Simultaneous exposure to microplastics and heavy metal lead induces oxidative stress, histopathological damage, and immune dysfunction in marine mussel Mytilus coruscus by Chuanyue Chen, Xueer Wang, Yingrong Jin, Yinchang Yao, Suchang Li, Zhi Liao, Xiaolin Zhang, Xiaojun Yan

“…In addition, co-exposure induced a higher level of oxidative stress, which was reflected by an increase in hydrogen peroxide and malondialdehyde content, and a decrease in antioxidant enzyme activity. Meanwhile, co-exposure poses a significant threat to the immune function of the mussels, as evidenced by induction of hemocytes to produce excess reactive oxygen species (ROS), significantly reducing lysosome activity (p < 0.05), inhibiting the expression of autophagy-related genes, and inducing the expression of apoptosis-related genes, resulting in hemocyte apoptosis. …”
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Surface topography modulates initial platelet adhesion to titanium substrata by Cecilia Yan Guo, Raymond Mo, Hugh Kim

“…In addition, the secretion of critical molecules stored in platelet granules (platelet factor 4, PF4; soluble P-selectin, sCD62P; transforming growth factor-beta1, TGF-β1; platelet-derived growth factor-AB, PDGF-AB) was measured by enzyme-linked immunosorbent assay (ELISA) analysis of the supernatants. …”
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Pharmacophore-based approach for the identification of prospective UDP-2,3-diacylglucosamine hydrolase (LpxH) inhibitor from natural product database against Salmonella Typhi by Divyapriya Karthikeyan, Sanjit Kumar, NS Jayaprakash

“…To address this, ligand-based pharmacophore modeling was used to identify potential inhibitors of the S. typhi LpxH protein, a crucial enzyme in the lipid A biosynthesis pathway. A natural compound library of 852,445 molecules was screened against a pharmacophore model developed from known LpxH inhibitors. …”
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‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…The ligation efficiency was significantly higher because the restriction enzyme sites utilized were removed from the vector after being successfully constructed. …”
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Consequences of the Lack of CD73 and Prostatic Acid Phosphatase in the Lymphoid Organs by Gennady G. Yegutkin, Kaisa Auvinen, Marika Karikoski, Pia Rantakari, Heidi Gerke, Kati Elima, Mikael Maksimow, Ileana B. Quintero, Pirkko Vihko, Marko Salmi, Sirpa Jalkanen

“…CD73, ecto-5′-nucleotidase, is the key enzyme catalyzing the conversion of extracellular AMP to adenosine that controls vascular permeability and immunosuppression. …”
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Aqueous Levels of Pigment Epithelium-Derived Factor and Macular Choroidal Thickness in High Myopia by Wei Chen, Yubo Guan, Guanghui He, Zhiwei Li, Hui Song, Shiyong Xie, Quanhong Han

“…Serum and aqueous levels of PEDF were measured by enzyme-linked immunosorbent assay in 36 high myopia patients (36 eyes) with no CNV (non-CNV group), 14 high myopia patients (14 eyes) with CNV (CNV group), and 42 nonmyopia patients (42 eyes) (control group). …”
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Optimization of a fermented pumpkin-based beverage to improve Lactobacillus mali survival and α-glucosidase inhibitory activity: A response surface methodology approach by W.Y. Koh, U. Uthumporn, A. Rosma, A.R. Irfan, Y.H. Park

“…Relationship between fermentation temperature, inoculum and ingredient concentration with response variables (fermentation time at the fermentation endpoint pH 4.5, survival rate of Lactobacillus mali K8 in pumpkin-based beverage treated with simulated gastrointestinal tract enzyme fluids, α-glucosidase inhibitory activity and sensory overall acceptability after 4 weeks of refrigerated storage) was investigated using response surface methodology. …”
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A novel platform for engineered AAV-based vaccines by Sabrina Babutzka, Miranda Gehrke, Anastasia Papadopoulou, Maria Diedrichs-Möhring, Maria Giannaki, Lena Hennis, Bastian Föhr, Cale Kooyman, Andreas Osterman, Evangelia Yannaki, Gerhild Wildner, Hermann Ammer, Stylianos Michalakis

“…This engineering endowed AAV.RBDs with SARS-CoV-2-like properties, such as angiotensin-converting enzyme 2 receptor affinity. In line with this, AAV.RBDs were neutralized by sera from human donors vaccinated against SARS-CoV-2. …”
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RNA N4‐acetylcytidine modification and its role in health and diseases by Qiang Wang, Yixiao Yuan, Qiang Zhou, YingDong Jia, Jing Liu, GuangJun Xiao, Chunhong Li, Xiulin Jiang

“…This review first outlines the key regulatory enzyme mediating ac4C modification, N‐acetyltransferase 10 (NAT10), including its critical roles in regulating RNA stability, transcriptional efficiency, and translational fidelity. …”
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GGCX promotes Eurasian avian-like H1N1 swine influenza virus adaption to interspecies receptor binding by Jiahui Zou, Meijun Jiang, Rong Xiao, Huimin Sun, Hailong Liu, Thomas Peacock, Shaoyu Tu, Tong Chen, Jinli Guo, Yaxin Zhao, Wendy Barclay, Shengsong Xie, Hongbo Zhou

“…In this study, we conduct a comprehensive genome-wide CRISPR/Cas9 knockout screen utilizing EA H1N1 SIV in porcine kidney cells. Knocking out the enzyme gamma glutamyl carboxylase (GGCX) reduces virus replication in vitro and in vivo by inhibiting the carboxylation modification of viral haemagglutinin (HA) and the adhesion of progeny viruses, ultimately impeding the replication of EA H1N1 SIV. …”
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Leptin Regulates Proliferation and Apoptosis in Human Prostate by Eduardo Leze, Jorge Luiz Alves-Pereira, Sicilia Colli, Fernanda Silveira Cavalcante, Francisco José Sampaio, Cristiane da Fonte Ramos

“…This paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue. …”
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The Levels of Tumor Necrosis Factor-Alpha and Interleukin-6 in Patients with Isolated Coronary Artery Ectasia by Mustafa Aydin, Ishak Ozel Tekin, Sait Mesut Dogan, Nesligul Yildirim, Mehmet Arasli, Muhammet Rasit Sayin, Ziyaettin Aktop

“…TNF-𝛼 and IL-6 concentrations in blood were assesed by enzyme-linked immunosorbent assay (ELISA). Results. …”
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Date pomace polysaccharides-capped selenium nanoparticles: Biosynthesis, optimization, physicochemical characterization, biological activities, stability and gut microbiota modulat... by Gafar Babatunde Bamigbade, Athira Subhash, Mohammed Abdin, Hebah Jarusheh, Basim Abu-Jdayil, Shao-Quan Liu, Giovanni Palmisano, Abdelmoneim Ali, Afaf Kamal Eldin, Mutamed Ayyash

“…They also showed concentration-dependent enzyme inhibition: 86.8 % α-amylase, 53.9 % α-glucosidase, and 42.4 % ACE inhibition at 100 mg/L, along with anticancer effects against Caco-2 (32.3 %) and MCF-7 (11.3 %) at 50 mg/L. …”
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Antioxidant and anti-diabetic potential of the green synthesized silver nanoparticles using Martynia annua L. root extract by Megha B. Abbigeri, Bothe Thokchom, Sapam Riches Singh, Santosh Mallikarjun Bhavi, B.P. Harini, Ramesh Babu Yarajarla

“…They exhibited significant inhibition of alpha amylase, a pivotal enzyme in carbohydrate metabolism, suggesting their efficacy as anti-diabetic agents. …”
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Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway by Yuqin Ye, Yongxiang Yang, Chen Chen, Ze Li, Yanfeng Jia, Xinhong Su, Chaoxian Wang, Xiaosheng He

“…The hippocampal level of TLR4 and downstream molecules and inflammatory cytokines were, respectively, detected by Western blot and enzyme-linked immunosorbent assay. EA enhanced hippocampal neurogenesis and inhibited TLR4 expression at 21, 28, and 35 days after TBI, but the beneficial effects of EA on posttraumatic neurogenesis and neurological functions were attenuated by lipopolysaccharide-induced TLR4 activation. …”
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Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney by N. L. Rukavina Mikusic, N. M. Kouyoumdzian, E. Rouvier, M. M. Gironacci, J. E. Toblli, B. E. Fernández, M. R. Choi

“…In addition, hydrocortisone reversed Ang-(1-7)-dopamine overinhibition on the enzyme, suggesting that this inhibition is closely related to Ang-(1-7) stimulation on renal dopamine uptake. …”
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The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation by Malihe Moradzadeh, Sirous Tayebi, Hossein Poustchi, Kourosh Sayehmiri, Parisa Shahnazari, Elnaz Naderi, Ghodratollah Montazeri, Ashraf Mohamadkhani

“…Serum TLR2 was measured by enzyme-linked immunosorbent assay. Interactions of truncated HBeAg and TLR2 proteins were evaluated with molecular docking software. …”
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A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance a... by Ning Gan, Ping Xiong, Ji Wang, Tianhua Li, Futao Hu, Yuting Cao, Lei Zheng

“…The amount of Δf was proportional to (CRP) from 0.003 to 200 ng mL−1 with a low detection limit of 1 pg mL−1. Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag. …”
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Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking by Ya-Li Wang, Guang Hu, Qian Zhang, Yu-Xiu Yang, Qiao-Qiao Li, Yuan-Jia Hu, Hua Chen, Feng-Qing Yang

“…Tyrosinase (TYR) is a rate-limiting enzyme in the synthesis of melanin, while direct TYR inhibitors are a class of important clinical antimelanoma drugs. …”
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Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) by Jonggeun Kim, Bosung Choi, Young-Hwan Park, Byoung-Kwan Cho, Hyoun-Sub Lim, Savithiry Natarajan, Sang-Un Park, Hanhong Bae

“…F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. …”
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