SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma ce... by Tianci Han, Xiaoqi Guo, Junwei Xie, Wei Tong, Liang Zhang

“…The E2 small ubiquitin-like modifier (SUMO) binding enzyme UBC9 increased ETV1 activity and expression, indicating the presence of SUMO modification in ETV1. …”
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Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold by Dwikora Novembri Utomo, Ferdiansyah Mahyudin, Teddy Heri Wardhana, Purwati Purwati, Febrian Brahmana, Agrippina Waya Rahmaning Gusti

“…Biochemical characteristics were obtained by enzyme-linked immunosorbent assay for type II collagen, aggrecan, and Transforming Growth Factors-β (TGF-β). …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Then the cell viability, inflammatory factors, and the expression of epithelial–mesenchymal transition (EMT) and fibrosis markers were determined by using Cell Counting Kit-8 (CCK-8) assay, enzyme linked immunosorbent assay (ELISA), western blotting assays, respectively. …”
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Altered CD73-Adenosine Signaling Linked to Infection in Patients undergoing hemodialysis by Xiang F, Zhang Z, Nie Y, Cao X, Li Y, Chen X, Gong S, Zou J, Teng J, Ding X, Shen B

“…Fangfang Xiang,1– 4,* Zhen Zhang,1,* Yuxin Nie,1 Xuesen Cao,1 Yang Li,1 Xiaohong Chen,1 Shaomin Gong,1 Jianzhou Zou,1 Jie Teng,1 Xiaoqiang Ding,1– 4 Bo Shen1– 4 1Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China; 2Shanghai Key Laboratory of Renal Disease and Blood Purification, Shanghai, People’s Republic of China; 3Shanghai Medical Center of Kidney, Shanghai, People’s Republic of China; 4Shanghai Institute of Kidney and Dialysis, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bo Shen, Department of Nephrology, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, People’s Republic of China, Email shen.bo@zs-hosptial.sh.cnPurpose: Infection is the most common cause of hospitalization and the second most common cause of mortality among patients undergoing hemodialysis (HD). The enzyme CD73, a cell surface 5′-nucleotidase, regulates the balance between pro-inflammatory nucleotides and anti-inflammatory adenosine. …”
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Synthesis, Antibacterial, Antioxidant, and Molecular Modeling Studies of Novel [2,3′-Biquinoline]-4-Carboxylic Acid and Quinoline-3-Carbaldehyde Analogs by Zeleke Digafie, Yadessa Melaku, Zerihun Belay, Rajalakshmanan Eswaramoorthy

“…The molecular docking analysis of the compounds against E. coli DNA gyrase was computed to investigate the binding mode of the compounds within the active site of the enzyme. In this regard, a new series of [2,3′-biquinoline]-4-carboxylic acid and quinoline-3-carbaldehyde analogs were synthesized by utilization of Vilsmeier–Haack, Doebner, nucleophilic substitution, and hydrolysis reactions. …”
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Human cytomegalovirus (HCMV) trends in Sri Lanka: insights from a hospital-based seroprevalence analysis by Narapity Pathirannehalage Sunil-Chandra, Madawala Vithanage Madusha Lakmali Jayasundara, Maththe Gama Ralalage Shobha Sanjeewani Gunathilaka, Suduwa Dewage Himal Suminda

“…Methods A total of 820 blood samples obtained from 820 participants of selected low and high-risk populations in Sri Lanka, namely; hospital inpatients (n = 200), antenatal women (n = 80), blood donors (n = 80), cancer patients (n = 200), Sexually Transmitted Disease (STD) clinic attendees (n = 160) and Chronic Kidney Disease (CKD) patients on haemodialysis (n = 100) were tested for IgG antibodies against HCMV by Enzyme Linked Immunosorbent Assay (ELISA). Statistical analysis was performed using IBM SPSS Statistics version 30 (IBM® Corporation, New York, USA). …”
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The MSDIN family in amanitin-producing mushrooms and evolution of the prolyl oligopeptidase genes by Hong Luo, Qing Cai, Yunjiao Lüli, Xuan Li, Rohita Sinha, Heather E. Hallen-Adams, Zhu L. Yang

“…Prolyl oligopeptidase B (POPB), a key enzyme in the biosynthetic pathway, was used in phylogenetic reconstruction to infer the evolutionary history of the genes. …”
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Enhanced Activity by NKCC1 and Slc26a6 Mediates Acidic pH and Cl− Movement after Cardioplegia-Induced Arrest of db/db Diabetic Heart by Minjeong Ji, Seok In Lee, Sang Ah Lee, Kuk Hui Son, Jeong Hee Hong

“…Interestingly, expression of Slc26a6, as well as carbonic anhydrase IV as a supportive enzyme of Slc26a6, was increased in db/db cardiac strips compared to wild type cardiac strips. …”
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Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway by Dengjun Guo, Jie Zhou, Meng Zhang, Reyisha Taximaimaiti, Xiaoping Wang, Hai Wang

“…High-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA) were used to determine neurotransmitters and metabolites, inflammatory factors TNF-α and IL-1β, oxidative stress-related markers SOD, MDA, and GSH in striatum tissues. …”
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CD2AP deficiency aggravates Alzheimer’s disease phenotypes and pathology through p38 MAPK activation by Yan-Yan Xue, Zhe-Sheng Zhang, Rong-Rong Lin, Hui-Fen Huang, Ke-Qing Zhu, Dian-Fu Chen, Zhi-Ying Wu, Qing-Qing Tao

“…Then, we generated and characterized an APP/PS1 mouse model with neuron-specific Cd2ap deletion, using immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay, electrophysiology and behavioral tests. …”
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The Effect of A2E on the Ca2+-PKC Signaling Pathway in Human RPE Cells Exposed to Blue Light by Maomei Luo, Shu Wang, Yun Tang, Chun Zeng, Shanjun Cai

“…The intracellular levels of inositol triphosphate (IP3) and diacylglycerol (DAG) were measured by enzyme-linked immunosorbent assay (ELISA). Intracellular PKC activity was measured with a nonradioactive nuclide assay. …”
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TAp63γ is the primary isoform of TP63 for tumor suppression but not development by Xinbin Chen, Wenqiang Sun, Xiangmudong Kong, Xin Ming, Yanhong Zhang, Wensheng Yan, Shakur Mohibi, Mingyi Chen, Keith Mitchell, Jin Zhang

“…Single-cell RNA-seq revealed that loss of p63γ increases the expression of SCD1, a rate-limiting enzyme for synthesis of monounsaturated fatty acids, leading to altered lipid homeostasis. …”
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The serum level of sclerostin decreases in radiographic axial spondyloarthritis patients with fatty lesions by Xuegang Li, Haijian Jiang, Xu Wang, Shuping Zhong

“…Serum levels of bone turnover markers, including sclerostin, osteoprotegerin (OPG), procollagen I N-terminal propeptide (PINP), cross linked C-telopeptide of type I collagen (CTX-I), osteocalcin (OC), were measured using enzyme-linked immunosorbent assays. Results There were no significant differences between two groups in terms of gender, age, body mass index (BMI), duration, smoking, HLA-B27 positivity rate, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), BASDAI, ASDAS-ESR, ASDAS-CRP, biological disease-modifying anti-rheumatic drugs (bDMARDs) rate. …”
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Effects of Different Microplastics on Methane Production and Microbial Community Structure in Anaerobic Digestion of Cattle Manure by Mengjiao Zhang, Congxu Zhao, Tian Yuan, Qing Wang, Qiuxian Zhang, Shuangdui Yan, Xiaohong Guo, Yanzhuan Cao, Hongyan Cheng

“…Based on changes observed in key enzyme functional gene abundances, PHA MPs reduced acetyl-CoA carboxylase functional gene abundance, negatively affecting the acetone cleavage methanogenesis pathway. …”
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Exercise training partly ameliorates cardiac dysfunction in mice during doxorubicin treatment of breast cancer by Tytti-Maria Uurasmaa, Pauline Bourdin, Wail Nammas, Shiva Latifi, Heidi Liljenbäck, Antti Saraste, Olli Eskola, Johan Rajander, Anne Roivainen, Helene Rundqvist, Anu Autio, Ilkka Heinonen, Katja Anttila

“…The cardiac histology, oxidative stress, maximal metabolic enzyme activities, and mitochondrial respiration were analyzed. …”
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Development and Validation of a Predictive Model Based on Serum Silent Information Regulator 6 Levels in Chinese Older Adult Patients: Cross-Sectional Descriptive Study by Yuzi You, Wei Liang, Yajie Zhao

“…Serum specimens of eligible patients were collected for SIRT6 detection by enzyme-linked immunosorbent assay. Clinical data and putative predictors, including 29 physiological characteristics, biochemical parameters, carotid artery ultrasonographic results, and complete coronary angiography findings, were evaluated, with CAD diagnosis as the primary outcome. …”
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Assessment of Plasma Sodium to Potassium Ratio, Renal Function, Markers of Oxidative Stress, Inflammation, and Endothelial Dysfunction in Nigerian Hypertensive Patients by Oloruntoba A. Ekun, Folasade Daniel, Philip Adebola, Adeola Ajibare, Oyeronke O. Ekun, Oluwafunso O Omogoroye, Oluwafemi S. Ilori, Bankole J. Oluwasayo, Mary F. Oshundun, Sade R. Oyegbami

“…Blood samples were collected from the participants and were analyzed for electrolytes, markers of oxidative stress, endothelial dysfunction, renal function, and inflammation, using ion-selective electrodes, spectrophotometric, and enzyme-linked immunosorbent assay methods, respectively. …”
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On the Biosynthesis of Bioactive Tryptamines in Black Cohosh (<i>Actaea racemosa</i> L.) by Martin J. Spiering, James F. Parsons, Edward Eisenstein

“…Co-expression in yeast of the high-activity black cohosh TDCs with the next enzyme in serotonin biosynthesis, tryptamine 5-hydroxylase (T5H), from rice (<i>Oryza sativa</i>) resulted in the production of serotonin, whereas co-expression with low-activity TDCs did not, suggesting that TDC activity is a rate-limiting step in serotonin biosynthesis. …”
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Evaluating the Sustainability of Wheat–Maize System with a Long-Term Fertilization Experiment by Yun Shao, Jiahui An, Xueping Wang, Shouchen Ma, Ye Meng, Yang Gao, Shoutian Ma

“…The results indicated that following prolonged fertilization, the soil nutrient content, enzyme activity, and crop yield of each organic fertilizer treatment were significantly greater than those of the chemical fertilizer treatment alone, resulting in a more stable yield. …”
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Exogenous organic acids promoted phytoremediation by Hydrangea macrophylla in cadmium‑contaminated soil by Yunjing Song, Qian Cheng, Bing Zhao

“…Additionally, organic acids increased the soil pH, accompanied by changes in soil enzyme activities. 10 mmol·kg−1AA and MA reduced the available Cd concentration by 20.42 % and 31.65 %, respectively. …”
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