THE BALANCE OF PRIVATE AND PUBLIC INTERESTS IN CRIMINAL PROCEEDINGS: JUDICIAL POLITY by SMIRNOV Alexander Vitalievich

“…Methods: structural-functional analysis, comparative-historical, institutional, typological, evolutionary, genetic, and formal-legal methods. …”
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Impact of obesity on proteomic profiles of follicular fluid-derived small extracellular vesicles: A comparison between PCOS and non-PCOS women by Qiyuan Chang, Senlan Wang, Qingyun Mai, Canquan Zhou

“…Additionally, differential functional analysis and PPI analysis indicated significant pathway and key proteins alterations in the PCOS group related to inflammation, while non-PCOS women demonstrated metabolic reprogramming and anti-inflammatory responses, suggesting a differential response to obesity that may preserve oocyte quality. …”
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Spatial transcriptomic characteristics of gastric cancer in young and the expression and role of TMEM176B in gastric cancer cells by Zhenhua Jiang, Yongfeng Wang, Chi Zhang, Shuangyin Han

“…Based on the results, TMEM176B was selected for functional analysis. Western blotting was used to assess TMEM176B expression in normal gastric cells and cancerous cells. shRNA-mediated TMEM176B knockdown in cancer cells was used for phenotypic analysis, proliferation assays, and apoptosis experiments. …”
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The UFMylation pathway is impaired in Alzheimer’s disease by Tingxiang Yan, Michael G. Heckman, Emily C. Craver, Chia-Chen Liu, Bailey D. Rawlinson, Xue Wang, Melissa E. Murray, Dennis W. Dickson, Nilufer Ertekin-Taner, Zhenkun Lou, Guojun Bu, Wolfdieter Springer, Fabienne C. Fiesel

“…In addition, we found that the levels of conjugated UFM1 were negatively correlated with soluble levels of the deUFMylation enzyme UFSP2. Functional analysis of UFM1 and/or UFSP2 knockout neurons revealed that the DNA damage response as well as the unfolded protein response are perturbed by changes in neuronal UFM1 signaling. …”
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Animal board invited review: The need for, and the path towards, a functional understanding of the farmed insect microbiome by N.A. Sibinga, E. Werner, D. Tegtmeier, J. De Smet

“…This review therefore aims to critically assess the state of the art with regard to functional analysis of the farmed insect microbiome and how available experimental methods can be best applied to identify links between microbial functions and insect physiology and improve the efficiency and sustainability of the farmed insect industry.…”
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Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance by Senhui Wei, Ying Li, Jing Zhou, Yongming Xia

“…Methylation profiling further supported the idea that promoter hypomethylation of MAP3K1, MAP3K4, MAP3K7, MAP3K8, MAP3K9, and MAP3K10 genes was associated with their up-regulation. Single-cell functional analysis elucidated the involvement of MAP3K genes in shaping the tumor microenvironment. miRNA-mRNA network analysis revealed intricate regulatory mechanisms, with hsa-mir-200b-3p emerging as a key regulator. …”
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Technology and optimization of preparation and regeneration of protoplasts from rice sheath blight fungus Rhizoctonia solani by KONG Dandan, QUE Yawei, YAN Xia, LI Ya, CHEN Weiliang, WANG Zhengyi

“…These characteristics of R. solani may make it difficult to perform genetic transformation and functional analysis of genes. Production of large amount of protoplasts from this fungus is a prerequisite for the studies of molecular genetics, such as protoplast fusion and fungal transformation. …”
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Co-modulation of a circular form of PCDH11Y during neuroendocrine differentiation of prostate cancer by Giovanni Pecoraro, Ilaria Leone, Silvia Nuzzo, Santiago Negueruela, Giovanni Smaldone, Lorena Buono

“…After that, validation and functional analysis by RNA-interference was applied to a selected circRNA to explore its role during NED. …”
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Expression pattern and function analyses of the MADS thranscription factor genes in wheat (Triticum aestivum L.) under phosphorus-starvation condition by Shu-ya SHI, Fei-fei ZHANG, Si GAO, Kai XIAO

“…TaMADS51, one of the upregulated genes by Pi deprivation, was subjected to the functional analysis in mediating plant tolerance to the Pi-starvation stress. …”
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Streptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes by Haruno Yoshida, Mieko Goto, Yuzo Tsuyuki, Jae-Seok Kim, Takashi Takahashi

“…Gene ontology enrichment analysis showed most of the gene expressions were downregulated. DEG functional analysis showed the downregulated DEGs belonging to energy production and conversion/carbohydrate transport and metabolism/amino acid transport and metabolism/nucleotide transport and metabolism, with the upregulated DEGs belonging to transcription. qRT-PCR assays for downregulated/upregulated expressions of four genes (pgk–slo/opuAA–kdpB) validated transcriptomic results. …”
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A metagenome-wide association study of gut microbiota in hepatitis B virus-related cirrhosis in northwest China by Meirong Feng, Yan Chai, Jinna Li, Qi Wang, Dekui Zhang

“…Species-level analysis identified distinct enrichment of Prevotella copri, Bacteroides vulgatus, Escherichia coli, Fusobacterium nucleatum, and Veillonella spp. in HBV-LC. Functional analysis revealed 482 metabolic pathways. HBV-LC showed enhanced lipid, amino acid, and nucleotide metabolism, menaquinol biosynthesis, and anaerobic energy metabolism, but reduced acetate/lactate production, lactose/galactose degradation, and peptidoglycan biosynthesis. …”
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Unraveling the molecular mechanisms of DNA capture by the Com pilus in naturally transformable monoderm bacteria by Jérémy Mom, Odile Valette, Laetitia Pieulle, Vladimir Pelicic

“…Here, we address this question for the Com pilus, a widespread T4F mediating DNA capture in competent monoderm species. Our functional analysis, performed in Streptococcus sanguinis, was guided by a complete structural model of the Com pilus. …”
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Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription by Lipeng Pei, Yan Zhu, Wenpeng Li, Bin Xu, Jingli Sun

“…RBPJ could bind to the MCM4 promoter to suppress the luciferase activity. Further functional analysis revealed that the functions of KAT2B in UCEC cell proliferation, DNA damage and apoptosis were mediated by MCM4, and Celastrol enhanced RBPJ acetylation and reduced MCM4 expression. …”
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A novel founder variant in BEST1 gene causing autosomal recessive bestrophinopathy by Nagham Maher Elbagoury, Caroline Atef Tawfik, Asmaa Fawzy Abdel-Aleem, Heba Mahmoud Fathy, Dina Nabil Baddar, Mona Lotfi Essawi

“…The pathogenicity of the variants was tested using different in silico functional analysis tools. Results The clinical examination and investigations confirmed the ARB phenotype. …”
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The Significance and Diagnostic Potential of CEA and FIB in Colorectal Cancer by Lin Zhu, Jie Feng, Xu Zhang, Xuemei Wei, Cuiling Ming, Yanhong Gao

“…Protein‐protein interaction network analysis showed that the fibrinogen alpha (FGA) gene had the strongest correlation with albumin (ALB), alpha‐2‐Heremans Schmid glycoprotein (AHSG), and serpin peptidase inhibitor, clade D, member 1 (SERPIND1). Gene Ontology functional analysis in CRC showed that FGA and related genes were enriched in biological processes including biosynthesis of ribonucleoprotein complex and non‐coding ribonucleic acid metabolic process; in cellular components, they were primarily enriched in pre‐ribosomes; and in molecular functions, they were mainly enriched in binding of unfolded protein. …”
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Comprehensive bioinformatics analysis identifies hub genes associated with immune cell infiltration in early-onset schizophrenia by Shasha Wu, Tailian Xue, Yilin Li, Weikang Chen, Yan Ren

“…Subsequently, a PPI network was constructed to select the top 10 potential hub genes, and functional analysis was performed by GeneMANIA, resulting in the identification of four immune-related genes. …”
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STay tunEd: mutational analysis of the HvSTE1 gene in barley provides insight into the balance between semi-dwarfism and maintenance of grain size in brassinosteroid biosynthesis-d... by Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka

“…The aim of this study was to identify and functionally analyze the STE1 gene in barley (Hordeum vulgare) which is an important cereal crop. The functional analysis was carried out with the application of TILLING (Targeting Induced Local Lesions IN Genomes) approach. …”
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Genome-wide identification of PP2A gene family in Camellia sinensis reveals the potential role of CsPP2A-TON2/FASS1 in abiotic stress by Surjit Bhattacharjee, Abhirup Paul, Aradhana Jana, G. Meher Unnati, Deepak R., Ye Miao, Honglin Lu, Guoxin Shen, Neelam Mishra

“…Conclusion This study offers potential goals for further in-depth investigation and functional analysis, alongside enhancing our understanding of the regulatory network of PP2A genes in C. sinensis.…”
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Clinical and functional characterization of a novel TNFRSF9 variant causing immune dysregulation with predisposition to EBV-driven lymphomagenesis by Peiwei Zhao, Peiwei Zhao, Kailan Chen, Li Yang, Chunhui Wan, Lei Zhang, Sukun Luo, Xuelian He, Xuelian He

“…In vitro functional study was performed by western blot, flow cytometry assays and luciferase assays.ResultsGenetic analysis identified a novel missense variant in the TNFRSF9 gene (NM_001561.5: c.359G>C, p.C120S). Functional analysis in vitro demonstrated that this variant decreased the expression of TNFRSF9 both mRNA and protein levels. …”
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Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration by Jing Chen, Tu Lu, Chen Chen, Wenbin Zheng, Lin Lu, Naiyang Li

“…Results We identified hub ferroDEGs that include six genes (ANXA1, DKK1, CD44, VIM, TGFB2, DUSP1). Functional analysis of those hub ferroDEGs was found to be correlated with leukocyte migration and chemotaxis, macrophage migration, and gliogenesis. …”
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