miR-32-5p suppresses the progression of hepatocellular carcinoma by regulating the GSK3β/NF-κB signaling by Wang Guangzhi, Yang Qianqian, Han Yaqi, Zhang Yunlong, Pan Wei, Ma Zhongliang, Tian Hui, Qu Xudong

“…In this study, qRT-PCR is utilized to precisely determine the downregulated expression levels of miR-32-5p in HCC. Subsequently, functional analysis reveals the suppressive role of miR-32-5p in modulating the proliferative and migratory capabilities of HCC cells. …”
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A Comparative Transcriptomic Analysis of miRNAs and Their Target Genes During the Formation of Melanin in <i>Apis mellifera</i> by Xiangjie Zhu, Yuanmingyue Tian, Mingjie Cao, Chenyu Zhu, Jiaqi Shang, Jiaqi Sun, Yiming Liu, Bingfeng Zhou, Shujing Zhou, Xinjian Xu

“…A total of 171, 94, and 19 DEmiRNAs were identified in these groups, respectively, which regulate 1481, 690, and 182 differentially expressed target mRNAs (target DEmRNAs). The functional analysis of target DEmRNAs indicated that DEmiRNAs might regulate the formation of capped brood melanin in honeybees by activating expression changes in key genes in signaling pathways, such as the Wnt signaling pathway, melanogenesis, and the Toll and Imd signaling pathway, through activating miR-315-x, miR-8, <i>ple</i>, <i>yellow</i> family genes, <i>wnt1</i>, etc. …”
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Transcriptomic analysis reveals the function of m6A regulators in aged cochlea by Yanbing Lai, Bo Su, Xiaodi Wang, Chenghui Zeng, Hanqi Chu, Liangqiang Zhou, Dan Bing

“…Differential expression analysis and downstream functional analysis was performed to identify m6A regulators. …”
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Propofol orchestrates long non-coding RNAs in MCF7 cells, unraveling new avenues for breast cancer intervention by Cigir Biray Avci, Tuba Gokdogan Edgunlu, Tugba Suzek, Neslihan Pinar Ozates, Bakiye Goker Bagca, Aysegul Demirtas Bilgic, Cilem Ozdemir, Bakiye Ugur

“…The study used qRT-PCR to analyze cancer-related lncRNA expressions following propofol treatment; this was supported by RNA-RNA interaction predictions and in silico functional analysis using selected datasets and the R cluster Profiler GSEABase package. …”
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Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping by Bianhong Zhang, Lina Tang, Zhicheng Chen, Xiaoyan Chen, Lindong You, Ruixin Pan, Ting Chen, Yifei Liu, Wenxiong Lin, Wenxiong Lin, Jinwen Huang

“…Lime treatments (L, L+B) promoted nitrification potential in rhizosphere soil, whereas biochar application (B, L+B) resulted in elevated nitrate nitrogen content. Microbial functional analysis indicated that lime (L, L+B) enhanced nitrification, while biochar (B, L+B) fostered dissimilatory nitrate reduction, thereby improving nitrogen retention. …”
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Genome-wide identification and characterization of the bZIP family in the Mangrove Plant Kandelia obovata and its role in response to stress by Yuchong Yi, Yuting Qiu, Hongyao Hu, Dandan Qin, Hechen Huang, Taiping Chen, Wenqi Zha, Yingjia Shen

“…Conclusion This study provides a comprehensive characterization and functional analysis of the bZIP gene family in K. obovata, offering new insights into their roles in plant development and environmental adaptation. …”
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Stunting is associated with persistent and transferable alterations in the gut microbiome by Joshua O. Amimo, C. N. Kunyanga, S. A. Raev, M. Kick, H. Micheal, L. J. Saif, Anastasia N. Vlasova

“…In contrast, we noted the presence of more genes associated with antimicrobial-resistance (AMR) bacteria in stunted toddlers, possibly due to early-life antibiotic treatments. Of interest, functional analysis showed that CAZymes associated with carbohydrate biosynthesis, and a few metabolic pathways related to protein/amino acid, carbohydrate and fat catabolism were enriched in stunted toddlers. …”
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From gut microbiota to host genes: A dual-regulatory pathway driving body weight variation in dagu chicken (Gallus gallus domesticus) by Xiaohan Li, Xueru Han, Huan Yan, Hongyan Zhu, Hongcai Wang, Desheng Li, Yumin Tian, Yuhong Su

“…A conserved functional concordance was observed between regionally predominant differential microbiota and the physiological specialization of corresponding intestinal niches. Functional analysis revealed that the high-body-weight group demonstrated superior capabilities in microbial biosynthesis, whereas the low-body-weight group exhibited enhanced microbial metabolic activity. …”
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Systemic stem cell treatment rescues injured motoneurons by reducing L-selectin expression on leukocytes by Tamás Bellák, Zoltán Fekécs, Dénes Török, Rebeka Kristóf, Omoikhoje Esezoobo, Annamária Marton, Csaba Vizler, Antal Nógrádi, Krisztián Pajer

“…Treated animals received various doses of NE-4C stem cells intravenously and the survival and regeneration of the affected motoneurons was checked by morphological and functional analysis. The molecular changes within the treated cord were followed by the ELISA Proteome Profiler rat cytokine array and qPCR analysis. …”
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Ornithine enhances common bean growth and defense against white mold disease via interfering with SsOAH and diminishing the biosynthesis of oxalic acid in Sclerotinia sclerotiorum by Yasser Nehela, Yasser S. A. Mazrou, Nehad A. EL_Gammal, Osama Atallah, Abdelrazek S. Abdelrhim, Sumit Kumar, Temoor Ahmed, Temoor Ahmed, Qurban Ali, Abeer H. Makhlouf, Warda A. M. Hussain

“…Moreover, in silico analysis showed that the genome of S. sclerotiorum possesses a putative oxaloacetate acetylhydrolase (SsOAH) protein that is highly similar in its functional analysis, conserved domains, and topology with OAH from Aspergillus fijiensis (AfOAH) and Penicillium lagena (PlOAH). …”
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Atypical cancer risk profile in carriers of Italian founder BRCA1 variant p.His1673del: Implications for classification and clinical management by Giovanni Innella, Cristina Fortuno, Laura Caleca, Bing‐Jian Feng, Courtney Carroll, Michael T. Parsons, Sara Miccoli, Marco Montagna, Daniele Calistri, Laura Cortesi, Barbara Pasini, Siranoush Manoukian, Daniela Giachino, Laura Matricardi, Maria Cristina Foti, Valentina Zampiga, Claudia Piombino, Elena Barbieri, Francesca Vignolo Lutati, Jacopo Azzolini, Rita Danesi, Valentina Arcangeli, Sandrine M. Caputo, Nadia Boutry‐Kryza, Vincent Goussot, Susan Hiraki, Marcy Richardson, Hereditary Breast/Ovarian Cancer IOV network (HBOC IOVnet), Simona Ferrari, Paolo Radice, Amanda B. Spurdle, Daniela Turchetti

“…Likelihood‐ratio in favor of pathogenicity was 98898642.82 under assumption of standard BRCA1 breast and ovarian penetrance, and 104240832.84 after excluding breast cancer diagnoses (based on penetrance results). Functional analysis demonstrated that the variant abrogates the BRCA1‐ABRAXAS1 binding, supporting the PS3 code assignment within the ACMG/AMP rule‐based model. …”
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A prognostic signature of Glutathione metabolism-associated long non-coding RNAs for lung adenocarcinoma with immune microenvironment insights by Junxi Hu, Junxi Hu, Shuyu Tian, Shuyu Tian, Qingwen Liu, Qingwen Liu, Jiaqi Hou, Jiaqi Hou, Jun wu, Jun wu, Xiaolin Wang, Xiaolin Wang, Yusheng Shu, Yusheng Shu

“…A prognostic model was built with Cox and Least Absolute Shrinkage and Selection Operator (LASSO) methods and validated by Kaplan-Meier analysis, Receiver Operating Characteristic (ROC) curves, and Principal Component Analysis (PCA). Functional analysis revealed immune infiltration and drug sensitivity differences. …”
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Identification of key genes related to growth of largemouth bass (Micropterus salmoides) based on comprehensive transcriptome analysis by Dayan Hu, Jieliang Jian, Jinpeng Zhang, Xiaojun Xu, Shu Wang, Cuiping Gong, Yuanqin Zhang, Pengcan Zhu, Zhimin Gu, Wenzhi Guan

“…Differential expression between groups was analyzed using DESeq2. GO functional analysis and KEGG pathway analysis were then performed for differentially expressed genes. …”
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Genomic Characterization and Safety Evaluation of <i>Enterococcus lactis</i> RB10 Isolated from Goat Feces by Nattarika Chaichana, Sirikan Suwannasin, Jirasa Boonsan, Thunchanok Yaikhan, Chollachai Klaysubun, Kamonnut Singkhamanan, Monwadee Wonglapsuwan, Rattanaruji Pomwised, Siriwimon Konglue, Rusneeta Chema, Manaschanan Saivaew, Komwit Surachat

“…<b>Methods:</b> In this study, <i>Enterococcus lactis</i> RB10, isolated from goat feces, was subjected to comprehensive genomic and functional analysis to assess its safety and potential as a probiotic strain. …”
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Genome Selection and Genome-Wide Association Analyses for Litter Size Traits in Large White Pigs by Yifeng Hong, Xiaoyan He, Dan Wu, Jian Ye, Yuxing Zhang, Zhenfang Wu, Cheng Tan

“…Furthermore, a single-step genome-wide association study (ssGWAS) integrated genomic information and pedigree-based relationship matrices to screen for significant SNPs associated with litter size traits across the genome. Functional analysis of key candidate genes was subsequently conducted based on ssGWAS results. (3) Results: Heritabilities for litter traits ranged from 0.01 to 0.06. ssGBLUP improved genomic prediction accuracy by 6.38–13.33% over BLUP. …”
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HSPA2 influences the differentiation and production of immunomodulatory mediators in human immortalized epidermal keratinocyte lines by Agnieszka Gogler, Agata Małgorzata Wilk, Damian Robert Sojka, Małgorzata Adamiec-Organiściok, Natalia Matysiak, Daria Kania, Klaudia Wiecha, Ewa Małusecka, Alexander Jorge Cortez, Dawid Zamojski, Michał Marczyk, Agnieszka Maria Mazurek, Sylwia Oziębło, Dorota Scieglinska

“…Transcriptomic analysis revealed that the total loss of HSPA2 affected the expression of genes related to keratinocyte differentiation and interleukin- and interferon-mediated signaling. The functional analysis confirmed bidirectional changes associated with the loss of HSPA2. …”
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Effects of aging on otolith morphology and functions in mice by Keita Ueda, Takao Imai, Taeko Ito, Tadao Okayasu, Shotaro Harada, Takefumi Kamakura, Kazuya Ono, Tatsuya Katsuno, Tatsuhide Tanaka, Kouko Tatsumi, Hiroshi Hibino, Hiroshi Hibino, Akio Wanaka, Tadashi Kitahara

“…Therefore, this study aimed to clarify these associations in mice.MethodsYoung C56BL/6 N (8 week old) and old (108–117 weeks old) mice were used in a micro-computed tomography (μCT) experiment for morphological analysis and a linear acceleration experiment for functional analysis. Young C56BL/6 N (8 week old) and middle-aged (50 week old) mice were used in electron microscopy experiments for morphological analysis.ResultsμCT revealed no significant differences in the otolith volume (p = 0.11) but significant differences in the otolith density (p = 0.001) between young and old mice. μCT and electron microscopy revealed significant differences in the structure of striola at the center of the otolith (μCT; p = 0.029, electron microscopy; p = 0.017). …”
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Identification and preliminary validation of biomarkers associated with mitochondrial and programmed cell death in pre-eclampsia by Rong Lin, Rong Lin, XiaoYing Weng, XiaoYing Weng, Liang Lin, Liang Lin, XuYang Hu, XuYang Hu, ZhiYan Liu, ZhiYan Liu, Jing Zheng, Jing Zheng, FenFang Shen, FenFang Shen, Rui Li, Rui Li

“…Clinical validation was conducted via real-time quantitative polymerase chain reaction (RT-qPCR), immunofluorescence, and Western blot.ResultsFour genes [solute carrier family 25 member 5 (SLC25A5), acyl-CoA synthetase family member 2 (ACSF2), mitochondrial fission factor (MFF), and phorbol-12-myristate-13-acetate–induced protein 1 (PMAIP1)] were identified as biomarkers distinguishing PE from normal controls. Functional analysis indicated their involvement in various biological pathways. …”
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The minor allele rs2295613(A), protective against systemic lupus erythematosus, makes a differential contribution to the activity of the <i>SLAMF1</i> gene promoter in T helpers an... by A. N. Uvarova, L. V. Putlyaeva, E. A. Zheremyan, E. M. Stasevich, M. M. Murashko, E. A. Bogomolova, K. V. Korneev, D. E. Demin, A. M. Schwartz, D. V. Kuprash

“…In the present work, we performed a functional analysis of single nucleotide promoter polymorphisms rs2295614(A&gt;T) and rs2295613(G&gt;A) of the SLAMF1 gene in the models of T-regulatory lymphocytes (MT-2 cell line) and T helpers (Jurkat cell line). …”
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Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network by Qi Xiao, Jingjing Shi, Lixian Wang, Guoping Zhao, Yanfei Zhang

“…Our results confirmed the critical roles of HOM3 and DIP5 in isopentanol production, along with genes associated with the aerobic respiratory chain (SDH3, CYT1, COX7, ROX1, and ATG41) and cofactor balance (BNA2 and NDE1). Additionally, functional analysis of the co-DEGs revealed that MAL33 is associated with the synthesis of branched-chain higher alcohols, expanding the intracellular metabolic network and offering new possibilities for green, cost-effective biofuel production.…”
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