Association of the PINX1 Variant rs6984094, Which Lengthens Telomeres, with Systemic Lupus Erythematosus Susceptibility in Chinese Populations by Yuan-yuan Qi, Xin-ran Liu, Ying-xin He, Min Zhou, Xiang-hui Ning, Ya-ling Zhai, Xiao-xue Zhang, Xiao-yang Wang, Ya-fei Zhao, Yan Cui, Zhan-Zheng Zhao

“…Multiple layers of functional analysis suggested that the PINX1 rs6984094 risk T allele exhibited increased nuclear protein binding. …”
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A Novel Variant in Dentin Sialophosphoprotein (DSPP) Gene Causes Dentinogenesis Imperfecta Type III: Case Report by Yan Wang, Ximin Xu, Yuzhe Ding, Guohua Yuan

“…Conclusion Taken together, this study describes the clinical features and genetic etiology of a family with DGI‐III, expanding the range of variants that cause DGI‐III and enriching the phenotypes associated with variants in the signal peptide segment of DSPP. Functional analysis reveals that this variant disrupts DSPP protein secretion.…”
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SWEET transporters and their potential roles in response to abiotic and biotic stresses in mulberry by Xiaoru Kang, Shuai Huang, Yuming Feng, Rumeng Fu, Fengjuan Tang, Longyan Zheng, Peijun Li, Nan Chao, Li Liu

“…However, SWEETs in mulberry are still poorly studied without a comprehensive functional analysis of SWEETs. In the present study, a total of 24 SWEETs were identified using the Morus alba (Ma) genome. …”
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The response of fungal community on citrus fruit surface to the application of Metschnikowia citriensis by Hongyan Zhang, Shupei Wang, Lanhua Yi, Jian Ming, Kaifang Zeng

“…Meira and Meyerozyma levels rose in M. citriensis-treated fruits during storage, though M. citriensis itself did not dominate. Furthermore, functional analysis of the fungal community revealed a significant presence of pathogens on the citrus fruit surface. …”
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Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae) by Qingfang Hou, Qingfang Hou, Linlin Yuan, Linlin Yuan, Haifeng Jin, Haifeng Jin, Han Yan, Han Yan, Fen Li, Fen Li, Shaoying Wu, Shaoying Wu

“…Ourfindings will be beneficial for improving the accuracy of qRT-PCR analysis for future functional analysis of the target gene expression in M. usitatus.…”
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Mitochondrial functional impairment in ARL3‐mutation related rod‐cone dystrophy by Xiaoli Zhang, Shun Yao, Lujia Zhang, Beisi Zhang, Mingzhu Yang, Qingge Guo, Jin Xu, Zhongfeng Wang, Bo Lei, Xiuxiu Jin

“…Our study demonstrated that ARL3‐related mutations play a significant role in the diversity of mitochondrial function, providing novel insights into the functional analysis of ARL3‐related mutations.…”
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Exploration of fecal microbiota in newly diagnosed patients with inflammatory bowel disease using shotgun metagenomics by Macarena Orejudo, Manuel J. Gómez, Sabino Riestra, Montserrat Rivero, Ana Gutiérrez, Iago Rodríguez-Lago, Luis Fernández-Salazar, Daniel Ceballos, José Manuel Benítez, Mariam Aguas, Iria Bastón-Rey, Fernando Bermejo, María José Casanova, Rufo H. Lorente-Poyatos, Yolanda Ber, Daniel Ginard, María Esteve, Ruth de Francisco, María José García, Rubén Francés, Rubén Francés, Ainhoa Rodríguez, Noelia Alcaide Suárez, Elena Guerra del Río, Pilar Soto, Pilar Nos, Manuel Barreiro-de Acosta, Iván Guerra, Daniel Hervías Cruz, Manuel Domínguez Cajal, Vanesa Royo, Montserrat Aceituno, Laila Aldars-García, Ana Garre, Cristina Ramírez, Irene Soleto, Ina Schuppe-Koistinen, Lars Engstrand, Montse Baldán-Martín, Fátima Sánchez-Cabo, Javier P. Gisbert, María Chaparro

“…However, the most significant differences in these patients compared to HC were observed in less abundant species, such as Toxoplasma gondii, Gemella morbillorum, and several species of the Adlercreutzia genera. Functional analysis in these patients highlighted changes in carbohydrate and nucleotide pathways.DiscussionOur data suggest that newly diagnosed CD patients show significant microbiota composition disparities compared to UC patients and HC. …”
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Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila by Malene L Urbanus, Andrew T Quaile, Peter J Stogios, Mariya Morar, Chitong Rao, Rosa Di Leo, Elena Evdokimova, Mandy Lam, Christina Oatway, Marianne E Cuff, Jerzy Osipiuk, Karolina Michalska, Boguslaw P Nocek, Mikko Taipale, Alexei Savchenko, Alexander W Ensminger

“…Through detailed structural and functional analysis, we show that metaeffector activity derives from a diverse range of mechanisms, shapes evolution, and can be used to reveal important aspects of each cognate effector's function. …”
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Genome-Wide Identification and Comprehensive Characterization of Luffa Sucrose Phosphate Synthase Gene Family and Revealing <i>LaSPS3/4</i>’s Role in Drought Resistance by Xiaocheng Tian, Jianting Liu, Guoliang He, Fei Yan, Hanyi Wang, Liujing Huang, Yeqiu Yin, Shaolong Sun, Chongjian Ma, Guoping Wang, Haisheng Zhu, Hongbo Zhao

“…Here, we present the first genome-wide identification and functional analysis of the <i>LaSPSs</i> in luffa. We identified nine <i>LaSPS</i> genes, characterized their physicochemical and evolutionary properties, and analyzed their expression patterns in different tissues and response to ethylene and drought treatments. …”
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Noninvasive anatomical assessment for ruling out hemodynamically relevant coronary artery anomalies in adults – A comparison of coronary-CT to invasive coronary angiography: The NA... by Marius R. Bigler, Anselm W. Stark, Isaac Shiri, Joel Illi, Matthias Siepe, Federico Caobelli, Andreas A. Giannopoulos, Ronny R. Buechel, Andreas Haeberlin, Dominik Obrist, Lorenz Räber, Christoph Gräni

“…As the sole presence of AAOCA does not always imply a revascularization, a detailed anatomical and functional analysis is crucial for clinical decision-making. …”
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Genome-wide analysis and identification of nuclear factor Y gene family in switchgrass (Panicum virgatum L.) by Hadia Hussain, Noor Fatima, Muhammad Sajid, Iqra Mehar, Maryam Noor, Kotb A. Attia, Yaser M. Hafez, Khaled Abdelaal, Tawaf Ali Shah

“…In conclusion, the findings of this study could provide a foundation for further cloning and functional analysis of NF-Y genes in switchgrass.…”
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Transcriptomics of HERVs reveals clinico-biological characterization of LTR5_Hs and HERVS71 loci in gastric cancer by Juan Xu, Jie Liu, Yanhuan Wang, Linjie Peng, Wang Li, Likai Ji, Yang Yang, Benshuai You, Yan Huang, Shikun Fang, Ping Ni, Wen Zhang, Chenglin Zhou

“…Differential and functional analysis indicated that these HERV elements significantly impacted the cell cycle, with their upregulation linked to tumor growth both in vitro and in vivo. …”
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Vaginal flora in HPV infection: a cross‑sectional analysis by Peining Dou, Feng Fang, Rongqiu Qin, Jingsong Nie, Xiaoyan Chen, Xiaoyan Yin, Yiding Wang, Shengying Shi

“…Statistically significant differences were observed in Nugent scores and bacterial vaginosis between the two groups (P < .05). In functional analysis, 11 proteins and 13 enzymes were found to be significantly altered in the HPV + group.Conclusion Our study demonstrates that disruptions in the vaginal flora are associated with HPV infection. …”
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Analysis of the Transcriptional Control of <i>Bcl11b</i> in Chicken: IRF1 and GATA1 as Negative Regulators by Lingling Qiu, Haojie Wang, Wenhao Li, Ting Yang, Hao Bai, Guobin Chang

“…Mutational and functional analysis further revealed interferon regulatory factor-1 (IRF1) and GATA-binding protein 1 (GATA1) as critical factors for the repression of chicken <i>Bcl11b</i>, thereby affecting cell apoptosis and ALV-J replication. …”
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Homeostatic signals, including IL-7 and self-MHC recognition, induce the development of peripheral helper T cells, which are enriched in the joints of rheumatoid arthritis by Ryosuke Tsurui, Hisakata Yamada, Takahiro Natori, Motoki Yoshimura, Yukio Akasaki, Shinya Kawahara, Hiroaki Niiro, Yuya Kunisaki, Yasuharu Nakashima

“…Development of Tph-like cells was assessed by flow cytometry, gene expression, and functional analysis. Chemotaxis of the Tph-like cells to RA synovial fluid (RASF) and the effect of RASF on the development of Tph-like cells was examined. …”
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Correlation between Genetic Variations and Serum Level of Interleukin 28B with Virus Genotypes and Disease Progression in Chronic Hepatitis C Virus Infection by Ahmed Al-Qahtani, Mashael Al-Anazi, Ayman A. Abdo, Faisal M. Sanai, Waleed Al-Hamoudi, Khalid A. Alswat, Hamad I. Al-Ashgar, Mohammed Q. Khan, Ali Albenmousa, Nisreen Khalaf, Nisha Viswan, Mohammed N. Al-Ahdal

“…The variation of IL28B genetic polymorphisms was found to be strongly associated with HCV infection when healthy control group was compared to HCV-infected patients with all P values <0.0001. Functional analysis revealed that subjects carrying rs8099917-GG genotype had higher serum level of IL28B than those with GT or TT genotypes (P=0.04). …”
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Perinatal Inflammation Results in Sex-Dependent Cardiac Dysfunction by Leeann R. Pavlek, Kathryn M. Heyob, Nitya R. Jacob, Saichidroopi Korada, Zahra Khuhro, Aiman Q. Khan, Terri A. Shaffer, Sara Conroy, Markus Velten, Lynette K. Rogers

“…Methods: Studies using our murine model of maternal inflammation (LPS) and neonatal hyperoxia exposure (O₂) observed pathological changes in cardiac structural proteins and functional analysis with sex dependent differences in pathologies at 10 months of age. …”
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Inclusion of Hybrid <i>Pennisetum</i> and Probiotics Enhanced Anaerobic Fermentation Quality and Bacterial Diversity of Alfalfa Silage by Jiaqi Tang, Jue Li, Xuelin Han, Jie Qiao, Shuangshuang Bu, Xiaoping Xie, Xin Li, Jing Zhou, Fulin Yang

“…LB alone and the combined inoculation also increased the relative abundance of <i>Lactobacillus</i> during the pre-silage period. Functional analysis through the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed considerable variations among the different probiotic treatments. …”
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Co-Inoculating <i>Burkholderia vietnamiensis</i> B418 and <i>Trichoderma harzianum</i> T11W Reduced <i>Meloidogyne incognita</i> Infestation of Tomato Plants by Yanqing Jiang, Wenzhe Li, Jishun Li, Jindong Hu, Yanli Wei, Yilian Wang, Hetong Yang, Yi Zhou, Yuanzheng Wu, Shanshan Zhang

“…Actinomycetota (46.42%) and Ascomycota (97.92%) were enriched in the co-inoculated rhizosphere, showing negative correlations with RKNs severity. Functional analysis indicates enriched metabolic pathways, including streptomycin and unsaturated fatty acid biosynthesis, enhancing microbial antagonism. …”
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Identification and characterization of variants in PSEN1, PSEN2, and APP genes in Chinese patients with early-onset Alzheimer's disease by Haitian Nan, Min Chu, Deming Jiang, Wenping Liang, Yu Li, Yiming Wu, Zhe Wang, Liyong Wu

“…Our study aimed to identify the genotypic and phenotypic spectrums in a Chinese EOAD cohort and confirm their pathogenicity by functional analysis. This study included 304 unrelated clinically diagnosed EOAD participants of Chinese Han ancestry. …”
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