Homology Modeling and Expression of Recombinant NS5-RdRp Based on the Indonesian Local Strain of Dengue Virus for Anti-Dengue Drug Development by Toto Subroto, Ade Rizqi Ridwan Firdaus, Shinta Kusumawardani, Taufik Ramdani Tohari, Theresia Adventina Nababan, Wulan Pertiwi, Muhammad Fadhlillah, Sri Agung Fitri Kusuma, Yaya Rukayadi

“…Then, followed by the extraction and purification of the RdRp amplicon for gene sequencing. The resulting RdRp gene sequences are translated into amino acid sequences, which are then used for protein modeling and the construction of a recombinant plasmid for RdRp protein expression. …”
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Chromosome-level and haplotype-resolved genome assembly of Bougainvillea glabra by Lan Lan, Haiyan Li, Shisong Xu, Yueting Xu, Qingyun Leng, Linbi Zhang, Linqiao Wu, Junmei Yin, Zhiqiang Wu, Junhai Niu

“…By combining the high-confidence different evidence of genome structure annotation pipeline we annotated each haplotype containing ~33,000 predicted genes, of which 86% were functionally annotated by different databases. …”
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Tag7 (PGLYRP1) Can Induce an Emergence of the CD3+CD4+CD25+CD127+ Cells with Antitumor Activity by T. N. Sharapova, E. A. Romanova, L. P. Sashchenko, D. V. Yashin

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Novel Compound Heterozygous PRKN Variants in a Han-Chinese Family with Early-Onset Parkinson’s Disease by Kuan Fan, Pengzhi Hu, Chengyuan Song, Xiong Deng, Jie Wen, Yiming Liu, Hao Deng

“…Genetic factors are thought to play an important role in the pathogenesis of Parkinson’s disease (PD), particularly early-onset PD. The PRKN gene is the primary disease-causing gene for early-onset PD. …”
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Combined pyrotinib and fulvestrant for hormone receptor‐positive and HER2‐positive metastatic breast cancer: A multicenter, single‐arm, phase II trial by Jianli Zhao, Yunfang Yu, Wei Ren, Linxiaoxiao Ding, Yongjian Chen, Peng Yuan, Jian Yue, Yaping Yang, Guorong Zou, Tao Chen, Jie Chai, Li Zhang, Wenjing Wu, Yinduo Zeng, Xiujuan Gui, Yangyang Cai, Simin Luo, Zhongyu Yuan, Kang Zhang, Herui Yao, Ying Wang

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Different functions of PHF10 isoforms – subunits of the PBAF chromatin remodeling complex by A. A. Sheynov, V. V. Tatarskiy, A. M. Azieva, S. G. Georgieva, N. V. Soshnikova

“…Chromatin remodelling multiprotein complexes play an important role in regulation of gene expression in embryogenesis and in the adult organism. …”
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Phytoalexin deficient 4 is associated with the lesion mimic trait in watermelon clalm mutant (Citrullus lanatus) by Jiale Shi, Congji Yang, Yuanyuan Qin, Qingqing Liu, Shengqi Hua, Defeng Wu, Wei Dong

“…As revealed by analysis of the lesion mimic mutant clalm, this trait is controlled by a single dominant gene. Whole genome resequencing–bulked segregant analysis demonstrated that this gene is located on chromosome 4 from 3,760,000 bp to 7,440,000 bp, a region corresponding to a physical distance of 3.68 Mb encompassing approximately 72 annotated genes. …”
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Transcription factor TCF4: structure, function, and associated diseases by R. R. Savchenko, N. A. Skryabin

“…Our understanding of human genes - particularly their structure, functions, and regulatory mechanisms - is still limited. …”
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Molecular Phylogenetic Relationships Based on Mitogenomes of Spider: Insights Into Evolution and Adaptation to Extreme Environments by Rongxiang Zhang, Niyan Xiang, Xiaoman Gao, Guiyu Zhang, Tian Lu, Tao Yuan

“…Selection analysis detected positive selection signals in the ATP8 gene in Desis jiaxiangi. The ND5 gene is a convergent evolutionary gene between D. jiaxiangi and A. aquatica. …”
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The Association of the GABRP Polymorphisms with Systemic Lupus Erythematosus by Hun-Soo Kim, Eun-Heui Jin, Ji-Su Mo, Hyeok Shim, Shin-Seok Lee, Soo-Cheon Chae

“…This study was aimed to validate whether the polymorphisms in the GABRP gene are associated with the susceptibility to systematic lupus erythematosus (SLE). …”
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Combined Effects of 2-Methoxyestradiol (Hypoxia-Inducible Factor 1α Inhibitor) and Dasatinib (A Second-Generation Tyrosine Kinase Inhibitor) on Chronic Myelocytic Leukemia Cells by Yuexian Zhang, Heng Chen, Yunfeng Shen, Xin Zhou

“…The activation of this gene mostly marks a poor outlook for cancer patients. …”
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Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of Candida albicans and Inflammatory Host Response In Vitro by Emily Chen, Bruna Benso, Dalia Seleem, Luiz Eduardo Nunes Ferreira, Silvana Pasetto, Vanessa Pardi, Ramiro Mendonça Murata

“…In conclusion, curcumin exhibited antifungal activity against C. albicans and modulated the proteolytic enzyme activities without downregulating the gene expression. In host inflammatory response, curcumin downregulated IL-1β and IL-1α gene expression.…”
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Viral and Nonviral Engineering of Natural Killer Cells as Emerging Adoptive Cancer Immunotherapies by Sandro Matosevic

“…However, NK cells are notoriously adverse to endogenous gene uptake, resulting in low gene uptake and transgene expression with many vector systems. …”
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The Immune Landscape of Hepatitis B Virus-Related Acute Liver Failure by Integration Analysis by Jiao Gong, Yaqiong Chen, Jing Cao, Yang Wang, Jiahao Chen, Danyang Li, Liuping Sha, Xinhua Li, Yutian Chong, Bo Hu

“…The enrichment scores of cell death gene sets from Kyoto Encyclopedia of Genes and Genomes (KEGG) were calculated by gene set variation analysis method, and a protein-protein interaction (PPI) network was constructed using Cytoscape. …”
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Multi-INTACT: integrative analysis of the genome, transcriptome, and proteome identifies causal mechanisms of complex traits by Jeffrey Okamoto, Xianyong Yin, Brady Ryan, Joshua Chiou, Francesca Luca, Roger Pique-Regi, Hae Kyung Im, Jean Morrison, Charles Burant, Eric B. Fauman, Markku Laakso, Michael Boehnke, Xiaoquan Wen

“…Abstract We present multi-integration of transcriptome-wide association studies and colocalization (Multi-INTACT), an algorithm that models multiple “gene products” (e.g., encoded RNA transcript and protein levels) to implicate causal genes and relevant gene products. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. …”
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Putative regulatory functions of SNPs associated with bronchial asthma, arterial hypertension and their comorbid phenotype by I. A. Goncharova, E. Yu. Bragina, I. Zh. Zhalsanova, M. B. Freidin, M. S. Nazarenko

“…These SNPs can affect binding affinity of transcription factors of the Pou and Klf4 families, as well as the expression levels of the TLR4 and SERPINA1 genes. The rs11065987 allele A of the ATXN2/BRAP genes, the rs11669386 A allele of the CARD8 gene, the rs2284033 allele G of the IL2RB gene, and the rs11191582 allele G of the NT5C2 gene were associated with the risk of AH. …”
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Characterization of Staphylococcus aureus Strains Isolated from Veterinary Hospital by Ariel E. Stella, Thaís F. Lima, Cecília N. Moreira, Eric M. N. De Paula

“…S. aureus was identified in 7.6% (21/276) of the samples collected, and of the 21 strains isolated, 4 (19.0%) carried the mecA gene. MRSA are all strains of S. aureus that express the mecA gene. …”
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Functional Expression Study of Igf2 Antisense Transcript in Mouse by Carolina Duart-Garcia, Martin H. Braunschweig

“…Insulin-like growth factor antisense gene (Igf2as) expression was investigated in different mouse tissues during development, in differentiating C2C12 cells and in a ΔDMR1-U2 knockout mouse model. …”
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Homozygous CALR Mutation in Primary Myelofibrosis and Its Effect on Disease Phenotype: A Case Report and Review of the Literature by Qurratulain Rizvi, Uzma Zaidi, Saba Shahid, Shariq Ahmed, Tahir Shamsi

“…Somatic mutations in CALR gene have been reported in 60%–88% of patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF) who are negative for JAK2 and MPL mutations. …”
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