Long non-coding RNA MIR22HG impedes the progression of anaplastic thyroid carcinoma via targeting miR-141-3p/PTEN/AKT axis by Junyu Cao, Peng Jiang, Songliang Jiang, Yuhan Zhao, Xin Jiang, Chuancheng Zhou, Jing Li, Kunxian Yang

“…More importantly, decreased expression of MIR22HG was found to be correlated with poor prognosis of ATC patients. Functional analysis showed that overexpression of MIR22HG attenuated the proliferation and metastasis of ATC both in vitro and in vivo. …”
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Unveiling the versatility of the thioredoxin framework: Insights from the structural examination of Francisella tularensis DsbA1 by Stephanie Penning, Yaoqin Hong, Taylor Cunliffe, Lilian Hor, Makrina Totsika, Jason J. Paxman, Begoña Heras

“…FtDsbA1’s redox properties are comparable to other DsbA enzymes, however, consistent with its atypical structure, functional analysis reveals FtDsbA1 has a high degree of substrate specificity suggesting a specialised role within F. tularensis’ oxidative folding pathway. …”
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MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line by A. V. Korenevsky, A. D. Shcherbitskaia, M. E. Berezkina, K. L. Markova, E. P. Alexandrova, O. A. Balabas, S. A. Selkov, D. I. Sokolov

“…With automated methods of functional analysis  applied, it has been  shown  that  the  largest  protein groups  are  hypothetical proteins, proteins with  unknown functions, and  domains. …”
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GHMP gene family: identification, evolutionary and expression analysis under various exogenous hormones and abiotic stress in tomato by Haitao Yang, Xin Bai, Tayeb Muhammad, Tao Yang, Chunping Jia, Baike Wang, Qinghui Yu, Juan Wang

“…However, the identification and functional analysis of GHMP family members in tomato have not been carried out yet. …”
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Systematic Review of Proteomics in Age-Related Macular Degeneration and Pathway Analysis of Significant Protein Changes by Olympia Sideri, MD, MSc, Victor Correa, MD, Nikolaos Ziakas, MD, PhD, Ioannis Tsinopoulos, MD, PhD, Joan W. Miller, MD, PhD, Demetrios G. Vavvas, MD, PhD

“…Although many proteins differed significantly between AMD and control groups, most exhibited less than a 2-FC. Functional analysis of proteins with ≥|2|-FCs (identified by unbiased proteomics in multiple biofluids) highlighted lipid metabolism and protease regulation pathways as central to both dry and wet AMD. …”
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Rhizosphere microbiome metagenomics in PGPR-mediated alleviation of combined stress from polypropylene microplastics and Cd in hybrid Pennisetum by Si-Yu Zhao, Yue-Liang Meng, Zi-Han Yang, B. Larry Li, Yu-Ying Li, Hui Han, Ling Liu, Peng-Fei Duan, Zhao-Jin Chen

“…The metagenomic studies indicated that the cocontamination of PP and Cd, along with PGPR inoculation, altered the composition of the rhizospheric bacterial community, leading to changes in microbial diversity indices and the composition of dominant groups such as Pseudomonadota, Actinomycetota, and Acidobacteriota. The functional analysis revealed that the main functional groups involved glucose metabolism, energy metabolism, signal transduction, and nucleotide metabolism. …”
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Integrative analysis of taurine metabolism-related genes prognostic signature with immunotherapy and identification of ABCB1 and GORASP1 as key genes in nasopharyngeal carcinoma by Zhang Feng, Yuhang Yang, Wenqi Luo, Jinqing Li, Zhenlian Xie, Long Zuo, Meijiao Duan, Dongzhi Zuo, Ruwei MO, Xuejing Tang, Shijiang Yi, Xiaosong He, Fangxian Liu, Ning Ma, Feng He

“…Our prognostic model was based on three pivotal TMRGs (ABCB1, GORASP1, and EZH2). Functional analysis revealed a strong association between TMRGs and miRNAs in cancer. …”
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Impact of alkaline and deep eutectic solvent extraction on rapeseed protein isolates characteristics and in vitro digestibility by Branislava Đermanović, Aleksandar Marić, Marijana Sakač, Damjana Tomić, Danka Dragojlović, Ljiljana Popović, Bojana Šarić, Pavle Jovanov

“…DES-based isolates exhibited a more balanced amino acid profile, particularly for sulphur-containing amino acids, making them more valuable protein sources compared to alkaline-extracted proteins. Techno-functional analysis favoured ALK12 for products requiring high water and oil absorption capacity, and foam stability, while DES1, despite lower absorption and foaming capacity, offered good solubility making it suitable for a wide range of applications. …”
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Effect of microbial diversity and their functions on soil nutrient cycling in the rhizosphere zone of Dahongpao mother tree and cutting Dahongpao by Xiaoli Jia, Lei Hong, Lei Hong, Yulin Wang, Qi Zhang, Yuhua Wang, Miao Jia, Yangxin Luo, Tingting Wang, Jianghua Ye, Haibin Wang, Haibin Wang

“…There were eight characteristic microorganisms that significantly differed between MD and PD rhizosphere soils, and the results of functional analysis showed that MD rhizosphere soil microorganisms had higher carbon, nitrogen, and phosphorus biotransformation capacity, were more conducive to the accumulation and release of nutrients in the soil, and were more conducive to the promotion of tea tree growth. …”
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A novel mitochondrial quality regulation gene signature for anticipating prognosis, TME, and therapeutic response in LUAD by multi-omics analysis and experimental verification by Lijun Zeng, Sixuan Wu, Zhimin Li, Yuanbin Tang, Yeru Tan, Renji Liang, Yuehua Li

“…High-risk patients experienced significantly lower survival rates. Functional analysis linked these MQRGs to alpha-linolenic acid metabolism pathways. …”
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Over-expression analysis of TaDWF4˗4B in mutant wheat lines identifies a candidate regulator of heat stress tolerance by Liaqat Shah, Muhammad Saeed, Muhammad Ibrahim, Waqas Ahmad, Abdul Rahman Umar, Ayan Sohail, Hongqi Si

“…Among them, TaDWF4˗4B showed highest expression under heat stress condition and selected for further functional analysis. Overexpression of TaDWF4˗4B in the wheat line ESWYT-4 enhanced heat tolerance. …”
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Cyclin D2 – a potential biomarker in uterine corpus endometrial carcinoma through methylation chip and bioinformatic analysis by Lizhen Wang, Lizhen Wang, Wu Qu, Zhifu Yang

“…CCND2 was selected for further functional analysis. IHC results revealed that the positive expression of CCND2 in UCEC was significantly lower than in normal endometrial tissue (P < 0.05). …”
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ATP6AP1 drives pyroptosis-mediated immune evasion in hepatocellular carcinoma: a machine learning-guided therapeutic target by Lei Tang, Xiyue Wang, Zhengzheng Xia, Jiayu Yan, Shanshan Lin

“…Multiple machine learning techniques, including GBM (gradient boosting machine), XGBoost (extreme gradient boosting machine), SVM (support vector machine), LASSO (least absolute shrinkage and selection operator) and random forest, as well as functional analysis, were used to systematically investigate the role of ATP6AP1 in HCC. …”
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Transcriptomic insights into the mechanism of action of telomere-related biomarkers in rheumatoid arthritis by Lijuan Feng, Kaiyong Bai, Limeng He, Hao Wang, Wei Zhang

“…Subsequently, the potential mechanisms of these biomarkers in RA were further explored using gene set enrichment analysis (GSEA), subcellular localization, chromosome localization, immune infiltration, functional analysis, molecular regulatory networks, drug prediction, and molecular docking. …”
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Combined Functional and Structural Imaging of White Matter Reveals Brain Connectivity Alterations in Fibromyalgia Patients by Gao Z, Xie X, Liu F, Xu T, Zhang N, Zhang X, Li Y, Kong Y, Lv D, Wu T

“…Some regions showing structural changes also showed changes in resting-state functional activation. Functional analysis showed that FMS patients have reduced ALFF values in the left CST, left ML, right PCR, right cingulate/hippocampus, and right FX/ST. …”
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HLA-Cw*0102-restricted HIV-1 p24 epitope variants can modulate the binding of the inhibitory KIR2DL2 receptor and primary NK cell function. by Lena Fadda, Christian Körner, Swati Kumar, Nienke H van Teijlingen, Alicja Piechocka-Trocha, Mary Carrington, Marcus Altfeld

“…However, only one peptide (p24 Gag₂₀₉₋₂₁₈ AAEWDRLHPV) allowed for binding of KIR2DL2. Notably, functional analysis showed a significant inhibition of KIR2DL2⁺ NK cells in the presence of p24 Gag₂₀₉₋₂₁₈-pulsed T2 cells, while degranulation of KIR2DL2⁻ NK cells was not affected. …”
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Modern Pushkin Studies at the International Congress of Teachers and Lecturers of Russian Language and Russian Literature, Dedicated to the 225th Anniversary of the Birth of A.S. P... by Tatiana V. Kortava, Larisa A. Dunaeva, Victoria G. Moiseeva

“…The methodological foundation of the article is the interdisciplinary approach and the structural and functional analysis of a set of problems associated with the study and teaching of Pushkin’s work in the modern cultural and historical context. …”
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Identification of Genes for Improving Cold Sensitivity in Nerve-Damaged Rats Via Lumbar Sympathectomy Using Poly(A)-seq by Xi Meng, Hanrui Fan, Ping Xu, Lei Yang, Yong Fei, Wenping Zhang

“…We identified 174 DEGs in the gene expression profile of lumbar sympathectomized SNI rats, including 69 upregulated and 105 downregulated genes, compared with SNI model rats. Functional analysis of the DEGs revealed that the most significantly enriched pathways included immune-related pathways and cellular molecular components, which mediate neuroinflammation, central sensitization, and chronic pain. …”
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De novo, high-quality assembly and annotation of the halophyte grass Aeluropus littoralis draft genome and identification of A20/AN1 zinc finger protein family by Walid Ben Romdhane, Rania Ben Saad, Emmanuel Guiderdoni, Ahmed Abdelrahim mohamed Ali, Mohamed Tarroum, Abdullah Al-Doss, Afif Hassairi

“…Here, we identified the complete set of members of this family in the Aeluropus littoralis genome, thereby laying the foundation for their future functional analysis in cereal crops. In addition, the expression patterns of four novel genes from this family were analyzed by qPCR. …”
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Differential Diagnosis of Hurler and Hurler–Scheie Syndromes: Clinical Case by Nataliya V. Buchinskaya, Anastasiya O. Vechkasova, Ekaterina E. Shishunova, Yuliya S. Kravtsova, Nato D. Vashakmadze, Nataliya V. Zhurkova, Mikhail M. Kostik

“…Molecular genetic testing has revealed the presence of the frequent pathogenic variant c.208C>T (Gln70Ter) in the IDUA gene associated with severe disease course and nucleotide variant c.1524G>C (p.Glu508Asp) with undefined clinical significance, thus, additional examination is necessary. Functional analysis of the novel variant is required to establish its role in protein function. …”
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