Sequences within and upstream of the mouse Ets1 gene drive high level expression in B cells, but are not sufficient for consistent expression in T cells. by Alyssa Kearly, Prontip Saelee, Jonathan Bard, Satrajit Sinha, Anne Satterthwaite, Lee Ann Garrett-Sinha

“…Interestingly, functional analysis of several putative Ets1 regulatory elements using luciferase constructs suggested a high level of functional redundancy. …”
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Oxidative Phosphorylation-Related Signature Participates in Cancer Development, and PTPRG Overexpression Suppresses the Cancer Progression in Clear Cell Renal Cell Carcinoma by Liang Huang, Yu Xie, Weiqing Han, Shusuan Jiang, Lingfeng Zeng

“…Subsequently, we performed weighted gene coexpression network analysis to select cluster-related genes and performed functional analysis for them. The cluster-related genes were adopted to construct a risk score and nomogram for predicting patient prognosis with predictive accuracy evaluated. …”
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Engrafted human induced pluripotent stem cell-derived anterior specified neural progenitors protect the rat crushed optic nerve. by Leila Satarian, Mohammad Javan, Sahar Kiani, Maryam Hajikaram, Javad Mirnajafi-Zadeh, Hossein Baharvand

“…The hiPSC-NPs were labeled by green fluorescent protein or a fluorescent tracer 1,1' -dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) and injected two days after induction of ONC in hooded rats. Functional analysis according to visual evoked potential recordings showed significant amplitude recovery in animals transplanted with hiPSC-NPs. …”
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SNPs in inflammatory genes CCL11, CCL4 and MEFV in a fibromyalgia family study. by Zhifang Zhang, Jinong Feng, Allen Mao, Keith Le, Deirdre La Placa, Xiwei Wu, Jeffrey Longmate, Claudia Marek, R Paul St Amand, Susan L Neuhausen, John E Shively

“…<h4>Methods and findings</h4>Exome sequence analysis of 100 FM probands was performed at 17p13.3-q25 followed by functional analysis of SNPs found in the chemokine gene locus. …”
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Analysis of the main provisions and features of Darwinism from logical and epistemological positions by E.V. Degtyarev

“…To achieve this goal, the following methodological apparatus is used, first of all: firstly, the method of structural and functional analysis, and secondly, the methodological approach, which we define as «antifideism», consisting in the inadmissibil¬ity (as far as possible) of constructing rational (including scientific) reasoning on faith, no matter how authoritative it may seem, contrary to facts, logic and reason. …”
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Copper’s new role in cancer: how cuproptosis-related genes could revolutionize glioma treatment by Yu Wang, Sen Qiao, Ping Wang, Mi Li, Xiaozhen Ma, Hongmei Wang, Junhong Dong

“…The assessment of tumor mutational burden indicated that CRGs could serve as biomarkers for predicting the efficacy of immunotherapy in glioma. Further functional analysis of SLC31A1 showed that its elevation was associated with increased glioma cell malignancy, promoting proliferation and migration. …”
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Generalized gingivitis-related salivary proteomic profile in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry by Laura Teodoro de MARCHI, Amanda Borges PIRONDI, Talita Mendes Oliveira VENTURA, Larissa Tercilia GRIZZO, Marília Afonso Rabelo BUZALAF, Gerson Aparecido FORATORI-JUNIOR

“…Key biological processes included antimicrobial humoral response and hydrogen peroxide catabolism, with proteins linked to immune function and endopeptidase regulation. Functional analysis showed that Lactotransferrin (5-fold increase in G1), Haptoglobin (4-fold), and Immunoglobulin J chain (3-fold) were up-regulated, whereas Statherin (5-fold) and Protein S100-A8 (4-fold) were downregulated in G1. …”
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Porcine Ovarian piRNA Dynamics: A Comparative Study During Follicular Atresia by Jinbi Zhang, Long Huang, Xinxin Qin, Wenjie Li, Xiaolong Cheng, Zengxiang Pan

“…The target genes of differentially expressed piRNAs were predicted and subjected to functional analysis. A total of 452 piRNAs were identified across all samples, with 103 showing differential expression between HFs and AFs. …”
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Secondary metabolite biosynthetic gene clusters and microbial diversity as predicted by shotgun metagenomic sequencing approach in hospitals and pharmaceutical industry wastes by Niguse Kelile Lema, Mesfin Tafesse Gemeda, Adugna Abdi Woldesemayat

“…Across all samples, Pedobacter and Pseudomonas were consistently the most dominant bacterial genera. Functional analysis using gene ontology (GO) annotations highlighted the predominance of metabolic and biosynthetic processes. …”
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Dirichlet <i>μ</i>-Parametric Differential Problem with Multivalued Reaction Term by Mina Ghasemi, Calogero Vetro, Zhenfeng Zhang

“…We focus on the well-posedness of the problem in variable exponent Sobolev spaces, starting with energy functional analysis. Using a Galerkin approach with a priori estimate and embedding results, we show that the functional associated with the problem is coercive; hence, we prove the existence of generalized and weak solutions.…”
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Highly sensitive live-cell imaging-based cytotoxicity assay enables functional validation of rare epitope-specific CTLs by Kathrin Wellach, Kathrin Wellach, Kathrin Wellach, Angelika B. Riemer, Angelika B. Riemer

“…Here, we present a highly sensitive image-based cytotoxicity assay that allows the functional analysis of rare epitope-specific T cells. The live-cell imaging-based setup combines transient red labeling of target cells with a green caspase 3/7 probe, allowing reliable measurement of the fraction of apoptotic target cells. …”
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Transcriptome landscape of the adrenal gland and superior cervical ganglion from hypertension-induced left ventricular hypertrophy rat model by Chaohong Li, Fengting Liu, Xingyu Li, Xiaohua Yang, Qiaoqiao Li, Jing Huang

“…Results We identified 671 differentially expressed genes (DEGs) in the left ventricle, 1596 DEGs in the AG, and 343 DEGs in the SCG between SHR and WKY rats. Functional analysis revealed that these DEGs were involved in pathways related to immune inflammation, cellular senescence, metabolic responses, and synaptic transmission. …”
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Assessment of Terrestrial Carbon Sinks in China Simulated by Multiple Vegetation Models by Weiyi Xu, Jing Liu, Longgao Chen, Suchen Ying

“…This comprehensive evaluation of the simulation performance of DGVMs in China is anticipated to serve as an important reference for the functional analysis and parameter optimization of DGVMs within China.…”
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Atelocollagen Injection Enhances the Healing of Nonoperatively Treated Achilles Tendon Tears by Jaehwang Song MD, PhD, Chan Kang MD, PhD, Seongkyeong Jo MS

“…The flowchart of the experiment groups and surgical procedures involved in this study. 2. Functional analysis. 3. Biomechanical testing. 4. Histological analysis. 5. …”
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Alteration of the Rhizosphere Microbiota and Growth Performance of Barley Infected with <i>Fusarium graminearum</i> and Screening of an Antagonistic Bacterial Strain (<i>Bacillus a... by Yang Fu, Jing Luan, Jialei Shi, Wenzhu Tang, Xianzhen Li, Zhimin Yu, Fan Yang

“…Growth indicators (root length, plant height, fresh/dry mass) also exhibited that Baudin barley showed stronger resistance. Functional analysis underscored that the microbial community composition of Baudin barley promoted metabolic pathways related to plant resilience and was associated with improved seedling health. …”
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Shared hub genes in membranous nephropathy and kidney renal clear cell carcinoma: investigating molecular overlap and tumor progression by Peng Hui, Zhang Shuwen

“…Higher FYN and LGALS8 expression were linked to poorer survival. miRNA downregulation was validated in KIRC cell lines. Functional analysis revealed that FYN and LGALS8 promote KIRC progression through the ErbB signaling pathway, and knockdown experiments reduced cell proliferation, migration, and colony formation. …”
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Bone remodeling in patients with type I and type II diabetes mellitus in pre- and postmenopausal periods by S. S. Safarova, S. S. Safarova

“…Aim: to perform structural and functional analysis of the activity of reparative osteogenesis processes at pre- and postmenopausal women with diabetes mellitus (DM).Materials and Methods. …”
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Conservation of the dehiscence zone gene regulatory network in dicots and the role of the SEEDSTICK ortholog of California poppy (Eschscholzia californica) in fruit development by Dominik Lotz, Le Han Rössner, Katrin Ehlers, Doudou Kong, Clemens Rössner, Oliver Rupp, Annette Becker

“…Expression analysis of STK orthologs from dry dehiscing fruits of legumes (Vicia faba, Glycine max and Pisum sativum) shows their involvement in fruit development. Functional analysis using Virus-Induced Gene Silencing (VIGS) showed premature rupture of fruits and clarified the roles of EscaSTK: an evolutionary conserved role in seed filling and seed coat development, and a novel role in restricting cell divisions in the inner cell layer of the valve. …”
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Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression by Asif Rashid, Hiu Ling Fung, Alexander Hin Ning Tang

“…This activated GSK-3β phosphorylates β-catenin at Ser33/37 with a subsequently reduced β-catenin level in PCa cells. Our functional analysis revealed that SLC4A4 knockdown retards tumor growth and lowers invasion and migration potential. …”
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Integrated bioinformatics analysis identifies neutrophils and villous cytotrophoblasts infiltration characterized by BLC6 upregulation as associated with the co-occurrence of gesta... by Jingjing Xie, Le He, Shuang Qin

“…The common DEGs were predicted for functional analysis through GO and KEGG analysis. A protein-protein interaction (PPI) network was constructed to determine the common hub genes for GDM and PE. …”
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