Seroprevalence of Peste des Petits Ruminants and Contagious Caprine Pleuropneumonia Coinfections in Goats in Kwale County, Kenya by George Lugonzo, George Gitao, Lilly Bebora, Harrison Osundwa Lutta

“…Herein, the competitive enzyme-linked immunosorbent assay (cELISA) and the latex agglutination test (LAT) were used to determine the coinfections of PPR and CCPP in goats in Kwale County on Kenya’s South Coast. …”
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Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Domestic Ruminants in East Hararghe Zone of Oromia Region, Ethiopia by Berhanu Tilahun, Yacob Hailu Tolossa, Getachew Tilahun, Hagos Ashenafi, Shihun Shimelis

“…Sera of 1360 domestic ruminants were analyzed for the presence of anti-T. gondii IgG antibodies using the indirect enzyme-linked immunosorbent assay (iELISA). Additionally, the owners were also interviewed using a structured questionnaire to identify the potential risk factors of T. gondii infection. …”
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Effect of Locally Delivered Melatonin as an Adjunct to Nonsurgical Therapy on GCF Antioxidant Capacity and MMP-9 in Stage II Periodontitis Patients: A Randomized Controlled Clinica... by Enji Ahmed, Olfat G. Shaker, Nermin Yussif, Dalia M. Ghalwash

“…Total antioxidative capacity (TAC) and matrix metalloproteinase-9 (MMP-9) levels in GCF were also evaluated utilizing commercially available enzyme-linked immunosorbent assay kits (ELISA) at baseline and 3 months after therapy. …”
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Do Salivary <i>Cullin7</i> Gene Expression and Protein Levels Provide Advantages over Plasma Levels in Diagnosing Breast Cancer? by Ceren Tilgen Yasasever, Derya Duranyıldız, Süleyman Bademler, Hilal Oğuz Soydinç

“…Protein levels of blood and saliva samples taken from breast cancer patients and a healthy control group were measured by the ELISA (Enzyme-Linked Immunosorbent Assay) method. Gene expression levels between the two groups were analyzed by the qPCR (quantitative Polymerase Chain Reaction) method. …”
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Large Gliadin Peptides Detected in the Pancreas of NOD and Healthy Mice following Oral Administration by Susanne W. Bruun, Knud Josefsen, Julia T. Tanassi, Aleš Marek, Martin H. F. Pedersen, Ulrik Sidenius, Martin Haupt-Jorgensen, Julie C. Antvorskov, Jesper Larsen, Niels H. Heegaard, Karsten Buschard

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Comparison of Antioxidant and Alpha-Glucosidase Inhibitory Properties of Moringa peregrina and Ferulago carduchorum Leaf Extracts and Microencapsulation of Superior Plant by Shiva Nouri, Maryam Moslehishad, Seyede Marzieh Hosseini, Mina Etemadi

“…The results showed that the ABTS•+ radical scavenging and α-glucosidase enzyme inhibitory activities of both plants were dependent on extract concentration. …”
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Piezoelectric biosensor with dissipation monitoring enables the analysis of bacterial lytic agent activity by Radka Obořilová, Eliška Kučerová, Tibor Botka, Hana Vaisocherová-Lísalová, Petr Skládal, Zdeněk Farka

“…The sensor, a QCM chip modified with the bacterium S. aureus RN4220 ΔtarM, was utilized to monitor the activity of the enzyme lysostaphin and the phage P68 as model lytic agents. …”
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Structural tailoring of etoricoxib: A spectrochemical, medicinal and pharmacological study by Bakul Akter, Silvia Aishee, Abdullah Hridoy, Md. Mehedi Hasan Pulok, Mohammad Ariful Islam, Antu Biswas, Aurna Patwary, Majedul Hoque, MD. Sazidul Islam, Md. Nipatul Hasan Nirob, Faisal Islam Chowdhury, Monir Uzzaman

“…Etoricoxib (ETC), a selective cyclooxygenase enzyme (COX-2) inhibitor, is widely utilized to manage pain and inflammation. …”
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Digestion, faeces microbiome, and selected blood parameters in dogs fed extruded food containing Black soldier fly (Hermetia illucens) meal by Kanber Kara, Oğuzhan Kahraman, Fatma İnal, Zekeriya Safa İnanç, Sena Yilmaz Öztaş, M. Selçuk Alataş, Ibrar Ahmed

“…As a result, HI larva meal can be used up to 100% instead of chicken meal in extruded dog food without any negative effects on body weight, digestibility, faecal score, consumption preference, kidney-liver enzyme activities and immunological parameters. Besides, HI larva meal at 7.5% and 15% in extruded dog food had different effects on the faecal microbiome.…”
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Mechanical Activation of cPLA2 Impedes Fatty Acid β‐Oxidation in Vein Grafts by Linwei Fan, Yuanjun Tang, Jian Liu, Yueqi Liu, Yiwei Xu, Jiayu Liu, Han Liu, Wei Pang, Yuxuan Guo, Weijuan Yao, Tao Zhang, Qin Peng, Jing Zhou

“…Metabolomic analysis showed that a 15%–1 Hz arterial cyclic stretch, compared to a 5%–1 Hz venous stretch, increased long‐chain fatty acids in VSMCs. cPLA2, identified as a mechanoresponsive molecule, produces excessive arachidonic acid (ArAc) under the 15%–1 Hz stretch, inhibiting CPT1B expression, a key enzyme in fatty acid β‐oxidation. ArAc promotes transcription factor YY1 degradation, downregulating CPT1B. …”
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Genetic Deletion and Pharmacological Inhibition of PI3Kγ Reduces Neutrophilic Airway Inflammation and Lung Damage in Mice with Cystic Fibrosis-Like Lung Disease by Maria Galluzzo, Elisa Ciraolo, Monica Lucattelli, Eriola Hoxha, Martina Ulrich, Carlo Cosimo Campa, Giuseppe Lungarella, Gerd Doring, Zhe Zhou-Suckow, Marcus Mall, Emilio Hirsch, Virginia De Rose

“…Treatment with the PI3Kγ inhibitor AS-605240 decreased the number of neutrophils in BALF of βENaC-Tg mice, reproducing the effect observed with genetic deletion of the enzyme. Conclusions. These results demonstrate the biological efficacy of both genetic deletion and pharmacological inhibition of PI3Kγ in reducing chronic neutrophilic inflammation in CF-like lung disease in vivo.…”
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Expression of Angiogenesis Regulatory Proteins and Epithelial-Mesenchymal Transition Factors in Platelets of the Breast Cancer Patients by Hui Han, Fang-Li Cao, Bao-Zhong Wang, Xue-Ru Mu, Guang-Yao Li, Xiu-Wen Wang

“…Vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), thrombospondin-1 (TSP-1), platelet factor 4 (PF4), and transforming growth factor-β1 (TGF-β1) were measured by enzyme-linked immunosorbent assay. Angiogenesis-associated expression of VEGF (2.1 pg/106 platelets versus 0.9 pg/106 platelets, P < 0.001), PF4 (21.2 ng/106 platelets versus 10.2 ng/106 platelets, P < 0.001), PDGF-BB (42.9 pg/106 platelets versus 19.1 pg/106 platelets, P < 0.001), and TGF-β1 (15.3 ng/106 platelets versus 4.3 ng/106 platelets, P < 0.001) differed in the PP samples of cancer and control subjects. …”
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Effects of early neonatal proinflammatory stress on the expression of BDNF transcripts in the brain regions of prepubertal male rats by D. I. Peregud, S. V. Freiman, A. O. Tishkina, L. S. Sokhranyaeva, N. A. Lazareva, M. V. Onufriev, M. Y. Stepanichev, N. V. Gulyaeva

“…BDNF polypeptide concentration was estimated by means of an enzyme-linked immunosorbent assay, while quantitative polymerase chain reaction followed by reverse transcription was used to detect exon-specific BDNF mRNA expression. …”
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The RNA M5C methyltransferase NSUN2 promotes progression of hepatocellular carcinoma by enhancing PKM2-mediated glycolysis by Qin Qi, Rui Zhong, Yan Huang, Yong Tang, Xiao-wen Zhang, Chang Liu, Chun-fang Gao, Lin Zhou, Jian Yu, Lu-yi Wu

“…Mechanistically, our data revealed that PKM2, a terminal enzyme in the glycolytic pathway, is a downstream target of NSUN2-mediated m5C modification. …”
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Growth performance, intestinal health, and non-specific immunity were significantly affected by feeding different compound lactic acid bacteria supplementation in sea urchin (Stron... by Yuntian Zhang, Yuntian Zhang, Rongwei Zhang, Rongwei Zhang, Yi Chen, Yi Chen, Zhixu Guo, Zhixu Guo, Xiangyu Meng, Xiangyu Meng, Yuzhe Han, Yuzhe Han, Xiaoran Zhao, Xiaoran Zhao, Tongjun Ren, Tongjun Ren

“…Notably, compound lactic acid bacteria improved digestive enzyme activities in the intestine and reduced the feed conversion ratio (FCR). …”
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The sulfur-related metabolic status of Aspergillus fumigatus during infection reveals cytosolic serine hydroxymethyltransferase as a promising antifungal target by Reem Alharthi, Monica Sueiro-Olivares, Isabelle Storer, Hajer Bin Shuraym, Jennifer Scott, Reem Al-Shidhani, Rachael Fortune-Grant, Elaine Bignell, Lydia Tabernero, Michael Bromley, Can Zhao, Jorge Amich

“…We further showed that the structure of the ligand-binding pocket of the fungal enzyme differs significantly from its human counterpart, suggesting that specific inhibitors can be designed. …”
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Phenolic Compounds from Water-Ethanol Extracts of Tetrapleura tetraptera Produced in Cameroon, as Potential Protectors against In Vivo CCl4-Induced Liver Injuries by Peter William Kemewele Saague, Bruno Moukette Moukette, Jacques Romain Njimou, Prosper Cabral Nya Biapa, Francine Nzufo Tankeu, Vicky Joseline Ama Moor, Constant Anatole Pieme, Jeanne Yonkeu Ngogang

“…In rats treated with the extract of T. tetraptera, in a dose-dependent manner, the levels of hepatotoxicity markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) significantly increased while the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and the level of reduced glutathione (GHS) significantly increased compared to the control group. …”
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Scurvy as a Cause of Leg Pain and Limp in Pediatric and Adolescent Patients by Christopher J. DeFrancesco, MD, David Kell, BA, Sean Owens, BS, Matt Grady, MD, Naomi Brown, MD

“…Vitamin C deficiency (VCD) results in collagen dysfunction due to decreased lysyl and prolyl hydroxylase enzyme activity. The resulting disease, known as scurvy, can present with various findings, including gingival bleeding, bruising, other skin lesions, poor wound healing, and tooth and bone abnormalities. …”
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Aggravating Effects of Psychological Stress on Ligature-Induced Periodontitis via the Involvement of Local Oxidative Damage and NF-κB Activation by Qiang Li, Yajuan Zhao, Daokun Deng, Jiuhui Yang, Yongjin Chen, Jia Liu, Min Zhang

“…The combination of CUMS and periodontitis had synergistic effects on increasing hypoxia-inducible factor-1α (HIF-1α) protein expression and reactive oxygen species (ROS) and malondialdehyde (MDA) contents and decreasing antioxidant enzyme activities compared with those in the stress or periodontitis groups. …”
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Transcriptional Regulation of Δ6-Desaturase by Peroxisome Proliferative-Activated Receptor δ Agonist in Human Pancreatic Cancer Cells: Role of MEK/ERK1/2 Pathway by Maryam Darabi, Shima Byagowi, Shabnam Fayezi, Masoud Darabi, Shahab Mirshahvaladi, Mehdi Sahmani

“…The Δ6-desaturase (Δ6D), also known as fatty acid desaturase 2, is a regulatory enzyme in de novo fatty acid synthesis, which has been linked to obesity and diabetes. …”
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