Epigenome-wide DNA methylation analysis reveals differentially methylation patterns in skeletal muscle between Chinese Chenghua and Qingyu pigs by Kai WANG, Ping-xian WU, Shu-jie WANG, Xiang JI, Dong CHEN, An-an JIANG, Wei-hang XIAO, Yan-zhi JIANG, Li ZHU, Yang-shuang ZENG, Xu XU, Xiao-tian QIU, Ming-zhou LI, Xue-wei LI, Guo-qing TANG

“…We detected 10 699 differentially methylated cytosines (DMCs) and 2 760 differentially methylated genes (DMGs) associated with these DMCs. Functional analysis showed that these DMGs were mainly enriched in the AMPK signaling pathway, Type II diabetes mellitus, Insulin signaling pathway, mTOR signaling pathway, and Insulin resistance. …”
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Identification of clinical diagnostic and immune cell infiltration characteristics of acute myocardial infarction with machine learning approach by Huali Jiang, Weijie Chen, Benfa Chen, Tao Feng, Heng Li, Dan Li, Shanhua Wang, Weijie Li

“…A total of 134 upregulated and 25 downregulated genes were identified. Functional analysis showed that the dysregulated genes were involved in cytokine- and immune-related signaling. …”
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Does preeclampsia impact the gut microbiota of preterm offspring during early infancy? by Yang Chen, Zhou-Ting Yi, Hai-Long Yu, Xiao-Yan Wu, Jun-Ping Wang, Chuan Nie, Hui Li, Sheng-Hui Li, Qiu-Long Yan, Tian-Wen He, Min-Chai Chen, Xin-Yue Yang, Ji-Ying Wen, Li-Juan Lv

“…The gut virome analysis showed no significant differences among the groups. Functional analysis revealed distinct metabolic pathway activities across the groups, suggesting that early disturbances due to PE impact the establishment of healthy gut microbiota. …”
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Genome-Wide Identification and Expression Profiling of Glycosidases, Lipases, and Proteases from Invasive Asian Palm Weevil, <i>Rhynchophorus ferrugineus</i> by Nazmi Harith-Fadzilah, Mohammad Nihad, Mohammed Ali AlSaleh, Abdulqader Yaslam Bazeyad, Subash-Babu Pandurangan, Kashif Munawar, Arya Vidyawan, Hattan A. Alharbi, Jernej Jakše, Arnab Pain, Binu Antony

“…Overall, our study serves as a foundation for further functional analysis and offers a novel target for the development of a novel bio-rational insecticide for <i>R. ferrugineus</i>.…”
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Atypical Presentation of IARS1‐Related Disorder: Expanding the Phenotype and Genotype by Parith Wongkittichote, Kira E. Jonatzke, Benjamin T. Hyde, Lance W. Peterson, Mai He, Robert C. McKinstry, Anthony Antonellis, Marwan Shinawi

“…The frameshift variant is predicted to cause a loss of function, and functional analysis of the p.Asp249Gly variant was performed using baker's yeast. …”
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CRISPR-Cas9-mediated deletions of FvMYB46 in Fragaria vesca reveal its role in regulation of fruit set and phenylpropanoid biosynthesis by Arti Rai, Magne Nordang Skårn, Abdelhameed Elameen, Torstein Tengs, Mathias Rudolf Amundsen, Oskar S. Bjorå, Lisa K. Haugland, Igor A. Yakovlev, May Bente Brurberg, Tage Thorstensen

“…The MYB46 transcription factor is a key regulator of secondary cell wall structure, lignin and flavonoid biosynthesis in many plants, but little is known about its activity in flowers and berries in F. vesca. For functional analysis of FvMYB46, we designed a CRISPR-Cas9 construct with an endogenous F. vesca-specific U6 promoter for efficient and specific expression of two gRNAs targeting the first exon of FvMYB46. …”
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Genome-Wide Analysis of Mlo Genes and Functional Characterization of <i>Cm</i>-<i>mlo38</i> and <i>Cm</i>-<i>mlo44</i> in Regulating Powdery Mildew Resistance in Melon by Fangyi Gong, Yanhong Lan, Tian Zhang, Chun Li, Yifan Li, Feng Xia, Xiaojun Liu, Duchen Liu, Genyun Liang, Peng Cai, Chao Fang

“…These results provide a comprehensive understanding of the Mlo family in <i>Cucurbitaceae</i> and pave the way for future functional analysis and genetic improvement for improving PM resistance in <i>Cucurbitaceae</i>.…”
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Efficacy of asfotase alfa in a patient with adult-onset hypophosphatasia without obvious bone lesions: a case report with review of literature by Seiji Nishikage, Masaaki Yamamoto, Takahiro Niikura, Yuiko Inaba, Tomoyuki Akiyama, Risa Harada, Yoshitada Sakai, Kenji Sugawara, Kanako Tachikawa, Toshimi Michigami, Wataru Ogawa, Hidenori Fukuoka

“…Despite the absence of bone lesions, the patient was diagnosed with HPP based on persistently low alkaline phosphatase levels, family history, and a novel heterozygous ALPL variant (p.Ala205Thr). Functional analysis revealed a dominant-negative effect for this variant. …”
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Genome-wide association analysis identified the involvement of MRPS22 in the regulation of Muscovy duck broodiness by Hailiang Yu, Shiqi Yu, Wanli Yang, Weihuang Lin, Xiaowei Yang, Xiao Wang, Cheng Zhang, Liping Guo, Xingyong Chen

“…The key genes annotated to these SNPs loci include NCOA6, MRPS22, SCAP, CRY2, CK1δ and EZH1, which could be candidate genes regulating the broodiness of Muscovy ducks. Functional analysis showed that over expression of MRPS22 upregulated the expression of CYP19A1 to promote the synthesis of intracellular estradiol, and downregulated the expression of CYP11A1 and 3β-HSD to inhibit the synthesis of progesterone to regulate broodiness of Muscovy ducks. …”
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Magnetic resonance evaluation of rotator cuff healing after surgical repair of large and massive lesions using the load-sharing rip-stop construct: encouraging results by Raul Meyer Kautsky, MD, Benno Ejnisman, MD, PhD, Juarez Leite Junior, MD, Eduardo Antônio de Figueiredo, MD, PhD, Ricardo Azeredo Costa, MD, Jair Simmer Filho, MSc, MD

“…Results: Eighteen shoulders were included in the study and all of them performed a postoperative MRI; 1 patient experienced tendon rerupture on follow-up MRI, corresponding to a 5.56% failure rate. In the functional analysis, 16 shoulders (88.89%) scored above 80 on the American Shoulder and Elbow Surgeons score and had satisfactory results on the University of California, Los Angeles score (rated as excellent and good). …”
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Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa by Seung-Hun Kim, Kwang-Hwan Choi, Dong-Kyung Lee, Mingyun Lee, Jae Yeon Hwang, Chang-Kyu Lee

“…However, the OCT4 promoter and four regulatory regions, including distal and proximal enhancer elements, had high similarity. Next, a functional analysis of the porcine OCT4 promoter region was conducted. …”
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Identification, Characterization, and Ultrastructure Analysis of the Phenol-Degrading <i>Rhodococcus erythropolis</i> 7Ba and Its Viable but Nonculturable Forms by Valentina N. Polivtseva, Anton N. Zvonarev, Olesya I. Sazonova, Yanina A. Delegan, Yulia N. Kocharovskaya, Alexander G. Bogun, Nataliya E. Suzina

“…The following distinctive features were found: modifications (thickening) of cell membranes, cell size reduction, nucleoid condensation. Functional analysis of the genome showed the presence of genes for the degradation of alkanes, and two branches of the β-ketoadipate pathway for the degradation of aromatic compounds. …”
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Construction of a novel inflammatory-related prognostic signature of acute myelocytic leukemia based on conjoint analysis of single-cell and bulk RNA sequencing by Yongfen Huang, Ping Yi, Ping Yi, Yixuan Wang, Lingling Wang, Yongqin Cao, Jingbo Lu, Kun Fang, Kun Fang, Yuexin Cheng, Yuqing Miao, Yuqing Miao

“…This study aims to construct a novel risk model for AML patient through comprehensive analysis of scRNA and bulk RNA data to optimize the precise treatment strategies for patients and improve prognosis.Methods and ResultsscRNA-seq classified cells into nine clusters, including Bcells, erythrocyte, granulocyte-macrophage progenitor (GMP), hematopoietic stem cell progenitors (HSC/Prog), monocyte/macrophagocyte (Mono/Macro), myelocyte, neutrophils, plasma, and T/NK cells. Functional analysis demonstrated the important role of inflammation immune response in the pathogenesis of AML, and the leukocyte transendothelial migration and adhesion in the process of inflammation should be noticed. ssGSEA method identified four core cells including GMP, HSC/Prog, Mono/Macro, and myelocyte for subsequent analysis, which contains 1,594 marker genes. …”
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The Gene Expression Profile of Milk Somatic Cells of Small Ruminant Lentivirus-Seropositive and -Seronegative Dairy Goats (<i>Capra hircus</i>) During Their First Lactation by Joanna Pławińska-Czarnak, Alicja Majewska, Joanna Magdalena Zarzyńska, Jarosław Kaba, Emilia Bagnicka

“…RT-qPCR confirmed changes for <i>DUSP26</i>, <i>SCARA3</i>, and <i>APBB2</i>. Functional analysis indicated associations with immune processes and HIV-like pathways. …”
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Two Nonsense GLI3 Variants Are Identified in Two Chinese Families With Polydactyly by Yongzhen Qi, Kai Liu, Yuda Wei, Xiaxia Liu, Liangqian Jiang, Juan Teng, Baoqiang Chong, Shuqi Zheng, Xiangyu Zhao, Lin Li

“…No significant difference in transcription levels between the mutations and wild type was observed. Functional analysis revealed that the truncated variants p. …”
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Functional mass spectrometry indicates anti-protease and complement activity increase with COVID-19 severity by Douglas D. Fraser, Douglas D. Fraser, Swapan Roy, Matt Kuruc, Maritza Quintero, Logan R. Van Nynatten, Gediminas Cepinskas, Gediminas Cepinskas, Haiyan Zheng, Amenah Soherwardy, Devjit Roy

“…As protein function is often not directly proportional to protein abundance, and mechanistic pathways are interconnected and under constant feedback regulatory control, functional analysis is required. In this study, we used functional mass spectrometry to measure anti-protease and complement activity in plasma obtained from coronavirus disease 2019 (COVID-19) patients. …”
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Biotype and host relatedness influence the composition of bacterial microbiomes in Schizaphis graminum aphids by Yan M. Crane, Yan M. Crane, Charles F. Crane, Charles F. Crane, Christian Webb, Christian Webb, Brandon J. Schemerhorn, Brandon J. Schemerhorn

“…By counts of differentially populated genera, the factors biotype, host plant species, infestation time, and host plant resistance genes to greenbug, were consecutively less important. Functional analysis with PICRUSt2 illustrated a diminution of respiratory electron transport and long-chain fatty acids in the Buchnera endosymbiont, reflecting adaptation to an intracellular environment.…”
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Genome-wide analyses reveal intricate genetic mechanisms underlying egg production efficiency in chickens by Lizhi Tan, Xinyu Cai, Yuan Kong, Zexuan Liu, Zilong Wen, Lina Bu, Yuzhan Wang, Xiaojun Liu, Zhiwu Zhang, Jianlin Han, Dandan Wang, Yiqiang Zhao

“…By combining the signals of Singleton Density Score (SDS), which is a population-genetic statistic designed to detect recent selection by leveraging the distribution of singletons, and association analyses, multiple egg-laying traits related to egg production efficiency were found to have experienced polygenic selection. Consistently, functional analysis of associated genes demonstrates that egg production efficiency benefits from multiple physiological functions. …”
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Characterization of microbial communities in flavors and fragrances during storage by Yingjie Feng, Tingting Zhang, Jinchu Yang, Wenzhao Liu, Yongfeng Yang, Jihong Huang, Shen Huang, Zongcan Yang, Qianjin Liu, Wenchao Zheng, Qing Zhou, Qing Zhou

“…In addition, the bacterial species Oceanobacillus sojae and Niallia nealsonii showed significant increases in relative abundance, suggesting that bacteria were one of the key contributors to the spoilage of flavors and fragrances. Functional analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database indicated a shift in metabolic pathways within the microbial community, with heightened metabolic activity correlating with spoilage. …”
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Preclinical Efficacy and Proteomic Prediction of Molecular Targets for s-cal14.1b and s-cal14.2b Conotoxins with Antitumor Capacity in Xenografts of Malignant Pleural Mesothelioma by Angélica Luna-Nophal, Fernando Díaz-Castillo, Vanessa Izquierdo-Sánchez, Jesús B. Velázquez-Fernández, Mario Orozco-Morales, Luis Lara-Mejía, Johana Bernáldez-Sarabia, Noemí Sánchez-Campos, Oscar Arrieta, José Díaz-Chávez, Jorge-Ismael Castañeda-Sánchez, Alexei-Fedorovish Licea-Navarro, Saé Muñiz-Hernández

“…The in silico protein functional analysis showed modifications in the biological pathways associated with carcinogenesis. …”
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