Dehua He, Mulan Qahar, Jinqing He, Meifang Yin, Jun Wu, Guang Yang

“…The proliferation of the skin cells was observed by PCNA and K14 staining. The altered genes were screened by RNA sequencing and verified by qRT-PCR. …”
Get full text

Biogeographic Insights Into the Late Miocene Diversification of the Giant Deep‐Ocean Amphipod Eurythenes by Carolina E. González, Johanna N. J. Weston, Reinaldo Rivera, Marcelo Oliva, Rubén Escribano, Osvaldo Ulloa

“…We constructed a time‐calibrated phylogeny using two mitochondrial DNA genes by analyzing publicly available data on 14 species of Eurythenes across a global depth range from 839 to 8081 m. …”
Get full text

Ameliorative Impact of Liraglutide on Chronic Intermittent Hypoxia-Induced Atrial Remodeling by Jun Wang, Yongzheng Liu, Changhui Ma, Yue Zhang, Meng Yuan, Guangping Li

“…Immunohistochemical staining, RT-qPCR, and Western blotting were performed to evaluate the marker levels of AAR and AER and the expression of genes and proteins of the miR-21/PTEN/PI3K/AKT signaling pathway, respectively. …”
Get full text

Rational metabolic engineering of <i>Corynebacterium glutamicum</i> to create a producer of L-valine by M. E. Sheremetieva, K. E. Anufriev, T. M. Khlebodarova, N. A. Kolchanov, A. S. Yanenko

“…Various aspects of L-valine biosynthesis in C. glutamicum are reviewed: process biochemistry, stoichiometry and regulation, enzymes and their corresponding genes, export and import systems, and the relationship of L-valine biosynthesis with central cell metabolism. …”
Get full text

Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia by J. Velasco-Bolaños, A. S. Jaramillo-Jaramillo, N. A. Villa-Arcila, S. Piepers, S. Dufour, A. Ceballos-Márquez

“…Los análisis moleculares se realizaron por PCR multiplex usando cebadores específicos para los genes 16S-23S rRNA del Mycoplasma spp. y de algunas bacterias oportunistas ocasionales en la leche. …”
Get full text