The complete genome sequences of Bacillus velezensis B26: a promising biocontrol agent and biofertilizer [version 1; peer review: 1 approved, 2 approved with reservations] by Sandeep Mallya, Kiran Kumar Kolathur, Subrahmanyam Volety Mallikarjuna, Venkatesh Kamath B

“…Genome annotation identified 3776 protein-coding genes, 1 rRNA gene, 50 tRNA genes, 5 ncRNA genes, and 59 pseudogenes. Functional analysis of the B. velezensis B26 genome revealed 216 genes involved in carbohydrate metabolism, 3 genes in potassium metabolism, 148 genes linked for cofactors, vitamins, prosthetic groups and pigments, 10 genes involved in phosphorus metabolism, 24 genes associated with iron acquisition and metabolism, 20 genes for nitrogen metabolism, 6 genes involved in sulfur metabolism, 6 genes in secondary metabolism, 12 genes associated with metabolism of aromatic compounds, 43 genes involved in stress response and 36 genes associated with virulence, disease and defence. …”
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Changes in potential pathogenicity-associated proteins of Helicobacter cinaedi upon infection of macrophage cells by A. K. Voronina, B. A. Efimov, B. A. Efimov, M. V. Malakhova, P. V. Shnaider, O. M. Ivanova, M. Bogomiakova, V. O. Shender, V. O. Shender, M. A. Lagarkova, G. P. Arapidi, G. P. Arapidi, G. P. Arapidi

“…This study represents the first comprehensive analysis of the bacterium’s proteome under standard culture conditions and after infection of M1-type macrophage cells.ResultsWe identified 1,575 proteins in the H. cinaedi proteome, 109 of which were differentially upregulated after macrophage infection. Functional analysis revealed roles for these proteins in immune evasion, intracellular survival, and potential pathogenicity. …”
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Atomic-level investigation of KCNJ2 mutations associated with ventricular arrhythmic syndrome phenotypes by Saba Munawar, Corey L. Anderson, Louise Reilly, Ryan Woltz, Yusra Sajid Kiani, Nipavan Chiamvimonvat, Lee L. Eckhardt

“…Here, we combine in-depth and complementary computational molecular modeling techniques with functional analysis from three patients with ATS that harbor KCNJ2 mutations R67Q, R218L, and G300D. …”
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Designing an Electronic Marketing Model Based on Social Networks –Case Study on Sports Equipment Manufacturing Companies by Ziba Mohammad Rezaee, Korosh Veisi, Adel Fatemi, Samira Aliabadi, Mohsen Smaeili

“…Therefore, this study aimed to present an electronic marketing model based on social networks in sports equipment manufacturing companies.Methodology: This research was conducted qualitatively using a structural-functional analysis approach at the strategic level. The participants consisted of experts in sports marketing, media, and sports equipment production, and a snowball sampling method based on theoretical saturation was used to select 18 of them. …”
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Fecal microbiota landscape of commercial poultry farms in Faisalabad, Pakistan: A 16S rRNA gene-based metagenomics study by Muhammad Moman Khan, Muhammad Ahmed Mushtaq, Muhammad Suleman, Umer Ahmed, Muhammad Faisal Ashraf, Rizwan Aslam, Mashkoor Mohsin, Stefan Rödiger, Yasra Sarwar, Peter Schierack, Aamir Ali

“…Co-occurrence analyses revealed close interactions among phyla (Actinobacteriota, Proteobacteria, Firmicutes, Fusobacteriota, and Bacteroidota) and genera (Lactobacillus, Brevibacterium, Enterococcus), suggesting their pivotal roles within the microbial community. Additionally, functional analysis detected important metabolic pathways and traced microbial signatures of key pathogenic bacteria, enhancing our understanding of microbial contributions to poultry health. …”
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The GDF11 represses the Stat3 signaling pathway, conferring metabolic, inflammatory, and oncogenic restrictions in cells derived from human hepatocellular carcinoma. by Melissa Sánchez-Rodríguez, Monserrat Gerardo-Ramírez, Arturo Simoni-Nieves, María Concepción Gutiérrez-Ruiz, Roxana U Miranda-Labra, Verónica Souza, Leticia Bucio-Ortiz, Alejandro Escobedo-Calvario, Luis E. Gomez-Quiroz

“…It is observed that GDF11 has a profound impact on highly oncogenic pathways, highlighting the Stat3 pathway, beta-catenin, and HIF-1 alpha, among others. Functional analysis revealed that GDF11 could reduce cholesterol and lipid metabolism in general, inflammation, drug resistance, and stemness capacity. …”
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Comprehensive Ubiquitome Analysis of <i>Nicotiana benthamiana</i> Leaves Infected with Tomato Brown Rugose Fruit Virus by Jiali Yang, Donghai Wang, Boshen Zhang, Mangle Chen, Jianping Chen, Fei Yan, Shaofei Rao

“…Furthermore, enrichment analysis indicated that ToBRFV infection induces an increase in the ubiquitination levels of proteins associated with ion transport, MAPK signaling pathways, and plant hormone signal transduction, while the ubiquitination levels of proteins related to carbon metabolism and secondary metabolite synthesis decreased. Functional analysis of the three differentially ubiquitinated proteins revealed that a RING/U-box superfamily protein negatively regulates ToBRFV infection. …”
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Comparative microbiomic analysis of fecal microbiota associated with abdominal fat in ducks by Yangyang Shen, Yuhang Li, Jing Xiao, Jiawei Li, Yongfei Wu, Yan Wu, Hongbo Tang, Xinyan Fang, Lei Wang, Yujie Gong, Hao Chen, Xueming Yan

“…The Streptococcus, Campylobacter, Helicobacter, Enterobacter, Gallibacterium, and Escherichia genera were significantly enriched in LAF. Microbial functional analysis indicated that the HAF fecal microbiota was mainly involved in carbohydrate, nucleotide, lipid, amino acid, terpenoids, polyketides, and xenobiotic metabolism. …”
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Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability by Wei Lv, Wei Lv, Shiyu Zhao, Shiyu Zhao, Yunqing Hou, Yunqing Hou, Qian Tong, Qian Tong, Yaxin Peng, Yaxin Peng, Jianan Li, Jianan Li, Zaiyan Xu, Zaiyan Xu, Bo Zuo, Bo Zuo, Bo Zuo, Bo Zuo, Bo Zuo, Bo Zuo

“…Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. …”
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Downregulation of ferroptosis-related Genes can regulate the invasion and migration of osteosarcoma cells by Rui Ding, Le Liao, Jiahui Chen, Jian Zhang, Shenghao Cai, Xinxin Miao, Tao Li, Jiangminghao Zhao, Qi Chen, Xigao Cheng, Jianjian Deng

“…A risk score was validated as an independent predictive factor for OS via multivariate Cox regression. Functional analysis shows that these FRGs affect the occurrence of ferroptosis by influencing the intracellular ROS levels and play a regulatory role in the proliferation, migration, and infiltration of OS cells. …”
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Positional distribution and conservation of major phosphorylated sites in the human kinome by Athira Perunelly Gopalakrishnan, Athira Perunelly Gopalakrishnan, Prathik Basthikoppa Shivamurthy, Mukhtar Ahmed, Samseera Ummar, Poornima Ramesh, Sonet Daniel Thomas, Sonet Daniel Thomas, Althaf Mahin, Althaf Mahin, Mahammad Nisar, Sowmya Soman, Yashwanth Subbannayya, Rajesh Raju, Rajesh Raju

“…Knowledge-based functional analysis and sequence conservation across the family/subfamily identified phosphorylated sites unique to specific kinases that could contribute to their unique functions. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…Colony formation assay, 5-Ethynyl-2’-deoxyuridine assay, flow cytometry, and transwell assays were conducted for cell functional analysis. A xenograft mouse model assay was performed to analyze tumor tumorigenesis in vivo. m6A RNA immunoprecipitation assay was used to analyze the association of METTL3 and DLG2. …”
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Structural and Functional Characterization of the Most Frequent Pathogenic PRKN Substitution p.R275W by Bernardo A. Bustillos, Liam T. Cocker, Mathew A. Coban, Caleb A. Weber, Jenny M. Bredenberg, Paige K. Boneski, Joanna Siuda, Jaroslaw Slawek, Andreas Puschmann, Derek P. Narendra, Neill R. Graff-Radford, Zbigniew K. Wszolek, Dennis W. Dickson, Owen A. Ross, Thomas R. Caulfield, Wolfdieter Springer, Fabienne C. Fiesel

“…Immunoblot analysis and immunofluorescence were used to study mitophagy activation, and mitophagy execution was analyzed by flow cytometry of the reporter mitoKeima. The functional analysis was accompanied by structural investigation of PRKN p.R275W. …”
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METTL1-mediated m7G tRNA modification drives papillary thyroid cancer progression and metastasis by regulating the codon-specific translation of TNF-α by Weiwei Li, Ruiwang Xie, Huaying Chen, Junyu Lin, Minjie Zhong, Junsi Zhang, Shengkai Zheng, Cen Jiang, Xiangjin Chen, Sunwang Xu

“…Here, we found that METTL1 is significantly upregulated in PTC tissues compared to normal control tissues and is associated with poor PTC prognosis. Functional analysis confirmed that METTL1 promotes the proliferation and metastasis of PTC cells in a manner dependent on its tRNA methyltransferase activity. …”
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Development of a method of comparative analysis based on consumer properties of polymer compositions illustrated by adhesive plates by M. A. Mandrik, S. A. Albagachiev, L. A. Korol, E. A. Shemetova, I. I. Krasnyuk, G. V. Ramenskaya, I. A. Sadkovskii

“…To achieve the research goal, a set of methods was used, including bibliographic, analytical, comparative, survey and structural-functional analysis. To determine the weighting coefficients of various consumer characteristics of adhesive plates, a survey of experts was conducted.Results and discussion. …”
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Genome-wide characterization of the BBX gene family in perennial ryegrass and functional insights into the potentiality of LpBBX3 in drought and salt tolerance by Qingchun Liu, Yi Song, Yanfeng Liu, Xiao Xu, An Shao, Meng Wei, Yang He, Xinpei Zhang, Wei Wang

“…Transcriptome analysis showed that LpBBXs exhibited diverse expression patterns in different tissues and responded positively to drought stress. Finally, functional analysis revealed that LpBBX3 possessed transcriptional activation activity and was localized in the nucleus. …”
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Early diagnosed Zaki syndrome: identification of two novel WLS variants and a literature review by Anran Tian, Sujuan Li, Furong Liang, Minglan Yao, Niusha Mostafavi, Yingying Li, Xiaoping Luo, Cai Zhang

“…Genetic testing and functional analysis were conducted to confirm the diagnosis of Zaki syndrome. …”
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Gut microbiota and quantitative traits divergence at different altitude of long-tailed dwarf hamsters, Cricetulus longicaudatus by Yue Ren, Mengfan Tao, Xiaoliang Wang, Xinsheng Pu, Guangtong Guo, Kuiyou Chen, Bingyu Zhao, Yu Hou, Xin'gen Yang, Yumei Xu

“…And Providencia had significant correlation with body size. Finally, functional analysis of the gut microbiota showed changes in metabolic function that depended on elevation, and collinear network analysis showed how the gut microbiota interacts with each other. …”
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Review of Offshore Superconducting Wind Power Generation for Hydrogen Production by Cheng Zhang, Liufei Shen, Xingzheng Wu, Feiyue Shan, Long Chen, Shuai Liu, Zhiqiang Zheng, Litong Zhu, Jinduo Wang, Yujia Zhai

“…The main contribution of this research is the development of an innovative conceptual framework for a superconducting offshore wind-to-hydrogen energy system, where a small amount of liquid hydrogen is used to provide a deep-cooling environment for the HTS wind turbine and the remaining liquid hydrogen is used for the synthesis of ammonia as a final product. Through functional analysis, this study demonstrates its potential for enabling large-scale offshore hydrogen production and storage. …”
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Functional Correlates of Action Observation of Gait in Patients with Parkinson’s Disease by Giulia Bommarito, Martina Putzolu, Laura Avanzino, Carola Cosentino, Alessandro Botta, Roberta Marchese, Matilde Inglese, Elisa Pelosin

“…Differences between patients with PD (FOG+ and FOG-) and HS were assessed at the level of behavioral and functional analysis. Results. Gait parameters, including gait velocity, stride length, and their coefficients of variability (CV), were different in PD patients compared to HS, whereas gait performance was similar between FOG+ and FOG-. …”
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