Poly (ADP-ribose) (PAR) levels in Peripheral Blood Mononuclear Cells (PBMC) during peri-implantation and early pregnancy in buffalo cows by Giovanna De Matteis, Anna Reale, Michele Zampieri, Emanuela Rossi, Federica Signorelli, Olimpia Barbato, Vittoria Lucia Barile

“… Poly(ADP-ribosyl)ation (PAR) is a reversible post-translational protein modification catalyzed by enzyme  members of the poly(ADP-ribose) polymerases (PARPs) family. …”
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The role of Anti-PAD4, Anti-CarP, and Anti-RA33 antibodies combined with RF and ACPA in predicting abatacept response in rheumatoid arthritis by Alberto Floris, Maria Maddalena Angioni, Mattia Fadda, Micaela Rita Naitza, Mattia Congia, Elisabetta Chessa, Matteo Piga, Alberto Cauli

“…Positivity for aPAD4, aCarP and aRA33 were determined by Enzyme-Linked Immunosorbent Assay (ELISA). The achievement of a moderate or good EULAR response at 6 months and the 3-years retention were analyzed as treatment outcomes. …”
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Identifying health risk determinants and molecular targets in patients with idiopathic pulmonary fibrosis via combined differential and weighted gene co-expression analysis by Abu Tayab Moin, Md. Asad Ullah, Jannatul Ferdous Nipa, Mohammad Sheikh Farider Rahman, Afsana Emran, Md. Minhazul Islam, Swapnil Das, Tawsif Al Arian, Mohammad Mahfuz Enam Elahi, Mukta Akter, Umme Sadea Rahman, Arnab Halder, Shoaib Saikat, Mohammad Jakir Hosen

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Efek Pemberian Vitamin C terhadap Gambaran Histopatologi Hati Tikus Wistar yang Terpapar Timbal Asetat by Arifuddin ., Aswiyanti Asri, Elmatris .

“…</p><p><strong>Kata kunci:</strong> Timbal Asetat, Gambaran Histopatologi Hati, Vitamin C, Plumbum Asetat, Manja Roenigk</p><p> </p><p><strong><em>Abstract</em></strong></p><p><em>Exposure of lead acetate can caused cell injury in liver and decrease activity of enzyme which works as essential antioxidant in body. …”
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Evaluation of dietary coffee Coffee canephora husk supplementation on the growth, blood chemicals, and antioxidative activity of red Nile tilapia Oreochromis sp. by Azmi Afriansyah, Mia Setiawati, Muhammad Agus Suprayudi, Ichsan Achmad Fauzi

“…Superoxide dismutase enzyme was also found higher in coffee husk supplemented diets than in control diet (P<0.05). …”
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29 m6A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation – 102 Transcriptomic Dataset... by Ming Liu, Keman Xu, Fatma Saaoud, Ying Shao, Ruijing Zhang, Yifan Lu, Yu Sun, Charles Drummer, Li Li, Sheng Wu, Satya P. Kunapuli, Gerard J. Criner, Jianxin Sun, Huimin Shan, Xiaohua Jiang, Hong Wang, Xiaofeng Yang

“…We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA methylation (epitranscriptomic) regulators (m6A-RMRs) in 41 diseases and cancers and made significant findings: (1) a few m6A-RMRs were upregulated; and most m6A-RMRs were downregulated in sepsis, acute respiratory distress syndrome, shock, and trauma; (2) half of 29 m6A-RMRs were downregulated in atherosclerosis; (3) inflammatory bowel disease and rheumatoid arthritis modulated m6A-RMRs more than lupus and psoriasis; (4) some organ failures shared eight upregulated m6A-RMRs; end-stage renal failure (ESRF) downregulated 85% of m6A-RMRs; (5) Middle-East respiratory syndrome coronavirus infections modulated m6A-RMRs the most among viral infections; (6) proinflammatory oxPAPC modulated m6A-RMRs more than DAMP stimulation including LPS and oxLDL; (7) upregulated m6A-RMRs were more than downregulated m6A-RMRs in cancer types; five types of cancers upregulated ≥10 m6A-RMRs; (8) proinflammatory M1 macrophages upregulated seven m6A-RMRs; (9) 86% of m6A-RMRs were differentially expressed in the six clusters of CD4+Foxp3+ immunosuppressive Treg, and 8 out of 12 Treg signatures regulated m6A-RMRs; (10) immune checkpoint receptors TIM3, TIGIT, PD-L2, and CTLA4 modulated m6A-RMRs, and inhibition of CD40 upregulated m6A-RMRs; (11) cytokines and interferons modulated m6A-RMRs; (12) NF-κB and JAK/STAT pathways upregulated more than downregulated m6A-RMRs whereas TP53, PTEN, and APC did the opposite; (13) methionine-homocysteine-methyl cycle enzyme Mthfd1 downregulated more than upregulated m6A-RMRs; (14) m6A writer RBM15 and one m6A eraser FTO, H3K4 methyltransferase MLL1, and DNA methyltransferase, DNMT1, regulated m6A-RMRs; and (15) 40 out of 165 ROS regulators were modulated by m6A eraser FTO and two m6A writers METTL3 and WTAP. …”
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Dietary citral increase growth and health performance of shrimp Penaeus vannamei by Ricky Hadi Pratama, Julie Ekasari, Muhammad Agus Suprayudi, Ichsan Achmad Fauzi, Wiyoto Wiyoto

“…The level of amylase enzyme, post-prandial blood glucose, muscle, and liver glycogen were higher in the treatments with dietary citral than those of the controls. …”
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Selection of potential probiotic candidate bacteria from seawater and shrimp pond sediments for controlling the pathogenic Vibrio parahaemolyticus by Anisa Zulfani, Munti Yuhana, Sukenda Sukenda, Usamah Afif

“…The results of the study successfully obtained 37 probiotic candidate isolates with 10 isolates having the greatest enzyme activity. LAZ-2 (Acinetobacter radioresistens) isolated from seawater and SAZ-22 (Fusobacterium varium) isolated from pond sediment have been selected as probiotic candidate bacteria that have beneficial enzymatic activities. …”
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Effects of Microbial Fertilizer Application on Soil Ecology in Saline–Alkali Fields by Xingguo Tian, Xiu Zhang, Guoping Yang, Yu Wang, Qianru Liu, Jingjing Song

“…It provides a scientific basis for the remediation of the saline–alkali field via microbe-induced changes in soil physicochemical properties, enzyme activity, microbial diversity, and community structure at different periods.…”
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Contents of plasma М2-pyruvate kinase (M2-РК) in patients with inflammatory bowel diseases by Ye. A. Konovich, I. L. Khalif

“…Blood plasma concentration of M2-РК was determined by solid-phase sandwich method of enzyme-linked immunoassay with application of «ScheBo» (Germany) test-systems.Results. …”
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Development and Evaluation of an Immunochromatographic Strip and a Magnetic Chemiluminescence Immunoassay for Detection of <i>Porcine Circovirus Type 2</i> Antigen by Sirui Tao, Yu Duan, Yinhe Zha, Xiaxia Tong, Yulong He, Huapeng Feng, Jianhong Shu

“…To date, immunoperoxidase monolayer assay (IPMA), indirect immunofluorescent assay (IFA), and enzyme linked immunosorbent assay (ELISA) are the most commonly diagnostic methods for detecting PCV2 antigens. …”
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Emodin protects against severe acute pancreatitis-associated acute lung injury by activating Nrf2/HO-1/GPX4 signal and inhibiting ferroptosis in vivo and in vitro by Gang Shen, Haiyun Wen, Huijuan Li, Xuetao Zhang, Bowen Lan, Xuanchi Dong, Peng Ge, Yalan Luo, Hailong Chen

“…Pancreatic and lung tissue damage was evaluated using histological examination and molecular experiments. Enzyme-linked immunosorbent assays (ELISA) were used to assess the levels of pro-inflammatory cytokines, Fe2+, and associated oxidative stress indicators in the serum and cell supernatant. …”
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Antiemetic activity of Sesamol possibly through serotonergic and dopaminergic receptor interaction pathways: In vivo and in silico studies by Touhidul Islam Tanim, Ayman M. Al-Qaaneh, Raihan Chowdhury, Md. Shimul Bhuia, Tabassum Islam, Md Showkoth Akbor, Md. Tahajul Islam, Md. Mohasin Miah, Ali Raza Ishaq, Mostafa A. Abdel-Maksoud, Mohamed A. El-Tayeb, Mohamed El-Shazly, Muhammad Torequl Islam, Heba A.S. El-Nashar

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Development and optimization of a new competitive ELISA using recombinant (rPSA D15 and rCag11) antigens for the detection of Helicobacter pylori infection. by Biniam Moges Eskeziyaw, Naomi Maina, Rebecca Waihenya, Matthew Mutinda Munyao, Tonny Teya Nyandwaro, Shingo Inoue, Samson Muuo Nzou

“…Therefore, this study aimed to develop and validate a competitive enzyme-linked immunosorbent assay (cELISA) assay for detecting H. pylori infections by targeting the Protective Surface Antigen (PSA) and Cytotoxic-Associated Gene Pathogenesis Island (Cag11) proteins in H. pylori stool antigen sample. …”
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Effectiveness of Electronic Quality Improvement Activities to Reduce Cardiovascular Disease Risk in People With Chronic Kidney Disease in General Practice: Cluster Randomized Trial... by Jo-Anne Manski-Nankervis, Barbara Hunter, Natalie Lumsden, Adrian Laughlin, Rita McMorrow, Douglas Boyle, Patty Chondros, Shilpanjali Jesudason, Jan Radford, Megan Prictor, Jon Emery, Paul Amores, An Tran-Duy, Craig Nelson

“…One module within FHT aims to facilitate cardiovascular disease (CVD) risk reduction in people with chronic kidney disease (CKD) through the recommendation of angiotensin-converting enzyme inhibitor inhibitors (ACEI), angiotensin receptor blockers (ARB), or statins according to Australian guidelines (defined as appropriate pharmacological therapy). …”
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Neuroprotective Actions of Cannabinoids in the Bovine Isolated Retina: Role of Hydrogen Sulfide by Leah Bush, Anthonia Okolie, Jenaye Robinson, Fatima Muili, Catherine A. Opere, Sunny E. Ohia, Ya Fatou Njie Mbye

“…In some experiments, retinas were pretreated with inhibitors of the biosynthesis of H₂S [cystathionine β-synthase/cystathionine γ-lyase (CBS/CSE), aminooxyacetic acid, AOAA 30 µM, or 3-mercaptopyruvate sulfurtransferase (3MST), α-keto-butyric acid, KBA 1 mM] and the CB1-receptor antagonist, AM251 (100 nM) for 30 min before treatment with methanandamide (1 nM–100 µM). Enzyme immunoassay measurement of 8-epi PGF2α (8-isoprostane) levels was performed to assess lipid peroxidation in retinal tissues. …”
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Identification of the Granule-Bound Starch Synthase (GBSS) Genes Involved in Amylose Biosynthesis in Tartary Buckwheat (<i>Fagopyrum tataricum</i> (L.) Gaertn.) by Juan Huang, Fei Liu, Jieqiong Zhang, Bin Tang, Jiao Deng, Taoxiong Shi, Liwei Zhu, Hongyou Li, Qingfu Chen

“…The granule-bound starch synthase (GBSS) is a key enzyme responsible for the synthesis of amylose, directly determining the amylose content and amylose-to-amylopectin ratio in crops. …”
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Impact of donor CYP3A5 genotype on pharmacokinetics of tacrolimus in South African paediatric liver transplant patients by C Wheeler, C Masimirembwa, B Mthembu, J Botha, J Scholefield, J Fabian

“…The rs776746 polymorphism, CYP3A5*3, results in a splice defect and a non-functional enzyme. Clinically, to reach the same tacrolimus concentration-to-dose ratio (CDR), expressors (CYP3A5*1/*1 and *1/*3) require a higher tacrolimus dose than non-expressors (*3/*3). …”
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Renin–angiotensin–aldosterone system activation in plasma as marker for prognosis in critically ill patients with COVID-19: a prospective exploratory study by Katharina Krenn, Felix Kraft, Luana Mandroiu, Verena Tretter, Roman Reindl-Schwaighofer, Theresa Clement, Oliver Domenig, Matthias G. Vossen, Gregor Riemann, Marko Poglitsch, Roman Ullrich

“…Angiotensin-converting enzyme (ACE) and ACE2 concentrations were measured by LC–MS/MS-based assays. …”
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Patterns of beta-blocker use and dose optimization among ambulatory heart failure patients with reduced ejection fraction (HFrEF) attending public hospitals in Northeast Ethiopia:... by Minimize Hassen, Tsehaynesh Kebede Ebsa, Oumer Sada Muhammed, Tewodros Solomon, Mulat Belete Demessie, Teklehaimanot Fentie Wendie, Nuhamin Alemayehu Tesfaye, Mengistie Yirsaw Gobezie

“…Factors positively associated with beta-blocker use included Angiotensin Converting Enzyme Inhibitor use (AOR: 15.48, 95% CI: 2.11-113.54, p = 0.007), and taking multiple medications (AOR: 7.12, 95% CI: 1.54–33.02, p = 0.012), while ingestion of secondary prevention agents (AOR: 0.05, 95% CI: 0.01–0.98, p = 0.048) and male gender (AOR: 0.08, 95% CI: 0.01–0.47, p = 0.005) were negatively associated. …”
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