Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency by Yunting Zhou, Junming Zhou, Yumin Zhang, Jun Tang, Bo Sun, Wei Xu, Xiaohang Wang, Yang Chen, Zilin Sun

“…The intestinal morphology, intestinal permeability, and inflammatory response activation signaling pathway were assessed using histological staining, western blots, quantitative-PCR, and enzyme-linked immunosorbent assays. Results. VAD diet-fed mice displayed reduction of tissue VA levels, increased area under the curve (AUC) of glucose challenge, reduced glucose-stimulated insulin secretion, and loss of β cell mass. …”
Get full text

Growth Arrest Specific Gene 6 Protein Concentration in Cerebrospinal Fluid Correlates with Relapse Severity in Multiple Sclerosis by P. P. Sainaghi, L. Collimedaglia, F. Alciato, R. Molinari, D. Sola, E. Ranza, P. Naldi, F. Monaco, M. Leone, M. Pirisi, G. C. Avanzi

“…Gas6 was measured (CSF, plasma) by in-house-enzyme-linked immunoassay (ELISA). Results. Higher CSF Gas6 concentrations were observed in relapses lasting ≤60 days (8.7 ± 3.9 ng/mL) versus >60 days (6.5 ± 2.6) or controls (6.5 ± 2.4; P=0.05), with ≤2 FS involved (8.5 ± 3.8) versus >2 FS (5.6 ± 2.5) (P<0.05) and EDSS change ≤2.5 points (8.8 ± 3.7) versus >2.5 (6.5 ± 3.5) (P=0.04). …”
Get full text

Oxidative Stress and Risk of Dementia in Older Patients with Depression: A Longitudinal Cohort Study Using Plasma Biomarkers by Yoo-Jin Jang, Min-Ji Kim, Su-Jin Lee, Shinn-Won Lim, Doh-Kwan Kim

“…Biomarkers, such as nitrotyrosine, protein carbonyl, F2-isoprostanes, malondialdehyde, 4-hydroxynonenal, and 8-hydroxy-2′-deoxyguanosine, were collected at baseline and measured using an enzyme-linked immunosorbent assay. AD conversion was determined using comprehensive neuropsychological assessment. …”
Get full text

Photosynthetic performance and carbon metabolism in the ear organs of oats under drought stress by Jiaqi Fang, Yuan Zhan, Baowen Zhao, Yan Zhao, Youjun Chen, Qingping Zhou, Hui Wang

“…Paraffin cross-section structures were observed, and the absolute water content, photosynthetic enzyme activities, carbohydrate content, dry matter weight, and total C and total N accumulation were determined in the glumes, lemmas and flag leaves. …”
Get full text

Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals by Dina Aboelsoued, Nagwa I. Toaleb, Kadria N. Abdel Megeed

“…Therefore, this study aimed to evaluate an early diagnosis of cryptosporidiosis using the anti-Cryptosporidium parvum oocyst immunoglobulin IgG polyclonal antibodies (anti-C. parvum IgG PAbs)-based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium oocyst antigens in fecal samples of farm animals in Egypt. …”
Get full text

Matrix Metalloproteinase-2 Isoforms Differ within the Aortic Wall of Ascending Aortic Aneurysms Associated with Bicuspid Aortic Valve by Ramona Schmitt, Anke Tscheuschler, Philipp Laschinski, Philipp Discher, Jana Fuchs, Fabian A. Kari

“…We therefore analyzed aTAA tissue levels of MMP-2 (matrix metalloproteinase-2) isoforms (Pro-MMP-2, active MMP-2, and total MMP-2) and tissue levels of MMP-14, TIMP-2 (tissue inhibitor of metalloproteinase-2), MMP-9, and TIMP-1 in 19 patients with BAV and 23 patients with TAV via gelatin zymography and enzyme-linked immunosorbent assay (ELISA), respectively. …”
Get full text

Liver damage in patients living with HIV on antiretroviral treatment with normal baseline liver function and without HBV/HCV infection: an 11-year retrospective cohort study in Gua... by Jun Yu, Hao Liang, Fengxiang Qin, Junjun Jiang, Chunwei Qin, Yunxuan Huang, Bingyu Liang, Yuexiang Xu, Jiegang Huang, Zhiliang Xu, Chuanyi Ning, Yanyan Liao, Ning Zang, Jingzhen Lai, Wudi Wei, Li Ye, Xionglin Qin

“…The association between duration of ART and liver damage (grade II–IV liver enzyme elevation [LEE] and/or total bilirubin elevation [TBE]), was analysed. …”
Get full text

Experimental Validation of Antiobesogenic and Osteoprotective Efficacy of Ginsenoside CK via Targeting Lipid and Atherosclerosis Pathways by Md. Niaj Morshed, Reshmi Akter, Imran Mahmud, Ah-Yeong Gwon, Jin Woo Jeang, Yeong-Geun Lee, Dae Won Park, Deok Chun Yang, Yeon Ju Kim, Se-Chan Kang

“…Using 3T3-L1 adipocytes in vitro assessments, CK could effectively decrease intracellular lipid accumulation, inhibit α-glucosidase enzyme, increase 2-NBDG glucose uptake, reduce inflammation-associated cytokines (<i>TNFα</i>, and <i>IL-6</i>), adipogenic regulatory genes (<i>PPARγ</i>, <i>FAS</i>, <i>C/EBPα</i>), lipogenic gene <i>LPL</i>, and increase the expression of thermogenic gene <i>UCP1</i>. …”
Get full text

Unveiling the Potency of Gardenia Extract Against <i>H. pylori</i>: Insights from In Vitro and In Vivo Studies by Pornpen Werawatganone, Duangporn Werawatganon, Nattida Noonak, Maneerat Chayanupatkul, Tanittha Chatsuwan, Naruemon Klaikeaw, Walaisiri Muangsiri, Prasong Siriviriyakul

“…The serum and stomach tissue of the rats were evaluated using enzyme-linked immunosorbent assay, histopathology, and immunohistochemistry. …”
Get full text

Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The aims of this work were i) to determine whether it is possible to create recombinant K. phaffii K51 clones overexpressing functionally active proteinase K from T. album and ii) to analyze the enzymatic activity of the resulting recombinant enzyme. The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
Get full text

Inhibition Mechanism of Pleurotus citrinipileatus Sing. Ergothioneine on Pancreatic Lipase Activity by Zhangchen XIA, Xiaohui MENG, Ru FANG, Yanqing MAO, Junwen CHENG, Yanbin WANG, Juan XU, Liang HE

“…The interaction between Pleurotus citrinipileatus Sing. ergothioneine (PCEGT) and pancreatic lipase (PL) was studied by analyzing multiple spectroscopies including enzyme kinetics, ultraviolet absorption (UV), fluorescence spectroscopy, synchronous fluorescence spectroscopy and 3D fluorescence spectroscopy as well as the PCEGR-PL interaction mechanism by molecular docking in order to clarify its inhibitory mechanism. …”
Get full text

Identification of novel pQTL‐SNPs associated with lung adenocarcinoma risk: A multi‐stage study by Yutong Wu, Huiwen Xu, Liping Mao, Rongrong Zhao, Junfeng Chu, Lili Huang, Wendi Zhang, Yiran Liu, Qiong Chen, Xiaobo Tao, Siqi Li, Shenxuan Zhou, Anhui Ning, Zhenyu Li, Tian Tian, Lei Zhang, Jiahua Cui, Guangyu Tian, Minjie Chu

“…The associations between selected pQTL‐SNPs and LUAD risk were evaluated by Female Lung Cancer Consortium in Asia GWAS (FLCCA GWAS). Enzyme linked immunosorbent assay (ELISA) is used to determine the levels of candidate protein. …”
Get full text

A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice by Peng Xu, Shulei Hao, Xiaoxia Wen, Guifang Ma, Qinqin Yang, Ling Liu, Galal Bakr Anis, Yingxin Zhang, Lianping Sun, Xihong Shen, Qunen Liu, Daibo Chen, Yongbo Hong, Yuyu Chen, Xiaodeng Zhan, Shihua Cheng, Liyong Cao, Weixun Wu

“…<i>Ehd2</i> was revealed as a modulator of salt tolerance, likely through the regulation of ion transport, enzyme activity, and antioxidant systems. This study establishes <i>Ehd2</i> as a pivotal factor in promoting heading while negatively regulating salt tolerance in rice.…”
Get full text

Rosiglitazone, a Ligand to PPARγ, Improves Blood Pressure and Vascular Function through Renin-Angiotensin System Regulation by María Sánchez-Aguilar, Luz Ibarra-Lara, Leonardo Del Valle-Mondragón, María Esther Rubio-Ruiz, Alicia G. Aguilar-Navarro, Absalom Zamorano-Carrillo, Margarita del Carmen Ramírez-Ortega, Gustavo Pastelín-Hernández, Alicia Sánchez-Mendoza

“…In this work, we hypothesized that RGZ exerts a PPARγ–dependent regulation of blood pressure through modulation of angiotensin-converting enzyme (ACE)-type 2 (ACE2)/angiotensin-(1-7)/angiotensin II type-2 receptor (AT2R) axis in an experimental model of high blood pressure. …”
Get full text

Targeting enolase 1 reverses bortezomib resistance in multiple myeloma through YWHAZ/Parkin axis by Xuejie Gao, Qilin Feng, Qikai Zhang, Yifei Zhang, Chaolu Hu, Li Zhang, Hui Zhang, Guanli Wang, Ke Hu, Mengmeng Ma, Zhuning Wang, Yujie Liu, Dong An, Hongfei Yi, Yu Peng, Xiaosong Wu, Gege Chen, Xinyan Jia, Haiyan Cai, Jumei Shi

“…Abstract Background Enolase 1 (ENO1) is a conserved glycolytic enzyme that regulates glycolysis metabolism. However, its role beyond glycolysis in the pathophysiology of multiple myeloma (MM) remains largely elusive. …”
Get full text

High-speed centrifugation reduces immune rejection by removing bone marrow elements from fresh osteochondral allografts by Yongsheng Ma, Wenming Yang, Qitai Lin, Meiming Li, Zehao Li, Yugang Xing, Lei Wei, Wangping Duan, Xiaochun Wei

“…Moreover, the co-culture effect of RAW264.7 cells and OCAs with or without HSC in vitro was evaluated using enzyme-linked immunosorbent assay (ELISA), histological staining, and immunohistochemical staining. …”
Get full text

Prognostic Value of CXCL13, CCL11, and CCL20 Chemokines in Multiple Sclerosis by Işıl Peker, Hacer Eroğlu İçli, Belgin Mutluay, Burcu Yüksel, Zeynep Özdemir, Mesrure Köseoğlu, Aysu Şen, Dilek Ataklı, Aysun Soysal, Musa Öztürk

“…Chemokine levels were measured using enzyme-linked immunosorbent assay (ELISA) in serum and cerebrospinal fluid (CSF) samples. …”
Get full text

Protocol for a randomised ‘screen-and-treat’ Helicobacter pylori eradication trial in 14–18-years-old adolescents residing in three regions of Chile: effectiveness and microbiologi... by Ximena Aguilera, Camila Cabrera, Sergio George, Yalda Lucero, Beatriz Zabala Torres, Lilian Fernández, Nora Mamani, Anne Lagomarcino, Miguel O'Ryan

“…Concurrently, a subset of 60 uninfected adolescents will undergo matched follow-up. Enzyme-linked immunosorbent assay (ELISA) commercial kits will evaluate gastric damage biomarkers in serum (pepsinogen I and II, gastrin-17, VCAM-1, CXCL13). …”
Get full text

Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…The inflammatory factor levels were performed by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). Alcian blue staining and immunofluorescence were used to estimate the intestinal barrier. …”
Get full text

<i>Rubus idaeus RiACS1</i> Gene Is Involved in Ethylene Synthesis and Accelerates Fruit Ripening in <i>Solanum lycopersicum</i> by Tiemei Li, Wenjiao Xin, Hang Zhang, Jiarong Jiang, Kunmiao Ding, Mengyu Liu, Nanyan Li, Guohui Yang

“…Raspberry is a berry whose fruit is not tolerant to storage; breeding varieties with extended storage time and high comprehensive quality are significant for raspberries in cold regions. 1-Aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) is a limiting enzyme in the ethylene synthesis process, which plays essential roles in fruit ripening and softening in plants. …”
Get full text