Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein by Maliki Ankavay, Nathalie Da Silva, Angela Pollán, Noémie Oechslin, Katja Dinkelborg, Patrick Behrendt, Darius Moradpour, Jérôme Gouttenoire

“…Full-length HEV genomes harboring larger sequence insertions such as an HA epitope tag, a highly sensitive miniaturized luciferase reporter (HiBiT) or a split GFP at these sites conserved their ability to produce infectious virus, with about a 1-log decrease in viral titers. …”
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Targeting RGMb interactions: Discovery and preclinical characterization of potent anti-RGMb antibodies blocking multiple ligand bindings by Maria Meira, Aurore Frey, Neila Chekkat, Magda Rybczynska, Zaki Sellam, Joon Seok Park, Francesca Smylie Gazzaniga, Alexia Parmentier, Marianne Le Gall, Gordon James Freeman, Dennis Lee Kasper, Arlene Helen Sharpe, Eric Rambeaux, Abdijapar Shamshiev

“…These findings map the 2C11 epitope at the membrane-distal N-terminal region of RGMb, which coincides with both PD-L2- and BMP2–4-binding sites. …”
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CD200R1 immune checkpoint blockade by the first-in-human anti-CD200R1 antibody 23ME-00610: molecular mechanism and engineering of a surrogate antibody by Cristina Melero, S. Jimmy Budiardjo, Anahita Daruwalla, Lance Larrabee, Oleg Ganichkin, Alexander J. Heiler, Jill Fenaux, Ben Chung, Germaine Fuh, Yao-Ming Huang

“…Structural analysis suggests how the surrogate, named 23ME–00611, acquires the ortholog binding ability at the equivalent epitope of 23ME–00610. This engineering approach does not require a priori knowledge of structural and functional mapping of antibody–antigen interaction and thus is generally applicable for therapeutic antibody development when desired ortholog binding is lacking. …”
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Repeated social defeat in male mice induced unique RNA profiles in projection neurons from the amygdala to the hippocampus by Rebecca G. Biltz, Wenyuan Yin, Ethan J. Goodman, Lynde M. Wangler, Amara C. Davis, Braedan T. Oliver, Jonathan P. Godbout, John F. Sheridan

“…This induced expression of a hemagglutinin epitope in neurons that project into the hippocampus. …”
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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders by Nincy Debeuf, Sahine Lameire, Manon Vanheerswynghels, Julie Deckers, Caroline De Wolf, Wendy Toussaint, Rein Verbeke, Kevin Verstaen, Hamida Hammad, Stijn Vanhee, Bart N Lambrecht

“…Here, we exploited the rapid advances in single-cell sequencing and TCR repertoire analysis to select the best clones without hybridoma selection, and generated CORSET8 mice (CORona Spike Epitope specific CD8 T cell), carrying a TCR specific for the Spike protein of SARS-CoV-2. …”
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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…We then explored the influence of the Mn2+-decapeptide-phosphate antioxidant complex MDP, known to shield proteins during irradiation, and showed that treatment with MDP preserves B. burgdorferi’s epitopes at 5 kGy irradiation, which crucially prevents cell proliferation. …”
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A broadly neutralizing antibody against the SARS-CoV-2 Omicron sub-variants BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 by Zhe Chen, Leilei Feng, Lei Wang, Li Zhang, Binyang Zheng, Hua Fu, Fengdi Li, Ligai Liu, Qi Lv, Ran Deng, YanLi Xu, Yongfeng Hu, Jianhua Zheng, Chuan Qin, Linlin Bao, Xiangxi Wang, Qi Jin

“…Structural analysis using cryo-electron microscopy (cryo-EM) revealed that CR9 binds to an epitope formed by RBD residues, providing a molecular understanding of its neutralization mechanism. …”
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A single residue switch mediates the broad neutralization of Rotaviruses by Yang Huang, Feibo Song, Yuanjun Zeng, Hui Sun, Roufang Sheng, Xuechun Wang, Liqin Liu, Guoxing Luo, Yanan Jiang, Yaling Chen, Mengxuan Zhang, Shiyin Zhang, Ying Gu, Hai Yu, Shaowei Li, Tingdong Li, Qingbing Zheng, Shengxiang Ge, Jun Zhang, Ningshao Xia

“…A combination of time-resolved cryo-electron microscopy (cryo-EM) and in situ cryo-electron tomography (cryo-ET) analysis reveals a counterintuitive dynamic process of virus inactivation, in which 7H13 asymmetrically binds to a conserved epitope in the capsid-proximal aspect of VP4, triggers a conformational switch in a critical residue—F418—thereby disrupts the meta-stable conformation of VP4 essential for normal viral infection. …”
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Dynamics of stochastic mutation to immunodominance by Yu Wu, Xiaopeng Zhao, Mingjun Zhang

“…Although a virus contains several epitopes that can be recognized by cytotoxic T lymphocytes (CTL), the immune responses against different epitopes are not uniform. …”
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Are Ancient Durum Wheats Less Toxic to Celiac Patients? A Study of α-Gliadin from Graziella Ra and Kamut by M. Stella Colomba, Armando Gregorini

“…Several aminoacid substitutions or deletions were detected in p31–49, DQ2-Glia-α1 and DQ2-Glia-α2 epitopes, nevertheless, ELISA constantly showed antibody-antigen positive reactions which led us to suggest that mAbs binding was not apparently affected by polymorphisms. …”
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Screening of Diabetes-Associated Autoantigens and Serum Antibody Profiles Using a Phage Display System by Jun Lin, Zhenyu Wang, Hongtao Wang, Yuping Li, Yao Liu, Yige He, Qian Liu, Zichuan Chen, Yuan Ji

“…Conclusion: Human pancreatic and concurrent bacterial-derived protein antigens and their epitopes were identified in this research by the phage display system, which is crucial for distinguishing different types of diabetes.…”
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Potential Application of Tregitopes as Immunomodulating Agents in Multiple Sclerosis by Wassim Elyaman, Samia J. Khoury, David W. Scott, Anne S. De Groot

“…This paper highlights the potential uses of T regulatory cell epitopes (Tregitopes), natural Treg epitopes found to be contained in human immunoglobulins, as immunomodulating agents in MS. …”
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Serological cross-reactivity between Crimean-Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C by Emmanuel A. Maze, George Booth, Georgina Limon, Sandra Belij-Rammerstorfer, Simona R. Tchakarova, Tsviatko Alexandrov, Clare Browning, Ginette Wilsden, Anna B. Ludi, Bryan Charleston, Teresa Lambe, Teresa Lambe

“…Based on an analysis of known epitopes in NP targeted by antibodies in sheep serum, we propose that NP is less cross-reactive because dominant epitopes are highly dissimilar between CCHFV and NSDV.DiscussionGc exhibited a strong cross-reaction while the NP was weakly cross-reactive due to dominant epitopes being highly dissimilar between CCHFV and NSDV. …”
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Leveraging mRNA technology for antigen based immuno-oncology therapies by Wei Zheng, Michele Ceccarelli, Charalampos S Floudas, Siranush Sarkizova

“…Tumor neoantigens are de novo epitopes derived from somatic mutations, avoiding T-cell central tolerance of self-epitopes and inducing immune responses to tumors. …”
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Immunopeptidomics for autoimmunity: unlocking the chamber of immune secrets by Sanya Arshad, Benjamin Cameron, Alok V. Joglekar

“…Abstract T cells mediate pathogenesis of several autoimmune disorders by recognizing self-epitopes presented on Major Histocompatibility Complex (MHC) or Human Leukocyte Antigen (HLA) complex. …”
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Anti-HER2 biparatopic antibody KJ015 has near-native structure, functional balanced high affinity, and synergistic efficacy with anti-PD-1 treatment in vivo by Zheng Wang, Yu Liu, Yunxia Xu, Lin Lu, Zhen Zhu, Baojie Lv, Xin Fang, Yao Tang, Jinhua Wang, Yu Cheng, Ying Hu, Junwen Lou, Peican Wu, Chendan Liu, Yanjun Liu, Xin Zeng, Qing Xu

“…To fully utilize the potential of bpAbs for anti-HER2 drug development, it is crucial to consider formats that offer stability and high-yield production, along with a functional balance between the two epitopes. In this study, we rationally designed a bpAb, KJ015, that shares a common light chain with two Fab arms and exhibits functionally balanced high affinity for two HER2 non-overlapping epitopes. …”
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DDO-adjuvanted influenza A virus nucleoprotein mRNA vaccine induces robust humoral and cellular type 1 immune responses and protects mice from challenge by Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López

“…These results highlight the potential of DDO268 as an effective mRNA vaccine adjuvant and show that an IAV NP mRNA/DDO268 vaccine is a promising approach for generating protective immunity against conserved internal IAV epitopes.IMPORTANCEVaccines that generate neutralizing antibodies and cytotoxic CD8+ T-cells targeting conserved epitopes are ideal for effective protection against viruses. mRNA vaccines combined with the right adjuvant offer a promising solution to this challenge. …”
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Equivariant Hopf Bifurcation in a Time-Delayed Ring of Antigenic Variants by Israel Ncube

“…The host’s immune response is compartmentalised into reactions to major and minor epitopes. We investigate the dynamics of the model, paying particular attention to bifurcation and stability of the uniform nonzero endemic equilibrium. …”
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Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase by Yesenia Osorio-Aguilar, Maria Cristina Gonzalez-Vazquez, Patricia Lozano-Zarain, Ygnacio Martinez-Laguna, Alejandro Carabarin-Lima, Rosa del Carmen Rocha-Gracia

“…In this study, 10 linear B cell epitopes and 13 CTL epitopes and a putative plasminogen-binding motif (252FYNKENGMY260) and the presence of enolase on the surface of different strains of H. influenzae were identified in the enolase sequence of H. influenzae. …”
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The mucosal barrier and anti-viral immune responses can eliminate portions of the viral population during transmission and early viral growth. by Ryan V Moriarty, Athena E Golfinos, Dane D Gellerup, Hannah Schweigert, Jaffna Mathiaparanam, Alexis J Balgeman, Andrea M Weiler, Thomas C Friedrich, Brandon F Keele, Miles P Davenport, Vanessa Venturi, Shelby L O'Connor

“…We also characterized the sequences of T cell epitopes targeted during acute SIV infection, and found that the emergence of escape variants in acutely targeted epitopes can occur on multiple virus templates simultaneously, but that elimination of some of these templates is likely a consequence of additional host factors. …”
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