Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis by Ying Zhang, Rong Cao, Haijian Ying, Juping Du, Shuaishuai Chen, Na Wang, Bo Shen

“…TLR10 levels in B cells and B cell subsets (CD19+CD27+, CD19+CD27−, CD27+IgD−, CD27+IgD+, CD27−IgD+, D27−IgD−, CD19+CD5+, and CD19+CD5−) and inflammatory biomarker concentrations in peripheral blood (PB) obtained from RA subjects and HCs were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. …”
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Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells by Ziwei Lin, Yanlong Zhou, Ruiwen Chen, Qiuyan Tao, Qiwen Lu, Qianchao Xu, Haibin Yu, Ping Jiang, Zhihui Zhao

“…COS treatment also suppressed excessive reactive oxygen species (ROS) production and restored antioxidant enzyme activity under LPS-induced oxidative stress conditions. …”
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The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon by Lu Zou, Dan Cao, Qing Sun, Wenjun Yu, Bingzong Li, Guoqiang Xu, Liang Zhou

“…Biochemical experiments found that the CRBN-interacting protein KDM5C could stabilize CRBN and enhance the antileukemia effect of Len in an enzyme activity-independent manner. Furthermore, our studies revealed that the small-molecule compound MLN4924 could increase CRBN by elevating KDM5C.The combination of MLN4924 and Len can further increase the sensitivity of primary AML cells and AML cell lines to Len. …”
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4,6-Disubstituted pyrimidine-based microtubule affinity-regulating kinase 4 (MARK4) inhibitors: synthesis, characterization, in-vitro activity and in-silico studies by Ashanul Haque, Khalaf M. Alenezi, Mohd. Saeed Maulana Abdul Rasheed, Md. Ataur Rahman, Saleha Anwar, Shahzaib Ahamad, Dinesh Gupta

“…ATPase inhibition was carried out against the MARK4 enzyme. Molecular docking and molecular dynamics (MD) simulation at 500 ns was carried out against MARK4 (PDB: 5ES1). …”
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Association of fatty acid desaturase 2 gene polymorphism (rs28456) with susceptibility to bipolar disorder in the Turkish population: A case-control study by Pirim Dilek, Gümüş Ceren, Boztepe Esra, Akdağ Emine Merve, Şirin Hande

“…The FADS2 gene encodes a key, rate-limiting enzyme involved in polyunsaturated fatty acid (PUFA) metabolism. …”
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Optimization of Enzymatic Hydrolysis Process, Structural Characterization and Antioxidant Activity Analysis of Gastrodia elata Polysaccharide by Yunfei HU, De ZHOU, Qinglan ZENG, Jinsong HE, Ming ZHAO, Qingyan TANG

“…Results revealed that the optimal enzymolysis process was as follows: enzyme dosage, 460 U/g, enzymolysis time, 100 min, enzymolysis temperature, 60 ℃, enzymolysis pH, 5.4. …”
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Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice by Guangshan Zhao, Jianyuan Teng, Ruixia Dong, Qiuyan Ban, Lian Yang, Kang Du, Yifei Wang, Hanlin Pu, Chung S. Yang, Zhe Ren

“…The underlying mechanisms involve downregulation of gluconeogenesis (lower protein levels of TXNIP and FBP and enzyme activity of FBP), upregulation of lipid catabolism (higher protein levels of CPT-1α and PPARα), downregulation of lipogenesis (lower protein level of SREBP-1), and modification of the structure and abundance of gut microbiota to modulate metabolic homeostasis. …”
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Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model by Toshio Miki, Ludivina Vazquez, Lisa Yanuaria, Omar Lopez, Irving M. Garcia, Kazuo Ohashi, Natalie S. Rodriguez

“…Mucopolysaccharidosis type 1 (MPS-1), also known as Hurler’s disease, is a congenital metabolic disorder caused by a mutation in the alpha-L-iduronidase (IDUA) gene, which results in the loss of lysosomal enzyme function for the degradation of glycosaminoglycans. …”
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Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats by Yonghua Zong, Mingxiao Zhang, Shuai Li, Wenqian Qi, Juan Li, Tonghua Liu, Huijun Yang, Chen Lu, Xiaosong Hu

“…The results suggested that EP administration could effectively inhibit the liver fibrosis induced by BDL in rat, which may be associated with the enhanced activity of Nrf2 to mediate antioxidant enzyme system and downregulate the inflammatory genes.…”
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Lipid Mediator Informatics and Proteomics in Inflammation-Resolution by Yan Lu, Song Hong, Katherine Gotlinger, Charles Serhan

“…LM informatics employing liquid chromatography-ultraviolet-tandem mass spectrometry (LC-UV-MS/MS), gas chromatography-mass spectrometry (GC-MS), computer-based automated systems equipped with databases and novel searching algorithms, and enzyme-linked immunosorbent assay (ELISA) to evaluate and profile temporal and spatial production of mediators combined with proteomics at defined points during experimental inflammation and its resolution enable us to identify novel mediators in resolution. …”
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Influence of multimodal low-opioid anesthesia on the dynamics of аnnexin V in blood plasma during cardiac surgery by S.R. Maruniak, O.A. Loskutov, O.M. Druzhyna, I.R. Malish, N.V. Korotchuk

“…The determination of the level of аnnexin V in the blood was carried out before CPB and after bringing the sternum by enzyme-linked immunosorbent assay. Results. The low-opioid scheme of anesthetic management was associated with significantly (by 1.5 times) lower level of аnnexin V and by 28.38 % lower level of interleukin-6 at the end of the surgery as compared to the standard scheme. …”
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Heterologous Expression and Functional Analysis of <i>Exiguobacterium</i> Algin Lyase Gene by <i>Pichia pastoris</i> by Hanwen Wu, Kai Hou, Yutong Jiang, Mingjian Luan, Yuxia Sun, Xi He, Xiangzhong Zhao

“…The activity of the recombinant enzyme in 5 L high-density fermentation reached 1306 U/mg protein, 3.9 times that of the original <i>Exiguobacterium</i> strain. …”
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Fatal Renal Failure in a Spinal Cord Injury Patient with Vesicoureteric Reflux Who Underwent Repeated Ureteric Reimplantations Unsuccessfully: Treatment Should Focus on Abolition o... by Subramanian Vaidyanathan, Bakul Soni, Kottarathil Abraham Abraham, Peter Hughes, Gurpreet Singh

“…Angiotensin-converting enzyme inhibitors or angiotensin-receptor-blocking agents should be prescribed even in the absence of hypertension when a spinal cord injury patient develops vesicoureteral reflux and proteinuria.…”
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Increase in methemoglobin fraction due to the use of glyceryl trinitrate patches in preterm infants: a case report and literature review by Andrea Calandrino, Andrea Calandrino, Francesco Vinci, Francesco Vinci, Marcella Battaglini, Marcella Battaglini, Samuele Caruggi, Samuele Caruggi, Chiara Andreato, Chiara Andreato, Carolina Montobbio, Marianna Vannati, Diego Minghetti, Giorgia Brigati, Luca Antonio Ramenghi, Luca Antonio Ramenghi

“…Elevated MeHb levels, noted in similar cases here reviewed, can impair oxygen exchange, especially in vulnerable preterm infants with immature skin and reduced enzyme activity.ConclusionThis case highlights the need for careful MeHb monitoring and a multidisciplinary approach to manage ischemia safely in neonates undergoing GTN therapy.…”
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Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis by Lixin Zhang, Xianling Ning, Jingyi Cui, Yanqiang Zhao, Fei Xie, Ningxin Zhu, Dan Wang, Man Qin, Zhiqiang Lin, Yuanyuan Wang

“…By using poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide)-based triblock copolymer (PPP) as a scaffold to carry Prussian blue nanoparticles (PBNPs), with effective ROS scavenging capability via multiple enzyme-like activities, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)activities. …”
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Preventive Effect of Salicylate and Pyridoxamine on Diabetic Nephropathy by Tarek Kamal Abouzed, Seiichi Munesue, Ai Harashima, Yusuke Masuo, Yukio Kato, Khaled Khailo, Hiroshi Yamamoto, Yasuhiko Yamamoto

“…Attenuation of macrophage infiltration into the kidneys and upregulation of antiglycating enzyme glyoxalase 1 gene expression were found only in the salicylate treatment group. …”
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Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices by Luna Shrestha, Boris Kulig, Roberto Moscetti, Riccardo Massantini, Elke Pawelzik, Oliver Hensel, Barbara Sturm

“…Hence, the results demonstrated the feasibility of HSI as an indirect alternative to the standard chemical analysis of PPO enzyme activity.…”
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Modifiable life style factors and male reproductive health: a cross-sectional study in IVF clinic attendees in Ghana by Brodrick Yeboah Amoah, Saliah Yao Bayamina, Cosmos Gborsong, Hubert Owusu, George Awuku Asare, Emmanuel Kwabena Yeboah, Josephine Ablakwa, Georgina Hammond

“…Hormonal profiles (LH, FSH, testosterone, estradiol) were measured using the enzyme-linked fluorescent assay (ELFA). Statistical analyses included Pearson's product-moment correlation and Bonferroni correction.ResultsSmoking and psychological stress were significantly associated with reduced sperm motility, viability, and concentration (p &lt; 0.05). …”
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Probiotic active gel promotes diabetic wound healing through continuous local glucose consumption and antioxidant by Yingshuai Wang, Lihua Shi, Junna Lu, Fengyuan Wang, Zihan Zhou, Yuexuan Wang, Xiangyu Du, Di Qin, Fangman Chen, Dan Shao, Yuanyuan Gao, Cheng Gao, Tongyi Sun

“…LR&AB@CAH reduces excess ROS, decreases oxidative substances, and boosts antioxidant enzyme activity. In a diabetic wound mouse model, it functions as a glucose scavenger and antioxidant, reducing ROS and supporting wound healing. …”
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Functional Involvement of Melatonin and Its Receptors in Reproductive Regulation of the Marine Teleost, Large Yellow Croaker (<i>Larimichthys crocea</i>) by Xudong Liang, Jixiu Wang, Baoyi Huang, Haojie Yuan, Yucheng Ren, Chenqian Wu, Tianming Wang, Jingwen Yang

“…Moreover, our data indicate that melatonin and its receptors are primarily expressed during stage III, highlighting their role in sexual maturity. Enzyme- linked immunosorbent assay (ELISA) results further demonstrated that in vitro melatonin incubation in the brain of <i>L. crocea</i> influenced gonadotropin-releasing hormone (GnRH) and testosterone secretion in a dose-dependent manner, suggesting actions beyond the classical hypothalamic–pituitary–gonadal (HPG) axis. …”
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