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Inheritance patterns of male asexuality in hybrid males of a water frog Pelophylax esculentus
Published 2024-09-01“…To understand the mechanisms of gametogenesis in hybrids, we performed three-generation experiments of sexual P. ridibundus females and hybrids from all-male hybrid populations. Using fluorescent in situ hybridization, micronuclei analysis, flow cytometry and genotyping, we found that most adult hybrid males simultaneously produced two types of clonal sperm. …”
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Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes
Published 2023-01-01“…We also detected bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) in situ using immunofluorescence (IF) and western blotting and the 16S rRNA gene using fluorescent in situ hybridization (FISH). In the livers of six rats, we detected 54,867.50±6450.03 effective tags of the 16S rRNA gene and clustered them into 1003 kinds of operational taxonomic units (OTUs; 805.67±70.14, 729–893). …”
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23
Biological and economic characteristics of the allotetraploid with genomic formula DDAuAu from the cereal family
Published 2019-10-01“…Cytogenetic analysis using fluorescent in situ hybridization showed the presence of all chromosomes of the D genome in the chromosomal complement of the synthetic. …”
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The creation and characterization of the bread wheat line with a centric translocation T2DL.2RL
Published 2019-11-01“…The chromosomal composition of karyotypes was studied using fluorescent in situ hybridization and C-banding. Two centric translocations, derived from two long arms of chromosomes 2D and 2R, T2DL.2RL, were identified, the remaining 40 wheat chromosomes did not undergo modifications. …”
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25
Regions enriched for DNA repeats in chromosomes of Macrostomum mirumnovem, a species with a recent Whole Genome Duplication
Published 2020-10-01“…The generation of microdissected DNA libraries enriched for DNA repeats followed by DNA probe preparation and fluorescent in situ hybridization (FISH) were performed. The DNA probes obtained marked chromosome regions enriched for different DNA repeats in the M. mirumnovem chromosomes. …”
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Development of the genetic classification of Aegilops columnaris Zhuk. chromosomes based on the analysis of introgression lines Triticum aestivum×Ae. columnaris
Published 2017-04-01“…Altogether, five different chromosomes of Aegilops columnaris have been detected in the karyotypes of 15 lines by C-banding and fluorescent in-situ hybridization (FISH). Based on substitution spectra, these chromosomes were identified as 3Ае1, 3Ае2, 5Ае2, 6Ае1 and 6Ае2. …”
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Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species
Published 2017-01-01“…Genome comparisons were performed through interspecific fluorescent in situ hybridization (FISH) with total genomic DNAs of each species as probes (GISH). …”
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28
Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture
Published 2010-01-01“…Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs. …”
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New insights into interspecies relationships, chromosomal evolution, and hybrid identification in the Lycoris Herb.
Published 2025-01-01“…This study utilizes karyotype structure, genome size, and fluorescent in situ hybridization (FISH) technology to explore the chromosomal evolution and hybrid identification of Lycoris employing three approaches at the cytogenetic level. …”
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De Novo Acute Myeloid Leukemia with Combined CBFB-MYH11 and BCR-ABL1 Gene Rearrangements: A Case Report and Review of Literature
Published 2020-01-01“…Cytogenetic studies revealed two-related clones, a minor clone with inv(16) and a major clone with concurrent inv(16) and t(9;22) rearrangements. Fluorescent in situ hybridization studies confirmed these rearrangements. …”
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31
Hepatic and perihepatic PEComas: A study describing a series of five rare cases
Published 2024-04-01“…A comprehensive panel of immunohistochemical stains was used and fluorescence in-situ hybridization analysis was performed for the TFE3 gene using break-apart probes. …”
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Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue
Published 2010-01-01“…Background: The simultaneous detection of protein expression and gene copy number changes in patient samples, like paraffin-embedded tissue sections, is challenging since the procedures of immunohistochemistry (IHC) and Fluorescence in situ Hybridization (FISH) negatively influence each other which often results in suboptimal staining. …”
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33
Dedifferentiated Liposarcoma Arising in an Esophageal Polyp: A Case Report
Published 2012-01-01“…The diagnosis was confirmed by demonstration of CPM gene amplification by fluorescence in situ hybridization (FISH).…”
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Chromosome 22q11.2 Deletion Syndrome Presenting as Adult Onset Hypoparathyroidism: Clues to Diagnosis from Dysmorphic Facial Features
Published 2013-01-01“…The diagnosis was confirmed by fluorescence in situ hybridization, which found microdeletion in 22q11.2 region. …”
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Two Cases of Ectopic Hamartomatous Thymoma Masquerading as Sarcoma
Published 2017-01-01“…In these cases, fusion gene detection via polymerase chain reaction or fluorescence in situ hybridization was useful for differentiating EHT from synovial sarcoma. …”
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Osteosarcomatous Divergence in Dedifferentiated Liposarcoma Presenting as a Colonic Mass
Published 2019-01-01“…Immunohistochemical studies showed diffuse positivity for SATB2 in the atypical cells and fluorescence in situ hybridization revealed high-level amplification of MDM2 gene, supporting the diagnosis of well-differentiated and dedifferentiated liposarcoma with heterologous osteosarcomatous differentiation.…”
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Primary Cutaneous Follicle Centre Lymphoma with Hodgkin and Reed-Sternberg Like Cells: A Case Report and Review of the Literature
Published 2017-01-01“…The excisional biopsy of this skin mass was remarkable for involvement by a follicle centre cell derived lymphoma with a nodular and diffuse pattern associated with a subset of scattered Hodgkin and Reed-Sternberg like cells. Fluorescence in situ hybridization studies did not detect the presence of IgH-bcl2 fusion transcript, and molecular studies were negative for immunoglobulin heavy chain gene rearrangements and EBV DNA sequences. …”
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The Partial Duplication of the 5′ Segment of KMT2A Revealed KMT2A-MLLT10 Rearrangement in a Boy with Acute Myeloid Leukemia
Published 2017-01-01“…Here, we report on a boy who developed acute monocytic leukemia. Fluorescence in situ hybridization revealed the duplication of the 5′ segment with 2 normal alleles at KMT2A which was eventually found to be fused with MLLT10. …”
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Primary Pulmonary Synovial Sarcoma: A Case with Unique and Impressive Computed Tomography Findings
Published 2015-01-01“…A diagnosis of PPSS was made through the identification of an SS18 translocation by fluorescence in situ hybridization. She was started on adriamycin, ifosfamide and mesna chemotherapy. …”
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Molecular Cytogenetic Characterization of a Non-Robertsonian Dicentric Chromosome 14;19 Identified in a Girl with Short Stature and Amenorrhea
Published 2012-01-01“…Subsequent molecular cytogenetic analysis by fluorescence in situ hybridization (FISH) using whole chromosome paints, centromeric probes, as well as gene specific probes confirmed the dicentric nature of the derivative chromosome and indicated that the rearrangement involved the short arms of both of these chromosomes. …”
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