Further characterisation of the translational termination-reinitiation signal of the influenza B virus segment 7 RNA. by Michael L Powell, Kendra E Leigh, Tuija A A Pöyry, Richard J Jackson, T David K Brown, Ian Brierley

“…Here, we provide further evidence for a direct interaction between mRNA and rRNA using antisense oligonucleotide targeting and functional analysis in yeast cells. The TURBS also binds initiation factor eIF3 and we show here that this protein stimulates reinitiation from both wild-type and defective TURBS when added exogenously, perhaps by stabilising ribosome-mRNA interactions. …”
Get full text

Norovirus regulation of the innate immune response and apoptosis occurs via the product of the alternative open reading frame 4. by Nora McFadden, Dalan Bailey, Guia Carrara, Alicia Benson, Yasmin Chaudhry, Amita Shortland, Jonathan Heeney, Felix Yarovinsky, Peter Simmonds, Andrew Macdonald, Ian Goodfellow

“…Using reverse genetics we demonstrated that expression of ORF4 is not required for virus replication in tissue culture but its loss results in a fitness cost since viruses lacking the ability to express ORF4 restore expression upon repeated passage in tissue culture. Functional analysis indicated that the protein produced from ORF4 antagonizes the innate immune response to infection by delaying the upregulation of a number of cellular genes activated by the innate pathway, including IFN-Beta. …”
Get full text

Adapting High-Resolution Respirometry to Glucose-Limited Steady State Mycelium of the Filamentous Fungus Penicillium ochrochloron: Method Development and Standardisation. by Christoph W Schinagl, Pamela Vrabl, Wolfgang Burgstaller

“…The activity of these alternative respiratory enzymes strongly depends on environmental conditions. Functional analysis of fungal ETS under highly standardised conditions for cultivation, sample processing and respirometric assay are still lacking. …”
Get full text

A functional variant at a prostate cancer predisposition locus at 8q24 is associated with PVT1 expression. by Kerstin B Meyer, Ana-Teresa Maia, Martin O'Reilly, Maya Ghoussaini, Radhika Prathalingam, Patricia Porter-Gill, Stefan Ambs, Ludmila Prokunina-Olsson, Jason Carroll, Bruce A J Ponder

“…We used the mapping of DNase I hypersensitive sites as a tool to prioritise regions for further functional analysis. This approach identified rs378854, which is in complete linkage disequilibrium (LD) with rs620861, as a novel functional prostate cancer-specific genetic variant. …”
Get full text

MBD3 localizes at promoters, gene bodies and enhancers of active genes. by Takashi Shimbo, Ying Du, Sara A Grimm, Archana Dhasarathy, Deepak Mav, Ruchir R Shah, Huidong Shi, Paul A Wade

“…MBD3 enrichment was also noted at promoters modified by H3K27me3. Functional analysis of chromatin indicated that MBD3 regulates nucleosome occupancy near promoters and in gene bodies. …”
Get full text

Development and validation of a four‐lipid metabolism gene signature for diagnosis of pancreatic cancer by Yanrong Ye, Zhe Chen, Yun Shen, Yan Qin, Hao Wang

“…Survival analysis data showed that the model could stratify the prognosis of patients with different clinical characteristics, and the model has clinical independence. Functional analysis indicated that the model is associated with multiple cancer‐related pathways. …”
Get full text

Bioinformatics Analysis Identifies TNFRSF1A as a Biomarker of Liver Injury in Sepsis TNFRSF1A is a Biomarker for Septic Liver Injury by Shangxun Zhou, Wei Zhao, Junjie Li, Yang Huang, Jing Yang, Qianmei Wang, Yunyun Xu, Chujun Duan, Yutong Wang, Wen Yin

“…The association of these DEGs with infection and liver disease was analyzed by using the CTD database. GO functional analysis, KEGG pathway enrichment analysis, and protein-protein interaction (PPI) network analysis were performed to elucidate the potential molecular mechanism of DEGs. …”
Get full text

Evaluation of the cervical liquid-based cytology sample as a microbiome resource for dual diagnosis. by Chiwoong Lim, Young-Jun Seo, Ji-Yeong Lee, Eun Sung Jung, Sunyoung Lee, Hayoung Kim, Kidong Kim, Jun-Mo Kim

“…The analysis of differential abundance highlighted variations in microbial abundance among individuals, which were more prominent than those resulting from the sampling methods. Functional analysis identified arachidonic acid and alpha-linolenic acid metabolism, along with a cautionary note regarding the low mean proportion values. …”
Get full text

Wnt signaling is required for early development of zebrafish swimbladder. by Ao Yin, Svitlana Korzh, Cecilia L Winata, Vladimir Korzh, Zhiyuan Gong

“…<h4>Significance/conclusion</h4>We first provided a new set of gene markers for the three tissue layers of swimbladder in zebrafish and demonstrated the expression of several key genes of Wnt signaling pathway in developing swimbladder. Our functional analysis data indicated that Wnt/β-catenin signaling is required for swimbladder early development and we also provided evidence for the crosstalk between Wnt and Hh signaling in early swimbladder development.…”
Get full text

Psychological processes and abilities for ceasing sex as self-injury– a qualitative study by Cathrine Apelqvist, Tove Irmelid, Linda S. Jonsson, Cecilia Fredlund

“…Thematic analysis was used for the study, with the preunderstunding of cognitive behavioral therapy treatment and functional analysis. Results Five abilities were seen as important for cessation of SASI; (1) Revised core beliefs about the self which were achieved through new experiences or cognitive restructuring. (2) Evolved emotional competence achieved through understanding or acceptance of emotions or new coping skills. (3) Increased relational competence via new relationship experiences or new communication skills. (4) Acquired meta-perspective through insight and knowledge of SASI. (5) Strengthened psychological empowerment through new relationships to the body and sexuality, transfer of responsibility or norm-critical perspective. …”
Get full text

Diversity and Metabolic Potential of Gut Bacteria in <i>Dorcus hopei</i> (Coleoptera: Lucanidae): Influence of Fungus and Rotten Wood Diets by Pan Wang, Xiaoyan Bin, Xingjia Xiang, Xia Wan

“…Bacteroidetes were more abundant in rotten-wood-fed larvae (7.61%) than fungus-fed larvae (0.15%), while Proteobacteria were more abundant in fungus-fed larvae. Functional analysis revealed that rotten-wood-fed larvae were primarily related to carbohydrate and amino acid metabolism, whereas fungus-fed larvae exhibited enhanced membrane transport function. …”
Get full text

Heat Shock Protein 70 Genes Are Involved in the Thermal Tolerance of <i>Hippodamia variegata</i> by Qing Yang, Yanhui Lu

“…We found the largest number of differentially expressed genes (DEGs) in the 35 °C vs. 32 °C group (1151) followed by the 38 °C vs. 32 °C group (1054) and then the 38 °C vs. 35 °C group (901), indicating that <i>H. variegata</i> expressed the largest number of newly mobilized genes under medium-high temperature (35 °C). Gene functional analysis showed that a large number of DEGs were involved in “Catalytic activity”, “Oxidoreductase activity”, “Metabolic pathways”, and “Longevity regulating pathway-multiple species” gene groups. …”
Get full text

Development and characterization of an in vitro fluorescently tagged 3D bone-cartilage interface model by Mary Adams, Mary Adams, Jessica Cottrell

“…We used an Incucyte and functional analysis to assess the cells throughout the differentiation process. …”
Get full text

GRAS transcription factor- Genome-wide identification, characterization and expression analysis in Avena sativa L. under salinity stress by Muskan Bokolia, Nibha TV, Jangala Chandini Priya, Baljinder Singh

“…These GRAS genes represent promising candidates for in-depth functional analysis and can be leveraged to develop stress-resilient cultivars in Avena sativa, enhancing crop resilience and sustainability.…”
Get full text

MODELING THE IMPACT OF THE TRANSPORT PROVISION OPTION ON PROJECT RISKS by Svitlana Rusanova

“…The following methods are used: system analysis, functional analysis. Results: project risks are formed under the influence of many factors, and transport provision is one of the components of this set. …”
Get full text

Lipocalin-7 is a matricellular regulator of angiogenesis. by Leslie J Brown, Mariam Alawoki, Mary E Crawford, Tiffany Reida, Allison Sears, Tory Torma, Allan R Albig

“…<h4>Conclusions and significance</h4>No functional analysis has previously been performed to elucidate the function of LCN7 in vascular or other cellular processes. …”
Get full text

Longwall top coal caving technological process in the notation of the system-functional approach by Cherkasov P.V.

“…Based on the conducted system-functional analysis, a stand structure has been developed for measuring the volume of rock mass produced using machine vision and projection of a grid of laser lines, which makes it possible to measure the volume of rock mass in the boundary conditions of illumination, humidity and dustiness, and It is also necessary to determine the influence of environmental factors on the quality of recognition of a laser grid by a machine vision system…”
Get full text

Fat-tail allele-specific expression genes may affect fat deposition in tail of sheep. by Hossein Mansourizadeh, Mohammad Reza Bakhtiarizadeh, Luciana Correia de Almeida Regitano, Jennifer Jessica Bruscadin

“…Using a rigorous computational pipeline, 115 differential ASE genes were identified, which were narrowed down to four genes (LPL, SOD3, TCP1 and LRPAP1) for being detected in at least two studies. Functional analysis revealed that the ASE genes were mainly involved in fat metabolism. …”
Get full text

Comprehensive Analysis of Breast Cancer Cell Lines: Genome-wide Insights from ChIP-seq Analysis by Tanishq Sahu, Ruchi Yadav

“…Materials and Methods: Galaxy server (https://usegalaxy.org/) was used for complete ChIP-seq data analysis; different tools such as fast-quality control (QC), multi-QC, Bowtie2, model-based analysis of ChIP-sequencing, and ChIPseeker tools were used for motif enrichment and functional analysis. Motif analysis was done through the Multiple Expectation maximizations for Motif Elicitation database (https://meme-suite.org/meme/db/motifs). …”
Get full text

Technocratic belief or civil society: reflections on the typological concepts of sociology by A. V. Shcherbina

“…The comparative historical method, structural-functional analysis, and typological approach are applied in the present work.Results. …”
Get full text