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Establishment of an Efficient <i>Agrobacterium rhizogenes</i>-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit
Published 2025-07-01“…Finally, this system was applied for functional analysis using <i>PeMYB123</i>, which may be involved in proanthocyanidin accumulation. …”
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Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses
Published 2025-02-01“…Herein, we are the first to report a 5-year-old boy with the blended phenotypes of infantile hypotonia, severe neurodevelopmental disorder, patent ductus arteriosus, cryptorchidism, obesity, distinctive facial features, and elevated isovaleryl carnitine.MethodsTrio-based whole-exome sequencing was performed on genomic DNA from peripheral blood samples from the boy and his parents. Functional analysis of the IVD variant in vitro was performed. …”
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Pointwise Structure–Function Analysis of the Ellipsoid Zone in Retinitis Pigmentosa Using an Artificial Intelligence-Assisted OCT and Microperimetry Overlay
Published 2025-11-01“…Objective: To perform a pointwise structure–function analysis of the ellipsoid zone (EZ) in retinitis pigmentosa (RP) using an artificial intelligence–based overlay to understand EZ structure–function relationships. …”
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Qualitative Risk Assessment Methodology for Maritime Autonomous Surface Ships: Cognitive Model-Based Functional Analysis and Hazard Identification
Published 2025-05-01Subjects: Get full text
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Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis
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Functional analysis of a novel FBN1 deep intronic variant causing Marfan syndrome in a Chinese patient
Published 2025-03-01Get full text
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Functional Analysis of <i>NdBCO-like4</i> Gene in Pigmentation of <i>Neocaridina denticulata sinensis</i>
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Functional Analysis of <i>FoCrpA</i> in <i>Fusarium oxysporum</i> Causing Rice Seedling Blight
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PIK3R1 mutations in individuals with insulin resistance or growth retardation: Case series and in silico functional analysis
Published 2025-08-01“…We here describe four Japanese individuals from three families with SHORT syndrome who harbor either a common or a previously unknown mutation in PIK3R1 as well as provide an in silico functional analysis of the mutant proteins. Materials and Methods Gene sequencing was performed to identify PIK3R1 mutations. 3D structural analysis of wild‐type and mutant p85α proteins was performed by homology modeling, and structural optimization and molecular dynamics simulations confirmed stable trajectories. …”
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High-Quality Assembly of the Apple Fungal Pathogen <i>Marssonina coronaria</i> Genome and Functional Analysis of Candidate Effectors
Published 2025-05-01“…Based on genome annotation, 97 candidate effector proteins (CEPs) were identified, and 61 CEPs were successfully cloned for functional analysis. Transient expression assays in <i>Nicotiana benthamiana</i> revealed that eight CEPs significantly suppressed BAX-induced cell death, with McCEP12, McCEP23, McCEP24, and McCEP52 concurrently inhibiting flg22-triggered reactive oxygen species bursts. …”
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