Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo by Claudia Palazzo, Roberta Mastrantonio, Noemi Gioelli, Erika Testa, Francesco Recco, Donatella Lucchetti, Giulia Villari, Alessio D’Alessio, Alessandro Sgambato, Flavio Mignone, Guido Serini, Maria Teresa Viscomi, Luca Tamagnone

“…Gene knock-down and overexpression experiments demonstrated that the loading of miR-210-3p into exosomes is dependent on NRP1. …”
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Ruthenium(II) Complex with 1-Hydroxy-9,10-Anthraquinone Inhibits Cell Cycle Progression at G0/G1 and Induces Apoptosis in Melanoma Cells by Júlia S. M. Dias, Guilherme A. Ferreira-Silva, Rommel B. Viana, João H. de Araujo Neto, Javier Ellena, Rodrigo S. Corrêa, Marília I. F. Barbosa, Marisa Ionta, Antônio C. Doriguetto

“…Additionally, DNA-binding experiments yielded constants of 10⁵ M⁻¹ (Kb = 6.93 × 10⁵ for (<b>1</b>) and 1.60 × 10⁵ for (<b>2</b>)) and demonstrate that both complexes can interact with DNA through intercalation, electrostatic attraction, or hydrogen bonding. …”
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A qualitative study exploring the acceptability of the TEXTPAD telehealth and virtual supervised exercise intervention and trial for patients with peripheral arterial disease livin... by Maddey Patterson, MPH, Letitia Sermin-Reed, MSc, James Prentis, MBBS, Arathi Radhakrishnan, MBBS, Eileen Kaner, PhD, Sandip Nandhra, MBBS, Maisie Rowland, BSc, Gabriel Cucato, PhD, Mackenzie Fong, PhD

“…TEXTPAD participants wanted more interaction with other participants to share experiences, advice, and information (theme 1). Participants in both groups perceived a range of benefits to their health and well-being (theme 2) and considered the study procedures to be acceptable and not overly burdensome (theme 3). …”
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Oral pre-exposure prophylaxis implementation in South Africa: a case study of USAID-supported programs by Jerome Wendoh Milimu, Lauren Parmley, Mahlodi Matjeng, Mathata Madibane, Mandisi Mabika, Jacques Livingston, Joseph Lawrence, Orapeleng Motlhaoleng, Hasina Subedar, Rethabile Tsekoa, Zandile Mthembu

“…This case study uses WHO's Building Blocks for Health Systems Strengthening to describe USAID's oral PrEP program in South Africa, reporting experiences and lessons learned in 14 districts across 7 provinces. …”
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Identification of WDR74 and TNFRSF12A as biomarkers for early osteoarthritis using machine learning and immunohistochemistry by Yiwei Chen, Yiwei Chen, Jiali Lin, Detong Shi, Yu Miao, Yu Miao, Feng Xue, Feng Xue, Kexin Liu, Kexin Liu, Xiaotao Wang, Changqing Zhang, Changqing Zhang

“…The expression levels of WDR74 and TNFRSF12A were significantly higher in the IL-1β-induced group in an in vitro qPCR experiment. The IHC validation on human knee joint specimens confirmed the upregulation of WDR74 and TNFRSF12A in OA tissues, corroborating their potential as diagnostic biomarkers.ConclusionsWDR74 and TNFRSF12A as principal biomarkers highlighted their attractiveness as therapeutic targets. …”
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Defective Slc7a7 transport reduces erythropoietin compromising erythropoiesis by Judith Giroud-Gerbetant, Fernando Sotillo, Gonzalo Hernández, Irene Ruano, David Sebastián, Joana Fort, Mayka Sánchez, Günter Weiss, Neus Prats, Antonio Zorzano, Manuel Palacín, Susanna Bodoy

“…We analyzed erythropoiesis in these mice and performed bone marrow transplantation experiments to assess the role of Slc7a7 in erythroblasts and myeloid cells. …”
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<i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth by Manegdebwaoga Arthur Fabrice Kabore, Guanpeng Huang, Changqing Feng, Shuhong Wu, Jiayi Guo, Guofeng Wu, Yiqiong Sun, Samuel Tareke Woldegiorgis, Yufang Ai, Lina Zhang, Wei Liu, Huaqin He

“…However, the <i>in vivo</i> experiments showed that Pi promoted plant growth by increasing root and shoot length, chlorophyll content, and nitrogen uptake, particularly in CO39 during pathogen infection. …”
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Low light reduces saffron corm yield by inhibiting starch synthesis by Weijing Yang, Weijing Yang, Xin Li, Xin Li, Fei Chang, Fei Chang, Xue Qiu, Xulong Huang, Xulong Huang, Zhan Feng, Zhan Feng, Jie Yan, Jie Yan, Qinghua Wu, Qinghua Wu, Feiyan Wen, Feiyan Wen, Jin Pei, Jin Pei, Tao Zhou, Tao Zhou

“…In this study, a two-year field experiment was conducted under natural light (NL) and low light (LL, 50% of NL intensity) conditions. …”
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Mfsd2a suppresses colorectal cancer progression and liver metastasis via the S100A14/STAT3 axis by Linfeng Sun, Xiangdong Li, Yuhao Xiao, Wenjie Yu, Xuyang Chen, Ziyi Wang, Nan Xia, Xuejiao Chen, Minhao Chen, Haoliang Zhu, Jie Li, Jie Wei, Sheng Han, Liyong Pu

“…The biological function of Mfsd2a in CRLM was confirmed by a series of experiments conducted both in vitro and in vivo. Additionally, we investigated downstream molecular pathways using western blot, Co-immunoprecipitation, immunofluorescence, and mass spectrometry techniques. …”
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The contrasted impacts of grasshoppers on soil microbial activities in function of primary production and herbivore diet by Ibanez, Sébastien, Foulquier, Arnaud, Brun, Charles, Colace, Marie-Pascale, Piton, Gabin, Bernard, Lionel, Gallet, Christiane, Clément, Jean-Christophe

“…During two consecutive years, we conducted an experiment controlling the presence of three grasshopper species consuming either grasses, forbs or both in twelve natural and managed alpine grasslands with contrasted productivities. …”
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Carbon cycle and climate feedbacks under CO₂ and non-CO₂ overshoot pathways by I. Melnikova, I. Melnikova, P. Ciais, K. Tanaka, K. Tanaka, H. Shiogama, K. Tachiiri, K. Tachiiri, T. Yokohata, O. Boucher

“…Next, we carry out a series of idealised ramp-up and ramp-down concentration-driven experiments and show that, while the impacts of increasing and decreasing <span class="inline-formula">CO₂</span> and non-<span class="inline-formula">CO₂</span> gases on the surface climate are nearly equivalent (when their radiative forcing magnitudes are set to be the same), regional differences emerge. …”
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Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP by Song Guo, Song Guo, Qihao Pan, Qihao Pan, Baokang Chen, Baokang Chen, Yijuan Huang, Yijuan Huang, Si Li, Si Li, Chenyu Gou, Chenyu Gou, Yu Gao, Yu Gao

“…YAP expression in HTR8/SVneo cells was manipulated to investigate its role in trophoblastic senescence and oxidative damage.ResultsCompared with the control group, the AMA placenta exhibits increased aging biomarkers, which is coupled with an elevation in oxidative damage within placental trophoblast cells and a notable decline in YAP levels. Cellular experiments demonstrated that oxidative damage from H2O2 triggered trophoblast cell senescence and resulted in a reduction of YAP levels. …”
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N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis i... by Deng Lai Li, Rui Zhu, Si Tong Meng, Yin Tao Wang, Si Ying Liu, Zhi Yong Yang, Li Fang Wu

“…This experiment investigated the effects of N-Acetylcysteine (NAC) addition to diets on growth performance, mitochondrial function, antioxidant capacity, immune function and apoptosis in Leuciscus waleckii. …”
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LINC01089 governs the miR-1287-5p/HSPA4 axis to negatively regulate osteogenic differentiation of mesenchymal stem cells by Hao Zou, Fei Hu, Xin Wu, Bin Xu, Guifeng Shang, Dong An, Dehao Qin, Xiaolei Zhang, Aofei Yang

“…The binding of miR-1287-5p with LINC01089 and HSPA4 was verified by performing dual-luciferase reporter and RNA immunoprecipitation (RIP) experiments. Results: LINC01089 expression was reinforced in serum samples of OP patients, but it gradually diminished while hMSCs underwent osteogenic differentiation. …”
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Metabolomics, network pharmacology, and microbiome analyses uncover the mechanisms of the Chinese herbal formula for the improvement of meat quality in spent hens by Zhihua Li, Md. Abul Kalam Azad, Chengwen Meng, Xiangfeng Kong, Jue Gui, Wenchao Lin, Yadong Cui, Wei Lan, Qinghua He

“…Chinese herbs have been widely used as medicine for a long time to prevent diseases and as nutrient supplements to improve the product quality. This experiment explored the effects of adding 1.0% Chinese herbal formula (CHF, including 0.30% Leonurus japonicus Houtt., 0.20% Salvia miltiorrhiza Bge., 0.25% Ligustrum lucidum Ait., and 0.25% Taraxacum mongolicum Hand.…”
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Understanding Dioxygen Activation in the Fe(III)-Promoted Oxidative Dehydrogenation of Amines: A Computational Study by Ricardo D. Páez-López, Miguel Á. Gómez-Soto, Héctor F. Cortés-Hernández, Alejandro Solano-Peralta, Miguel Castro, Peter M. H. Kroneck, Martha E. Sosa-Torres

“…O₂ acts as an oxidant and is converted to H₂O. Experiments with the deuterated ligand L³ reveal a primary C-H kinetic isotope effect, <i>k</i>^CH/<i>k</i>^CD = 2.30, suggesting C-H bond cleavage as the rate-determining step. …”
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Reproductive performance of female largemouth bass (Micropterus salmoides) broodstock was enhanced by dietary small peptides by Jie Wei, Mingwei Tao, Jiaming Huang, Hangxian Zhou, Qiyou Xu

“…To research the effects of dietary small peptides on the reproductive performance of largemouth bass (Micropterus salmoides) broodstock, four isonitrogenous and isolipidic diets were prepared by adding 5 % small peptide (SP) on two fish meal levels (40 and 20 %), which were labeled as HF (40 % FM), HS (40 % FM+5 % SP), LF (20 % FM) and LS (20 % FM+5 % SP), respectively. 160 female largemouth bass broodstock (initial body weight: 452.36 ± 15.54 g) were randomly allocated to four groups with 4 tanks per group of 10 fish each. A 30-day feed experiment was conducted. Results showed that gonadosomatic index (GSI) of largemouth bass was higher in the 5 % SP group (8.51 ± 1.57 %) than that in the 0 % SP group (7.74 ± 1.82 %) (P < 0.05); GSI (6.83 ± 0.95 %) and relative fertility (RF, 90.64 ± 15.68 eggs/g) were significantly lower in the 20 % FM groups (P < 0.05), based on two-way ANOVA analysis. …”
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HESS Opinions: Towards a common vision for the future of hydrological observatories by P. Nasta, G. Blöschl, G. Blöschl, H. R. Bogena, S. Zacharias, R. Baatz, G. De Lannoy, K. H. Jensen, S. Manfreda, L. Pfister, L. Pfister, A. M. Tarquis, I. van Meerveld, M. Voltz, Y. Zeng, W. Kustas, X. Li, H. Vereecken, N. Romano

“…In contrast, a small number of extensively instrumented research sites would enable community-based experiments in an unprecedented manner, thereby facilitating a deeper understanding of complex, non-linear processes modulated by scale-dependent feedback and multiscale spatiotemporal heterogeneity. …”
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Non-formal encounters between Israeli families and African immigrant families foster lifelong learning competence by Michal Ganz Meishar

“…This empirical study allows description, analysis, understanding and explanation of the challenges and actions in organizing and holding non-formal encounters from personal experiences as a first source. Findings – The analysis of the data brought up two main categories that represent the actions and insights from the non-formal encounters for developing lifelong learning competences: create a personal dialogue to strengthen trust and confidence; foster parental involvement. …”
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Optimasi Data Tidak Seimbang pada Interaksi Drug Target dengan Sampling dan Ensemble Support Vector Machine by Nabila Sekar Ramadhanti, Wisnu Ananta Kusuma, Annisa Annisa

“…There are many target protein and drug compound existed in compound-protein interaction databases, which many interactions are not validated yet by experiment. This unknown interaction led drug target interaction to become imbalanced data. …”
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