Neuron-Specific Glycine Metabolism Links Transfer RNA Epitranscriptomic Regulation to Complex Behaviors by Jennifer Blaze, Viviana Dolores Evans, Jessica Abigail Feria Pliego, Petr Unichenko, Behnam Javidfar, Soeren Heissel, Hanan Alwaseem, Zachary Pennington, Denise Cai, Henrik Molina, Christian Henneberger, Schahram Akbarian

“…Changes in extracellular glycine levels were monitored by an optical glycine Förster resonance energy transfer (FRET) sensor in the hippocampus, and behavioral phenotyping included cognition, anxiety-like behavior, and behavioral despair. …”
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New approach to generating of human monoclonal antibodies specific to the proteolytic domain of botulinum neurotoxin A by Marina Vladimirovna Silkina, Alena Sergeevna Kartseva, Alena Konstantinovna Riabko, Mariia Aleksandrovna Makarova, Metkhun Madibronovich Rogozin, Yana Olegovna Romanenko, Igor Georgievich Shemyakin, Ivan Alekseevich Dyatlov, Victoria Valerievna Firstova

“…The 3 antibodies obtained were then purified by affinity chromatography, analyzed for binding by Western blot assay and neutralization by FRET assay. Results: We have succeeded in creating 3 hybridomas that secrete huMAbs specific to native BoNT/A and the proteolytic domain (LC) of BoNT/A. …”
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Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India by Sanjay K. Biswas, Mayank Mittal, Ekata Sinha, Vandana Singh, Nidhi Arela, Bharat Bajaj, Pramod K. Tiwari, Vishwa M. Katoch, Keshar K. Mohanty

“…The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for CCL2-2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples. …”
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