Regulatory roles of RNA binding proteins in the Hippo pathway by Shuchang Peng, Chenglin Li, Yanwen He, Lei Xue, Xiaowei Guo

“…Overall, this review provides mechanistic insights into the roles of eukaryotic RBP proteins in the regulation of crucial signaling cascades.…”
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Specialized contact sites regulate the fusion of chlamydial inclusion membranes by Christine Linton, Jordan Wesolowski, Anna Lobley, Toshiyuki Yamaji, Kentaro Hanada, Fabienne Paumet

“…This regulatory mechanism contrasts with eukaryotic or viral fusion systems that are either composed of multiple proteins or use a change in pH to initiate membrane fusion. …”
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Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression. by Ankita Chavan, Lena Skrutl, Federico Uliana, Melanie Pfister, Franziska Brändle, Laszlo Tirian, Delora Baptista, Dominik Handler, David Burke, Anna Sintsova, Pedro Beltrao, Julius Brennecke, Madhav Jagannathan

“…Noncoding satellite DNA repeats are abundant at the pericentromeric heterochromatin of eukaryotic chromosomes. During interphase, sequence-specific DNA-binding proteins cluster these repeats from multiple chromosomes into nuclear foci known as chromocenters. …”
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Unidirectional MCM translocation away from ORC drives origin licensing by Agata Butryn, Julia F. Greiwe, Alessandro Costa

“…Abstract The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. …”
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Hsp90 and cochaperones have two genetically distinct roles in regulating eEF2 function. by Melody D Fulton, Danielle J Yama, Ella Dahl, Jill L Johnson

“…Protein homeostasis relies on the accurate translation and folding of newly synthesized proteins. Eukaryotic elongation factor 2 (eEF2) promotes GTP-dependent translocation of the ribosome during translation. eEF2 folding was recently shown to be dependent on Hsp90 as well as the cochaperones Hgh1, Cns1, and Cpr7. …”
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Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration by Koji Iwamoto, Satomi Matsuoka, Masahiro Ueda

“…Abstract Excitable systems of eukaryotic chemotaxis can generate asymmetric signals of Ras-GTP-enriched domains spontaneously to drive random cell migration without guidance cues. …”
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Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells by Valeria V. Ishtuganova, Anton V. Sidorin, Anastasiya S. Makeeva, Marina V. Padkina, Andrey M. Rumyantsev

“…The study of phosphate metabolism and its regulation is important for understanding the evolutionary processes of regulatory systems in eukaryotic cells. The methylotrophic yeast <i>Komagataella phaffii</i> is an efficient producer organism, and it is actively used in biotechnological production. …”
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The systemic evolutionary theory of the origin of cancer (SETOC): an update by Antonio Mazzocca, Giovanni Ferraro, Giovanni Misciagna

“…Abstract The Systemic Evolutionary Theory of the Origin of Cancer (SETOC) is a recently proposed theory founded on two primary principles: the cooperative and endosymbiotic process of cell evolution as described by Lynn Margulis, and the integration of complex systems operating in eukaryotic cells, which is a core concept in systems biology. …”
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The S-Layer Glycome—Adding to the Sugar Coat of Bacteria by Robin Ristl, Kerstin Steiner, Kristof Zarschler, Sonja Zayni, Paul Messner, Christina Schäffer

“…Typically, bacterial S-layer glycans are O-glycosidically linked to serine, threonine, or tyrosine residues, and they rely on a much wider variety of constituents, glycosidic linkage types, and structures than their eukaryotic counterparts. As the S-layer glycome of several bacteria is unravelling, a picture of how S-layer glycoproteins are biosynthesized is evolving. …”
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High-Throughput Screening and Molecular Dynamics Simulation of Natural Products for the Identification of Anticancer Agents against MCM7 Protein by Xin Zhang, Hui Chen, Hui Lin, Ronglan Wen, Fan Yang

“…Minichromosome maintenance complex component 7 (MCM7) belongs to the minichromosome maintenance family that is necessary for the initiation of eukaryotic DNA replication. Overexpression of the MCM7 protein is linked to cellular proliferation and is accountable for critical malignancy in many cancers. …”
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Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural p... by Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol

“…Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. …”
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The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response by Thomas Daniel Kraemer, Orlando Daniel Quintanar Haro, Eugen Domann, Trinad Chakraborty, Svetlin Tchatalbachev

“…When transiently expressed in cultured eukaryotic cells, TcpF caused suppression of TLR2-dependent NF-κB activation suggesting for TcpF a role as a factor in E. faecalis that benefits colonization by modulating the host’s immune responses.…”
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Synthetic genomics in crop breeding: Evidence, opportunities and challenges by Yuhan Zhou, Ziqi Zhou, Qingyao Shu

“…These advancements open promising avenues for the application of synthetic genomics in multicellular eukaryotic organisms, particularly in the realm of crop improvement. …”
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Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas by Kin Pan Chung, Daniel Frieboese, Florent Waltz, Benjamin D. Engel, Ralph Bock

“…Abstract Eukaryotic cells are highly compartmentalized, requiring elaborate transport mechanisms to facilitate the movement of proteins between membrane‐bound compartments. …”
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getphylo: rapid and automatic generation of multi-locus phylogenetic trees by T. J. Booth, S. Shaw, P. Cruz-Morales, T. Weber

“…We also demonstrate the flexibility of getphylo across four case studies including bacterial and eukaryotic genomes, and biosynthetic gene clusters. …”
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Evaluation of controls, quality control assays, and protocol optimisations for PacBio HiFi sequencing on diverse and challenging samples by Iraad F. Bronner, Emma Dawson, Naomi Park, Olaf Piepenburg, Michael A. Quail

“…The Darwin Tree of Life (DToL) project aims to generate high-quality reference genomes for all eukaryotic organisms in Britain and Ireland. At the time of writing, PacBio HiFi reads are generated for all samples using the Sequel IIe systems by the Wellcome Sanger Institute’s Scientific Operations teams, however we expect lessons from this work to apply directly to the Revio system too, as core principles of SMRT sequencing remain the same. …”
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The study of the regulatory region of the Drosophila melanogaster Notch gene by new methods of directed genome editing by O. V. Andreyenkov, E. I. Volkova, N. G. Andreyenkova, S. A. Demakov

“…The Notch gene plays a key role in the development of organs and tissues of neuroectodermic origin, including the nervous system. In eukaryotic organisms, the Notch pathway is involved in cell fate determination. …”
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Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

“…Summary: The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. …”
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Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis by Megumi Matsuzawa, Takumi Nakayama, Masa H. Sato, Tomoko Hirano

“…Abstract The Cysteine‐rich secretory proteins (CRISPS), Antigen 5 (Ag5), and Pathogenesis‐related 1 (PR‐1) protein (CAP) superfamily members are found in multiple eukaryotic organisms, including yeasts, animals, and plants. …”
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Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells by Yuanxiu Zhong, Yiyun Duan, Fenju Lai, Jinhua Zhang, Yimin Dai

“…Bioinformatics analyses indicate that LI0758 is an ortholog of Rce1, a eukaryotic CAAX protein endoprotease, sharing a similar subcellular localization on the endoplasmic reticulum (ER). …”
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