Evaluating the effect of virgin coconut oil pulling on viral load, bacterial load and inflammatory mediator levels in chronic periodontitis – A clinical study by Monika M, Rudhra K, Vennila K, Elanchezhiyan, Sri Harini P, S. Shakeron

“…Both pre- and post-treatment samples were subjected to real-time PCR (rtPCR) and enzyme-linked immunosorbent assay (ELISA) to quantify viral and bacterial loads, as well as levels of key inflammatory mediators. …”
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Diethyldithiocarbamate Delayed Wound-Induced Callus Formation in Potato Tubers by Inhibiting Superoxide Dismutase by CHENG Xinyan, SONG Bingfang, LIU Ning, TIAN Wenmao, YIN Yan, LI Yongcai, BI Yang, WANG Yi

“…We determined the StSODs gene expression and activity of SOD, reactive oxygen species (ROS) content, phenylpropanoid metabolism-related enzyme activity, total phenols and lignin contents at the wound site during the healing process. …”
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MicroRNA-155 Participates in the Expression of LSD1 and Proinflammatory Cytokines in Rheumatoid Synovial Cells by Ziliang Yu, Hao Liu, Jianbo Fan, Feihu Chen, Wei Liu

“…Moreover, RT-qPCR was used to explore the relative LSD1 miRNA expression in FLS when stimulated by miRNA-155 molecules, and Western blot and immunofluorescence assay were applied to probe into the expression level of LSD1. Finally, enzyme-linked immunosorbent assay was employed to analyze the secreting level of proinflammatory cytokines in FLS when stimulated by miRNA-155 molecules. …”
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Changes in Circulating BDNF in relation to Sex, Diet, and Exercise: A 12-Week Randomized Controlled Study in Overweight and Obese Participants by M. Glud, T. Christiansen, L. H. Larsen, B. Richelsen, J. M. Bruun

“…Using a previously published 12-week randomized study, serum BDNF was assessed at baseline and after 12 weeks using an enzyme-linked immunosorbent assay method. Fifty overweight or obese but healthy individuals (26 women, mean age of 36.4 ± 7.9 years; 24 men, mean age of 38.0 ± 5.9 years) were included and allocated into three groups: exercise-only (EXO; 12 weeks of aerobic exercise and isocaloric diet), diet-only (DIO; 8 weeks of very low energy diet (VLED 600 kcal/day) followed by a 4-week weight maintenance diet), or diet and exercise (DEX; 12 weeks of aerobic exercise in parallel with 8 weeks of VLED (800 kcal/day) followed by a 4-week weight maintenance diet). …”
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Antisense oligonucleotides for the arterial hypertension mechanisms study and therapy by L. O. Klimov, A. A. Seryapina, V. F. Zarytova, A. S. Levina, A. L. Markel

“…The review discusses the results of the use of titanium dioxide (TiO2) containing antisense nanocomposites for the angiotensin converting enzyme in rats with stress induced arterial hypertension (ISIAH). …”
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KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) by Aino Komatsu, Mizuki Fujibayashi, Kazato Kumagai, Hidemasa Suzuki, Yuki Hata, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Junko Kyozuka

“…We find that KAI2-dependent signaling positively controls the expression of MpLONELY GUY (MpLOG), encoding a cytokinin biosynthesis enzyme. Disruption of the MpLOG function decreases endogenous cytokinin levels and causes defects similar to KAI2-dependent signaling mutants. …”
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Biomarkers of Oxidative Stress in Huntington's Disease and Other Neurological Disorders: a Comparative Study by Marisol Peña Sánchez, Gabriel Andrés Peña de los Santos, Gretel Riverón Forment, Gloria Lara Fernández, Tatiana Acosta Sánchez, Alina González-Quevedo Monteagudo

“…<br /><strong>Results:</strong> there were significant differences in malonyldialdehyde in Huntington's disease compared to the control (p=0.02), but not with the rest of the groups. The enzyme superoxide dismutase in Huntington's disease was statistically lower compared to amyotrophic lateral sclerosis, although for catalase it was higher in relation to the rest of the patients. …”
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Emodin promotes the recovery of rheumatoid arthritis by regulating the crosstalk between macrophage subsets and synovial fibroblast subsets by Lianying Cheng, Xiaofeng Rong

“…In vitro experimental methods such as enzyme linked immunosorbent assay (ELISA), Western blotting, Transwell assays, CCK‐8 assays and cell coculture were used to assess cell proliferation, migration and secretion of inflammatory factors. …”
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Analysis of Phenolic and Flavonoid Content, α-Amylase Inhibitory and Free Radical Scavenging Activities of Some Medicinal Plants by Lalit Kala Pandey, Khaga Raj Sharma

“…The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the antioxidant potential. The α-amylase enzyme inhibition activity was performed to evaluate the antidiabetic activity of plant extracts. …”
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Optimization of Extraction of Compound Flavonoids from Chinese Herbal Medicines Based on Quantification Theory and Evaluation of Their Antioxidant Activity by Xu Yang, Dongli Cao, Haiyu Ji, Yingying Feng, Juan Yu, Anjun Liu

“…The highest CFs yield was obtained under the following extraction condition: liquid-solid ratio of 35 : 1 mL/g, extraction temperature of 75°C, extraction duration of 75 min, and extraction mode enzyme-assisted extraction. The forecasted yield was 37.62%. …”
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Long-term cardiac MRI follow up of MANTICORE (Multidisciplinary Approach to Novel Therapies in Cardio-Oncology REsearch) by Dina Labib, Mark Haykowsky, Emer Sonnex, John R. Mackey, Richard B. Thompson, D. Ian Paterson, Edith Pituskin

“…We extend the original MANTICORE trial which showed that angiotensin-converting enzyme inhibitors (ACEI) and beta-blockers (BB) could mitigate the decline in left ventricular (LV) ejection fraction (EF) during the first year of trastuzumab treatment. …”
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Seroprevalence of Seven Reproductive Diseases in Beef and Dairy Cows from Three Provinces in Indonesia by Didik Tulus Subekti, Mira Fatmawati, Arie Khoiriyah, Arum Pramesthi, Sulinawati Fong, Muhammad Ibrahim Desem, Zul Azmi, Eni Kusumaningtyas, Dwi Endrawati, Eko Setyo Purwanto

“…The samples were then sent to Indonesian Research Centre for Veterinary Science (IRCVS) for further detection of seven reproductive diseases by enzyme-linked immunosorbent assay (ELISA). Seven reproductive diseases to be tested in parallel are neosporosis, chlamydiosis, brucellosis, Q fever, bovine viral diarrhea (BVD), infectious bovine rhinotracheitis (IBR), and BHV-4 infection. …”
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Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway by Woan Sean Tan, Palanisamy Arulselvan, Govindarajan Karthivashan, Sharida Fakurazi

“…Nitric oxide (NO) production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay (ELISA) and immunoblotting. …”
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α-Lipoic Acid Inhibits Helicobacter pylori-Induced Oncogene Expression and Hyperproliferation by Suppressing the Activation of NADPH Oxidase in Gastric Epithelial Cells by Eunyoung Byun, Joo Weon Lim, Jung Mogg Kim, Hyeyoung Kim

“…The aim of this study is to investigate the effect of α-LA on H. pylori-induced hyperproliferation and oncogene expression in gastric epithelial AGS cells by determining cell proliferation (viable cell numbers, thymidine incorporation), levels of reactive oxygen species (ROS), NADPH oxidase activation (enzyme activity, subcellular levels of NADPH oxidase subunits), activation of redox-sensitive transcription factors (NF-κB, AP-1), expression of oncogenes (β-catenin, c-myc), and nuclear localization of β-catenin. …”
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Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria by donya kazemi, abbas doosti, mostafa Shakhsi niaee

“…Conclusion: The results of PCR, restricted enzyme digestion, and sequencing by GENEray revealed that BAB1-0278 was cloned correctly in pNZ8148-Usp45. …”
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Neuroprotective Effects of Collagen-Glycosaminoglycan Matrix Implantation following Surgical Brain Injury by Jia-Hui Chen, Wei-Cherng Hsu, Kuo-Feng Huang, Chih-Huang Hung

“…Proinflammatory (tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells)) and anti-inflammatory (IL-10 and granulocyte-macrophage colony-stimulating factor (GMCSF)) cytokine expression was evaluated by enzyme-linked immunosorbent assays. Microglial activation was evaluated by immunohistochemistry, and the neuroprotective effect of CG matrix implantation was evaluated by an immunohistochemical study of microglia ED-1 and IBA-1 (activated microglia) and myeloperoxidase (MPO) and by the analysis of IL-6, IL-10, TNF-α, NF-κB, and GMCSF cytokine levels. …”
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Effect of Bacillus subtilis JZXJ-7, a Strain Isolated from Shrimp Paste, on Colonic Function and Microbial Community in Mice Infected with Vibrio parahaemolyticus by ZHOU Kui, WANG Rundong, GONG Mengxue, MOU Jingyuan, DENG Yijia, LI Xuepeng, ZHANG Yuhao, LI Jianrong

“…Moreover, high-dose JZXJ-7 significantly inhibited cytokine-mediated inflammation, increased antioxidant enzyme activities and enhanced intestinal permeability. …”
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Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection by Niharika Bala, Ramish H. Rafay, Sarah C. Glover, Abdel A. Alli

“…The identified proteins were predominantly associated with cellular membranes and with function of the cytoskeleton, enzyme regulation, and signal transduction. <b>Conclusions:</b> Taken together, our data identify novel urinary biomarkers that could be used to further investigate the long-term effects of SARS-CoV-2 infection.…”
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Salivary Fractalkine Differentiating Periodontitis from Periodontally Healthy Subjects by Omer Mohammed Harbood, Raghad Fadhil Abbas

“…After each subject’s saliva was collected, periodontal markers including bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment loss (CAL) were recorded. Enzyme-linked immunosorbent assays (ELISA) were used to detect the protein levels of salivary CX3CL1 and CX3CR1. …”
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Target miRNA identification for the LPL gene in large yellow croaker (Larimichthys crocea) by Kalim Ullah, Aslam Hossain, Mingyue Cao, Liangyi Xue, Yajun Wang

“…Subsequent in-depth analysis further implicated these miRNAs, along with miR-891a, as prospective targets of LPL, suggesting their integral involvement in the regulation of this critical enzyme. Validation of these bioinformatics predictions was conducted through the construction of double luciferase reporters concealing the LPL 3′ untranslated region (3′UTR), substantiating that miR-84a and miR-1231-5p can modulate LPL expression via the LPL 3′UTR. …”
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