Genotype‐function‐phenotype correlations for SCN1A variants identified by clinical genetic testing by Andrew T. Knox, Christopher H. Thompson, Dillon Scott, Tatiana V. Abramova, Bethany Stieve, Abigail Freeman, Alfred L. George Jr.

“…Interpretation In SCN1A‐associated epilepsy, functional analysis and neuron simulation studies resolved variants of uncertain significance and correlated with aspects of phenotype and medication response.…”
Get full text

Characteristics of gut microbiota in people with circadian rhythm disruption and its correlation with cognition by JIAN Jincheng, HE Wei, JIANG Hongfei

“…Additionally, KEGG functional analysis showed that the experimental group had obviously higher expression levels in Th17 cell differentiation and the IL-17 signaling pathway than the control group (P<0.05). …”
Get full text

DNA extraction protocols for animal fecal material on blood spot cards. by Ann-Katrin Llarena, Thomas H A Haverkamp, Wenche Støldal Gulliksen, Kristin Herstad, Arne Holst-Jensen, Eystein Skjerve, Lisbeth Rannem, Sabrina Rodriguez-Campos, Øivind Øines

“…We found that higher number of reads increased the richness of observed genera between 100,000 and 5 million reads, after which higher sequencing effort did not increase the richness of the metagenomes. As for functional analysis, the number of low abundance functions in the metagenomes of the animals' feces increased with sequencing depth above 20 million PE reads.…”
Get full text

Regulation and Functions of Long Noncoding RNAs During Meloidogyne incognita Parasitism of Tomato by Selin Ozdemir, Sobhan Bahrami Zadegan, Mst Shamira Sultana, Nicole Coffey, J. Hollis Rice, Tarek Hewezi

“…Overexpressing five of these lncRNAs significantly increased tomato resistance to M. incognita, supporting the functional importance of lncRNAs for establishing tomato-M. incognita interaction. Functional analysis of the target mimicry of lncRNAs towards miRNAs resulted in the identification of two novel regulatory modules involving miR47 and miR156e-5p and their targeted genes that regulate tomato responses to M. incognita parasitism. …”
Get full text

Light-dark dependent changes in chloroplast and mitochondrial activity in Chlamydomonas reinhardtii by Gunjan Dhawan, Gunjan Dhawan, Basuthkar Jagadeeshwar Rao, Basuthkar Jagadeeshwar Rao

“…Spatial mapping showed tubular mitochondria positioned peripherally to the chloroplast cup in light, whereas fragmented and intermediate mitochondria were diffused around distorted chloroplast in dark, which again gets reversed in light. Functional analysis using 77K spectroscopy and photosynthetic protein levels (PsaA and D1) reflected that PSI/PSII fluorescence ratio remains stable in continuous light condition but increased exceptionally in continuous dark, which led to transient changes observed in fluorescence ratio in light/dark-dependent manner in synchronous cultures. …”
Get full text

INDUCED PLURIPOTENT STEM CELL-DERIVED MICROGLIA TO STUDY NLRP3 INFLAMMASOME ACTIVATION IN ALZHEIMER’S DISEASE by Aslı Erdoğan, Cira Dansokho, Jari Koıstınaho, Sarka Lehtonen, Seyhun Solakoğlu, Michael Thomas Heneka

“…The expression of proteins associated with the NLRP3 pathway was investigated by Western blot. For functional analysis, cytokine release was assessed by Enzyme-linked immunosorbent assay (ELISA) upon NLRP3 inflammasome activators (lipopolysaccharide (LPS), Aβ) and inhibitor (cytokine release inhibitory drug 3, CRID3) treatments. …”
Get full text

Systematic exploration of the molecular characteristics of CD8+ T cells to predict the response to immunotherapy and the prognosis of patients with colon adenocarcinoma by Xin Shen, Lifeng Shang, Junwei Han, Yi Zhang, Wenkai Niu, Haiwang Liu, Hai Shi

“…Through in vitro experiments, we suggested that TSPYL2 may act as an oncogene in COAD. Through functional analysis, a high expression of genes linked to the cell cycle, cytoskeleton, cell adhesion, and various cancer-related pathways was observed in the high CD8S group, which aids in tumor invasion. …”
Get full text

Potential immunomodulatory effects of the extract from Artemisia frigida Willd on loaches infested with Aeromonas hydrophila revealed by microRNA analysis by Yue Zhao, Yue Zhao, Yue Zhao, Yuqing Qiu, Lishang Dai, Hong Wang

“…Further target gene prediction and functional analysis of 30 differential miRNAs showed that the target genes of these miRNAs were involved in the regulation of several innate and antibacterial immunity-related pathways, including endocytosis, apoptosis, phosphatidylinositol signaling system, RLR signaling pathway, TLR signaling pathway and NLR signaling pathway. …”
Get full text

Severity-specific immune landscape of COVID-19 revealed by single-cell sequencing by Hongying Zhao, Meiting Fei, Wentong Yu, Zhichao Geng, Jing Bai, Li Wang

“…For example, RPS26 was down-regulated, while the ZFP36, IL-32, and IgM genes were up-regulated with increasing disease severity. Functional analysis showed multiple immune-related pathways, such as interleukin-2 and interleukin-10 production pathways, were dysregulated. …”
Get full text

Genome-wide identification and classification of MYB superfamily genes in peach. by Chunhua Zhang, Ruijuan Ma, Jianlan Xu, Juan Yan, Lei Guo, Juan Song, Ruchao Feng, Mingliang Yu

“…This study laid the foundation for further functional analysis of MYB superfamily genes in peach and enriched the knowledge of MYB superfamily genes in plant species.…”
Get full text

Microecological mechanisms of mountainous forest cultivated ginseng growth vigor and saponin accumulation, and the characterization of bionic microbial fertilizer by Fengyu Pang, Yugang Gao, Zhenqi Zhuang, Changju Li, Yan Zhao, Qun Liu

“…IntroductionA study on the soil microecological mechanisms influencing the growth vigor and saponin accumulation of mountainous forest cultivated ginseng (MFCG) under various forest types.MethodsUsing MFCG from different forest types as experimental material, the correlation and functional analysis of MFCG growth vigor, ginseng saponin content, and soil nutrient elements in their rhizosphere were conducted to clarify the soil microecological mechanisms by which different forest types affect the growth vigor and saponin accumulation of understory ginseng. …”
Get full text

Enterotype-stratified gut microbial signatures in MASLD and cirrhosis based on integrated microbiome data by Heng Yuan, Heng Yuan, Junyu Zhou, Junyu Zhou, Xuangao Wu, Shiwei Wang, Sunmin Park, Sunmin Park

“…In ET-P, Prevotella hominis and Clostridium saudiense were significantly associated with cirrhosis. Functional analysis revealed reduced biosynthesis of fatty acids, proteins, and short-chain fatty acids (SCFAs), coupled with increased lipopolysaccharide(LPS) production and altered secondary bile acid metabolism in MASLD and cirrhosis patients. …”
Get full text

HARDWARE SUPPORT PROCEDURES FOR ASYMMETRIC AUTHENTICATION OF THE INTERNET OF THINGS by Петро Сергійович Клімушин, Тетяна Миколаївна Соляник, Олександр Олександрович Можаєв, Віталій Вікторович Носов, Тетяна Петрівна Колісник, Василь Вікторович Янов

“…Research methods: method of structural and functional analysis and design of complex systems, methods of identification and authentication of information objects, cryptographic methods of information protection, methods of security analysis of distributed information systems. …”
Get full text

Tensorial Maclaurin Approximation Bounds and Structural Properties for Mixed-Norm Orlicz–Zygmund Spaces by Waqar Afzal, Mujahid Abbas, Mutum Zico Meetei, Saïd Bourazza

“…Given the limited research on such spaces, our findings contribute valuable insights to the functional analysis.…”
Get full text

CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Notably, activation of ATF3 transcription was demonstrated to upregulate the expression of its downstream target ACSL4. Functional analysis indicated that circBAZ1B promoted ATF3 expression via miR-1252-5p. …”
Get full text

Chromosome X Open Reading Frame 38 (CXorf38) Is a Tumor Suppressor and Potential Prognostic Biomarker in Lung Adenocarcinoma: The First Characterization by Rui Yan, Heng-Wee Tan, Na-Li Cai, Le Yu, Yan Gao, Yan-Ming Xu, Andy T. Y. Lau

“…Clinical data showed <i>CXorf38</i> downregulation with lung cancer tissues of smokers, indicating a potential role in smoking-induced cancer progression and treatment. Functional analysis using bioinformatics linked CXorf38 to immune response regulation, suggesting involvement in the tumor immune microenvironment. …”
Get full text

Gut microbiome in patients with early-stage and late-stage endometriosis by Zhaoxia Cai, Ziwei Zhou, Sixia Huang, Song Ma, Yuying Chen, Yuzhen Cao, Ying Ma

“…Conclusion The gut microbial community structure in early-stage endometriosis patients significantly differs from that in late-stage cases, with late-stage patients experiencing dysmenorrhea displaying particularly distinct gut profiles. Predicted functional analysis indicated suppressed steroid biosynthesis pathways in the gut of late-stage endometriosis patients. …”
Get full text

Genome‐Wide Association Analyses Identify Hydrogen Peroxide–Responsive Loci in Wheat Diversity by Mohammad Kamruzzaman, Md. Nurealam Siddiqui, Samira Rustamova, Agim Ballvora, Jens Léon, Ali Ahmad Naz

“…These findings provide a fundamental basis for functional analysis of putative candidate genes linked to H2O2‐mediated root–shoot growth of wheat. …”
Get full text

Microbial-derived peptidases are altered in celiac disease, non-celiac gluten sensitivity, and functional dyspepsia: a systematic review and re-analysis of the duodenal microbiome by Jennifer C. Pryor, Cheenie Nieva, Nicholas J. Talley, Guy D. Eslick, Kerith Duncanson, Grace L. Burns, Emily C. Hoedt, Simon Keely

“…Re-analysis of duodenal 16S rRNA data was possible for five included articles but did not identify any consistent differentially abundant taxa. Predicted functional analysis of the microbiome revealed that peptidases including aminopeptidase, proline iminopeptidase, and Xaa-Pro dipeptidase are altered in CeD, NCGS, and FD, respectively. …”
Get full text

Evaluating the Impact of Environmental Factors on Bacterial Populations in Riverine, Estuarine, and Coastal Sediments by Ramganesh Selvarajan, Ming Yang, Henry J. O. Ogola, Timothy Sibanda, Akebe Luther King Abia

“…LEfSe (Linear discriminant analysis Effect Size) analysis identified specific genera within the estuary, including <i>Bacillus</i> (20.26%), unclassified_<i>Paenibacillus</i> (12.87%), <i>Clostridium</i> (3.81%), <i>Gailella</i> (3.17%), <i>Paenibacillus</i> (3.02%), <i>Massilia</i> (1.70%), <i>Paraburkholderia</i> (1.42%), and <i>Pantoea</i> (1.15%), as potential biomarkers for habitat health. Functional analysis revealed an elevated expression of the genes associated with ABC transporters and carbon metabolism in the estuary, suggesting a heightened nutrient cycling capacity. …”
Get full text