Acute and prolonged toxicity assessment of Cannabis sativa extract in rodents and lagomorphs by Alana C. Costa, Arquimedes Gasparotto, Jr., Alana A.K. Garcia, Cícero A.C. Pereira, Emerson L.B. Lourenço, Helena P.G. Joaquim

“…Prolonged administration of 6 months in rats and 9 months in rabbits did not lead to significant changes in organ histopathology, body weight, or behavior. Although liver enzymes were elevated at the highest doses, other biochemical and hematological parameters remained unchanged. …”
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High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis by Arvind Pradip, Daniella Steel, Susanna Jacobsson, Gustav Holmgren, Magnus Ingelman-Sundberg, Peter Sartipy, Petter Björquist, Inger Johansson, Josefina Edsbagge

“…Recent advances in differentiating human induced pluripotent stem cells (hiPSCs) into pure cultures of hepatocyte-like cells expressing functional drug metabolizing enzymes open up possibilities for novel, more relevant human cell based toxicity models. …”
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Here and there: the double-side transgene localization by P. A. Salnikov, A. A. Khabarova, G. S. Koksharova, R. V. Mungalov, P. S. Belokopytova, I. E. Pristyazhnuk, A. R. Nurislamov, P. Somatich, M. M. Gridina, V. S. Fishman

“…The developed assay requires using intentionally designed vectors that lack recognition sites of one or a set of restriction enzymes used for DNA fragmentation. By looping and sequencing these DNA fragments, we obtain special data that allows us to link the two flanking regions of the transposon. …”
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Hindguts of Kyphosus sydneyanus harbor phylogenetically and genomically distinct Alistipes capable of degrading algal polysaccharides and diazotrophy by Cesar T. Facimoto, Kendall D. Clements, W. Lindsey White, Kim M. Handley

“…However, Alistipes are also prevalent in the hindgut of the marine herbivorous fish Kyphosus sydneyanus (Silver Drummer), and analysis of their carbohydrate-active enzyme (CAZyme) encoding gene repertoires suggests Alistipes degrade macroalgal biomass to support fish nutrition. …”
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Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia by Carolina Muscoli, Concetta Dagostino, Sara Ilari, Filomena Lauro, Micaela Gliozzi, Erlisa Bardhi, Ernesto Palma, Vincenzo Mollace, Daniela Salvemini

“…All of these events have been linked to neurotoxicity. The receptors and enzymes involved in the handling of glutamate pathway—specifically NMDARs, glutamate transporter, and glutamine synthase (GS)—have key tyrosine residues which are targets of the nitration process causing subsequent function modification. …”
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Physiological Properties of Dietary Ellagitannin-Rich Preparations Obtained from Strawberry Pomace Using Different Extraction Methods by Juśkiewicz Jerzy, Król Bogusław, Kosmala Monika, Milala Joanna, Zduńczyk Zenon, Żary-Sikorska Ewa

“…To measure animals response, parameters describing the caecal fermentation (ammonia and short-chain fatty acid concentrations, bacterial enzymes activity), blood serum lipoprotein profile, and TBARS content in selected tissues (heart, liver, kidney) were assessed. …”
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Metronidazole-Induced Pancreatitis: Is There Underrecognition? A Case Report and Systematic Review of the Literature by Ibrahim Youssef, Naba Saeed, Mohammad El Abdallah, Kara Huevelhorst, Kais Zakharia

“…Her symptoms improved rapidly, and pancreatic enzymes normalized within 2 days. Of note, she had had an episode of acute pancreatitis 3 years ago, also following metronidazole use, with resolution at discontinuation of the drug. …”
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Animal Models of Steatosis (NAFLD) and Steatohepatitis (NASH) Exhibit Hepatic Lobe-Specific Gelatinases Activity and Oxidative Stress by Giuseppina Palladini, Laura G. Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Stefano Perlini, Andrea Ferrigno, Mariapia Vairetti

“…Gelatinase (MMP-2; MMP-9) activity and protein levels, tissue inhibitors of metalloproteinase (TIMPs), reactive oxygen species (ROS), and thiobarbituric acid-reactive substances (TBARS) were evaluated in the left (LL), median (ML), and right liver (RL) lobes. Serum hepatic enzymes and TNF-alpha were assessed. An increase in gelatinase activity in the NASH model occurred in RL compared with ML. …”
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Blue light irradiation combined with low-temperature storage further enhances postharvest quality of strawberries through improving antioxidant defense and cell wall metabolic acti... by Wei Lu, Wanqing Li, Keke Zhao, Xiaofeng Bai, Yuchang Zhang, Qingyun Li, Zhanjun Xue, Xin-Xin Wang

“…Meanwhile, BL irradiation also mitigated the activity of cell wall-degrading enzymes, thereby reducing the degradation rates of protopectin (PP), cellulose (CEL), and hemicellulose (HCEL), while maintaining the structural integrity of cell walls. …”
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Therapeutic effects of Pueraria lobata (Willd.) Ohwi root and Hovenia dulcis Thunb. extracts on alcoholic liver disease: Network pharmacology and experimental validation by Zhendong Chen, Yu Yue, Hongyan An, Haisu Yan, Hyeok-Joo Park, Pei Lin

“…Biochemical assays and Western blot analyses were performed to assess the levels of oxidative stress markers, antioxidant enzymes, and inflammatory cytokines. In addition, activation of the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway was examined to elucidate the mechanisms underlying the effects of the extract. …”
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Computer simulation of the spatial structure of MUC 1 peptides capable of inhibiting apoptosis by N. V. Ivanisenko, I. N. Lavrik, V. A. Ivanisenko

“…In these complexes, activation of key enzymes in apoptosis, caspases, takes place. One of those macromolecular complexes is DISC (death- inducing signaling complex) playing a central role in the induction of the extrinsic apoptosis pathway. …”
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Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) by Majid Khanzadeh, Seyed Hossein Hoseinifar, Ashkan Zargari, Hien Van Doan

“…Results: The results showed that adding 2.5 % combined algal supplement (CAS) to the diet of Asian seabass increased the activity of digestive enzymes such as lipase, trypsin, and α-amylase in the gastrointestinal tract. …”
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Coordinated conformational changes in P450 decarboxylases enable hydrocarbons production from renewable feedstocks by Wesley Cardoso Generoso, Alana Helen Santana Alvarenga, Isabelle Taira Simões, Renan Yuji Miyamoto, Ricardo Rodrigues de Melo, Ederson Paulo Xavier Guilherme, Fernanda Mandelli, Clelton Aparecido Santos, Rafaela Prata, Camila Ramos dos Santos, Felippe Mariano Colombari, Mariana Abrahão Bueno Morais, Rodrigo Pimentel Fernandes, Gabriela Felix Persinoti, Mario Tyago Murakami, Leticia Maria Zanphorlin

“…Abstract Fatty acid peroxygenases have emerged as promising biocatalysts for hydrocarbon biosynthesis due to their ability to perform C-C scission, producing olefins - key building blocks for sustainable materials and fuels. These enzymes operate through non-canonical and complex mechanisms that yield a bifurcated chemoselectivity between hydroxylation and decarboxylation. …”
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A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways by Duo Wang, Gangli Ma, Jia Shen, Xinyang Xu, Weisong Shou, Zhengying Xuan, Yanjun He

“…Furthermore, the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activity. The qRT-PCR results further indicated that the expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathways were upregulated in the transgenic tobacco lines. …”
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Bioethanol production from coconut husk using DES-NADES pretreatment and enzymatic hydrolysis method by Yerizam, Muhammad, Jannah, Asyeni Miftahul, Aprianti, Nabila, Yandriani, Yandriani, Rendana, Muhammad, Ernas, Annisa Qonita, Tamba, Juice Lowise

“…The delignified samples were hydrolyzed using the 5% (w/w) of cellulase enzymes and fermented for seven days using Saccharomyces cerevisiae. …”
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Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products by T. N. Shapiro, N. A. Manucharova, E. S. Lobakova

“…Alkanmonooxygenase enzymes AlkB and Cyp153 are responsible for the aerobic degradation of n-alkanes of petroleum and petroleum products. …”
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The clinical value of serum sirtuin-1 concentration in the diagnosis of metabolic dysfunction-associated steatotic liver disease by Farshad Amirkhizi, Mahdiyeh Taghizadeh, Banafshe Khalese-Ranjbar, Soudabeh Hamedi-Shahraki, Somayyeh Asghari

“…Anthropometric parameters, demographic information, and serum biochemical variables—including glycemic parameters, lipid profiles, liver enzymes, and Sirt-1 levels—were assessed. The correlation between serum Sirt-1 and biochemical variables was examined using Pearson’s correlation coefficient. …”
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Effect of melatonin on the contents of fatty acids and antioxidants of saffron by Mohammad Mehdi Samim, Ali Sorooshzadeh, Ali Mokhtassi-Bidgoli, Mohammad Sadegh Sabet

“…Also, the highest activities of ascorbate peroxidase and catalase enzymes were observed with 100 μM melatonin applied 124 days after germination. …”
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Synthesis, Characterization, and Antiproliferative Effect of CuO-TiO2-Chitosan-Amygdalin Nanocomposites in Human Leukemic MOLT4 Cells by Abozer Y. Elderdery, Badr Alzahrani, Siddiqa M. A. Hamza, Gomaa Mostafa-Hedeab, Pooi Ling Mok, Suresh Kumar Subbiah

“…To demonstrate the nanocomposite’s anticancer effects, MTT assay, morphological analysis, apoptosis studies using acridine orange/ethidium bromide (AO/EtBr) dual staining, reactive oxygen species (ROS) analysis, and levels of antioxidant enzymes were analyzed using the MOLT4 blood cancer cell line. …”
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TET2-mediated 5-hydroxymethylcytosine of TXNIP promotes cell cycle arrest in systemic anaplastic large cell lymphoma by Ziqing Yu, Lihua Zhong, Wangyang Tang, Wenfang Zhang, Ting Lin, Weifeng Zhu, Gang Chen, Jianchao Wang

“…GEO dataset was used to analyze the expression 5hmC-relative enzymes. Immunohistochemistry was conducted to assess the level of 5hmC and Ten-eleven translocation 2 (TET2) on FFPE samples. …”
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