Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II in Trypanosoma brucei by Rudo Kieft, Laura Cliffe, Haidong Yan, Robert J. Schmitz, Stephen L. Hajduk, Robert Sabatini

“…Therefore, we show that epigenetic mechanisms exist to regulate gene expression via Pol II transcription initiation of gene clusters in a mono-allelic fashion. These findings suggest epigenetic chromatin-based regulation of gene expression is deeply conserved among eukaryotes, including early divergent eukaryotes that rely on polycistronic transcription.IMPORTANCEThe single-cell parasite Trypanosoma brucei causes lethal diseases in both humans and livestock. …”
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Rapid Verbal Persuasion to increase influenza vaccine uptake: protocol for a randomized hybrid type 2 effectiveness -implementation trial by Siyuan Liu, Lan Gao, Yingying Jin, Jiangyun Chen, Dadong Wu, Yiyuan Cai, Tao Wang, Sanhao Huang, Ciling Yan, Run Wang, Dong Roman Xu

“…Rapid Verbal Persuasion (RVP), with highly rapid fashion, has a strong evidence base in promoting behavior change. …”
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IŠ KO KYLA ŽMOGAUS TEISĖS? by Evaldas Nekrašas

“…Evaldas Nekrašas Summary The traditional understanding of human rights as divine or inborn is out of fashion today. Positivism succeeded in undermining it. …”
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Use of Pre- and Intensified Postprocedural Physiotherapy in Patients with Symptomatic Aortic Stenosis Undergoing Transcatheter Aortic Valve Replacement Study (the 4P-TAVR Study) by M. Weber, U. Klein, A. Weigert, W. Schiller, V. Bayley-Ezziddin, D. C. Wirtz, A. Welz, N. Werner, E. Grube, G. Nickenig, J.-M. Sinning, A. Ghanem

“…Patients were randomized in a 1 : 1 fashion. 108 patients were included and followed up for 90 days after TAVR. …”
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Acquisition of innate B cell properties and generation of autoreactive IgA antibodies by follicular B cells during homeostatic proliferation by Laura Kampe, Laura Kampe, Christian Melcher, Christian Melcher, Katrin Westphal, Katrin Westphal, Korbinian Brand, Niko Föger, Niko Föger, Kyeong-Hee Lee, Kyeong-Hee Lee

“…Employing adoptive transfer of different mature B cell subsets into lymphopenic Rag2-/- hosts, we here show that not only innate B cells – MZBs and B1a cells – but also adaptive FoBs were capable to differentiate into PCs and to produce IgM and class-switched IgA serum antibodies in a T cell-independent fashion during homeostatic proliferation. In light of the poor reactivity of FoBs to innate stimulation in vitro, the observed high expansion capacity of FoBs, their sustained repopulation of lymphoid and intestinal organs and their particularly prominent ability to induce class-switched auto-/polyreactive IgA antibodies in this antigen- and T cell-independent system was rather unexpected. …”
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Enhanced Interleukin 6 Trans-Signaling Modulates Disease Process in Amyotrophic Lateral Sclerosis Mouse Models by Carol Milligan, Dale O. Cowley, William Stewart, Alyson M. Curry, Elizabeth Forbes, Brian Rector, Annette Hastie, Liang Liu, Gregory A. Hawkins

“…<b>Methods:</b> We leveraged unique mouse models of IL6 trans-signaling that we developed that recapitulate the production of active sIL6R in a genotypic and quantitative fashion observed in humans. Given that the SOD1 transgenic mouse is one of the most highly studied and characterized models of ALS, we bred <i>SOD1^G93A</i> mice with IL6R trans-signaling mice to determine how enhanced trans-signaling influenced symptom onset and pathological processes, including neuromuscular junction (NMJ) denervation, glial activation and motoneuron (MN) survival. …”
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Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide by Audrey Le Bas, Bradley R. Clarke, Tanisha Teelucksingh, Micah Lee, Kamel El Omari, Andrew M. Giltrap, Stephen A. McMahon, Hui Liu, John H. Beale, Vitaliy Mykhaylyk, Ramona Duman, Neil G. Paterson, Philip N. Ward, Peter J. Harrison, Miriam Weckener, Els Pardon, Jan Steyaert, Huanting Liu, Andrew Quigley, Benjamin G. Davis, Armin Wagner, Chris Whitfield, James H. Naismith

“…Both inward- and outward-facing conformations highlight two pairs of arginine residues that move in a reciprocal fashion, enabling flipping. One of the arginine pairs coordinated to a glutamate residue is essential for activity along with the C-terminal arginine rich tail located close to the entrance of the lumen. …”
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The AFFIRM Framework for gender-affirming care: qualitative findings from the Transgender and Gender Diverse Health Equity Study by Meg Quint, Schuyler Bailar, Alexis Miranda, Shalender Bhasin, Devin O’Brien-Coon, Sari L. Reisner

“…Themes about gender-affirming care coalesced into the acronym AFFIRM: (1) Affirms in individual interactions: Participants called for affirmation of TGD identity, lived expertise, and competent TGD providers and staff. (2) Flexible and accessible: Participants expressed the need for gender-affirming care to be available beyond urban population-specific clinics, in a timely fashion without long wait lists, and in a community-centered manner such as offering non-traditional times and settings. (3) Fights systemic oppression: Participants emphasized the need for providers and health systems to eliminate gatekeeping practices for gender-affirming care and create care models that resist intersecting oppressive systems such as racism and cisgenderism. (4) Interacts with community: Patients desired intentional interaction with TGD community to holistically address health and unmet gender affirmation needs. (5) Retains patients in care: Patients shared the need to collaboratively identify and problem-solve obstacles to gender-affirming care with providers and healthcare systems to optimize TGD-specific retention strategies. (6) Multidisciplinary: Patients called for interdisciplinary teams with co-located services such as primary care and mental healthcare with letter-writing for surgical care, and incorporation of peer navigators to meet the broader social, health, and well-being needs of TGD people. …”
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Coagulation Profile of Convalescent Plasma Donors and Recipients by Hanna H Pitkänen MD, PhD, Tuukka Helin MD, PhD, Tamim Khawaja MD, Jukka-Pekka Pietilä MD, Mikael Kajova MD, Hanna Välimaa MD, PhD, DDS, Tero Vahlberg MSc, Jarkko Ihalainen MD, Antti Vierikko MD, Olli Vapalahti MD, PhD, Anu Kantele MD, PhD, Riitta Lassila MD, PhD

“…Fifty-four patients randomized in a double-blinded fashion received either placebo, low-titer CP (LCP) or high-titer CP (HCP). …”
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Random resistive memory-based deep extreme point learning machine for unified visual processing by Shaocong Wang, Yizhao Gao, Yi Li, Woyu Zhang, Yifei Yu, Bo Wang, Ning Lin, Hegan Chen, Yue Zhang, Yang Jiang, Dingchen Wang, Jia Chen, Peng Dai, Hao Jiang, Peng Lin, Xumeng Zhang, Xiaojuan Qi, Xiaoxin Xu, Hayden So, Zhongrui Wang, Dashan Shang, Qi Liu, Kwang-Ting Cheng, Ming Liu

“…The co-design system is validated on 3D segmentation (ShapeNet), event recognition (DVS128 Gesture), and image classification (Fashion-MNIST) tasks, achieving accuracy comparable to conventional systems while delivering 6.78 × /21.04 × /15.79 × energy efficiency improvements and 70.12%/89.46%/85.61% training cost reductions.…”
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Patient-centered outcomes for gastrointestinal cancer care: a scoping review protocol by Natalie G Coburn, Frances C Wright, Alyson L Mahar, Lesley Gotlib Conn, Julie Hallet, Amy T Hsu, Paul D James, Joanna Yang, Tori Barabash, Luckshi Rajendran, Claire Ludwig, Ekaterina Kosyachkova, Julie Deleemans

“…Often, however, these outcomes are not included in research protocols in a standardised and practical fashion. This scoping review will summarise the existing literature on PCOs in gastrointestinal (GI) cancer care as well as the tools used to assess these outcomes. …”
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TGF-β3 Induces Autophagic Activity by Increasing ROS Generation in a NOX4-Dependent Pathway by Yun Zhang, Hong-Mei Tang, Chun-Feng Liu, Xie-Fang Yuan, Xiao-Yun Wang, Ning Ma, Guo-Feng Xu, Song-Ping Wang, Jun Deng, Xing Wang

“…In our previous study, we also observed that TGF-β3 increases mucus secretion in airway epithelial cells in an autophagy-dependent fashion. Although it is well known that the relationship between ROS and autophagy is cell context-dependent, the exact mechanism of action remains unclear. …”
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Lateral Extra-Articular Tenodesis as an Adjunct to ACL Reconstruction by Madeline M. McGovern MD, Sercan Yalcin MD, Natalie A. Lowenstein MPH, Jillian L. Mazzocca BA, Elizabeth G. Matzkin MD, Giovanna Medina MD, PhD

“…Remaining subcutaneous tissue and skin are closed in standard fashion based on the order of the surgery. Results: In the STABILITY randomized controlled trial, 2-year outcomes demonstrated a clinically and statistically significant decrease in clinical failure from 40% to 25% and graft rupture rate from 11% to 4% with the addition of LET to ACLR with hamstring autograft. …”
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Design of an improved model using federated learning and LSTM autoencoders for secure and transparent blockchain network transactions by R. Vijay Anand, G. Magesh, I. Alagiri, Madala Guru Brahmam, Balamurugan Balusamy, Chithirai Pon Selvan, Haya Mesfer Alshahrani, Masresha Getahun, Ben Othman Soufiene

“…Smart contracts are responsible for the submission, validation, and execution of the locally updated models in a decentralized fashion such that the management process is transparent and tamper resistant. …”
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Association of coronary revascularisation after physician-referred non-invasive diagnostic imaging tests with outcomes in patients with suspected coronary artery disease: a post ho... by Takao Kato, Moriaki Inoko, Naoya Matsumoto, Yukari Uemura, Masanao Naya, Mitsuru Momose, Satoshi Hida, Takao Yamauchi, Takatomo Nakajima, Eriko Suzuki, Tohru Shiga, Nagara Tamaki

“…Although in each stratum, the cumulative 1 year incidence of MACEs was significantly higher in patients who underwent revascularisation than in those who did not (SPECT stratum: 9.1 vs 1.2%, log-rank p&lt;0.0001; CT stratum: 6.1 vs 0.8%, log-rank p=0.0001), there was no interaction between the risk of revascularisation and the imaging strata (SPECT stratum: adjusted HR (95% CI), 4.25 (1.86–9.72); CT stratum: 4.13 (1.16–14.73); interaction: p=0.97).Conclusion The association of revascularisation with the outcomes of patients with suspected coronary artery disease was not different between SPECT-first and CT-first strategies in a physician-referred fashion.…”
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Unity in diversity: navigating global connections through cultural exchange by Dwi Mariyono, Annis Nur Alifatul Kamila, Akmal Nur Alif Hidayatullah

“…While globalization can erode local traditions, it also fosters cultural enrichment, seen in fusion cuisine, multilingualism and ethical fashion. The research identifies strategies for balancing global unity and cultural diversity, such as hybrid approaches to environmental sustainability, integrating cultural insights into global health and inclusive technological innovations like AI and virtual reality. …”
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A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens by Ralf Baumann, Susanne Kaempfer, Novel N. Chegou, Wulf Oehlmann, Ralf Spallek, André G. Loxton, Paul D. van Helden, Gillian F. Black, Mahavir Singh, Gerhard Walzl

“…In the present study, IgA and/or IgG responses to the mycobacterial protein antigens AlaDH, NarL, 19 kDa, PstS3, and MPT83 were determined in a blinded fashion in sera from 53 LTBI controls, 14 healthy controls, and 42 active TB subjects. …”
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Effect of glass fiber hybridization and radiation treatment to improve the performance of sustainable natural fiber-based hybrid (jute/glass) composites by S.H. Mahmud, S.C. Das, A. Saha, T. Islam, D. Paul, M.W. Akram, M.S. Jahan, M.Z.I. Molla, M.A. Gafur, R.A. Khan

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No Need to Know: Toward Astrophysics-free Gravitational-wave Cosmology by Amanda M. Farah, Thomas A. Callister, Jose María Ezquiaga, Michael Zevin, Daniel E. Holz

“…Furthermore, it is commonly presumed that the form of the mass distribution must be known a priori to obtain unbiased measurements of cosmological parameters in this fashion. Here, we demonstrate that spectral sirens can accurately infer cosmological parameters without such prior assumptions. …”
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Use of Indocyanine Green (ICG) to Assess Myocardial Perfusion and Territorial Distribution of Vein Grafts Implanted on Coronary Arteries in an Ex-vivo Porcine Model. A Potential Ad... by Cristiano Spadaccio, Antonio Nenna, Diletta Corrado, Carter Glenn, Antonio Panza, Russell Vester, Grzegorz Laskawski, David Rose, Louis Louis

“…In a separate set of experiments, SVG was used for anastomosis in end-to-side fashion to a terminal obtuse marginal (OM) branch. …”
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