A meta-analysis of whole blood transcriptome reveals genes associated with increased neutrophil activity and T cell suppression in sepsis by T. N. Shruthi, Anbazhagan Kolandaswamy, Prabakara Somanna

“…The genes identified through functional analysis could be used as potential diagnostic, prognostic, and therapeutic targets for sepsis conditions.…”
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Phosphorylation of Plant Ferredoxin-like Protein Is Required for Intensifying PAMP-Triggered Immunity in <i>Arabidopsis thaliana</i> by Tzu-Yi Chen, Rui-Wen Gong, Bo-Wei Chen, Yi-Hsien Lin

“…To investigate this, site-directed mutagenesis was used to generate PFLPT90A, a non-phosphorylatable mutant, and PFLPT90D, a phospho-mimetic mutant, for functional analysis. Based on the experimental results, none of the recombinant proteins were able to enhance the hypersensitive response induced by the HrpN protein or increase resistance to the soft rot pathogen <i>Pectobacterium carotovorum</i> subsp. …”
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Functional circuits of LYL1 controlled by supraphysiological androgen in prostate cancer cells to regulate cell senescence by Mehdi Heidari Horestani, Katrin Schindler, Aria Baniahmad

“…Regulatory feedback loops were identified by rescue experiments. Functional analysis revealed that KD of BHLHE40 reduces whereas LYL1 KD enhances p27kip1 levels. …”
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Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life. by Guanxiang Liang, Nilusha Malmuthuge, Thomas B McFadden, Hua Bao, Philip J Griebel, Paul Stothard, Le Luo Guan

“…The expression levels of miR-15/16, miR-29 and miR-196 were positively correlated with the copy numbers of 16S rRNA gene of Bifidobacterium or Lactobacillus species or both (P<0.05). Functional analysis using Ingenuity Pathway Analysis identified the above mentioned differentially expressed miRNAs as potential regulators of gut tissue cell proliferation and differentiation. …”
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Evaluation of Wuchereria bancrofti GST as a vaccine candidate for lymphatic filariasis. by Anandharaman Veerapathran, Gajalakshmi Dakshinamoorthy, Munirathinam Gnanasekar, Maryada Venkata Rami Reddy, Ramaswamy Kalyanasundaram

“…Isotype analysis of the anti-WbGST IgG antibodies showed a predominance of IgG1 and IgG3 antibodies in EN individuals. Subsequent functional analysis of the rWbGST showed that the rWbGST protein retained the enzymatic activity of GST and the antibodies in EN sera could inhibit this enzymatic activity. …”
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Screening of CAD-related secretory genes associated with type II diabetes based on comprehensive bioinformatics analysis and machine learning by Li Xie, Han Xiao, Maoyu Zhao, Li Xu, Si Tang, Youzhu Qiu

“…Additional analyses included immune analysis, drug sensitivity, competing endogenous RNA (ceRNA) networks, and smooth muscle cell -related functional analysis. Results An abnormally high proportion of smooth muscle cells was observed in CAD tissues compared to normal cardiomyocytes. …”
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Structurally engineered monolithic catalyst for enhanced sunlight-driven degradation of ciprofloxacin in contaminated water systems by Iqra Mazhar, Muhammad Afzaal, Muhammad Altaf, Manzar Sohail, Raja Shahid Ashraf, Abdul-Sattar Nizami

“…Morphological and functional analysis exhibited evidence of metallic silver formation. …”
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Bioinformatics and Functional Validation of <i>CqPRX9L1</i> in <i>Chenopodium quinoa</i> by Hongxia Guo, Linzhuan Song, Yufa Wang, Li Zhao, Chuangyun Wang

“…In this study, a preliminary functional analysis of <i>CqPRX9L1</i> was conducted. Bioinformatics analysis revealed that <i>CqPRX9L1</i> encodes a 349-amino acid protein belonging to the plant-peroxidase-like superfamily, featuring a transmembrane domain and cytoplasmic localization. …”
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Bioinformatics analysis identifies WNK1 gene as a potential biomarker for cholangiocarcinoma diagnosis and immune infiltration by Qi Sun, Xianli Lei, Xiangrong Meng, Caijun Zha, Lei Yan, Wenjing Zhang

“…Results: Differential expression of WNK1 was observed between CHOL and normal samples, suggesting its diagnostic value. Functional analysis showed that WNK1 and its associated genes were primarily enriched in pathways such as leukocyte transendothelial migration and chemokine signaling. …”
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Genome wide identification and expression profiling of Bcl2 associated anthanogene family cochaperones reveals abiotic stress response in Ricinus communis L. by Muhammad Arif, Shuzhen Men, Ayesha Fazal Nawaz, Hina Abbas, Wenqi Shi, Mohamed A. El-Sheikh, Parvaiz Ahmad, Ruhong Xu, Luhua Li

“…Quantitative real-time polymerase chain reaction (qRT-PCR) investigation revealed that 9 distinct RcBAG genes were strongly induced after cold and heat treatments. Functional analysis and protein–protein interactions were used to predict the potential regulatory network of RcBAGs, revealing tight networking and signaling with HSP proteins. …”
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Metabolic profiling of glioblastoma and identification of G0S2 as a metabolic target by Jianlei Kang, Yujie Xu, Qitai Zhao, Ying Wang, Zhenyan He, Xin Xu

“…The predictive model showed robust performance in forecasting the survival of glioma patients. Functional analysis identified G0S2 as a key metabolic regulator highly expressed in gliomas. …”
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Genome‐wide screening of abberant methylated drivers combined with relative risk loci in bladder cancer by Chuanjie Zhang, Kangjie Shen, Yuxiao Zheng, Feng Qi, Jun Luo

“…MethylMix package was used for identifying methylation‐driven genes and functional analysis was mainly performed based on ConsensusPathDB database. …”
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Genomic Signatures of Positive Selection and Local Adaptation in Ethiopian Sheep Populations by B. E. Demissie, G. M. Tarekegn, H. Dadi, Z. Edea, A. A. Woldesemayat, K-S Kim, S. Girma, T. Seyoum, K. Melaku, S. Keskes

“…Fixation index (FST) and cross-population extended haplotype homozygosity (XPEHH) methods were used to detect signatures of positive selection. Functional analysis revealed genes related to plateau adaptation, immune response, and tail fat formation. …”
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Prominence of Microbiota to Predict Fibrous Stenosis in Crohn&rsquo;s Disease by Yang X, Pan Y, Gao CP, Li H, Zhang YH, Huang CL, Cao L, Xiao SY, Zhou Z

“…Furthermore, LEfSe analysis indicated that g_Bacteroides and g_Enterocloster could predict intestinal fibrous stenosis while p_Actinobacteria, c_Actinomycetia, c_Bacilli, o_Lactobacillales, f_Streptococcaceae and g_Streptococcus could predict CD without stenosis. Functional analysis revealed differential enrichment in five metabolic pathways at the KEGG pathway level in CD patients with fibrous stenosis, including sphingolipid metabolism, lipoic acid metabolism, and biosynthesis of neomycin, kanamycin and gentamicin. …”
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Comprehensive analysis identifies endocrine fibroblast growth factors as promising prognostic markers for colorectal carcinoma by Leili Rejali, Moein Piroozkhah, Mana Jahanbin, Pooya Jalali, Binazir Khanabadi, Elahe Daskar Abkenar, Zeinab Asghari, Mehrdad Hashemi, Amir Sadeghi, Zahra Salehi, Ehsan Nazemalhosseini-Mojarad

“…Through integrated analyses of diverse databases, including TIMER2.0, UALCAN, OncoDB, cBioPortal, LinkedOmics, STRING, htfTarget, mirTarBase, circBank, and DGIdb, we explored eFGFs’ gene expression, DNA methylation, prognostic significance, genetic alterations, gene regulatory networks, functional analysis, and drug interactions in COAD patients. …”
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The ZmPIF4.1-ZmPTI1c-ZmMYB31 module regulates maize immunity to Gibberella stalk rot caused by Fusarium graminearum by Fugui Xie, Liang Ma, Huilan Zhang, Onyino Johnmark Okello, Junjie Cui, Qing Wang, Xiquan Gao

“…We found in this study that GSR resistance is regulated by the ZmPIF4.1 (Phytochrome-Interacting Factor 4)-ZmPTI1c (Pto-Interacting 1)-ZmMYB31 module coupled with photoperiod. The functional analysis of zmpti1c mutant indicated that ZmPTI1c negatively regulates maize GSR resistance. …”
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Characterization of the Avian Mitochondrial-Derived Peptide MOTS-c and Its Potential Role as a Metabolic Regulator by Xin Shu, Jiying Liu, Bingjie Xu, Hui Wang, Li Liu, Xiaotong Zheng, Jianfei Chen

“…Fasting significantly reduced heart <i>MOTS-c</i> expression, suggesting potential metabolic regulatory functions. Functional analysis of MOTS-c in primary hepatocytes revealed significant enrichment of the ribosome, oxidative phosphorylation, and key signaling pathways (PI3K-AKT and JAK-STAT) following 24 hours of treatment. …”
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Palmitoylation regulators drive the progression of clear cell renal cell carcinoma through Inhibition of cuproptosis: insights into the role of ZDHHC18 by Wei Xu, Xiao-chao Chen, Yang Wang, Jian-chun Chen, Zhi-jun Cao, Ru Huang, Chao Chen, Dao-rong Hou, Min-jun Jiang, Chen Xu

“…We developed a risk model and a nomogram based on palmitoylation scores to enhance risk classification. Functional analysis indicated that high-risk patients exhibited disrupted fatty acid metabolism. …”
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Galectin-8 drives ERK-dependent mitochondrial fragmentation, perinuclear relocation and mitophagy, with metabolic adaptations for cell proliferation by Adely de la Peña, Claudio Retamal, Francisca Pérez-Molina, Nicole Díaz-Valdivia, Francisco Veloso-Bahamondes, Diego Tapia, Jorge Cancino, Felix Randow, Alfonso González, Claudia Oyanadel, Andrea Soza

“…Bafilomycin A abrogates Gal-8-induced cell proliferation, suggesting that mitophagy serves as an adaptation to cell proliferation demands. Functional analysis under Gal-8 stimulation shows that mitochondria maintain an active electron transport chain, partially uncoupled from ATP synthesis, and an increased membrane potential, indicative of healthy mitochondria. …”
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Kindlin-3 phosphorylation is crucial for thrombosis and hemostasis in vivo by Elzbieta Pluskota, Dorota Szpak, Yunmei Wang, Jun Qin, Daniel I. Simon, Edward F. Plow, Katarzyna Bialkowska

“…Results: Platelets isolated from S485A kindlin-3 mice did not undergo agonist-induced kindlin-3 phosphorylation. Functional analysis revealed that S485A kindlin-3 mice exhibited prolonged tail bleeding time, increased blood loss, and delayed thrombosis in the carotid artery injury model in vivo. …”
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