OsBBX2 Delays Flowering by Repressing <i>Hd3a</i> Expression Under Long-Day Conditions in Rice by Yusi Yang, Jiaming Wei, Xiaojie Tian, Changhua Liu, Xiufeng Li, Qingyun Bu

“…The transcription of <i>OsBBX2</i> follows a diurnal rhythm under photoperiodic conditions, peaking at dawn. Functional analysis revealed that <i>OsBBX2</i> possesses transcriptional repression activity. …”
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Plant Probiotic Potential of Native Rhizobia to Enhance Growth and Sugar Content in <i>Agave tequilana</i> Weber var. Blue by Víctor Manuel Maranto-Gómez, Clara Ivette Rincón-Molina, Luis Alberto Manzano-Gómez, Adriana Gen-Jiménez, Julio César Maldonado-Gómez, Juan José Villalobos-Maldonado, Víctor Manuel Ruiz-Valdiviezo, Reiner Rincón-Rosales, Francisco Alexander Rincón-Molina

“…A comprehensive genomic and functional analysis was conducted for each strain to assess their plant probiotic traits. …”
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Changes in the soil and rhizosphere microbiomes associated with bacterial wilt decline in the tomato monoculture field by Junwei Peng, Jinfeng Hou, Hong Liu, Dmitri V. Mavrodi, Olga V. Mavrodi, Feifei Sun, Minchong Shen, Xia Wang, Keke Dang, Min Yan, Hui Liang, Yuanhua Dong, Jiangang Li

“…Greenhouse disease assays confirmed that the suppressive soil had significantly lower wilt incidence and shaped a healthy rhizosphere community characterized by reduced prevalence of pathogenic taxa and greater resource utilization diversity, compared to its disease-conducive counterpart. Moreover, functional analysis of the suppressive rhizosphere metagenome revealed enrichment of genes for the synthesis of antibiotics, polysaccharides, nitrogen metabolism, mineral absorption, and energy production. …”
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Identification and validation of expression and functions of ferroptosis-related gene HILPDA in early-onset preeclampsia placentas by Qianghua Wang, Xuegu Wang, Jiaojiao Fei, Chuanyue Jiang, Yafen Tao, Nana Yang, Huijuan Chen, Chengli Dou, Biao Ding, Danli Du, Xiang Li

“…Experimental validation included Western blot on clinical placental samples and siRNA-mediated knockdown in HTR-8/SVneo trophoblasts to assess migration.ResultsWe observed HILPDA upregulation and confirmed its diagnostic accuracy (AUC=0.71) in EOPE placentas. Functional analysis revealed HILPDA-associated enrichment in immune regulation (leukocyte migration, MHC complexes) and cellular processes (collagen organization, HIF-1 signaling). …”
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Genetic analysis of 25 Chinese pedigrees with neurofibromatosis type 1 and genotype-phenotype study from an extended cohort by Hongjun Fei, Xu Han, Yiyao Chen, Yan Xu, Chunxin Chang, Ming Li, Yanlin Wang, Jian Wang, Niu Li, Shuyuan Li

“…Conclusions We comprehensively described the clinical and genetic data of a Chinese NF1 cohort and emphasized the necessity of further functional analysis on splicing variants. Neurofibromas and bone lesions are age-dependent disease complications that exhibit progressive tendencies with increasing age in patients with NF1.…”
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Safety and feasibility evaluation of planning and execution of surgical revascularisation solely based on coronary CTA and FFRCT in patients with complex coronary artery disease: s... by Daniele Andreini, Kaoru Tanaka, Hideyuki Kawashima, Ronny R Buechel, William Wijns, Torsten Doenst, Jonathon Leipsic, Gregg W Stone, Giulio Pompilio, Antonio L Bartorelli, Saima Mushtaq, Enrico Ferrari, Francesco Maisano, Mark La Meir, Johan De Mey, Ulrich Schneider, Ulf Teichgräber, Faisal Sharif, Robbert de Winter, Brian Thomsen, Charles Taylor, Campbell Rogers, Yoshinobu Onuma, Patrick W Serruys

“…CCTA with FFRCT might provide better anatomical and functional analysis of the coronary circulation leading to appropriate anatomical and functional revascularisation, and thereby contributing to a better outcome.Ethics and dissemination Each patient has to provide written informed consent as approved by the ethical committee of the respective clinical site. …”
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Glycometabolism change during Burkholderia pseudomallei infection in RAW264.7 cells by proteomic analysis by Xuexia Li, Yingfei Zeng, Shengnan Guo, Chen Chen, Lin Liu, Qianfeng Xia

“…Furthermore, in vitro functional analysis revealed an increased glucose uptake and decreased ATP production and O-GlcNAcylation in the Bp group compared with control group, suggesting that B. pseudomallei HNBP001 infection induces changes in glycometabolism in RAW264.7 cells. …”
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Interleukin‐11 promotes lung adenocarcinoma tumourigenesis and immune evasion by Cristina Cirauqui, Laura Ojeda, Itziar Otano, Irene Pazos, Alba Santos, Eva M. Garrido‐Martín, Patricia Yagüe, Javier Ramos‐Paradas, Sonia Molina‐Pinelo, Giovanna Roncador, José Luis Solórzano, M. Teresa Muñoz, Patricia Cozar, Patricia Plaza, Rocío Suárez, Marta Jiménez, Roberto Moreno, Arantxa Rosado, Pablo Gámez, Ricardo García‐Luján, Jon Zugazagoitia, E. Alejandro Sweet‐Cordero, Mariano Barbacid, Amancio Carnero, Irene Ferrer, Luis Paz‐Ares

“…LUAD cell lines genetically engineered to overexpress or to silence IL‐11 or its receptor (IL‐11RA) were used for in vitro functional analysis and for in vivo experiments. Additionally, we used three different in vivo models: patient‐derived xenografts (PDXs), tobacco‐exposed mice and genetically engineered mouse models. …”
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Effector Pt31812 from Puccinia triticina acts as avirulence factor for Lr42-mediated resistance in wheat by Jianyuan Li, Jianyuan Li, Jiali Li, Jie Wei, Jie Wei, Lin Li, Yue Qi, Yue Qi, Yue Zhang, Yue Zhang, Wenxiang Yang, Qian-Hua Shen

“…Subcellular localization of Pt31812 was analyzed using transient expression on Nicotiana Benthamiana. Further functional analysis was conducted using transient expression and host-induced gene silencing (HIGS). …”
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Bioinformatics-driven exploration of key genes and mechanisms underlying oxidative stress in traumatic brain injury by Bin Ren, Jifang Liang, Leifang Yang, Xiaocong Wei, Min Guo, Hong Li

“…Machine learning analysis highlighted AKR1C2, QDPR, CYP3A5, CNTF, and PNPT1 as key genes with diagnostic potential (AUC &gt; 0.6). Functional analysis revealed significant involvement of these genes in immune processes and metabolic regulation. …”
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Integrated metabolomic and transcriptomic analysis reveals the role of root phenylpropanoid biosynthesis pathway in the salt tolerance of perennial ryegrass by Yan-Hua Cao, Zhao-Long Lü, Yuan-Hong Li, Yiwei Jiang, Jin-Lin Zhang

“…Antioxidant enzyme encoding genes (LpSOD1, LpCAT1), ion channel gene LpCaC1 and transcription factors (LpERFs, LpHSF1 and LpMYB1) were significantly upregulated in P2, suggesting their involvement in regulating expression of salt-responsive genes for salt tolerance. Functional analysis of DEGs revealed that biosynthesis of secondary metabolites, carbohydrate metabolism and signal transduction were the main pathways in response to salt stress. …”
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Identification of novel candidate loci for Alzheimer’s disease and related dementias by leveraging the shared genetic basis with hippocampal volume by Chenyang Jiang, Sven J. van der Lee, Niccolò Tesi, Wiesje M. van der Flier, Betty M. Tijms, Lianne M. Reus

“…By integrating summary statistics for these two traits, we identified both novel and previously reported ADRD-hippocampus loci. Functional analysis highlights the role of immune cells and lipid markers in the shared loci, suggesting a shared neurobiological basis for ADRD and bilateral hippocampal volumes.…”
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Probiotic-mediated modulation of gut microbiome in students exposed to academic stress: a randomized controlled trial by Jorge Francisco Vázquez-Castellanos, Lucas Ferreira Maciel, Lucas Wauters, Ann Gregory, Lukas Van Oudenhove, Karlien Geboers, Kristin Verbeke, Tamara Smokvina, Jan Tack, Tim Vanuytsel, Muriel Derrien, Jeroen Raes

“…The higher quantitative abundance of F. prausnitzii induced by the probiotic intake was associated with lowered self-reported anxiety levels before the exam. Functional analysis revealed minor changes upon intake of the probiotic strain. …”
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Exploring the functional variations of key candidate genes affecting egg production by hypothalamic-pituitary-ovarian axis in chickens by Dandan Wang, Yiqian Zhu, Jiajia Dong, Yihao Zhi, Guanghui Wei, Xiangtao Kang, Xiaojun Liu

“…Association analysis confirmed the significant association of chr3:79510218A>T with egg number from 21 to 43 week of age in Gushi chicken (P = 0.0022) and Guangxi Yao chicken (P = 0.0388), as well as with ovarian PHIP expression levels (P = 0.0010). Functional analysis indicated that the T allele of chr3:79510218A>T enhanced the transcriptional activity and upregulated PHIP expression in vitro by binding transcription factor forkhead box I1 (FOXI1). …”
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Integrated analysis of WGCNA and machine learning identified diagnostic biomarkers in trauma-induced coagulopathy by Qingsong Chen, Tao Li, Tao Zhang, Yue Zhou, Weifeng Huang, Hui Li, Li Shi, Jianxiao Li, Qi Zhang, Man Ma, Pan Wang, Hui Hu, Gongbin Wei, Jiangxia Xiang, Yuan Cheng, Jun Yang, Guangbin Huang, Yongming Li, Dingyuan Du

“…This approach included principal component analysis, differential gene expression analysis using DESeq2, Gene Set Enrichment Analysis (GSEA), weighted gene co-expression network analysis (WGCNA), and machine learning (ML) algorithms (support vector machine-recursive feature elimination, least absolute shrinkage and selection operator, and random forest) for feature gene identification. Functional analysis of genes and immunoinfiltration analysis were also conducted. …”
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Whole blood transcriptome profiles of trypanotolerant and trypanosusceptible cattle highlight a differential modulation of metabolism and immune response during infection by Trypan... by Peylhard, Moana, Berthier, David, Dayo, Guiguigbaza-Kossigan, Chantal, Isabelle, Sylla, Souleymane, Nidelet, Sabine, Dubois, Emeric, Martin, Guillaume, Sempéré, Guilhem, Flori, Laurence, Thévenon, Sophie

“…As expected, infection had a major impact on cattle blood transcriptome regardless of the breed. The functional analysis of differentially expressed genes over time in each breed confirmed an early activation of the innate immune response, followed by an activation of the humoral response and an inhibition of T cell functions at the chronic stage of infection. …”
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Genome and transcriptome analysis of Chinese wheat powdery mildew isolate 21–2 for discovery of important virulence determinants by Fansong Zeng, Bo Zhang, Bin Yuan, Minfeng Xue, Wenqi Shi, Shuangjun Gong, Tom Hsiang, Dazhao Yu, Lijun Yang

“…This study provides comprehensive genomic and transcriptomic insights into Chinese Bgt isolate 21–2, and reveals virulence determinants and their variants fundamental to pathogenesis. Future functional analysis of such genes will enhance our understanding of pathogenic mechanisms of powdery mildews.…”
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mTOR inhibition triggers mitochondrial fragmentation in cardiomyocytes through proteosome-dependent prohibitin degradation and OPA-1 cleavage by Hugo E. Verdejo, Valentina Parra, Andrea del Campo, Cesar Vasquez-Trincado, Damian Gatica, Camila Lopez-Crisosto, Jovan Kuzmicic, Leslye Venegas-Zamora, Ursula Zuñiga-Cuevas, Mayarling F. Troncoso, Rodrigo Troncoso, Beverly A. Rothermel, Mario Chiong, E. Dale Abel, Sergio Lavandero

“…Structural and functional analysis revealed increased mitochondrial fragmentation and impaired bioenergetics characterized by decreases in ROS production, oxygen consumption, and cellular ATP. …”
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Multi-omics analyses reveal altered gut microbial thiamine production in obesity by Yu Xia, Yu Xia, Longya Lu, Lulu Wang, Yanyan Qiu, Xingyin Liu, Weihong Ge, Weihong Ge, Weihong Ge

“…Integrated analysis indicated that thiamine deficiency was positively associated with the depletion of thiamine auxotrophic bacteria in the obese microbiome. Functional analysis revealed that KOs content for enzymes involved in the microbial production of thiamine were significantly lower in obesity, including tRNA uracil 4-sulfurtransferase (ThiI, PWilcoxon = 0.001) and nucleoside-triphosphatase (NTPCR, PWilcoxon = 0.006), both of which were positively associated with fecal thiamine.ConclusionOur study highlights the impairment of microbial thiamine production and its broad associations with gut microbiota dysbiosis and obesity-related phenotypes. …”
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Endophytic Diversity in Sicilian Olive Trees: Identifying Optimal Conditions for a Functional Microbial Collection by Dalila Crucitti, Stefano Barone, Salvadora Navarro-Torre, Paola Quatrini, Francesco Carimi, Tiziano Caruso, Davide Pacifico

“…Both farming systems favored an increase in the composition of microbial collection, though organic farming systems supported greater microbial richness. Functional analysis highlighted key PGP traits in a selection of bacterial isolates, including indole-3-acetic acid and siderophore production, nitrogen fixation, and antifungal activity. …”
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