Identification and Analysis of Melon (<i>Cucumis melo</i> L.) SHMT Gene Family Members and Their Functional Studies on Tolerance to Low-Temperature Stress by Yanmin Liu, Dandan He, Yizhou Wu, Kangqi Zhao, Changyi Yang, Yulu Zhong, Liuyang Yang, Haiyue Niu, Sushuang Liu

“…Serine hydroxymethyltransferase (SHMT), a conserved phosphopyridoxal-dependent enzyme, plays a crucial role in plant resistance to abiotic stressors. …”
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Telomerase inhibiting phytochemicals derived from Blumea eriantha for cancer treatment: A comprehensive computational analysis by Rahil Ummar Faruk Abbu, Shaik Mohammad Abdul Fayaz, Divyashree M S, Raghu Chandrashekhar H, Prasanna Kumar Reddy Gayam, Archana Mahadev Rao

“…Background: One potential treatment target for cancer cells is telomerase, an essential enzyme involved in the longevity of cancer cells. …”
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Association between ABCB4 variants and intrahepatic cholestasis of pregnancy by Dekun Zhang, Yuhong Li, Shufeng He, Jing Shu, Tiechen Li, Qing Sun

“…Abstract The ABCB4 gene encodes multidrug resistance protein 3(MDR3), which is a phosphatidylcholine(PC) transfer enzyme that transfers lecithin from the inner part of the phospholipid bilayer to the extracellular bile. …”
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Effects of supplementation with vitamin D3 on growth performance, lipid metabolism and cecal microbiota in broiler chickens by Jiawei Li, Ximei Li, Jiamin Tian, Linna Xu, Yan Chen, Susu Jiang, Guohua Zhang, Jianxiong Lu

“…VD3 increased mRNA expressions of genes related to de novo lipogenesis (DNL) (mTOR, SREBP-1c, FAS and ACC), lipid oxidation (AMPK, PPARα, CPT-1α and ACO) and lipid transport (ApoB and MTTP), and FAS, ACC and CPT1 enzyme activities in the liver. However, mRNA levels of genes involved in DNL and cellular lipid uptake (LPL and FATP1) and LPL activity were decreased in abdominal adipose tissue, and that of genes involved in lipid oxidation and lipolysis (HSL and ATGL) was increased by VD3. …”
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Neutrophil extracellular traps (NETs) are increased in rheumatoid arthritis-associated interstitial lung disease by Jing Xue, Miaomiao Nian, Yangyang Liang, Zeqin Zhu, Zhenyu Hu, Yuanyuan Jia, Shuhong Chi, Juan Chen

“…The clinical relevance of plasma myeloperoxidase-DNA (MPO-DNA), citrullinated histone 3 (Cit-H3), and cell-free DNA was evaluated in RA-ILD patients with different imaging types via a commercial enzyme-linked immunosorbent assay (ELISA). Results In the ZYM-treated SKG mouse model, which recapitulates key features of RA-ILD, an increased population of neutrophils in the lung tissue was primarily responsible for NET formation. …”
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Plant-Based Antigen Production Strategy for SARS-CoV-2 Nucleoprotein and RBD and Its Application for Detection of Antibody Responses in COVID-19 Patients by Katerina Takova, Valeria Tonova, Ivan Minkov, Eugenia S. Mardanova, Nikolai V. Ravin, Stanislav Kotsev, Maria Pishmisheva, Gergana Zahmanova

“…The purified N protein and HEV/RBD protein were used to develop an indirect enzyme-linked immunosorbent assay (iELISA) for the detection of antibodies to SARS-CoV-2 in human sera. …”
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The Predictive Value of Growth Differentiation Factor-15 in Recurrence of Atrial Fibrillation after Catheter Ablation by Ying Wei, Shuwang Liu, Haiyi Yu, Yuan Zhang, Wei Gao, Ming Cui, Lei Li

“…Blood samples on the second morning of hospital admission and three months after RFCA were collected, and enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of GDF-15. …”
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Association of Light’s Criteria with Pleural Fluid Procalcitonin Levels and Ultrasound Thorax with its Impact on the Management of Pleural Effusion: A Cross-sectional Study by BK Likhitha, Keertivardhan D Kulkarni, Madhav Mahawar

“…Ultrasound of the thorax was performed on each patient, Light’s criteria were calculated and pleural fluid PCT levels was measured via Enzyme-linked Immunusorbent Assay (ELISA). Categorical variables between the two groups (exudative group and transudative group) were compared using the Chi-square/Mann-Whitney U test, with p<0.05 considered statistically significant. …”
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TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p by Huikang Xu, Jiamin Fu, Lijun Chen, Sining Zhou, Yangxin Fang, Qi Zhang, Xin Chen, Li Yuan, Yifei Li, Zhenyu Xu, Charlie Xiang

“…The small EVs secreted by TNF-α-stimulating MenSCs were more effective in colonic mice than those secreted directly by MenSCs, as evidenced by the results of histopathology analysis of colonic tissue, immunohistochemistry for tight junction proteins, and enzyme-linked immunosorbent assay (ELISA) for cytokine expression profiles in vivo. …”
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Investigation into the influence of mild hypothermia on regulating ferroptosis through the P53-SLC7A11/GPX4 signaling pathway in sepsis-induced acute lung injury by Liujun Tao, Jie Xu, Liangyan Jiang, Juntao Hu, Zhanhong Tang

“…This reduction in cystine availability compromises the activity of Glutathione peroxidase 4 (GPX4), a cystine-dependent enzyme, ultimately increasing cellular susceptibility to ferroptosis. …”
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Serum Level Alteration of IL-6, IL-1β, and IFN-γ in Groups of Healthy Adults with Oxidative DNA Damage in Najaf Governorate by Dhuha S. Saleh, Hayder S. Hussain, Hasan N. Al-Haidari, Samia K. Abbas, Ayaid K. Zgair, Seenaa M. Ali

“…The present study aims to determine the effect of uranium pollution and DNA damage on the immune system function in terms of estimating the levels of serum interleukin (IL)-6, interferon-gamma (IFN-γ), and IL-1 beta (β). Method. Enzyme-linked immunosorbent assay (ELISA) (Sandwich method technique) was used for estimating the serum cytokines levels in test and control groups. …”
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Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…TNF-α protein levels in cell lysates were measured by enzyme-linked immunosorbent assay (ELISA). The DOR agonist TAN-67 and antagonist naltrindole (NTI) were used to pretreat cells for 1 or 2 hours before OGD24/R36 administration. …”
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High-Grade Cervical Intraepithelial Neoplasia: Impact of Colposcopic Lesion Area on Systemic Immune Responses by Mantoani PTS, Micheli DC, Jammal MP, Vieira JH, Michelin MA, Ferreira CGX, Silva HN, Murta EFC, Nomelini RS

“…The objectives of the study were to determine whether colposcopic lesion area is associated with serum levels of cytokines IL (interleukin) − 1, IL-6, IL-8, IL-10, IL-12 and TNF-α in precursor lesions of cervical cancer.Methods: The study population comprised patients with high-grade squamous intraepithelial lesion who had undergone colposcopy, cervical biopsy, and measurements of serum cytokines by ELISA (Enzyme-Linked Immunosorbent Assay). Genotyping for HPV (human papillomavirus) 16, 18, 45 and 52 was performed by PCR (Polymerase Chain Reaction). …”
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Acute effect of endurance exercise on human milk insulin concentrations: a randomised cross-over study by Rebecca Lyng Holm, Mads Holmen, Md Abu Jafar Sujan, Md Abu Jafar Sujan, Guro F. Giskeødegård, Trine Moholdt, Trine Moholdt

“…We determined insulin concentrations in the milk using enzyme-linked immunosorbent assay and compared insulin concentrations after MICT and HIIT with REST using a linear mixed model with time-points and the interaction between time and condition as fixed factors.ResultsWe detected insulin in all 240 samples, with an average concentration of 12.3 (SD 8.8) μIU/mL (range 3.2–57.2 μIU/mL). …”
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Remote Continuous Microinjury-Triggered Cytokines Facilitate Severe Diabetic Foot Ulcer Healing via the Ras/Raf/MEK/ERK Pathway by Huang X, Liu J, Wu X, Mo Y, Luo X, Yang Y, Yang C, Liang X, Liang R, Chen Y, Fan Z, Lu W, Chen Y, Hua Q

“…Wound measurement and histology were performed to evaluate the wound healing processes. Enzyme-linked immunosorbent assay, flow cytometry, immunohistochemistry, and Western Blot were used to assess angiogenesis and the activity of endothelial progenitor cells (EPCs) and the Ras/Raf/MEK/ERK signaling pathway.Results: We found accelerated wound healing, improved epidermal continuity, and increased dermal thickness in the TTT group than the control and the sham groups. …”
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Association of serum and local GRP78 and CHOP expressions with disease progression in patients with non-traumatic osteonecrosis of femoral head by Peng Zhang, Qing-He Ye, Wen-Xiu Zhu, Yong-Heng Zhao, Hong-Xun Zhu, Biao-Fang Wei

“…Serum CHOP and GRP78 levels were determined through enzyme linked immunosorbent assay (ELISA). Local CHOP and GRP78 expressions were detected by immunohistochemistry, western blot, alongside real-time polymerase chain reaction (RT-PCR). …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer&rsquo;s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…C57BL/6J mice and Hippocampal neuronal cell line (HT-22) were used to prepare the AD model. Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. …”
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Genome polymorphism of the synthetic species xTrititrigia cziczinii Tsvel. inferred from AFLP analysis by A. A. Trifonova, K. V. Boris, L. V. Dedova, V. A. Melnik, L. P. Ivanova, N. P. Kuzmina, S. V. Zavgorodniy, V. P. Upelniek

“…For the study, AFLP method (Amplified Fragment Length Polymorphism) was chosen, which allowed us to reveal a sufficiently high polymorphism level of the studied samples. The two primer/enzyme combinations (EcoRI-ACT/MseI-CCC, EcoRI-ACT/MseI-CTA) allow ed the iden tification of 227 fragments, 224 of them were polymorphic (98.68 %), and the level of intraspecific polymorphism of 24 ×T. cziczinii samples was 68.15 %. …”
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Circulating Total and Active Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinases-1 in Patients with Systemic Lupus Erythomatosus

“…Serum levels of tMMP-9 and TIMP-1 were assessed by an enzyme-linked immunosorbent assay (ELISA) and aMMP-9 by fluorometric assay. …”
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Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…The decrease in intestinal villus height, villous height: crypt depth ratio and digestibility was accompanied by lower expression of ZO1, ZO2, Claudin1, DMT1, CAT1, SGLT1 and PBD114 genes, as well as decreased enzyme activities of intestinal alkaline phosphatase, lactase, sucrase and maltase of piglets in DA group compared to piglets in DCON group. …”
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