Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation by Inmaculada Gayoso, Sara Cantisán, Carolina Cerrato, Joaquín Sánchez-García, Carmen Martin, Rafael Solana, Antonio Torres-Gomez, Julian Torre-Cisneros

“…In conclusion, donor age is the only factor significantly associated with the presence of the “differentiated phenotype” in HCMV-specific CD8+ T cells, whereas HCMV replication after transplantation, recipient age, and stem cell source are the factors associated with the production of IFN-γ in response to HCMV epitopes.…”
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Pseudomonas cold shock proteins suppress bacterial effector translocation in Nicotiana benthamiana by Shen Cong, Jun-Zhou Li, Mei-Ran Zhang, Mei-Ran Zhang, Hai-Lei Wei, Wei Zhang

“…Additionally, inoculation with csp15 and csp22 epitopes triggered the pattern-triggered immunity-associated suppression of effector translocations.DiscussionThis study revealed the redundant roles of CSPs in suppressing bacterial effector translocation in vivo, providing deep insights into the PTI elicited by cytoplasmic bacterial proteins.…”
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Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis by Junko Inagaki, Akane Kondo, Luis R. Lopez, Yehuda Shoenfeld, Eiji Matsuura

“…ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. …”
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Structural basis for the interaction between the Drosophila RTK Sevenless (dROS1) and the GPCR BOSS by Jianan Zhang, Yuko Tsutsui, Hengyi Li, Tongqing Li, Yueyue Wang, Salma Laraki, Sofia Alarcon-Frias, Steven E. Stayrook, Daryl E. Klein

“…We further narrowed down the interacting binding epitopes on both dROS1 and BOSS using hydrogen-deuterium exchange mass spectrometry (HDX-MS). …”
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Neurological Disease-Affected Patients, including Multiple Sclerosis, Are Poor Responders to BKPyV, a Human Polyomavirus by Ilaria Bononi, Elisa Mazzoni, Silvia Pietrobon, Maria Rosa Iaquinta, Andrea Caselli, Elena Torreggiani, Maura Pugliatti, Ilaria Casetta, Massimiliano Castellazzi, Enrico Granieri, Fernanda Martini, Mauro Tognon

“…A significant lower prevalence of IgG antibodies against BKPyV VP 1 epitopes, together with a low titer, was detected in sera from MS patients and other inflammatory neurologic diseases than HS. …”
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Molecular Assessment of Domain I of Apical Membrane Antigen I Gene in Plasmodium falciparum: Implications in Plasmodium Invasion, Taxonomy, Vaccine Development, and Drug Discovery by Che Roland Achungu, Damian Nota Anong, Robert Adamu Shey, Cevie Jesenta Tabe

“…These amino acid sequences were utilized to search for antigenic epitopes, therapeutic targets, and conserved short peptides in Domain I of PfAMA1. …”
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Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis by Ali Pouryousef, Bahador Sarkari, Amir Mootabi Alavi, Mostafa Omidian, Fattaneh Mikaeili

“…Results of the current study indicate that the chemical removal of the glycan epitopes of T. cati ES antigens significantly reduces cross-reactivity rates with other parasitic infections. …”
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Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60 by Tina Märker, Jennifer Kriebel, Ulrike Wohlrab, Volker Burkart, Christiane Habich

“…Hsp60 binding involved conserved binding structure(s) and Hsp60 epitopes and was strongest to NZO mouse-derived mature adipocytes. …”
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Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site by Maria Paula Roberti, Pornpimol Charoentong, Yanhong Lyu, Marten Meyer, Stefan B. Eichmüller, Patrick Schmidt, Frank Momburg, Miray Cetin, Felix Hartmann, Nektarios A. Valous, Albrecht Stenzinger, Laura Michel, Peter Lichter, Andreas Schneeweiss, Verena Thewes, Carlo Fremd, Inka Zörnig, Dirk Jäger

“…Based on the somatic mutations, potential MHC binding epitopes were predicted, of which 17 were selected to generate a peptide vaccine. …”
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Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 by Rebeka Nazareth, Pius Horumpende, Tolbert Sonda, Arnold Ndaro, Edson Mollel, Eliakim Paul, Emmanuel Athanase, Jaffu Chilongola

“…A major challenge to malaria vaccine development is identification of protective epitopes and respective protective immune responses. …”
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Identification of HPV16 E1 and E2-specific T cells in the oropharyngeal cancer tumor microenvironment by Christine McInnis, Lakshmi Srinivasan, Anthony J Coyle, Shilpa Bhatia, Glenn J Hanna, Ann Marie Egloff, Brinda Vijaykumar, Qiaomu Tian, Yanbo Sun, Del Leistritz-Edwards, Charles T Quinn, Ravi Uppaluri, Daniel C Pregibon

“…However, specific virally-encoded epitopes and their corresponding immune responses are not fully defined.Methods To understand the OPSCC immune landscape, we conducted a comprehensive single-cell analysis of HPV16+ and HPV33+ primary tumors and metastatic lymph nodes. …”
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Preparation of recombinant myoglobin and investigation of the liquid antigen stability for quality control materials by Yu-Hui Wang, Xi-Feng Sun, Chun-Xin Xu, Feng-Qiang Sun, Rong-Rong Wang, Xiao-Kun Bian, Zhan-Zhao Wang, Qiang Wu

“…The concentrations per gradient measured using different kits had no significant difference (p-value>0.05), which indicated that the reactivity between the Mb antigen and Mb antibodies with different epitopes was good. The effects of different storage buffer, storage temperature and storage times on the stability of liquid Mb were investigated by detecting the concentration changes. …”
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Exploring molecular determinants and pharmacokinetic properties of IgG1-scFv bispecific antibodies by Kristina M.J. Aertker, Minu Ravindra Pilvankar, Tobias M. Prass, Michaela Blech, Fabian Higel, Srinath Kasturirangan

“…Bispecific antibodies (BsAbs) capable of recognizing two distinct epitopes or antigens offer promising therapeutic options for various diseases by targeting multiple pathways. …”
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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies by A. N. Chikaev, A. P. Rudometov, Yu. A. Merkulyeva, L. I. Karpenko

“…Combinatorial biology methods offer a good solution for targeting interactions of specific molecules by a high-throughput screening and are widely used for drug development, diagnostics, identification of novel monoclonal antibodies, search for linear peptide mimetics of discontinuous epitopes for the development of immunogens or vaccine components. …”
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New insights into the N-glycomes of Dictyostelium species by Alba Hykollari, Daniel Malzl, Chunsheng Jin, Carina Eschenbach, Kristína Kianičková, Iain B.H. Wilson, Katharina Paschinger

“…In previous studies on Dictyostelium discoideum, it was demonstrated that their N-glycans, as in most eukaryotes, derive from the Glc3Man9GlcNAc2-PP-Dol precursor; however, unique glyco-epitopes, including intersecting GlcNAc, core α1,3-fucosylation, sulphation and methylphosphorylation, were detected. …”
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Biparatopic binding of ISB 1442 to CD38 in trans enables increased cell antibody density and increased avidity by Jeremy Loyau, Thierry Monney, Marco Montefiori, Fedir Bokhovchuk, Jeremy Streuli, Matthew Blackburn, Arnaud Goepfert, Lydia N. Caro, Samitabh Chakraborti, Stefania De Angelis, Camille Grandclément, Stanislas Blein, M. Lamine Mbow, Ankita Srivastava, Mario Perro, Stefano Sammicheli, Eugene A. Zhukovsky, Michael Dyson, Cyrille Dreyfus

“…It consists of two adjacent anti-CD38 arms targeting non-overlapping epitopes that preferentially drive binding to tumor cells and a low-affinity anti-CD47 arm to enable avidity-induced blocking of proximal CD47 receptors. …”
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A broad spectrum Shigella vaccine based on VirG53 – 353 multiepitope region produced in a cell-free system by Girmay Desalegn, Charlotte Abrahamson, K. Ross Turbyfill, Lucy Pill-Pepe, Leslie Bautista, Chitradevi S. Tamilselvi, Dylan Dunn, Neeraj Kapoor, Barbara Sullinger, Matheo Herrera, Edwin V. Oaks, Jeff Fairman, Marcela F. Pasetti

“…Seeking to refine the vaccine antigenic target and achieve scalable manufacturing compatible with Good Manufacturing Practices, we mapped linear B-cell epitopes spanning the entire VirG protein sequence by probing the immune reactivity of 10-mer peptides (overlapping 4–8 aa) with sera from Shigella-infected rhesus monkeys. …”
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Detection of Dengue Virus-Specific IgM and IgG Antibodies through Peptide Sequences of Envelope and NS1 Proteins for Serological Identification by Pradeep Kumar Nagar, Deepali Savargaonkar, Anupkumar R. Anvikar

“…In this study, linear epitopes of envelope (E) and NS1 proteins were identified to diagnose the DENV. …”
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Construction of prokaryotic system for expression of porcine circovirus type 2 ORF-2 gene fragment by A. G. Galeeva, A. R. Akhunova, K. V. Usoltsev, N. I. Khammadov, M. A. Efimova

“…A genetic insert constructed from the most immunogenic type-specific PCV-2 epitopes based on genotype 2a, 2b, 2d strain and isolate consensus sequence was cloned into the expression vector pET-22b(+) that was incorporated into the Escherichia coli strain Rosetta 2(DE3). …”
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Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt by Ahmed A. Ali, Rasha A. M. Azouz, Nahla A. Hussein, Reem El-Shenawy, Naiera M. Helmy, Yasmine S. El-Abd, Ashraf A. Tabll

“…The ability of Virus-Like Particles (VLPs) to mimic the native virus and incorporate neutralizing and conformational epitopes, while effectively engaging both humoral and cellular immune responses, makes them a promising approach to addressing the challenges in HCV vaccine development. …”
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