Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…The refined 2D PCR system was confirmed to be free from cross-reactions and exhibited high specificity, capable of detecting 12 target genes within a single tube. …”
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Immunoregulative coating for scarless healing in anterior cruciate ligament reconstruction by Shuang Wang, Chao Xu, Yuanman Yu, Jie Li, Tianwu Chen, Jing Wang, Changsheng Liu

“…To address this issue, we developed a multi-layered immune-regulating hydrogel coating for scar-free PET-bone integration. Comprising gelatin methacrylate (GelMA), polyethyleneglycol diacrylate (PEGDA), and sulfated polysaccharide (SCS), the hydrogel forms a hydrogen-bonded lubricating layer to reduce friction. …”
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Syntheses, crystal structures, Hirshfeld surface analyses and crystal voids of 1-(4-bromophenyl)-2,2-dichloroethan-1-one and 2,2-dibromo-1-(p-tolyl)ethan-1-one by Atash V. Gurbanov, Firudin I. Guseinov, Aida I. Samigullina, Tuncer Hökelek, Khudayar I. Hasanov, Tahir A. Javadzade, Alebel N. Belay

“…The volumes of the crystal voids and the percentages of free spaces in the unit cells were calculated to 111.55 Å3 and 12.27% for (I) and 63.37 Å and 6.69% for (II), showing that no large cavities are present in either structure.…”
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Impact of periodic neck ultrasonography on locoregional disease control in surveillance after total thyroidectomy for patients with low- and intermediate-risk papillary thyroid car... by Takahiro Inaishi, Dai Takeuchi, Takahiro Ichikawa, Gai Inaguma, Atsushi Hashizume, Masaki Okazaki, Norikazu Masuda, Toyone Kikumori

“…There was no significant difference in locoregional relapse-free survival between the PE and US groups (97.0 vs. 98.7%, p = 0.541). …”
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Combined Replacement of Fishmeal and Fish Oil by Poultry Byproduct Meal and Mixed Oil: Effects on the Growth Performance, Body Composition, and Muscle Quality of Tiger Puffer by Lili Zhao, Lin Li, Feiran Zhang, Peng Li, Yanlu Li, Jian Liu, Yuliang Wei, Mengqing Liang, Qiang Ma, Houguo Xu

“…Dietary PBM and MO had marginal effects on free amino acid composition and texture of fish muscle, but exerted complicated effects on the muscle volatile flavor compound composition. …”
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Genetics of Stay-Green Trait and Its Association with Leaf Spot Tolerance and Pod Yield in Groundnut by Rukiya Danful, Yussif Baba Kassim, Doris Kanvenaa Puozaa, Richard Oteng-Frimpong, Masawudu Abdul Rasheed, Alexander Wireko-Kena, Richard Akromah

“…Twenty-five advanced groundnut breeding lines with varying degrees of tolerance for leaf spot tolerance were evaluated under diseased and disease-free conditions, after which four were selected for genetic studies. …”
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Ethical issues raised in the care of the elderly during the SARS-CoV-2 pandemic and possible solutions for the future: a systematic review of qualitative scientific literature by Mohamed Amine Bouchlaghem, Zoé Estey-Amyot, Erika Ethier, Miruna Anohim, Marie-Laurence Ouellet-Pelletier, Lyse Langlois, Félix Pageau

“…We highly recommend that future research be undertaken to test the efficacy of the proposed solutions in providing age-friendly, dilemma-free health care and environments.…”
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A multi-stage weakly supervised design for spheroid segmentation to explore mesenchymal stem cell differentiation dynamics by Arash Shahbazpoor Shahbazi, Farzin Irandoost, Reza Mahdavian, Seyedehsamaneh Shojaeilangari, Abdollah Allahvardi, Hossein Naderi-Manesh

“…Therefore, we developed an innovative, weakly supervised model, aided by convolutional neural networks, to perform label-free spheroid segmentation. Since obtaining pixel-level ground truth labels through manual annotation is labor-intensive, our approach improves the overall quality of the ground-truth map by incorporating a multi-stage process within a weakly supervised learning framework. …”
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Fluid Origin and Evolution of the Roc Blanc Silver Deposit (Jebilet Massif, Variscan Belt, Morocco): Constraints from Geology and Fluid Inclusions by Amal El Arbaoui, Ismaïla N’Diaye, Zaineb Hajjar, Amina Wafik, Abdelhak Boutaleb, Said Ilmen, Abderrahim Essaifi, Mohammed Bouabdellah

“…In addition, fluid inclusion studies reveal that the mineralising fluid corresponds to a mixture of metamorphic fluid (H2O-CH4-N2-CO2) with surface to subsurface aqueous gas-free fluids (H2O-salt, meteoric, or brine).…”
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Microencapsulation of single-cell protein from various microalgae species by Purnama Sukardi, Tjahjo Winanto, , Hartoyo, Taufik Budhi Pramono, Eko Setyo Wibowo

“…The results showed that the highest value of nutrition content was Spirulina sp. and the average composition of protein, crude lipid, carbohydrate, ash, nitrogen free extract, and water content was 34.80%, 0.30%, 18.53%, 20.09%, 26.29%, and 13.32%, respectively. …”
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Functionalization of collagen fiber with nano-islands of silver via atomic layer deposition to promote bone healing by Sarah Hashemi Astaneh, Leonardo P. Faverani, Harshdeep Bhatia, Eduardo Dallazen, Monique Gonçalves Costa, Edilson Ervolino, Valentim A.R. Barão, Cortino Sukotjo, Christos G. Takoudis

“…In vivo assessment in rat model showed the biocompatibility of the Ag/Collagen, and greater new bone formation compared to control. This novel solvent-free method can be used to functionalize sensitive materials used in surgeries as bone grafting agents to enhance osteopromotive properties without any adverse effects to the cellular environment.…”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The plasmid remained stable for 8 days under non-selective conditions, and growth rates were comparable to non-bioluminescent parent strains in antibiotic-free conditions. However, growth was notably different in the presence of chloramphenicol, indicating successful plasmid retention and function. …”
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Synthesis of bioactive quinazolin-4(3H)-one derivatives via microwave activation tailored by phase-transfer catalysis by El-Badry Yaser A., El-Hashash Mahr A., Al-Ali Khalil

“…The reinvestigation of the key starting quinazolinone 2 was performed under microwave irradiation (MW) and solvent-free conditions. Combination of MW and phase-transfer catalysis using tetrabutylammonium benzoate (TBAB) as a novel neutral ionic catalyst was used for carrying out N-alkylation and condensation reactions of compound 2 as a simple, efficient and eco-friendly technique. …”
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How do families access holiday activities and food programmes and other support? Learnings from the Borough of Southwark holiday activities and food club’s evaluation by Lorraine McSweeney, Anita Sewornu, Louis Goffe, Bronia Arnott, Ashley Adamson

“…IntroductionThe school holidays can be a challenging time for many families especially for those reliant on free school meals. The Holiday Activities and Food (HAF) programme aims to provide disadvantaged families across England with healthy meals and enriching activities for children and young people. …”
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Sex differences in ventricular-vascular interactions associated with aerobic capacity by Barbara N. Morrison, Peter M. Mittermaier, Garth R. Lester, Michael E. Bodner, Anita T. Cote

“…Methods Sixty-eight males and females (38% females, 35 ± 10y) characterised as recreational exercisers to highly trained endurance athletes, and free of chronic disease underwent a cycle ergometer to assess VO2max. …”
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The Effects of Thyroid Hormone Levels on Patent Ductus Arteriosus Closure in Newborns by Basak KAYA, Hasan AKDUMAN, Dilek DILLI, Semra CETINKAYA, Ahmet OKTEM, Utku Arman ORUN, Mehmet TASAR, Aysegul ZENCIROGLU

“…Gestational week, birth weight, and mode of delivery were similar between the medical and surgical treatment groups (p>0.05). Free thyroxine levels were significantly lower in the MR-PDA group than in the ST-PDA group (p=0.01). …”
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HER2-related biomarkers predict clinical outcomes with trastuzumab deruxtecan treatment in patients with HER2-expressing metastatic colorectal cancer: biomarker analyses of DESTINY... by Salvatore Siena, Kanwal Raghav, Toshiki Masuishi, Kensei Yamaguchi, Tomohiro Nishina, Elena Elez, Javier Rodriguez, Ian Chau, Maria Di Bartolomeo, Hisato Kawakami, Fumitaka Suto, Makito Koga, Koichiro Inaki, Yusuke Kuwahara, Issey Takehara, Daniel Barrios, Kojiro Kobayashi, Axel Grothey, Takayuki Yoshino

“…Higher levels of HER2 biomarkers in baseline tissue and liquid biopsies, including HER2 status (IHC/ISH), HER2/CEP17 ratio, HER2 ISH signals, HER2 H-score, plasma HER2 (ERBB2) amplification status, HER2 adjusted plasma copy number, and HER2 extracellular domain correlate with antitumor activity (indicated by objective response rate, progression-free survival, and overall survival) of T-DXd. Baseline circulating tumor DNA (ctDNA) analysis suggests antitumor activity of T-DXd in patients who had baseline activating RAS, PIK3CA, or HER2 mutations detected in ctDNA.…”
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The Use of Machine Learning to Create a Risk Score to Predict Survival in Patients with Hepatocellular Carcinoma: A TCGA Cohort Analysis by Samer Tohme, Hamza O Yazdani, Amaan Rahman, Sanah Handu, Sidrah Khan, Tanner Wilson, David A Geller, Richard L Simmons, Michele Molinari, Christof Kaltenmeier

“…ANN analysis of the 75 most significant genes predicting disease-free survival (DFS) was performed. Next, CTA results were used for creation of the scoring system. …”
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Characterization of the core microflora and nutrient composition in packaged pasteurized milk products during storage by Ruixue Ding, Shanshan Yang, Lijuan Geng, Yumeng Liu, Baoping He, Liyun Liu, Xiqing Yue, Rina Wu, Junrui Wu

“…Moreover, the changes in nutritional composition of the pasteurized milk, including in 16 free amino acids, 7 taste values, and 8 chemical constituents, were analyzed using principal component and multi-factor analyses. …”
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An Ontology for Digital Medicine Outcomes: Development of the Digital Medicine Outcomes Value Set (DOVeS) by Benjamin Rosner, Matthew Horridge, Guillen Austria, Tiffany Lee, Andrew Auerbach

“…ObjectiveThis study aimed to develop, with inputs from industry, health care providers, payers, regulatory bodies, and patients through the Accelerated Digital Clinical Ecosystem (ADviCE) consortium, an ontology specific to DHT outcomes, the Digital medicine Outcomes Value Set (DOVeS), and to make this ontology publicly available and free to use. MethodsFrom a starting point of a 4-generation–deep hierarchical taxonomy developed by ADviCE, we developed DOVeS using the Web Ontology Language through the open-source ontology editor Protégé, and data from 185 vendors who had submitted structured product information to ADviCE. …”
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